The Tobacco Homolog of Mammalian Calreticulin
... soluble ER resident proteins. For that purpose, microsomes were isolated from germinating tobacco seed 4 days after the start of imbibition because this tissue was previously shown to contain the highest BiP mRNA levels of all tobacco tissues analyzed (Denecke et al., 1991). Preliminary microsequenc ...
... soluble ER resident proteins. For that purpose, microsomes were isolated from germinating tobacco seed 4 days after the start of imbibition because this tissue was previously shown to contain the highest BiP mRNA levels of all tobacco tissues analyzed (Denecke et al., 1991). Preliminary microsequenc ...
Document
... # These centrifuges are run in batch to isolate small volumes of biological macromolecules or cells. ...
... # These centrifuges are run in batch to isolate small volumes of biological macromolecules or cells. ...
Anorexia Nervosa Anorexia Nervosa
... Purpose is to build a negative charge inside membrane and thus attract H+ ions back into the mitochondrion. (the membrane is impervious to H+ except for proton channels; therefore the protons have no choice but to operate the ATPase enzyme) Oxidative Phosphorylation: The conversion of ADP and inorga ...
... Purpose is to build a negative charge inside membrane and thus attract H+ ions back into the mitochondrion. (the membrane is impervious to H+ except for proton channels; therefore the protons have no choice but to operate the ATPase enzyme) Oxidative Phosphorylation: The conversion of ADP and inorga ...
Dynamics of Protein Tyrosine Nitration and
... different N-terminal inserts and microtubule binding repeats [65]. Endogenous tyrosine residues prone to be nitrated in tau protein are at 18, 29, 197, 310, and 394 position (numbers correspond to the longest tau isoform, which contains441 amino acids). In vitro, selectively of ONOO- mediated tyrosi ...
... different N-terminal inserts and microtubule binding repeats [65]. Endogenous tyrosine residues prone to be nitrated in tau protein are at 18, 29, 197, 310, and 394 position (numbers correspond to the longest tau isoform, which contains441 amino acids). In vitro, selectively of ONOO- mediated tyrosi ...
TARGET: a new method for predicting protein subcellular
... localization of the entire proteome (Kumar et al., 2002; Huh et al., 2003); however, such diligent feats are not practicable in all species. Therefore, experimental annotation of protein subcellular localization is not able to keep up with the large number of sequences that continue to emerge from t ...
... localization of the entire proteome (Kumar et al., 2002; Huh et al., 2003); however, such diligent feats are not practicable in all species. Therefore, experimental annotation of protein subcellular localization is not able to keep up with the large number of sequences that continue to emerge from t ...
Hepatitis C virus (HCV) - HAL
... convoluted electron-dense ER membranes were found to form multiple layers in these cells (Fig. 3). No HCV-LPs or marked clustering of lipid droplets was observed in cells producing this mutant core protein. These observations confirm that the ASC180/3/4VLV core protein remains anchored in the ER mem ...
... convoluted electron-dense ER membranes were found to form multiple layers in these cells (Fig. 3). No HCV-LPs or marked clustering of lipid droplets was observed in cells producing this mutant core protein. These observations confirm that the ASC180/3/4VLV core protein remains anchored in the ER mem ...
Carbon Compounds 2-3 Foldable Instructions
... & lipids. It turns red in the presence of fats & lipids. Benedict’s solution is an indicator solution for simple sugars. It changes from blue to yellow, orange or red. Iodine solution is an indicator solution for complex sugars. It changes from brown to blue ...
... & lipids. It turns red in the presence of fats & lipids. Benedict’s solution is an indicator solution for simple sugars. It changes from blue to yellow, orange or red. Iodine solution is an indicator solution for complex sugars. It changes from brown to blue ...
TRYPSIN / LYS
... Specificity: Serine endopeptidase that specifically hydrolyzes proteins and peptides at the carboxy side of the basic amino acids Arg and Lys. Amide and ester bonds of Arg and Lys are also cleaved. Optimum pH: 8.0 Description Trypsin specifically hydrolyzes peptide bonds at the carboxylic sides of l ...
... Specificity: Serine endopeptidase that specifically hydrolyzes proteins and peptides at the carboxy side of the basic amino acids Arg and Lys. Amide and ester bonds of Arg and Lys are also cleaved. Optimum pH: 8.0 Description Trypsin specifically hydrolyzes peptide bonds at the carboxylic sides of l ...
Sequencing the World of Possibilities for Energy & Environment
... Sequencing the World of Possibilities for Energy & Environment ...
