Download Imaging Services

and second harmonic generation microscopy in high
containment environment. In addition, specialized imaging
techniques such as 3D reconstructions, colocalization
analysis, Flourescence Resonance Energy Transfer
(FRET), time-lapsed microscopy, Fluorescence Recovery
after Photobleaching (FRAP) and fluorescent protein
photoactivation techniques can be performed within the
cores to monitor dynamic processes such as cell trafficking
and cell-cell interactions in cells and in vivo in animal
models.
Advanced imaging instrumentation available
for cellular imaging within the GNL include:
1. BSL-2 Confocal and multiphoton microscopy:
Olympus Fluoview 1000MPE system configured with
an upright BX61 microscope. This system is configured
to optimally work with thick specimens but also can be
used for high resolution imaging of cellular preparations.
2. BSL-3 Confocal and multiphoton microscope:
Olympus Fluoview 1000 system configured with an
inverted IX81 microscope. This system is configured to
optimally work with cell culture preparations including
live cell imaging in single and multiwell substrates, but
also tissue can be fitted.
3. Image analysis and processing: Resources include
a full license for Imaris (Bitplane) and multiple
licenses for Metamorph Premier (Molecular devices).
Both of these image processing and reconstruction
packages can be used for quantitative image analysis of
multidimensional images including live cell time lapse
studies.
Whole Body Animal Imaging Cores
Availability of the new imaging technology for small animal
studies has provided researchers with
capabilities to perform in vivo studies
in animal models with resolution and
sensitivity that were only possible under
in vitro conditions just a few years ago.
The newly developed and commercialized
imaging instruments can provide highly
sensitive capabilities for dynamic
imaging of targeted tissue with high
spatial resolution enabling investigators
to monitor various changes in molecular
and morphological features of infected
tissue non-invasively as function of time and therapeutic
interventions.
The GNL whole body imaging capabilities include:
1) optical imaging system (IVIS Spectrum) which can be
used to visualize molecular
responses of tissues or organs to
biological agents non-invasively
over long periods of time through
the use of in vivo fluorescent and
bioluminescence reporters and
2) computed tomography (CT)
and positron emission tomography
(PET) that allow for whole body
structural and molecular imaging
in large animal models to evaluate
the response of in vivo systems to
biological agents and candidate therapies.
For additional information, please contact the Imaging Service Division by emailing Dr. Massoud Motamedi ([email protected]),
Leoncio Vergara ([email protected]), Juan Olano ([email protected]), or Gracie Vargas ([email protected]).