Life Inside a Microtubule
... Actin Modifications and the Cytoskeleton Actin, a highly expressed and ubiquitous cytoskeletal protein, is a major substrate for at least 17 post-translational modifications (PTMs)1. PTMs are highly dynamic and often reversible processes where a protein’s functional properties are altered by additio ...
... Actin Modifications and the Cytoskeleton Actin, a highly expressed and ubiquitous cytoskeletal protein, is a major substrate for at least 17 post-translational modifications (PTMs)1. PTMs are highly dynamic and often reversible processes where a protein’s functional properties are altered by additio ...
Adult Malnutrition - American Society for Parenteral and Enteral
... for evaluation. Reduced levels of visceral proteins can be viewed as potential markers of inflammation as the synthesis of these will fall in an inflammatory state. Clinical manifestations during the inflammatory response include, among others, elevated or depressed white blood cell count, significa ...
... for evaluation. Reduced levels of visceral proteins can be viewed as potential markers of inflammation as the synthesis of these will fall in an inflammatory state. Clinical manifestations during the inflammatory response include, among others, elevated or depressed white blood cell count, significa ...
Developmental Regulation of Genes Encoding Universal Stress
... tools can provide expression data to facilitate forward and reverse genetics approaches including RNA Interference (RNAi)23 and post-transcriptional regulation using microRNAs.24,25 The availability of the draft genome sequence of S. mansoni has led to the development of microarray slides based on o ...
... tools can provide expression data to facilitate forward and reverse genetics approaches including RNA Interference (RNAi)23 and post-transcriptional regulation using microRNAs.24,25 The availability of the draft genome sequence of S. mansoni has led to the development of microarray slides based on o ...
to Sample Chapter
... v. Awarded Nobel prize. i. Took interest in both proteins and nucleic acid at Cambridge in UK ii. Shared Nobel prize for medicine and physiology for cracking the genetic code. i. Most noted for one of two co-discoveries of the structure of DNA molecule. ii. Awarded Nobel prize i. Two times awarded N ...
... v. Awarded Nobel prize. i. Took interest in both proteins and nucleic acid at Cambridge in UK ii. Shared Nobel prize for medicine and physiology for cracking the genetic code. i. Most noted for one of two co-discoveries of the structure of DNA molecule. ii. Awarded Nobel prize i. Two times awarded N ...
Recycling of vitamin B12 and NAD+ within the Pdu
... Recent crystallography studies suggested that some Pdu MCP shell proteins, such as PduA, T and U, have pores that may mediate the transport of enzyme substrates/cofactors across the MCP shell. However, it’s possible that the cofactors might be regenerated in the local vicinity of the metabolic enzym ...
... Recent crystallography studies suggested that some Pdu MCP shell proteins, such as PduA, T and U, have pores that may mediate the transport of enzyme substrates/cofactors across the MCP shell. However, it’s possible that the cofactors might be regenerated in the local vicinity of the metabolic enzym ...
Conclusion - Federal Register of Legislation
... MIR162 expresses two novel proteins, Vip3Aa20 and PMI. The Vip3Aa20 protein is expressed at moderate levels in the corn grain, with a mean concentration of 43.6 µg/g fresh weight. The PMI protein is expressed at relatively low levels in the grain, with the mean concentration of 1.9 μg/g fresh weight ...
... MIR162 expresses two novel proteins, Vip3Aa20 and PMI. The Vip3Aa20 protein is expressed at moderate levels in the corn grain, with a mean concentration of 43.6 µg/g fresh weight. The PMI protein is expressed at relatively low levels in the grain, with the mean concentration of 1.9 μg/g fresh weight ...
Many-body van der Waals interactions in molecules and condensed
... In order to enable rational predictions and design of molecular and condensed-matter materials, including the interfaces between them, a reliable first-principles method is required that can describe vdW interactions both accurately and efficiently. However, forming an accurate description of vdW in ...
... In order to enable rational predictions and design of molecular and condensed-matter materials, including the interfaces between them, a reliable first-principles method is required that can describe vdW interactions both accurately and efficiently. However, forming an accurate description of vdW in ...
Structure of ATP-Bound Human ATP:Cobalamin
... interfaces cover 895 and 1450 Å2) and are mediated by conserved residues, thereby indicating that the formation of the trimer is required for activity. Although the presence of differently occupied active sites could indicate allostery within the trimer, we do not favor this possibility because supe ...
... interfaces cover 895 and 1450 Å2) and are mediated by conserved residues, thereby indicating that the formation of the trimer is required for activity. Although the presence of differently occupied active sites could indicate allostery within the trimer, we do not favor this possibility because supe ...
AraC Protein, Regulation of the L-arabinose Operon in Escherichia
... appears not to be a strong inhibitor of arabinose induction as it binds very weakly, which is consistent with Lee and colleagues finding that in addition to residues in the arabinose-binding pocket, residues some distance from the arabinose-binding pocket appear to interact with part of the IPTG mol ...
