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Physics 422 - Spring 2015 - Assignment #5
... 3. (a) Calculate the distance to the object focal point, fo , and the image focal point fi for a single spherical concave refracting surface with radius of curvature R = −10 cm, made of a material with index of refraction n2 = 1.5, and with air (n1 = 1) on the object side. (b) Calculate fo and fi f ...
... 3. (a) Calculate the distance to the object focal point, fo , and the image focal point fi for a single spherical concave refracting surface with radius of curvature R = −10 cm, made of a material with index of refraction n2 = 1.5, and with air (n1 = 1) on the object side. (b) Calculate fo and fi f ...
Lec.1
... a prism that generates two distinct beams; these beams pass through the specimen and enter the objective lens where they are recombined into a single beam. Because of slight differences in refractive index of the substances each beam passed through, the combined beams are not totally in phase but in ...
... a prism that generates two distinct beams; these beams pass through the specimen and enter the objective lens where they are recombined into a single beam. Because of slight differences in refractive index of the substances each beam passed through, the combined beams are not totally in phase but in ...
Center for electron nanoscopy, DTU
... the construction of a purpose-built building. The microscopes are very special: 2 Titans, both Cs corrected, with monochromators and full analytical capabilities are to achieve spatial resolutions of 0.7Å and spectroscopy resolutions of 0.1eV. One of the Titans is to be equipped with an environmenta ...
... the construction of a purpose-built building. The microscopes are very special: 2 Titans, both Cs corrected, with monochromators and full analytical capabilities are to achieve spatial resolutions of 0.7Å and spectroscopy resolutions of 0.1eV. One of the Titans is to be equipped with an environmenta ...
“Methods in Histology” Major types of Light Microscopy Microscopy
... beam) to scan the pre-coated specimen surface. • As the probe scans across the specimen, byproducts of secondary electrons, backscatter electrons, x-rays, & photons are produced. • Secondary electrons are low energy electrons (< 50 ev) emitted from the surface of the specimen (up to a depth of 20 Å) ...
... beam) to scan the pre-coated specimen surface. • As the probe scans across the specimen, byproducts of secondary electrons, backscatter electrons, x-rays, & photons are produced. • Secondary electrons are low energy electrons (< 50 ev) emitted from the surface of the specimen (up to a depth of 20 Å) ...
Molecular Cell Biology 6/e
... illuminated. This SEM use electron illumination. The image A dissection microscope is light This microscope uses a laser light. This gives a 2-D view. is seen in 3-D. It has high magnification illuminated. The image that appears is light is used because of the wavelength. Thin slices of and high res ...
... illuminated. This SEM use electron illumination. The image A dissection microscope is light This microscope uses a laser light. This gives a 2-D view. is seen in 3-D. It has high magnification illuminated. The image that appears is light is used because of the wavelength. Thin slices of and high res ...
bright field microscopy
... When to use Fluorescence microscopy •Used to study specimens, which can be made to fluoresce. •Certain material emits energy detectable as visible light when irradiated with the light of a specific wavelength. The sample can either be fluorescing in its natural form like chlorophyll and some mineral ...
... When to use Fluorescence microscopy •Used to study specimens, which can be made to fluoresce. •Certain material emits energy detectable as visible light when irradiated with the light of a specific wavelength. The sample can either be fluorescing in its natural form like chlorophyll and some mineral ...
Microscope renaissance
... cells. His equipment was able to pick up a flash of fluorescent Venus yellow every time the cell made a single molecule of a protein, allowing biologists to more precisely study how cells create proteins -- some of which are involved in diseases. The one weakness of both the E. coli movie and the br ...
... cells. His equipment was able to pick up a flash of fluorescent Venus yellow every time the cell made a single molecule of a protein, allowing biologists to more precisely study how cells create proteins -- some of which are involved in diseases. The one weakness of both the E. coli movie and the br ...
MPRI talk - NECSA - Indico
... - dye laser range 630 – 660 nm with DCM dye - double-grating scanning spectrometer 350 – 900 nm - 15W argon ion laser - cooled photon counter and photon counting electronics ...
