How does a confocal microscope work
... remember this from introductory optics, perhaps you have seen a formula such as 1/s + 1/s' = 1/f for locating the image formed by a lens. Points don't need to be at the focal point of the lens in order for the lens to form an image.) So, we want to just look at the blue point, that is, the point dir ...
... remember this from introductory optics, perhaps you have seen a formula such as 1/s + 1/s' = 1/f for locating the image formed by a lens. Points don't need to be at the focal point of the lens in order for the lens to form an image.) So, we want to just look at the blue point, that is, the point dir ...
Optimization of a fluorescence in situ hybridization method for the
... Epifluorescence microscopy was used to detect the presence of specific fluorescent signals in fungal structures. Confocal laser scanning microscope was utilized to reconstruct the 3D structure of the relatively thick root sections. Since autofluorescence of the plant cell walls hampered signal detec ...
... Epifluorescence microscopy was used to detect the presence of specific fluorescent signals in fungal structures. Confocal laser scanning microscope was utilized to reconstruct the 3D structure of the relatively thick root sections. Since autofluorescence of the plant cell walls hampered signal detec ...
The effect of detector size on the signal-to
... A confocal microscope may be converted into a polarization-sensitive instrument merely by placing a polar in the illumination path and a suitably crossed analyser in the detection path. The confocal microscope is particularly suitable for polarised light studies since it possesses an infinite extinc ...
... A confocal microscope may be converted into a polarization-sensitive instrument merely by placing a polar in the illumination path and a suitably crossed analyser in the detection path. The confocal microscope is particularly suitable for polarised light studies since it possesses an infinite extinc ...
Transmission Electron Microscopy (TEM)
... Laser Scanning Confocal Microscopy Fluorescence technique Uses laser light for excitation Improves image resolution over conventional fluorescence techniques Optically removes out-of-focus light and detects only signal from focal plane Can construct an in-focus image of considerable depth ...
... Laser Scanning Confocal Microscopy Fluorescence technique Uses laser light for excitation Improves image resolution over conventional fluorescence techniques Optically removes out-of-focus light and detects only signal from focal plane Can construct an in-focus image of considerable depth ...
MICROSOPE TYPES PPT
... • Lets light pass through an object and then through two or more lenses. ...
... • Lets light pass through an object and then through two or more lenses. ...
University of Groningen Unraveling structure and dynamics by
... Unraveling structure and dynamics by confocal microscopy diffraction grating. The diffracted light is focused on the rear focal plane of the objective, and the resultant pattern can be viewed as the reciprocal image of the specimen.[3,6] Therefore, the diffraction pattern of a point object when hig ...
... Unraveling structure and dynamics by confocal microscopy diffraction grating. The diffracted light is focused on the rear focal plane of the objective, and the resultant pattern can be viewed as the reciprocal image of the specimen.[3,6] Therefore, the diffraction pattern of a point object when hig ...
Confocal Microscopy
... – 100x / 1.4 NA: resolution = 220nm, so 1 Airy unit = 44 mm – 40x / 1.3 NA: resolution = 235nm, so 1 Airy unit = 19 mm – 20x / 0.75 NA: resolution = 407nm, so 1 Airy unit = 16 mm – 10x / 0.45 NA: resolution = 678nm, so 1 Airy unit = 14 mm ...
... – 100x / 1.4 NA: resolution = 220nm, so 1 Airy unit = 44 mm – 40x / 1.3 NA: resolution = 235nm, so 1 Airy unit = 19 mm – 20x / 0.75 NA: resolution = 407nm, so 1 Airy unit = 16 mm – 10x / 0.45 NA: resolution = 678nm, so 1 Airy unit = 14 mm ...
Diffraction
... A. Introduction to Confocal Microscopy 1. Confocal versus conventional (widefield) fluorescence 2. Optical sectioning 3. Imaging modes and applications 4. Advantages, limitations of confocal ...
