Scanning Tunneling Microscopy
... resolve would be directly proportional to the wavelength of the light shining on the object, and inversely proportional to the sine of the index of refraction of material from which the microscope’s lenses was made. Abbe’s limit implies that even the best optical microscope will be unable to distin ...
... resolve would be directly proportional to the wavelength of the light shining on the object, and inversely proportional to the sine of the index of refraction of material from which the microscope’s lenses was made. Abbe’s limit implies that even the best optical microscope will be unable to distin ...
High-speed addressable confocal microscopy for functional imaging
... sacrifice in spatial information.17,18 Unlike the line scan sysJournal of Biomedical Optics ...
... sacrifice in spatial information.17,18 Unlike the line scan sysJournal of Biomedical Optics ...
High Resolution Biomedical Imaging with Light and Sound
... illuminates tissue, where optical absorption and transient thermal expansion leads to ultrasound emission. Image contrast is based on the naturally occurring (endogenous) optical absorption in tissue. Spatial resolution and penetration depth are determined by the ultrasonic properties of tissue. Per ...
... illuminates tissue, where optical absorption and transient thermal expansion leads to ultrasound emission. Image contrast is based on the naturally occurring (endogenous) optical absorption in tissue. Spatial resolution and penetration depth are determined by the ultrasonic properties of tissue. Per ...
Aim: What instruments have aided in our knowledge of the cell?
... What do you know about viruses? They can make you very sick They can be transmitted from person to person There are different types of viruses ...
... What do you know about viruses? They can make you very sick They can be transmitted from person to person There are different types of viruses ...
Targeting delivery of chemotherapy agents by a cancer
... confluence were carefully washed and then incubated with the biotin-labeled TLS11a or control TD05 at a final concentration of 200 nM. After incubation at 4ºC for 30 min, cells were carefully washed before further incubation with a 1:200 dilution (optimized) of streptavidin-conjugated AlexaFluor 633 ...
... confluence were carefully washed and then incubated with the biotin-labeled TLS11a or control TD05 at a final concentration of 200 nM. After incubation at 4ºC for 30 min, cells were carefully washed before further incubation with a 1:200 dilution (optimized) of streptavidin-conjugated AlexaFluor 633 ...
Light Microscopy
... Alignment and Adjustment of the Light Microscope. Current Protocols in Cell Biology 4.1.1-4.1.26, John Wiley and Sons, N.Y. • Murphy, D. 2001. Fundamentals of Light Microscopy and Electronic Imaging. Wiley-Liss, N.Y. • Keller, H.E. 1995. Objective lenses for confocal microscopy. In “Handbook of bi ...
... Alignment and Adjustment of the Light Microscope. Current Protocols in Cell Biology 4.1.1-4.1.26, John Wiley and Sons, N.Y. • Murphy, D. 2001. Fundamentals of Light Microscopy and Electronic Imaging. Wiley-Liss, N.Y. • Keller, H.E. 1995. Objective lenses for confocal microscopy. In “Handbook of bi ...
the right microscope for the right sample
... epifluorescence microscopy only a thin slice of the sample is illuminated perpendicularly to the direction of observation. For illumination, a laser light-sheet is used. As only the actually observed section is illuminated, this method reduces the photodamage and stress induced on a living sample. A ...
... epifluorescence microscopy only a thin slice of the sample is illuminated perpendicularly to the direction of observation. For illumination, a laser light-sheet is used. As only the actually observed section is illuminated, this method reduces the photodamage and stress induced on a living sample. A ...
Optics in biology:
... Optics in biology: The use of optics in biology has evolved from the simple light microscope used by Darwin to the complex cryo-electron and live cell high resolution microscopes used today. With all these advances it can now be argued that we stand at the dawn of quantitative biology and optics pro ...
... Optics in biology: The use of optics in biology has evolved from the simple light microscope used by Darwin to the complex cryo-electron and live cell high resolution microscopes used today. With all these advances it can now be argued that we stand at the dawn of quantitative biology and optics pro ...
Biology 2014-2015 Ms. Mary Nugent Chapter One
... What are the levels of organization of living things? Describe each. What is the function of DNA How can biology enable you to make better decisions in your life? List the three types of microscopes. Write a brief paragraph explaining the advantages of using each of these. Which type of microscope w ...
... What are the levels of organization of living things? Describe each. What is the function of DNA How can biology enable you to make better decisions in your life? List the three types of microscopes. Write a brief paragraph explaining the advantages of using each of these. Which type of microscope w ...
Microscope History and Development
... The father of microscopy, Anton Van Leeuwenhoek of Holland (1632-1723). Anton Van Leeuwenhoek was the first to see and describe bacteria (1674), yeast plants, the teeming life in a drop of water, and the circulation of blood corpuscles in capillaries. ...