... Sequencing the World of Possibilities for Energy & Environment ...
Biomacromolecules ppt
... Amino acids (monomers) bonded together by peptide bonds that form straight chains of Polymers(=polypeptide). Very large polypeptide = protein. Metabolic proteins are Enzymes. Peptide bond is formed by the Amino/Carboxyl sections of the Amino acid. The R group is responsible for the Hydrogen and ioni ...
... Amino acids (monomers) bonded together by peptide bonds that form straight chains of Polymers(=polypeptide). Very large polypeptide = protein. Metabolic proteins are Enzymes. Peptide bond is formed by the Amino/Carboxyl sections of the Amino acid. The R group is responsible for the Hydrogen and ioni ...
Solid Tumour Section Ovary: inv(10)(q11q11) in ovarian germ cell tumors
... Diagrammatic representation of RET/NCOA4 (PTC3) fusion protein. Amino acids surrounding each breakpoint (arrows) are indicated, and numbered according to their position within RET or NCOA4, respectively. Peptide sequences from RET are in red, from NCOA4 in green. The transmembrane domain (TM) of RET ...
... Diagrammatic representation of RET/NCOA4 (PTC3) fusion protein. Amino acids surrounding each breakpoint (arrows) are indicated, and numbered according to their position within RET or NCOA4, respectively. Peptide sequences from RET are in red, from NCOA4 in green. The transmembrane domain (TM) of RET ...
DOC
... animal) and to maximise growth performance under culture conditions. Understanding the physiological basis of observed growth in terms of anabolic and catabolic processes will then enable informed decisions to be made on the modification of diets and feeding regimes. The particular cost-effective fe ...
... animal) and to maximise growth performance under culture conditions. Understanding the physiological basis of observed growth in terms of anabolic and catabolic processes will then enable informed decisions to be made on the modification of diets and feeding regimes. The particular cost-effective fe ...
The phosphorylation of proteins: a major mechanism for biological
... interconversion reactions of phosphorylase came approximately 10 years after their discovery, when, in the 1950s, independent work on liver phosphorylase by Earl Sutherland (also a Cori student) and muscle phosphorylase by E. H . Fischer and myself (Fischer & Krebs, 1955; Sutherland & Wosilait, 1955 ...
... interconversion reactions of phosphorylase came approximately 10 years after their discovery, when, in the 1950s, independent work on liver phosphorylase by Earl Sutherland (also a Cori student) and muscle phosphorylase by E. H . Fischer and myself (Fischer & Krebs, 1955; Sutherland & Wosilait, 1955 ...
COT102 Decision Document draft only
... Syngenta has developed product Cotton COT102 which was genetically modified using Agrobacterium tumefaciens-mediated transformation. It contains two novel genes: vip3A(a) gene from B. thuringiensis strain AB88 coding for the vegetative insecticidal protein VIP3A, and the aph4 gene from E. coli, codi ...
... Syngenta has developed product Cotton COT102 which was genetically modified using Agrobacterium tumefaciens-mediated transformation. It contains two novel genes: vip3A(a) gene from B. thuringiensis strain AB88 coding for the vegetative insecticidal protein VIP3A, and the aph4 gene from E. coli, codi ...
Initial characterization of ayrRABC
... he proper localization of many proteins requires their translocation across one or more membranes. The general secretory (Sec) pathway, conserved throughout bacteria, is the canonical translocation pathway and is responsible for translocating the vast majority of secreted proteins across the cytopla ...
... he proper localization of many proteins requires their translocation across one or more membranes. The general secretory (Sec) pathway, conserved throughout bacteria, is the canonical translocation pathway and is responsible for translocating the vast majority of secreted proteins across the cytopla ...
Plant–pathogen interactions: what is proteomics telling us?
... main secretion system used by pathogenic bacteria during infection is the type III secretion system (TTSS), which is involved in some of the most devastating diseases in animals and plants (for a review, see [15]). This system enables bacteria to directly inject proteins, called effectors or virulen ...
... main secretion system used by pathogenic bacteria during infection is the type III secretion system (TTSS), which is involved in some of the most devastating diseases in animals and plants (for a review, see [15]). This system enables bacteria to directly inject proteins, called effectors or virulen ...
Self-Interaction of the Herpes Simplex Virus Type 1
... involved in multimerization. Mutant protein R9DS13 was resolved as a 35-kDa species, but the level of expression, measured both by immunoprecipitation with polyclonal antibody and by Western blot analysis (data not shown), was extremely low compared to the wild-type protein and to the other mutant p ...