... appears not to be a strong inhibitor of arabinose induction as it binds very weakly, which is consistent with Lee and colleagues finding that in addition to residues in the arabinose-binding pocket, residues some distance from the arabinose-binding pocket appear to interact with part of the IPTG mol ...
Universitat Autònoma de Barcelona
... Castillo V, Espargaró A, Gordo V, Vendrell J, Ventura S Proteomics, Dec 2010;10(23:4172-4185 5. Studies on bacterial inclusion bodies De Groot NS, Espargaró A, Morell M, Ventura S Future Microbiology, Aug 2008;3(4):423-435 6. Inclusion bodies: specificity in their aggregation process and amyloid-lik ...
... Castillo V, Espargaró A, Gordo V, Vendrell J, Ventura S Proteomics, Dec 2010;10(23:4172-4185 5. Studies on bacterial inclusion bodies De Groot NS, Espargaró A, Morell M, Ventura S Future Microbiology, Aug 2008;3(4):423-435 6. Inclusion bodies: specificity in their aggregation process and amyloid-lik ...
Proteases - Home - KSU Faculty Member websites
... of about 33,000 MW used in meat tenderizing, beer production and hydrolization of proteins in the food industry. • mammalian lysosomal Cathepsins (11) These intracellular cysteine proteases are involved in such diverse activities as blood clotting, cancer growth and metastasis and bone remodeling. S ...
... of about 33,000 MW used in meat tenderizing, beer production and hydrolization of proteins in the food industry. • mammalian lysosomal Cathepsins (11) These intracellular cysteine proteases are involved in such diverse activities as blood clotting, cancer growth and metastasis and bone remodeling. S ...
ANSWERS TO CASE STUDIES Chapter 2: Drug Design and
... contains a tertiary amine and an alcohol that are hydrophilic. It contains a couple of functional groups that have mixed hydrophobic/hydrophilic character (ethers) and a fair ...
... contains a tertiary amine and an alcohol that are hydrophilic. It contains a couple of functional groups that have mixed hydrophobic/hydrophilic character (ethers) and a fair ...
Identification of proteins localized to the contractile vacuole of
... membranes (Allen and Naitoh 2002). The spongiome is divided into numerous vesicles and tubules. The spongiome can only fuse with the membrane of the second compartment and contains a V-H+-ATPase that provides an electrochemical gradient of protons for water transport. The spongiome is a fluid storin ...
... membranes (Allen and Naitoh 2002). The spongiome is divided into numerous vesicles and tubules. The spongiome can only fuse with the membrane of the second compartment and contains a V-H+-ATPase that provides an electrochemical gradient of protons for water transport. The spongiome is a fluid storin ...
CARBOHYDRATE METABOLISM
... • The liver is a glucose buffer for the blood. Its activity assures constant glucose concentration (approx. 5mM) in the blood. o Glucokinase, a liver-specific enzyme whose biosynthesis is induced by insulin in response to high blood glucose levels, has a high Km (about 10 mM) and high Vmax for gluco ...
... • The liver is a glucose buffer for the blood. Its activity assures constant glucose concentration (approx. 5mM) in the blood. o Glucokinase, a liver-specific enzyme whose biosynthesis is induced by insulin in response to high blood glucose levels, has a high Km (about 10 mM) and high Vmax for gluco ...
Production of a Brassica napus Low-Molecular
... showed a slight but statistically significant increase in seed oil content, one line exhibited a significant decrease in oil content, and the remainder had unaltered seed oil content compared with wild-type seeds (data not shown). In order to further substantiate these results, an examination of the F ...
... showed a slight but statistically significant increase in seed oil content, one line exhibited a significant decrease in oil content, and the remainder had unaltered seed oil content compared with wild-type seeds (data not shown). In order to further substantiate these results, an examination of the F ...
Pyruvic Acid and Formic Acid Metabolism in Sarcina
... was 6.5 to 6.8. Growth at constant pH. Yeast extract (30 g.) and peptone (30 g.) in 2.8 litres of distilled water were autoclaved in a 5-litre aspirator fitted with a rubber stopper holding a glass pH electrode and ports for alkali addition and gas exit. A tap fitted near the base allowed samples to ...
... was 6.5 to 6.8. Growth at constant pH. Yeast extract (30 g.) and peptone (30 g.) in 2.8 litres of distilled water were autoclaved in a 5-litre aspirator fitted with a rubber stopper holding a glass pH electrode and ports for alkali addition and gas exit. A tap fitted near the base allowed samples to ...
What Are Next Generation Innovative Therapeutic Targets?
... have led to the discovery of ⬎1000 research targets (targeted by investigational agents only) (Zheng et al., 2006b). These targets have been derived from analysis of disease relevance, functional roles, expression profiles, and loss-of-function genetics between normal and disease states (Ryan and Pa ...