... The father of microscopy, Anton Van Leeuwenhoek of Holland (1632-1723). Anton Van Leeuwenhoek was the first to see and describe bacteria (1674), yeast plants, the teeming life in a drop of water, and the circulation of blood corpuscles in capillaries. ...
Microscopy and Immunoassays
... • Takes advantage of differences in density of transparent internal cell components • Uses a series of diaphragms to separate and recombine direct versus diffracted light rays ...
... • Takes advantage of differences in density of transparent internal cell components • Uses a series of diaphragms to separate and recombine direct versus diffracted light rays ...
Optics in Confocal Microscopy
... have several problems, however. If used with live cells under the coverslip in an aqueous (i.e. low refractive index) medium such as a physiological saline, the PSF deteriorates markedly with distance through the aqueous layer. Beyond 50 mm, the image is poorly resolved and the brightness falls, mak ...
... have several problems, however. If used with live cells under the coverslip in an aqueous (i.e. low refractive index) medium such as a physiological saline, the PSF deteriorates markedly with distance through the aqueous layer. Beyond 50 mm, the image is poorly resolved and the brightness falls, mak ...
Measurement of the 4Pi-confocal point spread function proves 75
... scanned in a plane containing the optical axis and the scattered light intensity was recorded as a function of the position. Scanning was performed by a scan stage that offered independently controlled x, y, and z axes (Photon Control, Cambridge, England). The bead was scanned 1.5 pm along the optic ...
... scanned in a plane containing the optical axis and the scattered light intensity was recorded as a function of the position. Scanning was performed by a scan stage that offered independently controlled x, y, and z axes (Photon Control, Cambridge, England). The bead was scanned 1.5 pm along the optic ...
microscopy
... The Cell Theory • All living organisms are made of one or more cells. • The cell is the basic organizational unit of life. • All cells come from previously existing cells. ...
... The Cell Theory • All living organisms are made of one or more cells. • The cell is the basic organizational unit of life. • All cells come from previously existing cells. ...
0.61 x wavelength of light
... Biological microscopy problem: Cells are 3D objects, and pictures are 2D images. •Single cells are thicker than the wavelength of visible light, so they must be visualized with many “optical sections” •In an image of one section, one must remove light from other sections •Achieving a narrow “depth- ...
... Biological microscopy problem: Cells are 3D objects, and pictures are 2D images. •Single cells are thicker than the wavelength of visible light, so they must be visualized with many “optical sections” •In an image of one section, one must remove light from other sections •Achieving a narrow “depth- ...
Visualizing Prokaryote Cells
... irregular and occures along lines of weakness like the plasma membrane or surfaces of organelles. 3. Surface ice is removed by a vacuum (freeze etching) 4. A thin layer of carbon is evaporated vertically onto the surface to produce a carbon replica. 5. Surface is shadowed with a platinum vapor. 6. O ...
... irregular and occures along lines of weakness like the plasma membrane or surfaces of organelles. 3. Surface ice is removed by a vacuum (freeze etching) 4. A thin layer of carbon is evaporated vertically onto the surface to produce a carbon replica. 5. Surface is shadowed with a platinum vapor. 6. O ...
Lecture 19 - Home - Engineering
... excitation emission wavelengths to look at different cell components • Cell nucleus stained with blue Hoechst dye • Mitochondria stained with Mitotracker red • Actin cytoskeleton stained with phalloidin derivative conjugated to Alexa 488 (green) ...
... excitation emission wavelengths to look at different cell components • Cell nucleus stained with blue Hoechst dye • Mitochondria stained with Mitotracker red • Actin cytoskeleton stained with phalloidin derivative conjugated to Alexa 488 (green) ...
Confocal microscopy with a volume holographic filter
... The pinhole preceding the detector in a confocal microscope is a shift-variant optical element. On-axis in-focus point-source objects are imaged exactly inside the pinhole and give maximal intensity. An out-offocus object, even when it is on axis, is equivalent to an extended source on the input foc ...
... The pinhole preceding the detector in a confocal microscope is a shift-variant optical element. On-axis in-focus point-source objects are imaged exactly inside the pinhole and give maximal intensity. An out-offocus object, even when it is on axis, is equivalent to an extended source on the input foc ...
Fluorescence, confocal microscopy
... Quenching and photobleaching reduce the amount of fluorescence and are of great practical significance to the microscopist. Quenching reduces the quantum yield of a fluorochrome without changing its fluorescence emission spectrum and is caused by interactions with other molecules including other flu ...
... Quenching and photobleaching reduce the amount of fluorescence and are of great practical significance to the microscopist. Quenching reduces the quantum yield of a fluorochrome without changing its fluorescence emission spectrum and is caused by interactions with other molecules including other flu ...
Confocal microscopy
Confocal microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. It enables the reconstruction of three-dimensional structures from the obtained images. This technique has gained popularity in the scientific and industrial communities and typical applications are in life sciences, semiconductor inspection and materials science.