... A. Introduction to Confocal Microscopy 1. Confocal versus conventional (widefield) fluorescence 2. Optical sectioning 3. Imaging modes and applications 4. Advantages, limitations of confocal ...
Chapter 4 - microscopy
... Secondary electrons detected by scintillator and PMT – More secondary electrons when beam more parallel to surface – More secondary electrons -> brighter – Thus images have 3D appearance ...
... Secondary electrons detected by scintillator and PMT – More secondary electrons when beam more parallel to surface – More secondary electrons -> brighter – Thus images have 3D appearance ...
The 39 Steps: A Cautionary Tale
... factor of about two more in gain. Beware that PMT black level or (brightness) control permits the addition or subtraction of an arbitrary amount from the signal presented to the digitizer. The black level should be set so that the signal level in the darkest parts of the image is 5-10 digital units. ...
... factor of about two more in gain. Beware that PMT black level or (brightness) control permits the addition or subtraction of an arbitrary amount from the signal presented to the digitizer. The black level should be set so that the signal level in the darkest parts of the image is 5-10 digital units. ...
Optical Imaging Instrument for BioBED - CenSSIS
... - Axial line spread function measurements show that the sectioning is 1.6 – 9.8 mm for detection slits of width 5-100 um under nominal conditions (Figure 7). - Lateral resolution of 0.8 – 2 mm have been measured with 25, 50, 100 mm and no slit widths (Figure 8). Note: Lateral resolution = 5 mm at th ...
... - Axial line spread function measurements show that the sectioning is 1.6 – 9.8 mm for detection slits of width 5-100 um under nominal conditions (Figure 7). - Lateral resolution of 0.8 – 2 mm have been measured with 25, 50, 100 mm and no slit widths (Figure 8). Note: Lateral resolution = 5 mm at th ...
39 Steps
... affects the fraction of the light emitted by the specimen that can be collected. This is also true for light from the laser. Objective magnification is inversely related to the diameter of the objective lens entrance pupil. The objective will only function properly if the entire entrance pupil is fi ...
... affects the fraction of the light emitted by the specimen that can be collected. This is also true for light from the laser. Objective magnification is inversely related to the diameter of the objective lens entrance pupil. The objective will only function properly if the entire entrance pupil is fi ...
Photoacoustic effect applied on cell membranes: Direct observation
... region and emit powerful pressure waves. By using a multi-photon confocal laser microscope, in which we can irradiate the sample with different laser wavelengths, we have directly observed and recorded this effect in human red blood cells and Chinese hamster ovarian cells. At low energy, these mecha ...
... region and emit powerful pressure waves. By using a multi-photon confocal laser microscope, in which we can irradiate the sample with different laser wavelengths, we have directly observed and recorded this effect in human red blood cells and Chinese hamster ovarian cells. At low energy, these mecha ...
Light Microscopy 2
... • Transparent samples • Dead specimen • Must be small enough to fit on a slide ...
... • Transparent samples • Dead specimen • Must be small enough to fit on a slide ...
12. confocal microscopy.
... 12.1.1. Principle. The principle of confocal microscopy was described prior to the invention of lasers [Ref Minsky Patent 1957]. However, today most confocal systems use lasers for illumination. The confocal imaging system can operate either in transmission or reflection, as shown in Fig. 1. In both ...
... 12.1.1. Principle. The principle of confocal microscopy was described prior to the invention of lasers [Ref Minsky Patent 1957]. However, today most confocal systems use lasers for illumination. The confocal imaging system can operate either in transmission or reflection, as shown in Fig. 1. In both ...
Confocal Microscopy Short Course Schedule
... This short course, offered by the Donald Danforth Plant Science Center’s Integrated Microscopy Facility, provides a solid background on the use of light microscopy, especially confocal microscopy, as a tool to study cell and molecular biology. The course emphasizes imaging of plant tissues but it ...
