LEGENDS OF SUPPORTING INFORMATION Supplemental figure
... was its final destination. Upon wortmannin treatment of GOLD36::GOLD36-mRFP cotyledons, we found that GOLD36-mRFP was partially secreted to the apoplastic space, confirming our hypothesis that GOLD36-mRFP is destined to post-ER compartments, specifically the vacuole. As shown in this figure, confoca ...
... was its final destination. Upon wortmannin treatment of GOLD36::GOLD36-mRFP cotyledons, we found that GOLD36-mRFP was partially secreted to the apoplastic space, confirming our hypothesis that GOLD36-mRFP is destined to post-ER compartments, specifically the vacuole. As shown in this figure, confoca ...
Forensic Science
... •The examiner studying a specimen under a microscope can simultaneously obtain the visible absorption spectrum or IR spectrum of the material being observed. •This instrument is especially useful in the examination of trace evidence, paint, fiber, and ink evidence. The Scanning Electron Microscope ...
... •The examiner studying a specimen under a microscope can simultaneously obtain the visible absorption spectrum or IR spectrum of the material being observed. •This instrument is especially useful in the examination of trace evidence, paint, fiber, and ink evidence. The Scanning Electron Microscope ...
Optical Properties of Colloids
... microscope (resolution limit of c. 5 nm) is, in general, less than that in a transmission electron microscope, but the major advantage of the technique (which is a consequence of the low numerical aperture) is the great depth of focus which can be achieved. At magnifications in the range of optica ...
... microscope (resolution limit of c. 5 nm) is, in general, less than that in a transmission electron microscope, but the major advantage of the technique (which is a consequence of the low numerical aperture) is the great depth of focus which can be achieved. At magnifications in the range of optica ...
Microscopy
... In the third lab, we learned about the basic configuration of the compound microscope. In general, an object you wish to image is 3-dimensional. Although you may wish to image one plane of a specimen, out-of-plane light will cause distortion of your image and make it look blurry. The confocal micros ...
... In the third lab, we learned about the basic configuration of the compound microscope. In general, an object you wish to image is 3-dimensional. Although you may wish to image one plane of a specimen, out-of-plane light will cause distortion of your image and make it look blurry. The confocal micros ...
ch_04 - studylib.net
... background. Confocal Microscopes Confocal microscopes use fluorescent dyes in conjunction with ultraviolet lasers to illuminate the fluorescent chemicals in only one thin plane of a specimen at a time. Several images are taken and digitized, and then computers construct three-dimensional images of t ...
... background. Confocal Microscopes Confocal microscopes use fluorescent dyes in conjunction with ultraviolet lasers to illuminate the fluorescent chemicals in only one thin plane of a specimen at a time. Several images are taken and digitized, and then computers construct three-dimensional images of t ...
Microscopes and Cells
... • All living things are composed of cells • All cells come from preexisting cells by cell division • The cell theory applies to all organisms – Single cell organisms – Multicellular organisms (many start life as a single cell) ...
... • All living things are composed of cells • All cells come from preexisting cells by cell division • The cell theory applies to all organisms – Single cell organisms – Multicellular organisms (many start life as a single cell) ...
Culturing A. castellanii - College of Science
... and confocal laser scanning microscopy (CSLM) to study both the free living metabolically active trophozoite form and dormant cyst form. We imaged fixed and dehydrated trophozoites and cysts with SEM. Trophozoites were imaged with CSLM in transmitted light and using fluorescent emissions from GFP. C ...
... and confocal laser scanning microscopy (CSLM) to study both the free living metabolically active trophozoite form and dormant cyst form. We imaged fixed and dehydrated trophozoites and cysts with SEM. Trophozoites were imaged with CSLM in transmitted light and using fluorescent emissions from GFP. C ...
Optical and Electron Microscopy
... There exist two main families of electron microscope. The transmission electron microscope (TEM) is an instrument that achieves the highest resolution. (The terms TEM and HREM are to some extent interchangeable, although the TEM is not used exclusively for high-resolution observations). The TEM, as ...
... There exist two main families of electron microscope. The transmission electron microscope (TEM) is an instrument that achieves the highest resolution. (The terms TEM and HREM are to some extent interchangeable, although the TEM is not used exclusively for high-resolution observations). The TEM, as ...
Preview Sample 2
... Tools of the Laboratory: The Methods for Studying Microorganisms In culturing microorganisms, many can be cultured on artificial media, but some can be cultured only in living tissue or in cells such as with viruses. Artificial media are classified by their physical state as either liquid, semisolid ...
... Tools of the Laboratory: The Methods for Studying Microorganisms In culturing microorganisms, many can be cultured on artificial media, but some can be cultured only in living tissue or in cells such as with viruses. Artificial media are classified by their physical state as either liquid, semisolid ...
5 The Basics of Confocal Microscopy
... microscopy has become an invaluable tool for imaging thin optical sections in living and fixed specimens ranging in thickness up to 100 micrometers. In fact the confocal microscope is often capable of revealing the presence of single molecule (Peterman, Sosa et al., 2004). Modern confocal microscope ...
... microscopy has become an invaluable tool for imaging thin optical sections in living and fixed specimens ranging in thickness up to 100 micrometers. In fact the confocal microscope is often capable of revealing the presence of single molecule (Peterman, Sosa et al., 2004). Modern confocal microscope ...
Compiled questions (docx 32 kB)
... scanning microscope the dependence is linear. Nonlinear microscopy methods might have linear or quadratic dependence. Which dependence on concentration you would expect in following nonlinear methods: (a) SHG, (b) SFM, (c) TPFE, (d) CARS, (e) heterodyne CARS, (f) SRS. 2. In all microscopy methods wh ...
