Developmental changes in barley microRNA expression profiles
... barley development, miR168-3p and miR1432-5p levels increase while the 5’U-miR156-5p level decreases (with exception for the 2-week-old barley). We have identified two miR156-5p izomiRs (called 5’U-miR156-5p [20 nt] and 5’UU-miR156-5p [21 nt]), which were expressed differently during barley developm ...
... barley development, miR168-3p and miR1432-5p levels increase while the 5’U-miR156-5p level decreases (with exception for the 2-week-old barley). We have identified two miR156-5p izomiRs (called 5’U-miR156-5p [20 nt] and 5’UU-miR156-5p [21 nt]), which were expressed differently during barley developm ...
Full Text - J
... sample concentration. Determining the optimal conditions for the purification and storage of the toxins was the key step in characterizing them. It was found that ion-exchange chromatography, but not an ultrafiltration system, was suitable for concentrating toxins. We also found that the purified sa ...
... sample concentration. Determining the optimal conditions for the purification and storage of the toxins was the key step in characterizing them. It was found that ion-exchange chromatography, but not an ultrafiltration system, was suitable for concentrating toxins. We also found that the purified sa ...
Comparison of Genes Encoding Enzymes of Sterol Biosynthesis
... or cycloartenol are diverged at the step of SQE cyclization (Figure 1) [8]. Lanosterol, the first tetracyclic intermediate in animals and fungi is converted to cholesterol in vertebrates and to ergosterol in fungi; and cycloartenol, the plant-specific first tetracyclic intermediate is converted to c ...
... or cycloartenol are diverged at the step of SQE cyclization (Figure 1) [8]. Lanosterol, the first tetracyclic intermediate in animals and fungi is converted to cholesterol in vertebrates and to ergosterol in fungi; and cycloartenol, the plant-specific first tetracyclic intermediate is converted to c ...
DOCTORAL THESIS
... tremula x tremuloides xyloglucan endotransglycosylase, are members of the same enzymatic family and have highly homologous 3-dimensional structures. However, the enzymes exhibit different activities, one a hydrolase the other a transferase; different specificities, one accepts only linear glcosydic ...
... tremula x tremuloides xyloglucan endotransglycosylase, are members of the same enzymatic family and have highly homologous 3-dimensional structures. However, the enzymes exhibit different activities, one a hydrolase the other a transferase; different specificities, one accepts only linear glcosydic ...
YangSpr07
... proteins, such as fibrinogen, fibronectin, vitronectin, collagen, and laminin. The amino acid sequence Arg-Gly-Asp or RGD present on extracellular matrix proteins is known to be a requirement for binding to cell surface receptor proteins, the integrins. The binding of extracellular matrix proteins t ...
... proteins, such as fibrinogen, fibronectin, vitronectin, collagen, and laminin. The amino acid sequence Arg-Gly-Asp or RGD present on extracellular matrix proteins is known to be a requirement for binding to cell surface receptor proteins, the integrins. The binding of extracellular matrix proteins t ...
Electrophoresis Basi..
... neutral pH’s are either basic or acidic depending upon their AA composition. Most proteins placed into basic conditions become negatively charged. Acidic conditions cause most proteins to develop a positive charge. ...
... neutral pH’s are either basic or acidic depending upon their AA composition. Most proteins placed into basic conditions become negatively charged. Acidic conditions cause most proteins to develop a positive charge. ...
Hydrolysis of a Series of Synthetic Peptide Substrates by the Human
... The 3C proteins of several picornaviruses, including poliovirus, foot-and-mouth disease virus (FMDV) and encephatomyocarditis virus (EMCV), have been demonstrated to be cysteine-type proteinases, involved in the processing of the respective polyproteins expressed by the monocistronic RNA genome. Nuc ...
... The 3C proteins of several picornaviruses, including poliovirus, foot-and-mouth disease virus (FMDV) and encephatomyocarditis virus (EMCV), have been demonstrated to be cysteine-type proteinases, involved in the processing of the respective polyproteins expressed by the monocistronic RNA genome. Nuc ...
Prions as protein-based genetic elements. - Lindquist Lab
... and [URE3] arise in 1 per 105 to 107 cells, depending on the genetic background (2, 38, 105, 108, 176). The frequency at which [Het-s∗ ] converts to [Het-s] is estimated at less than 1 per 107 (8). Incubating cells at low temperatures increases the induction rate of [PSI+] and [URE3] somewhat (23, 2 ...
... and [URE3] arise in 1 per 105 to 107 cells, depending on the genetic background (2, 38, 105, 108, 176). The frequency at which [Het-s∗ ] converts to [Het-s] is estimated at less than 1 per 107 (8). Incubating cells at low temperatures increases the induction rate of [PSI+] and [URE3] somewhat (23, 2 ...
Journal of Microbiology and Biotechnology
... In this study, evidences of IAA intermediates of IPA, IAAld, ILA, IAA, and TOL in the culture supernatant and feeding experiments suggested the existence of the only IPA pathway for IAA biosynthesis of P. polymyxa E681 (Fig. 2). Results of the IAA intermediates accumulated in the E681 culture demons ...
