INSILICO APPROACHES TOWARDS THE DRUG TARGET AURORKINASES USING THE ORTHO
... chromatid segregation. Defects in this segregation can cause genetic instability, a condition which is highly associated with tumorigenesis[1]. Aurora family kinases play roles in several mitotic processes, including the G2/M transition, mitotic spindle organization, a regulatory domain in the NH2 t ...
... chromatid segregation. Defects in this segregation can cause genetic instability, a condition which is highly associated with tumorigenesis[1]. Aurora family kinases play roles in several mitotic processes, including the G2/M transition, mitotic spindle organization, a regulatory domain in the NH2 t ...
Structure and function of proteins controlling strain
... All of the R genes discussed in this review show a high level of recognitional specificity, that is, they provide resistance against only some strains of a particular pathogen species. This is principally the result of a high level of polymorphism among pathogen avirulence genes which encode the pro ...
... All of the R genes discussed in this review show a high level of recognitional specificity, that is, they provide resistance against only some strains of a particular pathogen species. This is principally the result of a high level of polymorphism among pathogen avirulence genes which encode the pro ...
TIM BARREL
... Out of the 200 residues required to form a TIM barrel, only 160 are considered structurally equivalent between different proteins sharing this fold. The remaining residues are located on the loop regions that link the helices and sheets; the loops at the Cterminal end of the sheets tend to contain t ...
... Out of the 200 residues required to form a TIM barrel, only 160 are considered structurally equivalent between different proteins sharing this fold. The remaining residues are located on the loop regions that link the helices and sheets; the loops at the Cterminal end of the sheets tend to contain t ...
Summary Affimer reagents in affinity resins Specific target binding
... repeated injection and elution of pure hIgG (81 runs), including 19 clean-inplace (CIP) cycles (100 mM NaOH, 10 min). The data indicate consistent capture performance over the course of this study (Figure 4A). No leaching from Affimer columns was detected by western blotting of concentrated ...
... repeated injection and elution of pure hIgG (81 runs), including 19 clean-inplace (CIP) cycles (100 mM NaOH, 10 min). The data indicate consistent capture performance over the course of this study (Figure 4A). No leaching from Affimer columns was detected by western blotting of concentrated ...
Ribosome
... linked together by the ribosome. One by one, they bind to the ribosome, add their amino acid to the chain, and then depart 50S and 30S made primarily of RNA and are both larger than the average protein. 50S has rather rigid structure, while 30S has fairly ...
... linked together by the ribosome. One by one, they bind to the ribosome, add their amino acid to the chain, and then depart 50S and 30S made primarily of RNA and are both larger than the average protein. 50S has rather rigid structure, while 30S has fairly ...
Structure-Function Analysis of Mouse Interferon Alpha Species
... Antiviral properties of the MulFN-~IO protein To obtain information on the antiviral properties of the MulFN-~10 protein, the gene was inserted into the eukaryotic expression vector pSV328A (Van Heuvel et al., 1986) which contains the origin of replication and early promoter of simian virus 40 (SV40 ...
... Antiviral properties of the MulFN-~IO protein To obtain information on the antiviral properties of the MulFN-~10 protein, the gene was inserted into the eukaryotic expression vector pSV328A (Van Heuvel et al., 1986) which contains the origin of replication and early promoter of simian virus 40 (SV40 ...
The role of carbohydrate in sperm
... affinities for their substrates, e.g. the arabinose binding protein has a K
... affinities for their substrates, e.g. the arabinose binding protein has a K
Electrophilic Affibodies Forming Covalent Bonds to Protein Targets*DS
... result of the kinetics of B cell stimulation (11). In vitro selection methods based on phage display (12), mRNA/ribosome display (13), or yeast cell surface display (14) have sometimes managed to obtain femtomolar affinity antibodies. There are a few examples of antibodies that form covalent bonds t ...
... result of the kinetics of B cell stimulation (11). In vitro selection methods based on phage display (12), mRNA/ribosome display (13), or yeast cell surface display (14) have sometimes managed to obtain femtomolar affinity antibodies. There are a few examples of antibodies that form covalent bonds t ...
The Crystal Structure of Arabidopsis thaliana Allene Oxide Cyclase
... 12,13(S)-epoxylinolenic acid but not 12,13(S)-epoxylinoleic acid as a substrate (Ziegler et al., 1999). This is in contrast with AOS, which produces both allene oxides from the respective 13(S)hydroperoxy fatty acids (18:3 and 18:2, respectively). It thus appears that AOC confers additional specific ...
