Diacylglycerol kinase zeta in hypothalamus interacts with long form leptin receptor. Relation to dietary fat and body weight regulation
... Identification and Cloning of DGK—Hypothalamus from 10 rats on a high-fat (60%) or low-fat (10%) diet were dissected and pooled for the purification of mRNA, which was then used for representational difference analysis as described (33). The cDNA fragment of rat Ob-Rbc, obtained by RT-PCR with prim ...
... Identification and Cloning of DGK—Hypothalamus from 10 rats on a high-fat (60%) or low-fat (10%) diet were dissected and pooled for the purification of mRNA, which was then used for representational difference analysis as described (33). The cDNA fragment of rat Ob-Rbc, obtained by RT-PCR with prim ...
Molecular networks in skeletal muscle plasticity
... depending on use as well as internal and external cues. In humans, low-load endurance-type exercise leads to qualitative changes of muscle tissue characterized by an increase in structures supporting oxygen delivery and consumption, such as capillaries and mitochondria. High-load strength-type exerc ...
... depending on use as well as internal and external cues. In humans, low-load endurance-type exercise leads to qualitative changes of muscle tissue characterized by an increase in structures supporting oxygen delivery and consumption, such as capillaries and mitochondria. High-load strength-type exerc ...
Crosslinking Technical Handbook
... contain reactive ends to specific functional groups (primary amines, sulfhydryls, etc.) on proteins or other molecules. The availability of several chemical groups in proteins and peptides make them targets for conjugation and for study using crosslinking methods. Crosslinkers also are commonly used ...
... contain reactive ends to specific functional groups (primary amines, sulfhydryls, etc.) on proteins or other molecules. The availability of several chemical groups in proteins and peptides make them targets for conjugation and for study using crosslinking methods. Crosslinkers also are commonly used ...
LCAT activation by apoA-I in HDL3 exposed to HOCl or MPO
... SAA1 and SAA2 • Acute-phase proteins – Synthesized by liver in response to inflammatory stimuli • SAA4 – Constitutively synthesized • Increased plasma levels predict CAD events • Apolipoproteins – Associate primarily with HDL ...
... SAA1 and SAA2 • Acute-phase proteins – Synthesized by liver in response to inflammatory stimuli • SAA4 – Constitutively synthesized • Increased plasma levels predict CAD events • Apolipoproteins – Associate primarily with HDL ...
Chondroitinase ABC I from Proteus vulgaris: cloning, recombinant
... been reported previously that the active form of cABC I isolated from P. vulgaris is missing the N-terminal signal sequence [9]. Therefore two 5 end primers were designed so as to generate a full-length clone and a truncated version of the gene by omitting 72 bases encoding the signal sequence. In ...
... been reported previously that the active form of cABC I isolated from P. vulgaris is missing the N-terminal signal sequence [9]. Therefore two 5 end primers were designed so as to generate a full-length clone and a truncated version of the gene by omitting 72 bases encoding the signal sequence. In ...
REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA
... acyltransferase family. However, the recent report of the 3-D structures of CrAT and COT has provided valuable insights into the molecular basis of substrate specificity and catalytic activity in the acyltransferase family (Jogl 2003, Jogl 2005). ...
... acyltransferase family. However, the recent report of the 3-D structures of CrAT and COT has provided valuable insights into the molecular basis of substrate specificity and catalytic activity in the acyltransferase family (Jogl 2003, Jogl 2005). ...
Studying the Interaction Profiles of Nonnatural Amino Acids –
... sites, as found in enzymes or proteins that carry oxygen and they regulate the function of DNA. The omnipresence of peptides and proteins in biological systems includes a wide variety of processes responsible for biological disorders and diseases.3,4 These can be either caused by defective proteins ...
... sites, as found in enzymes or proteins that carry oxygen and they regulate the function of DNA. The omnipresence of peptides and proteins in biological systems includes a wide variety of processes responsible for biological disorders and diseases.3,4 These can be either caused by defective proteins ...
The demonstration of nickel in the urease of Helicobacter pylori by
... mass of 89 kDa, and as the molecular mass of the urease is greater than 500 kDa, then the suggested structure is hexameric. This would indicate that the probable number of nickel atoms is six. These results support the model of six copies of each of the two polypeptides in the native enzyme suggeste ...
... mass of 89 kDa, and as the molecular mass of the urease is greater than 500 kDa, then the suggested structure is hexameric. This would indicate that the probable number of nickel atoms is six. These results support the model of six copies of each of the two polypeptides in the native enzyme suggeste ...
The Three-Dimensional Structure of Aspergillus niger Pectin Lyase
... 4°C. The diffraction patterns of PLB crystals were consistent with the orthorhombic space group P21212, with unit cell parameters of a 5 83.60 Å, b 5 88.80 Å, and c 5 42.28 Å. Assuming one molecule per asymmetric unit, the Vm and solvent content were estimated to be 2.07 Å3/D and 41%, respectively ( ...