... In this study, evidences of IAA intermediates of IPA, IAAld, ILA, IAA, and TOL in the culture supernatant and feeding experiments suggested the existence of the only IPA pathway for IAA biosynthesis of P. polymyxa E681 (Fig. 2). Results of the IAA intermediates accumulated in the E681 culture demons ...
Ana Maria da Silva Esteves Dissertation presented to obtain
... Archaeoglobus fulgidus with and without bound CDP-inositol was solved by X-ray crystallography to a resolution of 2.5 and 2.65 Å, respectively. The DIPPS domain comprises six transmembrane (TM) -helices and one α-helix parallel to the membrane plane, and forms a dimer via its transmembrane helices. ...
... Archaeoglobus fulgidus with and without bound CDP-inositol was solved by X-ray crystallography to a resolution of 2.5 and 2.65 Å, respectively. The DIPPS domain comprises six transmembrane (TM) -helices and one α-helix parallel to the membrane plane, and forms a dimer via its transmembrane helices. ...
ICoMST-Abstract_YUFU
... summarized in Fig. 1. There were overall 782 and 775 potential ACE-inhibitory peptides for released from alpha-1(I) and alpha-2(I) collagen, respectively. Papain (100), chymotrypsin C (91), and ficain (89) were demonstrated to be the most effective enzymes in producing the most ACE inhibitory peptid ...
... summarized in Fig. 1. There were overall 782 and 775 potential ACE-inhibitory peptides for released from alpha-1(I) and alpha-2(I) collagen, respectively. Papain (100), chymotrypsin C (91), and ficain (89) were demonstrated to be the most effective enzymes in producing the most ACE inhibitory peptid ...
CO2 and O2 Distribution in Rubisco Suggests the
... in the hexadecamer. The values were divided by the average solvent accessible surface area per residue during simulation (based on the size of accessibility area of each residue at every fourth ns) and were normalized per residue (Supporting Figure 2). The resulting binding strength is dependent on ...
... in the hexadecamer. The values were divided by the average solvent accessible surface area per residue during simulation (based on the size of accessibility area of each residue at every fourth ns) and were normalized per residue (Supporting Figure 2). The resulting binding strength is dependent on ...
BioFiles v5 n1 - Sigma
... carbohydrates, sialic acids are substituents of glycan structures, appearing most frequently as the non-reducing terminal molecules of N-glycans, O-glycans, and glycosylphosphatidylinositol (GPI) anchored proteins. Sialic acids also differ from other sugars in that they are less commonly utilized as ...
... carbohydrates, sialic acids are substituents of glycan structures, appearing most frequently as the non-reducing terminal molecules of N-glycans, O-glycans, and glycosylphosphatidylinositol (GPI) anchored proteins. Sialic acids also differ from other sugars in that they are less commonly utilized as ...
Structure and Function of Mammalian Ribosomes
... In our studies we concentrated our efforts on developing methods for the preparation of high yields of subunits, the majority of which are structurally and functionally intact. This then allows us to correlate function more quantitatively with structure. We wanted to minimize the harshness of the co ...
... In our studies we concentrated our efforts on developing methods for the preparation of high yields of subunits, the majority of which are structurally and functionally intact. This then allows us to correlate function more quantitatively with structure. We wanted to minimize the harshness of the co ...
ANP 204 Main Text - National Open University of Nigeria
... The enantiomers for threose and erythrose are arranged according to the manner in which the substituents are arranged about the asymmetric carbon atoms. The naming by l and d (for “dextrorotatory” and “Levorotatory”) is according to a convention established by Emil Fisher. The carbohydrates also exh ...
... The enantiomers for threose and erythrose are arranged according to the manner in which the substituents are arranged about the asymmetric carbon atoms. The naming by l and d (for “dextrorotatory” and “Levorotatory”) is according to a convention established by Emil Fisher. The carbohydrates also exh ...
Invariant amino acids essential for decoding function of polypeptide
... not due to damage of binding to the ribosome: two other activities are not affected at all and the GTPase activity of eRF3 which is entirely dependent on the binding of eRF1 and eRF3 to the ribosome as mentioned above remains as in the presence of the wild-type eRF1 (Figure 2). Most likely, invarian ...
... not due to damage of binding to the ribosome: two other activities are not affected at all and the GTPase activity of eRF3 which is entirely dependent on the binding of eRF1 and eRF3 to the ribosome as mentioned above remains as in the presence of the wild-type eRF1 (Figure 2). Most likely, invarian ...
Sequence Alignment
... alignment is represented as a score and the scores are ranked. Scoring matrices are used to calculate the score of the alignment base by base (DNA) or amino acid by amino acid (protein). The alignment score will be the sum of the scores for each position. ...
... alignment is represented as a score and the scores are ranked. Scoring matrices are used to calculate the score of the alignment base by base (DNA) or amino acid by amino acid (protein). The alignment score will be the sum of the scores for each position. ...
LS1a Fall 2014 Lab 6: Ribosomal Protein Translation (PyMOL lab #3)
... We will be looking primarily at the bacterial ribosome today, as it is smaller and slightly less complicated than the eukaryotic ribosome, allowing scientists to study it in far greater structural detail. Both the bacterial and eukaryotic ribosomes consist of a small subunit and a large subunit. The ...
