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content - Theses
content - Theses

... 4.5 of the mATPase reaction are classified as type 1 fibres, while the fibres that are stained positively after the alkaline preincubation at pH 10.3 and remain unstained after both acid preincubations at pH 4.3 and 4.5 are type 2A fibres and the fibres characterised by high mATPase activity after ...
Hormonal correlates of changes in interest in unrelated infants
Hormonal correlates of changes in interest in unrelated infants

... In past research on human and nonhuman primates, maternal responsiveness and behavior has been thought of as an experiential, cognitive mechanism; however, recent findings have shown that maternal motivation and behavior may not be entirely divorced from the endocrine system. To investigate the rela ...
Effects of Acute Exposure to PCBs 126 and 153 on Anterior Pituitary
Effects of Acute Exposure to PCBs 126 and 153 on Anterior Pituitary

... increasing the duration of the anesthesia could decrease hormone levels (Schultz et al., 1995; Strutton and Coen, 1996), and, in contrast to sodium pentobarbital, isoflurane was found to induce a more rapid anesthesia. This occurred within a similar time frame between rats, following initiation of t ...
ANABOLIC-ANDROGENIC STEROIDS
ANABOLIC-ANDROGENIC STEROIDS

... severe muscle loss ...
Behavioral flexibility is increased by optogenetic inhibition of
Behavioral flexibility is increased by optogenetic inhibition of

... by inhibition during feedback We investigated the effect on behavioral flexibility of optogenetic inhibition during specific time segments of a task that involved within-session switching of contingencies. Rats (n ¼ 8) were trained to criterion on tasks of increasing difficulty, initially learning t ...
Food Restriction Differentially Affects mRNAs Encoding the Major
Food Restriction Differentially Affects mRNAs Encoding the Major

... These data indicate that anti-aging effects begin shortly after the onset of FR and are cumulative. This concept is important theoretically as well as practically, since it indicates that insight into the anti-aging mechanisms induced by FR may be obtained by studying young FR animals. Although shor ...
Gradual increase in neuronal density of rats
Gradual increase in neuronal density of rats

... the LGN. The figures on the right are x 20 magnification of the adjacent figures on the left. ...
LXXXI. HORMONES OF THE ANTERIOR PITUITARY LOBE.
LXXXI. HORMONES OF THE ANTERIOR PITUITARY LOBE.

... up with sterile sand in a sterile mortar and a little water was added to make a paste, then 100 cc. of 0x1 N sodium hydroxide were added and the mixture was well shaken. After standing in the ice-chest for 18?hours the mixture was carefully neutralised to phenol red by addition of 0.1 N hydrochloric ...
The interplay between oxidative stress and brain-derived
The interplay between oxidative stress and brain-derived

... 1 dinitrophenylhydrazine (DNPH) for 15 min, followed by neutralization with a solution containing glycerol and b-mercaptoethanol. These samples were electrophoresed on an 8% polyacrylamide gel and electrotransferred to a nitrocellulose membrane. After blocking, membranes were incubated overnight wi ...
Calcium Binding Protein-Like lmmunoreactivity Labels the Terminal
Calcium Binding Protein-Like lmmunoreactivity Labels the Terminal

... Optiphot). Specifically, we measured the amount of time required for a constant level of exposure of photographic film having a given sensitivity (we chose ASA 12) at different spots within an immunoreactive zone in the ICc. The metering spot is designed to cover 1% of the area in view at any given ...
Neuro Objectives 22 - U
Neuro Objectives 22 - U

... Medial lemniscus (Contralateral touch/position): medial in caudal medulla → medial to inferior olivary nucleus in the rostral medulla → dorsolateral in the basal pons → ventromedial to the spinothalamic tract in the midbrain Spinothalamic tract (Contralateral pain/temperature): lateral in caudal med ...
Endocrine Effects of Marijuana
Endocrine Effects of Marijuana

... which lasted 1 hour.9 Chronic effects of cannabinoid administration are less clear. Although dose-dependent decreases in LH have been observed with chronic administration of ∆9- THC,10 the effect of chronic exposure is less dramatic than that of acute administration7 and may be related to the develo ...
Distribution of GABA‐like immunoreactivity in the rat amygdaloid
Distribution of GABA‐like immunoreactivity in the rat amygdaloid

... anatomical basis of these opposite activities has not been et al., '65; Leonard and Scott, '71; De Olmos, '72; Krettek fully elucidated. and Price, '78b; Aggleton et al., '80; Post and Mai, '80; The inhibitory trasmitter a-aminobutyric acid (GABA) Mehler, '80; Nitecka, '81; Price, '81b). This raises ...
08. pons + midbrain
08. pons + midbrain

...  A unilateral brain stem lesion : caused by stroke,tumour or multiple sclerosis causes : 1-epsilateral cranial nerve dysfunction + contralateral spastic hemiparesis. 2-hyperreflexia & an extensor plantar response (upper motor neurone lesion). 3-contalateral hemisensory loss. 4-ipsilateral incoordin ...
transgene expression using adenoviral vectors: impact of promoters
transgene expression using adenoviral vectors: impact of promoters

... to date, many studies have employed “constitutive” promoters such as human cytomegalovirus (HCMV), human synapsin-1 (Syn1), tubulin-␣1 (T␣1), and neuron-specific enolase (NSE) for transgene expression in brain nuclei. It is known that such promoters may discriminate between neurons and glia; for exa ...
Neuropeptidergic Organization of the Suprachiasmatic Nucleus in
Neuropeptidergic Organization of the Suprachiasmatic Nucleus in