... 4°C. The diffraction patterns of PLB crystals were consistent with the orthorhombic space group P21212, with unit cell parameters of a 5 83.60 Å, b 5 88.80 Å, and c 5 42.28 Å. Assuming one molecule per asymmetric unit, the Vm and solvent content were estimated to be 2.07 Å3/D and 41%, respectively ( ...
Metabolic significance of inorganic triphosphate, thiamine
... specific for PPPi plays only a minor role in the cytosolic PPPase activity, while most of the activity is due to inorganic pyrophosphatase. In animal tissues, most PPPase activity is due to the short-chain exopolyphosphatase prune, which hydrolyzes PPPi with high catalytic efficiency. We hypothesize ...
... specific for PPPi plays only a minor role in the cytosolic PPPase activity, while most of the activity is due to inorganic pyrophosphatase. In animal tissues, most PPPase activity is due to the short-chain exopolyphosphatase prune, which hydrolyzes PPPi with high catalytic efficiency. We hypothesize ...
Supplemental Table 3
... [evidence IEA]; Catalysis of the hydrolysis of peptide bonds by a mechanism in which water acts as a nucleophile, one or two metal ions hold the water molecule in place, and charged amino acid side chains are ligands for the metal ions [goid 8237] [evidence IEA]; Interacting selectively with zinc (Z ...
... [evidence IEA]; Catalysis of the hydrolysis of peptide bonds by a mechanism in which water acts as a nucleophile, one or two metal ions hold the water molecule in place, and charged amino acid side chains are ligands for the metal ions [goid 8237] [evidence IEA]; Interacting selectively with zinc (Z ...
22nd EMC Full Program - 25th Enzyme Mechanisms Conference
... pose challenges for downstream hit-validation and lead identification and different approaches are required to identify legitimate small molecule series that inhibit or activate the selected target. This presentation will focus on approaches taken that increase the probability of success in identify ...
... pose challenges for downstream hit-validation and lead identification and different approaches are required to identify legitimate small molecule series that inhibit or activate the selected target. This presentation will focus on approaches taken that increase the probability of success in identify ...
International Journal on New Trends in Education and Their
... …“mRNAs come as triple codons. This slips inside the ribosome until it comes across the stopper codon. mRNA slips. tRNAs are synthesized through mRNAs. Afterwards, a peptide bond is created between tRNAs. Considering the expression of the pre-service teacher, it can be inferred that he/she assumes t ...
... …“mRNAs come as triple codons. This slips inside the ribosome until it comes across the stopper codon. mRNA slips. tRNAs are synthesized through mRNAs. Afterwards, a peptide bond is created between tRNAs. Considering the expression of the pre-service teacher, it can be inferred that he/she assumes t ...
Metabolic decision making by protein-metabolite - ETH E
... analysis, we obtain a quantitative picture of the relationship between metabolic fluxes, metabolites, and proteins, at single reaction resolution. We find that this metabolic response is largely established by two mechanisms, namely an approximate transcriptional program, which rarely co ...
... analysis, we obtain a quantitative picture of the relationship between metabolic fluxes, metabolites, and proteins, at single reaction resolution. We find that this metabolic response is largely established by two mechanisms, namely an approximate transcriptional program, which rarely co ...
Retention of the Cis Proline Conformation in Tripeptide Fragments of
... hindered residue as the central one in tripeptides that are suitable for fragment coupling to prepare synthetic proteins. Based on the sequences of residues 92-94 (Tyr-Pro-Asn:YPN) and 113-115 (Asn-Pro-Tyr: NPY) in bovine pancreatic ribonuclease A (RNase A), in which the X-Pro peptide groups are in ...
... hindered residue as the central one in tripeptides that are suitable for fragment coupling to prepare synthetic proteins. Based on the sequences of residues 92-94 (Tyr-Pro-Asn:YPN) and 113-115 (Asn-Pro-Tyr: NPY) in bovine pancreatic ribonuclease A (RNase A), in which the X-Pro peptide groups are in ...
poultry meal vs poultry byproduct meal
... poultry ingredients are of equal quality. There are substantial differences between different grades within each of these ingredients. These differences may be due to differences in processing or to differences in the quality of the parts used to make the ingredient. The process used to produce both ...
... poultry ingredients are of equal quality. There are substantial differences between different grades within each of these ingredients. These differences may be due to differences in processing or to differences in the quality of the parts used to make the ingredient. The process used to produce both ...
Single-Molecule Fluorescence Resonance Energy Transfer
... three-way junction) with donor and acceptor attached to ends of two arms. In the open form (right side), two dyes are far from each other and there is little energy transfer. Therefore, donor excitation by the laser results in primarily donor’s emission. In the closed form (left side), two dyes are ...
... three-way junction) with donor and acceptor attached to ends of two arms. In the open form (right side), two dyes are far from each other and there is little energy transfer. Therefore, donor excitation by the laser results in primarily donor’s emission. In the closed form (left side), two dyes are ...