... have led to the discovery of ⬎1000 research targets (targeted by investigational agents only) (Zheng et al., 2006b). These targets have been derived from analysis of disease relevance, functional roles, expression profiles, and loss-of-function genetics between normal and disease states (Ryan and Pa ...
Structure-Function Studies of Bacteriophage P2 Integrase and Cox Protein
... phage, P4. P4 has gained the ability to adopt the P2 protein machinery to its own purposes. In this work the importance of the native size for biologically active integrase and Cox proteins has been determined. Further, structure-function analyses of the two proteins have been performed with focus o ...
... phage, P4. P4 has gained the ability to adopt the P2 protein machinery to its own purposes. In this work the importance of the native size for biologically active integrase and Cox proteins has been determined. Further, structure-function analyses of the two proteins have been performed with focus o ...
167 renal and small intestinal sodium
... Magagnin et al. 1993; Markovich et al. 1993). At present, more detailed information about the N-glycosylation sites likely to be used is only available for the type II Na+/Pi symporter NaPi-2 from the rat. Site-directed mutagenesis has shown that amino acid residues Asn-298 and Asn-328 (located with ...
... Magagnin et al. 1993; Markovich et al. 1993). At present, more detailed information about the N-glycosylation sites likely to be used is only available for the type II Na+/Pi symporter NaPi-2 from the rat. Site-directed mutagenesis has shown that amino acid residues Asn-298 and Asn-328 (located with ...
with Non-Insulin-dependent Diabetes Mellitus
... Subjects. 19 subjects with NIDDM and 19 normal subjects participated in the study. All were Caucasians and sedentary. The control subjects had normal fasting plasma glucose, normal blood pressure, and no family history ofdiabetes. Clinical data are presented in Table I. Individuals with NIDDM as def ...
... Subjects. 19 subjects with NIDDM and 19 normal subjects participated in the study. All were Caucasians and sedentary. The control subjects had normal fasting plasma glucose, normal blood pressure, and no family history ofdiabetes. Clinical data are presented in Table I. Individuals with NIDDM as def ...
Nucleotide Sequence of fruA, the Gene Specifying Enzyme IIfru of
... a 2.5 kb PvuII restriction fragment spanningfruA+,cloned on a plasmid, was determined. This fragment contained three open reading frames (ORFs) but only one complete ORF, 1689 base pairs long, which was preceded by a well-defined Shine-Dalgarno sequence and ended with a rho-independent transcription ...
... a 2.5 kb PvuII restriction fragment spanningfruA+,cloned on a plasmid, was determined. This fragment contained three open reading frames (ORFs) but only one complete ORF, 1689 base pairs long, which was preceded by a well-defined Shine-Dalgarno sequence and ended with a rho-independent transcription ...
Quantitative Comparison of the Hydrogen Bond
... JNC′ scalar correlations between H-bond donor and acceptor amino acids (Figure 1). These correlations clearly show that the H-bond patterns of the first antiparallel β-sheet and of the central R-helix are very similar under native state and A-state conditions. In contrast, the h3JNC′ correlations in ...
... JNC′ scalar correlations between H-bond donor and acceptor amino acids (Figure 1). These correlations clearly show that the H-bond patterns of the first antiparallel β-sheet and of the central R-helix are very similar under native state and A-state conditions. In contrast, the h3JNC′ correlations in ...
SBT202 - SibTech, Inc.
... Functional activity in tissue culture: The ability of EGF/Si to bind to and activate EGF receptor was tested in two tissue culture assays: stimulation of EGFR tyrosine autophosphorylation and competition with EGF-toxin fusion protein (SibTech product # SBT076.012) for binding to EGFR expressed on MD ...
... Functional activity in tissue culture: The ability of EGF/Si to bind to and activate EGF receptor was tested in two tissue culture assays: stimulation of EGFR tyrosine autophosphorylation and competition with EGF-toxin fusion protein (SibTech product # SBT076.012) for binding to EGFR expressed on MD ...
Protein–protein interaction
Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces.In fact, proteins are vital macromolecules, at both cellular and systemic levels, but they rarely act alone. Diverse essential molecular processes within a cell are carried out by molecular machines that are built from a large number of protein components organized by their PPIs. Indeed, these interactions are at the core of the entire interactomics system of any living cell and so, unsurprisingly, aberrant PPIs are on the basis of multiple diseases, such as Creutzfeld-Jacob, Alzheimer's disease, and cancer.PPIs have been studied from different perspectives: biochemistry, quantum chemistry, molecular dynamics, signal transduction, among others. All this information enables the creation of large protein interaction networks – similar to metabolic or genetic/epigenetic networks – that empower the current knowledge on biochemical cascades and disease pathogenesis, as well as provide putative new therapeutic targets.