... We will be looking primarily at the bacterial ribosome today, as it is smaller and slightly less complicated than the eukaryotic ribosome, allowing scientists to study it in far greater structural detail. Both the bacterial and eukaryotic ribosomes consist of a small subunit and a large subunit. The ...
Resource Guide
... • Colostrum should look and have the consistency of melted vanilla ice cream. • Runny, thin colostrum or those mixed with blood are bad. • Quality can be tested using a colostrometer to test the immunoglobin levels. • Bacterial counts can also be tested and should be less than 100,000cfu/mL (cfu ...
... • Colostrum should look and have the consistency of melted vanilla ice cream. • Runny, thin colostrum or those mixed with blood are bad. • Quality can be tested using a colostrometer to test the immunoglobin levels. • Bacterial counts can also be tested and should be less than 100,000cfu/mL (cfu ...
Interactions of TCA cycle enzymes and of the CcpA
... enzymes malate dehydrogenase Mdh, isocitrate dehydrogenase Icd and citrate synthase CitZ form the core of a TCA cycle metabolon in B. subtilis. Therefore, in this work, the interactions between these enzymes and their dynamics were characterized in vitro by surface plasmon resonance (SPR). Moreover, ...
... enzymes malate dehydrogenase Mdh, isocitrate dehydrogenase Icd and citrate synthase CitZ form the core of a TCA cycle metabolon in B. subtilis. Therefore, in this work, the interactions between these enzymes and their dynamics were characterized in vitro by surface plasmon resonance (SPR). Moreover, ...
Plant Peroxisomes: Biogenesis and Function
... involved in numerous processes, including primary and secondary metabolism, development, and responses to abiotic and biotic stresses. Considerable progress has been made in the identification of factors involved in peroxisomal biogenesis, revealing mechanisms that are both shared with and diverged f ...
... involved in numerous processes, including primary and secondary metabolism, development, and responses to abiotic and biotic stresses. Considerable progress has been made in the identification of factors involved in peroxisomal biogenesis, revealing mechanisms that are both shared with and diverged f ...
Effects of Molecular Crowding on Binding Affinity of Dihydrofolate to
... The enzyme dihydrofolate reductase (DHFR) is a critical enzyme in the biosynthetic pathway for nucleotides and proteins in the cell. DHFR contributes to the production of purines by forming tetrahydrofolate (THF) using dihydrofolate (DHF) as the reactant and NADPH as the cofactor. Furthermore, tetra ...
... The enzyme dihydrofolate reductase (DHFR) is a critical enzyme in the biosynthetic pathway for nucleotides and proteins in the cell. DHFR contributes to the production of purines by forming tetrahydrofolate (THF) using dihydrofolate (DHF) as the reactant and NADPH as the cofactor. Furthermore, tetra ...
1 MCM10 MEDIATES THE INTERACTION BETWEEN DNA
... defect both at the telomere (Figure 1A) as well as at the HMR (Figure 1B). This observation raised the possibility that some pre-RC proteins may interact with silencing factors. Indeed, when tested in a two-hybrid system using LexA binding domain (BTM) and Gal4 activation domain (GAD) fusion protein ...
... defect both at the telomere (Figure 1A) as well as at the HMR (Figure 1B). This observation raised the possibility that some pre-RC proteins may interact with silencing factors. Indeed, when tested in a two-hybrid system using LexA binding domain (BTM) and Gal4 activation domain (GAD) fusion protein ...
Identification and characterization of novel interaction
... In Gram-positiven Bakterien mit niedrigem GC-Gehalt, wie Bacillus subtilis, wird KohlenstoffKatabolitenregulation überwiegend vom Katabolit Kontroll Protein A (CcpA), einem globalen Transkriptionsregulator, vermittelt. CcpA reprimiert oder aktiviert die Expression von hunderten von ...
... In Gram-positiven Bakterien mit niedrigem GC-Gehalt, wie Bacillus subtilis, wird KohlenstoffKatabolitenregulation überwiegend vom Katabolit Kontroll Protein A (CcpA), einem globalen Transkriptionsregulator, vermittelt. CcpA reprimiert oder aktiviert die Expression von hunderten von ...
Protein–protein interaction
Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces.In fact, proteins are vital macromolecules, at both cellular and systemic levels, but they rarely act alone. Diverse essential molecular processes within a cell are carried out by molecular machines that are built from a large number of protein components organized by their PPIs. Indeed, these interactions are at the core of the entire interactomics system of any living cell and so, unsurprisingly, aberrant PPIs are on the basis of multiple diseases, such as Creutzfeld-Jacob, Alzheimer's disease, and cancer.PPIs have been studied from different perspectives: biochemistry, quantum chemistry, molecular dynamics, signal transduction, among others. All this information enables the creation of large protein interaction networks – similar to metabolic or genetic/epigenetic networks – that empower the current knowledge on biochemical cascades and disease pathogenesis, as well as provide putative new therapeutic targets.