... advances or delays depending on circadian phase. The phase response curve for the phase shifting effects of NPY are different from that caused by light [2,33,60,64]. In addition, the level of NPY in the SCN shows two peaks at the day/night and night/day transition periods [7,37,68,71], which suggest ...
Effect of Chlorpyrifos on Thyroid Gland of Adult Male
Effect of Chlorpyrifos on Thyroid Gland of Adult Male

... arrows). Others have shrunken nuclei (thin arrows). Notice: scanty lightly stained colloid (C) and thick collagen fibers between follicles (S). Touilidine blue X 1000 ...
Tyrosine Hydroxylase in the Rat Parabrachial Region: Ultrastructural
Tyrosine Hydroxylase in the Rat Parabrachial Region: Ultrastructural

... of HRP according to the TMB procedure described above. The sections were then rinsed in 0.01 M acetate buffer followed bv a brief 5 set rinse in 0.1 M phosphate buffer. The reaction product was stabilized by a 4 min incubation in a solution of 0.05% DAB, 0.02% cobalt acetate, and 0.0 1% H,O, in 0.1 ...
Size and Shape of the Pituitary Gland with MR Imaging from
Size and Shape of the Pituitary Gland with MR Imaging from

... and the boys no larger than 7mm, this is in agreement with values provided by Elster et al.[2]. In some age groups, the statistically significant conclusion as the subject was less number. Muhammad Faisal Ikram et al.[7], recent study, determined all subject 220 cases and range age was the same as t ...
Experimentally cross-wired lingual taste nerves can restore normal
Experimentally cross-wired lingual taste nerves can restore normal

... taste receptor cells or the AT taste receptor cells. In addition, one surgery, designed to prevent the reinnervation of both taste receptor cell fields, was performed. Two sham-surgical groups (one stimulated with quinine, the other stimulated with water) were also included, for a total of seven gro ...
Cannabis Use and the Adolescent Brain
Cannabis Use and the Adolescent Brain

... adolescents who had used cannabis by age 15 were found to be 6 times more likely to experience psychotic symptoms at age 26 than adolescents who had not (after controlling for psychotic symptoms at age 11). This risk is even higher for adolescents who are already experiencing some sub-clinical sympt ...
Caudo‐rostral brain spreading of α‐synuclein through vagal
Caudo‐rostral brain spreading of α‐synuclein through vagal

... An experimental strategy was designed by which human a‐syn (ha‐syn) could be specifically overexpressed in the rat MO and then “traced forward” to assess its potential spreading. Recombinant adeno‐associated viral vectors (AAV) expressing either wild‐type ha‐syn or green fluorescent protein (GFP) were ...
Transgenic Mice Expressing Green Fluorescent Protein under the
Transgenic Mice Expressing Green Fluorescent Protein under the

... S-labeled cRNA, the plasmids were linearized by digestion with SalI and subjected to in vitro transcription with T7 RNA polymerase according to the manufacturer’s protocol (Ambion, Austin, TX). The 35Slabeled cRNA probe for the MC4-R mRNA was then diluted to 10 6 cpm/ml in a hybridization solution c ...
Mechanism of Nitrofen Teratogenesis
Mechanism of Nitrofen Teratogenesis

... in maternal tissues, while the 4'-amino and 4'-acetylamine derivatives were found at lower levels and all exhibited single phase kinetics (data not shown). The parent compound alone was found in the embryo from 1.5 to 72 hr after maternal exposure (limit of detection 0.03 ,ug/injection), and levels ...
Effects of Acute and Neurotoxic Exposure in the Rat
Effects of Acute and Neurotoxic Exposure in the Rat

... in the session was similar regardless of delay value. This is not the case for rats exposed to MDMA, which emitted the most responses when reinforcement was immediate. This effect is most evident in rats that received 5.6 mg/kg. As shown in Fig. 1, rats injected with vehicle or 1.0 mg/kg MDMA starte ...
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Sexually dimorphic nucleus

The sexually dimorphic nucleus (SDN) is an ovoid, densely packed cluster of large cells located in the medial preoptic area (POA) of the hypothalamus which is believed to be related to sexual behavior in animals.Thus far, for all species of mammals investigated, the SDN has been repeatedly found to be considerably larger in males than in females. The volume of the SDN was 2.2 times as large in males as in females and contained 2.1 times as many cells. The human SDN was elongated in females and more spherical in males. No sex differences were observed in the human SDN in either cell density or mean diameter of the cell nuclei. The volume and cell number of the human SDN considerably decreases with age; although, the decrease in cell number is both sex and age-specific. In males, a substantial decrease in the cell number of the human SDN was observed between the age of 50-60 years. Cell death was more common in females than males, especially among those older than 70 years of age. The SDN cell number in females can drop to 10-15% that found in early childhood.SDN and its homologues exist widely in human, mammal, and some other animal brains, including: the third interstitial nucleus of the anterior hypothalamus (INAH3) in humans; ovine sexually dimorphic nucleus (oSDN) in the medial preoptic area/anterior hypothalamus (MPOA/AH) in sheep; sexually dimorphic nucleus in the preoptic area (SDN-POA) in rats; anterior hypothalamic nucleus (AHdc) in macaques; specific area in medial preoptic nucleus (POM) in quails; etc.↑ ↑ ↑ ↑
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