... This short course, offered by the Donald Danforth Plant Science Center’s Integrated Microscopy Facility, provides a solid background on the use of light microscopy, especially confocal microscopy, as a tool to study cell and molecular biology. The course emphasizes imaging of plant tissues but it ...
Olympus Introduces FV1000 Multi
... The Olympus multi-laser combiner has been designed to provide market leading flexibility and functionality within a compact footprint. The system supports up to six diode and gas lasers, enabling the selection of a large number of wavelengths from near UV to far red. Presently, laser diodes are avai ...
... The Olympus multi-laser combiner has been designed to provide market leading flexibility and functionality within a compact footprint. The system supports up to six diode and gas lasers, enabling the selection of a large number of wavelengths from near UV to far red. Presently, laser diodes are avai ...
Microscopes
... • Uses electrons to image the sample • Electromagnets act as lenses (though very weak) • Electron λ ~ 0.0055 nm • Thus resolutions of < 1 nm • WONDERFUL RESOLUTION BUT: ...
... • Uses electrons to image the sample • Electromagnets act as lenses (though very weak) • Electron λ ~ 0.0055 nm • Thus resolutions of < 1 nm • WONDERFUL RESOLUTION BUT: ...
Microscopy - York Technical College
... cells. Although they live in the noses and throats of many people without leading to disease, if they break through into the bloodstream they can cause potentially fatal meningitis and septicemia. (Confocal image by Shao Jin Ong.) ...
... cells. Although they live in the noses and throats of many people without leading to disease, if they break through into the bloodstream they can cause potentially fatal meningitis and septicemia. (Confocal image by Shao Jin Ong.) ...
12. CONFOCAL MICROSCOPY • Confocal microscopy can render
... The image is reconstructed serially, i.e. point by point, using a single photodetector, rather than in parallel (in bright field, phase contrast, etc.), where a 2D image is recorded via a detector array, such as a camera. The illumination light is focused down to a small spot at the sample plane ...
... The image is reconstructed serially, i.e. point by point, using a single photodetector, rather than in parallel (in bright field, phase contrast, etc.), where a 2D image is recorded via a detector array, such as a camera. The illumination light is focused down to a small spot at the sample plane ...
Confocal Microscopy
... Laser • Acronym: Light Amplification by Stimulated Emission of Radiation • Ordinary light emission: Comes from spontaneous decay of excited state to ground levels • Stimulated emission: molecule remains in excited state until stimulated to emit by incoming light that is insufficient to raise it to ...
... Laser • Acronym: Light Amplification by Stimulated Emission of Radiation • Ordinary light emission: Comes from spontaneous decay of excited state to ground levels • Stimulated emission: molecule remains in excited state until stimulated to emit by incoming light that is insufficient to raise it to ...
Laser confocal microscopy
... (DSTL), Porton Down, UK Technology Laser Confocal microscopy uses laser light to control the depth of field, and a pin hole to eliminate out of focus light, thus allowing visualisation of an image in a single horizontal plane. ...
... (DSTL), Porton Down, UK Technology Laser Confocal microscopy uses laser light to control the depth of field, and a pin hole to eliminate out of focus light, thus allowing visualisation of an image in a single horizontal plane. ...
Confocal Microscope - National Institute for Aviation Research
... Biomaterials in Orthopaedic Research (CIBOR) are available to both university researchers and industry clients. Confocal microscopy is a technique for obtaining high-resolution optical images with depth selectivity. The key features of a confocal are optical sectioning, and three-dimensional reconst ...
... Biomaterials in Orthopaedic Research (CIBOR) are available to both university researchers and industry clients. Confocal microscopy is a technique for obtaining high-resolution optical images with depth selectivity. The key features of a confocal are optical sectioning, and three-dimensional reconst ...
Confocal microscopy
Confocal microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. It enables the reconstruction of three-dimensional structures from the obtained images. This technique has gained popularity in the scientific and industrial communities and typical applications are in life sciences, semiconductor inspection and materials science.