... scanning microscope the dependence is linear. Nonlinear microscopy methods might have linear or quadratic dependence. Which dependence on concentration you would expect in following nonlinear methods: (a) SHG, (b) SFM, (c) TPFE, (d) CARS, (e) heterodyne CARS, (f) SRS. 2. In all microscopy methods wh ...
S10 Notes- The Technology of Cell Research
... Types of microscopes: Microscopes differ in what they use for energy to view small things and what sizes of objects we can see clearly with them. Highlight the energy source used in each microscope below. 1. Simple microscope: a microscope composed of only one lens (magnifying glass). Specimen is ...
... Types of microscopes: Microscopes differ in what they use for energy to view small things and what sizes of objects we can see clearly with them. Highlight the energy source used in each microscope below. 1. Simple microscope: a microscope composed of only one lens (magnifying glass). Specimen is ...
Precision High Numerical Aperture Scanning System for
... University of Rochester Rochester, New York ...
... University of Rochester Rochester, New York ...
3D widefield light microscopy with better than 100 nm axial resolution
... and a Photometrics (Tucson, AZ) cooled CCD camera. The excitation light was s-polarised using a crude tangential polariser (Oriel, Stratford, CT). Superposition of the two image beams was maintained by controlling the XYZ position of one of the objective lenses, using electrostrictive actuators oper ...
... and a Photometrics (Tucson, AZ) cooled CCD camera. The excitation light was s-polarised using a crude tangential polariser (Oriel, Stratford, CT). Superposition of the two image beams was maintained by controlling the XYZ position of one of the objective lenses, using electrostrictive actuators oper ...
Photo Contest Winners Member Lens:
... Alexander R. Albrecht University of New Mexico, U.S.A. Laser beams of three primary colors are guided inside streams of liquid into a ceramic bowl, where they combine to form white light. “This image beautifully demonstrates total internal reflection and three-color mixing, creating a visceral, inte ...
... Alexander R. Albrecht University of New Mexico, U.S.A. Laser beams of three primary colors are guided inside streams of liquid into a ceramic bowl, where they combine to form white light. “This image beautifully demonstrates total internal reflection and three-color mixing, creating a visceral, inte ...
Title: Unraveling the association between genetic integrity and
... power of the wide field images light (or fluorescent) microscopy, thereby facilitating rapid identification of specific cellular structures and ultrastructural changes. CLEM has been used in the identification and localization of cellular and subcellular events. (de Boer et al., Nature methods 2015 ...
... power of the wide field images light (or fluorescent) microscopy, thereby facilitating rapid identification of specific cellular structures and ultrastructural changes. CLEM has been used in the identification and localization of cellular and subcellular events. (de Boer et al., Nature methods 2015 ...
Title: Deconvolution fluorescence microscopy of yeast cells Author
... Abstract: Fluorescence microscopy presents an fast and cheap alternative to more advanced imaging methods like confocal and electron microscopy, even though it is subject to heavy image distortion. It is possible to recover most of the original distortion-free image using deconvolution in computer i ...
... Abstract: Fluorescence microscopy presents an fast and cheap alternative to more advanced imaging methods like confocal and electron microscopy, even though it is subject to heavy image distortion. It is possible to recover most of the original distortion-free image using deconvolution in computer i ...
Document
... Then, an input photon with a particular wavelength strikes an excited atom of the medium and pushes it back to a lower energy state to emit a photon with the same wavelength and in phase and in the same direction as the input photon. The process is called stimulated emission. ...
... Then, an input photon with a particular wavelength strikes an excited atom of the medium and pushes it back to a lower energy state to emit a photon with the same wavelength and in phase and in the same direction as the input photon. The process is called stimulated emission. ...
Diffracted Light Contrast: Improving the Resolution - Microscopy-UK
... unsuccessful. The difficulty may not be one of resolution because Figs. 2a and 3F would certainly suggest DLC is capable of generating sufficient resolution. Instead, it would seem that contrast may be the limiting factor. DLC may circumvent the Abbé limit for two reasons. First, because the effecti ...
... unsuccessful. The difficulty may not be one of resolution because Figs. 2a and 3F would certainly suggest DLC is capable of generating sufficient resolution. Instead, it would seem that contrast may be the limiting factor. DLC may circumvent the Abbé limit for two reasons. First, because the effecti ...
Interpreting Fluorescence Microscopy Images and Measurements.
... scanning confocal microscopy (also called laser scanning confocal) uses a focused light source to scan the image point by point and a pinhole at the image plane to block out-of-focus fluorescence from reaching the detector. Multipoint or slit scanning confocals (such as the spinning disk confocal) u ...
... scanning confocal microscopy (also called laser scanning confocal) uses a focused light source to scan the image point by point and a pinhole at the image plane to block out-of-focus fluorescence from reaching the detector. Multipoint or slit scanning confocals (such as the spinning disk confocal) u ...
Multiphoton Microscopy: Turnkey Femtosecond - Spectra
... repetition rate) together with the high-NA (numerical aperture) microscope objective generate photon densities within the focal volume that are enough to drive two- (and even three-) photon absorption. Elsewhere in the sample, the power density is too low for nonlinear excitation, so there is an inh ...
... repetition rate) together with the high-NA (numerical aperture) microscope objective generate photon densities within the focal volume that are enough to drive two- (and even three-) photon absorption. Elsewhere in the sample, the power density is too low for nonlinear excitation, so there is an inh ...
A typical animal cell The diagram below shows the typical structure
... Chromatin contains DNA, the inherited material which controls the various activities inside the cell. ...
... Chromatin contains DNA, the inherited material which controls the various activities inside the cell. ...
Confocal microscopy
Confocal microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. It enables the reconstruction of three-dimensional structures from the obtained images. This technique has gained popularity in the scientific and industrial communities and typical applications are in life sciences, semiconductor inspection and materials science.