... - dye laser range 630 – 660 nm with DCM dye - double-grating scanning spectrometer 350 – 900 nm - 15W argon ion laser - cooled photon counter and photon counting electronics ...
Imaging of vascular gene expression is an important part of
... distribution and localization of transgene delivery at the target vessel wall; (b) level of transgene expression for efficient therapeutic effect on the targets; and (c) the functional period of therapeutic genes at the targets. Optical imaging, which is based on the detection of fluorescence or emi ...
... distribution and localization of transgene delivery at the target vessel wall; (b) level of transgene expression for efficient therapeutic effect on the targets; and (c) the functional period of therapeutic genes at the targets. Optical imaging, which is based on the detection of fluorescence or emi ...
Living Things
... • Until the late 1600s, no one knew cells existed because there was no way to see them. • One square centimeter of your skin’s surface contains more than 100,000 cells. ...
... • Until the late 1600s, no one knew cells existed because there was no way to see them. • One square centimeter of your skin’s surface contains more than 100,000 cells. ...
“Put that in the Form of a Question, Please!”
... microscope contains the magnifying lens you look ...
... microscope contains the magnifying lens you look ...
Fundamental Limits in Confocal Microscopy
... matters, such calculations will be immensely complex and therefore take time: time seldom available when viewing living cells. In general, it seems that we must accustom ourselves to more modest performance when viewing living specimens in a 3D light microscope. Given the distortion noted above, it ...
... matters, such calculations will be immensely complex and therefore take time: time seldom available when viewing living cells. In general, it seems that we must accustom ourselves to more modest performance when viewing living specimens in a 3D light microscope. Given the distortion noted above, it ...
Birla Institute of Technology and Science, Pilani and Elite School of Optometry
... 1.5 Electromagnetic spectrum; visible spectrum; UV; UV-A, UV-B, UV-C; IR - far and near IR radiations; X-rays ...
... 1.5 Electromagnetic spectrum; visible spectrum; UV; UV-A, UV-B, UV-C; IR - far and near IR radiations; X-rays ...
Study Guide Quiz #2
... 3. Resolution: page 15. It is the ability to distinguish adjacent objects. It enhances the clarity of an image. The resolution of unaided eye is low. It is higher in case of compound microscope but highest in an electron microscope. 4. Parfocal: Compound microscope is in focus at low power magnifica ...
... 3. Resolution: page 15. It is the ability to distinguish adjacent objects. It enhances the clarity of an image. The resolution of unaided eye is low. It is higher in case of compound microscope but highest in an electron microscope. 4. Parfocal: Compound microscope is in focus at low power magnifica ...
Microbiology 155
... • Operate like phase contrast microscopes but with much greater resolution • This produces almost a three dimensional image ...
... • Operate like phase contrast microscopes but with much greater resolution • This produces almost a three dimensional image ...
Microbiology: A Systems Approach, 2nd ed.
... condenser using an adjustable iris diaphragm or using special dyes help increase resolution at higher magnifications ...
... condenser using an adjustable iris diaphragm or using special dyes help increase resolution at higher magnifications ...
Developmental Biology Brochure
... Spectral imaging: With only one scan required to capture all spectral data in a sample, image acquisition time is reduced. Simultaneous excitation by up to four lasers is possible with fast spectral unmixing (512 x 512 pixel, 32channel unmixed in less than 1 second). Multiphoton: Ideal for imaging d ...
... Spectral imaging: With only one scan required to capture all spectral data in a sample, image acquisition time is reduced. Simultaneous excitation by up to four lasers is possible with fast spectral unmixing (512 x 512 pixel, 32channel unmixed in less than 1 second). Multiphoton: Ideal for imaging d ...
READY TO TRAVEL INSIDE A LIVING CELL AS NEVER BEFORE
... The Nobel Prize 2014 for chemistry was awarded to S. Hell, E. Betzig, and W. Moerner, who did not believe in the presumed limitations of light and made revolutionary discoveries in the field of fluorescence microscopy. Dr. Yann Cotte, CEO and founder of Nanolive, shared with them the same skepticism ...