The C-terminal domain of the Rhizobium leguminosarum
... in the presence of hesperetin. Derivatives of pIJ1089 and pIJ1887 containing TnphoA were transferred to R. leguminosarum strain 8401 by conjugation. NodC–PhoA fusions expressing high levels of alkaline phosphatase could be identified in R. leguminosarum on plates containing hesperetin to induce nod ...
... in the presence of hesperetin. Derivatives of pIJ1089 and pIJ1887 containing TnphoA were transferred to R. leguminosarum strain 8401 by conjugation. NodC–PhoA fusions expressing high levels of alkaline phosphatase could be identified in R. leguminosarum on plates containing hesperetin to induce nod ...
Biochemical and physiological bases for utilization
... Protein is quantitatively the most expensive nutrient in swine diets. Hence it is imperative to understand the physiological roles played by amino acids in growth, development, lactation, reproduction, and health of pigs to improve their protein nutrition and reduce the costs of pork production. Due ...
... Protein is quantitatively the most expensive nutrient in swine diets. Hence it is imperative to understand the physiological roles played by amino acids in growth, development, lactation, reproduction, and health of pigs to improve their protein nutrition and reduce the costs of pork production. Due ...
Cytochromes P450 – importance of tissue specificity
... 14α-demetylase (CYP51) is one example of recycling between ER and cis-Golgi in somatic cells [14, 15] and transport through Golgi to the acrosome in male germ cells [15]. The exact reason for the static retention or recycling of cytochromes P450 is not fully understood. ER resident proteins must pos ...
... 14α-demetylase (CYP51) is one example of recycling between ER and cis-Golgi in somatic cells [14, 15] and transport through Golgi to the acrosome in male germ cells [15]. The exact reason for the static retention or recycling of cytochromes P450 is not fully understood. ER resident proteins must pos ...
Document
... modeling, showed that the second F coincided with Y75 in active aspartic proteinases, an important residue for catalytic activity.8 Therefore this residue was mutated to Y to create Bla g 2 Mut. Recent data derived from the crystal structure of Bla g 2 revealed that the first F was structurally equi ...
... modeling, showed that the second F coincided with Y75 in active aspartic proteinases, an important residue for catalytic activity.8 Therefore this residue was mutated to Y to create Bla g 2 Mut. Recent data derived from the crystal structure of Bla g 2 revealed that the first F was structurally equi ...
Phosphatidylglycerol-containing ER
... repair and restitution, and the demand to coordinate nuclear gene expression and maintain regulatory processes in response to cellular need for ATP synthesis were not plausible [3,4,16-18]. The mitochondrial oxidative phosphorylation system is dependent on both nuclear and mitochondrial DNA (mtDNA). ...
... repair and restitution, and the demand to coordinate nuclear gene expression and maintain regulatory processes in response to cellular need for ATP synthesis were not plausible [3,4,16-18]. The mitochondrial oxidative phosphorylation system is dependent on both nuclear and mitochondrial DNA (mtDNA). ...
purple Psyko writeup
... [indent]Leucine is a powerful branched chain amino acid (BCAA), of special importance for athletes because BCAA are metabolized in the muscle, rather than in the liver and can be used to build new proteins or as fuel to produce energy.[/indent] [b]Does Purple PSYKO™ replace proper nutrition, trainin ...
... [indent]Leucine is a powerful branched chain amino acid (BCAA), of special importance for athletes because BCAA are metabolized in the muscle, rather than in the liver and can be used to build new proteins or as fuel to produce energy.[/indent] [b]Does Purple PSYKO™ replace proper nutrition, trainin ...
University of Groningen Lactococcus lactis systems biology Eckhardt
... the p values of the t-test from a software package called Cyber-T 48. DNA microarrays allow high-throughput and are reasonably priced. They mostly examine expression of known annotated genes only. A more advanced microarray type is the tiling array ...
... the p values of the t-test from a software package called Cyber-T 48. DNA microarrays allow high-throughput and are reasonably priced. They mostly examine expression of known annotated genes only. A more advanced microarray type is the tiling array ...
Protein–protein interaction
Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces.In fact, proteins are vital macromolecules, at both cellular and systemic levels, but they rarely act alone. Diverse essential molecular processes within a cell are carried out by molecular machines that are built from a large number of protein components organized by their PPIs. Indeed, these interactions are at the core of the entire interactomics system of any living cell and so, unsurprisingly, aberrant PPIs are on the basis of multiple diseases, such as Creutzfeld-Jacob, Alzheimer's disease, and cancer.PPIs have been studied from different perspectives: biochemistry, quantum chemistry, molecular dynamics, signal transduction, among others. All this information enables the creation of large protein interaction networks – similar to metabolic or genetic/epigenetic networks – that empower the current knowledge on biochemical cascades and disease pathogenesis, as well as provide putative new therapeutic targets.