... involved in multimerization. Mutant protein R9DS13 was resolved as a 35-kDa species, but the level of expression, measured both by immunoprecipitation with polyclonal antibody and by Western blot analysis (data not shown), was extremely low compared to the wild-type protein and to the other mutant p ...
CHAPTER 6
... Phospholipase C-b is activated specifically by Gq, a GTP-binding protein, and also by Ca2+. ...
... Phospholipase C-b is activated specifically by Gq, a GTP-binding protein, and also by Ca2+. ...
1 - chem.msu.su
... One common denominator in signal transductions—whether they involve adenylate cyclase, a transmembrane receptor-tyrosine kinase, phospholipase C, or an ion channel—is the eventual regulation of the activity of a protein kinase. We have seen examples of kinases activated by cAMP, insulin, Ca2+/calmod ...
... One common denominator in signal transductions—whether they involve adenylate cyclase, a transmembrane receptor-tyrosine kinase, phospholipase C, or an ion channel—is the eventual regulation of the activity of a protein kinase. We have seen examples of kinases activated by cAMP, insulin, Ca2+/calmod ...
Student PPT Notes
... http://healthland.time.com/2013/11/07/7-foods-that-wont-bethe-same-if-trans-fats-are-banned/ - explains trans fats and shows examples of fods that contain them ...
... http://healthland.time.com/2013/11/07/7-foods-that-wont-bethe-same-if-trans-fats-are-banned/ - explains trans fats and shows examples of fods that contain them ...
Calling names
... • Glutamine synthetase - 12 subunits of 468 residues each - total mol. wt. of 600,000 • Connectin proteins - alpha - MW 2.8 million! ...
... • Glutamine synthetase - 12 subunits of 468 residues each - total mol. wt. of 600,000 • Connectin proteins - alpha - MW 2.8 million! ...
Gene Section S100A4 (S100 calcium binding protein A4)
... 101 aminoacid residues and is approx 12 kDa in size. In common with most S100 family members, S100A4 is an antiparallel homodimer stabilised by noncovalent interactions between two helices from each subunit forming an X-type four-helix bundle. Each subunit has two calcium-binding EF-hands linked by ...
... 101 aminoacid residues and is approx 12 kDa in size. In common with most S100 family members, S100A4 is an antiparallel homodimer stabilised by noncovalent interactions between two helices from each subunit forming an X-type four-helix bundle. Each subunit has two calcium-binding EF-hands linked by ...
A + U, G + C
... DNA is inherited from parents Proteins are made of amino acids DNA is usually in the nucleus (bacteria don’t have a nucleus) DNA codes for proteins ...
... DNA is inherited from parents Proteins are made of amino acids DNA is usually in the nucleus (bacteria don’t have a nucleus) DNA codes for proteins ...
Welcome to Our Microbial Genetics Class
... complex and not completely understood, but catabolite repression or the glucose effect probably plays a part. The enzymes for glucose catabolism are constitutive and unaffected by CAP activity. When the bacterium is given glucose, the cAMP level drops, resulting in deactivation of the catabolite act ...
... complex and not completely understood, but catabolite repression or the glucose effect probably plays a part. The enzymes for glucose catabolism are constitutive and unaffected by CAP activity. When the bacterium is given glucose, the cAMP level drops, resulting in deactivation of the catabolite act ...
... The basic steps for cloning the coding region of the protein of interest into a fusion protein expression vector are outlined in Table 2. Tools available for each step of the process are indicated. Use of the HaloTag® Flexi® Vector System simplifies the procedure, and a free webbased tool for primer ...
Protein–protein interaction
Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces.In fact, proteins are vital macromolecules, at both cellular and systemic levels, but they rarely act alone. Diverse essential molecular processes within a cell are carried out by molecular machines that are built from a large number of protein components organized by their PPIs. Indeed, these interactions are at the core of the entire interactomics system of any living cell and so, unsurprisingly, aberrant PPIs are on the basis of multiple diseases, such as Creutzfeld-Jacob, Alzheimer's disease, and cancer.PPIs have been studied from different perspectives: biochemistry, quantum chemistry, molecular dynamics, signal transduction, among others. All this information enables the creation of large protein interaction networks – similar to metabolic or genetic/epigenetic networks – that empower the current knowledge on biochemical cascades and disease pathogenesis, as well as provide putative new therapeutic targets.