... The Nobel Prize 2014 for chemistry was awarded to S. Hell, E. Betzig, and W. Moerner, who did not believe in the presumed limitations of light and made revolutionary discoveries in the field of fluorescence microscopy. Dr. Yann Cotte, CEO and founder of Nanolive, shared with them the same skepticism ...
Two-Photon Excited Fluorescence Microscopy - Spectra
... generated everywhere along the optical axis. Upon twophoton absorption at 800 nm, fluorescence is observed exclusively at the focal point of the objective. By using high numerical aperture objectives, fluorescence induced by twophoton absorption can be confined to only a few hundred nanometers of pa ...
... generated everywhere along the optical axis. Upon twophoton absorption at 800 nm, fluorescence is observed exclusively at the focal point of the objective. By using high numerical aperture objectives, fluorescence induced by twophoton absorption can be confined to only a few hundred nanometers of pa ...
optical microscope inverted optical microscope fluorescence
... Optical microscope is the oldest and most comman microscope. It enlarges the images 2000 times and works in visible light. Inverted microscope has the same properties with optical microscope but enlarges the images only 60 times.Its very important property is working with alive organism much longer ...
... Optical microscope is the oldest and most comman microscope. It enlarges the images 2000 times and works in visible light. Inverted microscope has the same properties with optical microscope but enlarges the images only 60 times.Its very important property is working with alive organism much longer ...
report - CREATE project
... autocorrelation function with nonlinear crystal placed at the microscope output (Fig. 2a). The first imaging test was performed with a piece of paper. We verified that paper absorbs 745 nm through two photon absorption and emits visible light.In order to compare functions of the signal intensity ver ...
... autocorrelation function with nonlinear crystal placed at the microscope output (Fig. 2a). The first imaging test was performed with a piece of paper. We verified that paper absorbs 745 nm through two photon absorption and emits visible light.In order to compare functions of the signal intensity ver ...
Lecture 1 (01/21/14) - Course introduction and Basic Microscopy
... Lipid bilayers, for example, produce excellent contrast in DIC because of the difference in refractive index between aqueous and lipid phases of the cell. In addition, cell boundaries in relatively flat adherent mammalian and plant cells, including the plasma membrane, nucleus, vacuoles, mitochondri ...
... Lipid bilayers, for example, produce excellent contrast in DIC because of the difference in refractive index between aqueous and lipid phases of the cell. In addition, cell boundaries in relatively flat adherent mammalian and plant cells, including the plasma membrane, nucleus, vacuoles, mitochondri ...
Light sheet-based fluorescence microscopy: more dimensions, more
... a regular fluorescence wide-field microscope. When operating at a numerical aperture (NA) below 0.6, the axial resolution is dominated by the thickness of the light sheet and is less affected by the properties of the detection lens. The situation is, thus, different than in an epifluorescence setup. ...
... a regular fluorescence wide-field microscope. When operating at a numerical aperture (NA) below 0.6, the axial resolution is dominated by the thickness of the light sheet and is less affected by the properties of the detection lens. The situation is, thus, different than in an epifluorescence setup. ...
Slide 1
... (a) Distribution of detected photons from a single source – standard deviation s (b) Crosses are mean position estimated by fitting a Gaussian to (a). Error in the mean should be s/N, where N photons are detected. Each cross is the mean position estimated from a separate activation cycle of the flu ...
... (a) Distribution of detected photons from a single source – standard deviation s (b) Crosses are mean position estimated by fitting a Gaussian to (a). Error in the mean should be s/N, where N photons are detected. Each cross is the mean position estimated from a separate activation cycle of the flu ...
Confocal microscopy
Confocal microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. It enables the reconstruction of three-dimensional structures from the obtained images. This technique has gained popularity in the scientific and industrial communities and typical applications are in life sciences, semiconductor inspection and materials science.