Slide 1
... microscope is limited by diffraction of light. The image of an ideal point viewed through a circular aperture is blurred, and the diffracted image is known as an Airy disc. The size of the Airy disc depends on the wavelength of the laser source and the numerical aperture of the objective lens This A ...
... microscope is limited by diffraction of light. The image of an ideal point viewed through a circular aperture is blurred, and the diffracted image is known as an Airy disc. The size of the Airy disc depends on the wavelength of the laser source and the numerical aperture of the objective lens This A ...
Basics in Confocal Microscopy Handouts
... In Total Internal Reflection (TIRF) microscopy light is coupled into the optics above a critical angle which reflects the light totally but creates an evanescent wave about 50-200 nm next to the reflecting surface (cover slip). ...
... In Total Internal Reflection (TIRF) microscopy light is coupled into the optics above a critical angle which reflects the light totally but creates an evanescent wave about 50-200 nm next to the reflecting surface (cover slip). ...
Confocal Laser Scanning Microscope
... Depth coding (pseudo-color presentation of height information), Topographic view (3D reconstruction of the object’s topography), and 3D view. ...
... Depth coding (pseudo-color presentation of height information), Topographic view (3D reconstruction of the object’s topography), and 3D view. ...
Confocal microscopy
Confocal microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. It enables the reconstruction of three-dimensional structures from the obtained images. This technique has gained popularity in the scientific and industrial communities and typical applications are in life sciences, semiconductor inspection and materials science.