... 12,13(S)-epoxylinolenic acid but not 12,13(S)-epoxylinoleic acid as a substrate (Ziegler et al., 1999). This is in contrast with AOS, which produces both allene oxides from the respective 13(S)hydroperoxy fatty acids (18:3 and 18:2, respectively). It thus appears that AOC confers additional specific ...
nature of polyethyleneimine-glucose oxidase interactions
... Based on the best docked energies: -5.8 and -4.5 kcal/mol, two sites were differentiated on the protein body, which show the best affinity to PEI (Table 1). In the first case, the ligand is bound to amino acids inside of the protein area (LIG1, Figure 5), while in the second case the binding takes p ...
... Based on the best docked energies: -5.8 and -4.5 kcal/mol, two sites were differentiated on the protein body, which show the best affinity to PEI (Table 1). In the first case, the ligand is bound to amino acids inside of the protein area (LIG1, Figure 5), while in the second case the binding takes p ...
EVIDENCE FOR TWO DISTINCT CLASSES OF STREPTOCOCCAL
... conserved domain on their surface give a positive serum opacity reaction, whereas nearly all isolates possessing the conserved domain fail to produce OF. Based on these fundamental differences, we propose that most group A streptococcal serotypes fall into one of two major classes of M protein, whic ...
... conserved domain on their surface give a positive serum opacity reaction, whereas nearly all isolates possessing the conserved domain fail to produce OF. Based on these fundamental differences, we propose that most group A streptococcal serotypes fall into one of two major classes of M protein, whic ...
Title goes here - VideoLectures.NET
... Then its functional class is “Cell processes, Transport/binding proteins” 12/13 (86%) correct on Test Set - probability of this result occurring by chance is estimated at 4x10-7. 24 ORFs of unknown function are predicted by the rule. ...
... Then its functional class is “Cell processes, Transport/binding proteins” 12/13 (86%) correct on Test Set - probability of this result occurring by chance is estimated at 4x10-7. 24 ORFs of unknown function are predicted by the rule. ...
Package `PPInfer`
... Title Inferring functionally related proteins using protein interaction networks Description Interactions between proteins occur in many, if not most, biological processes. Most proteins perform their functions in networks associated with other proteins and other biomolecules. This fact has motivate ...
... Title Inferring functionally related proteins using protein interaction networks Description Interactions between proteins occur in many, if not most, biological processes. Most proteins perform their functions in networks associated with other proteins and other biomolecules. This fact has motivate ...
Benchmarking homology detection procedures with low complexity
... of equally similar sequences found in a database search in the absence of homology. As a result, it is possible to determine whether two sequences can be seen as homologous with a given degree of confidence, under a given set of assumptions on evolutionary rates and similar properties. Many protein ...
... of equally similar sequences found in a database search in the absence of homology. As a result, it is possible to determine whether two sequences can be seen as homologous with a given degree of confidence, under a given set of assumptions on evolutionary rates and similar properties. Many protein ...
Chapter 23: Meat Emulsions - FEA
... homogenization; it forms a membrane that protects the globules against aggregation when they come close to each other (McClements 2005). In a number of cases, emulsifiers are necessary to facilitate emulsion formation (Schramm 2005). Many emulsifiers are amphiphilic molecules, with polar and nonpol ...
... homogenization; it forms a membrane that protects the globules against aggregation when they come close to each other (McClements 2005). In a number of cases, emulsifiers are necessary to facilitate emulsion formation (Schramm 2005). Many emulsifiers are amphiphilic molecules, with polar and nonpol ...
- Horizon Discovery
... NanoLuc® luciferase is a novel, small, bright luciferase reporter. It is one third the size and 100 times brighter than the most commonly used luciferase, firefly. These features enable the development of homogenous assays for primary and secondary screening, reporting on pathways via the endogenous ...
... NanoLuc® luciferase is a novel, small, bright luciferase reporter. It is one third the size and 100 times brighter than the most commonly used luciferase, firefly. These features enable the development of homogenous assays for primary and secondary screening, reporting on pathways via the endogenous ...
Machine learning methods for Protein Secondary Structure Prediction
... van der Waals interactions Bond angle/length constraints Long distance, e.g. ...
... van der Waals interactions Bond angle/length constraints Long distance, e.g. ...
Force Fields
... data set of 400 proteins. This is the null-model. But we count 3457 Ala-in-helix; that is 1.38 times ‘too many’. So the ‘score’ for (Ala,helix) = Pref(A,H)= ln(observed/predicted) = ln(3457/2505)=ln(1.38)=0.32. The preference parameter Pref(A,H) is positive. So, here positive is good (unlike ΔG or A ...
... data set of 400 proteins. This is the null-model. But we count 3457 Ala-in-helix; that is 1.38 times ‘too many’. So the ‘score’ for (Ala,helix) = Pref(A,H)= ln(observed/predicted) = ln(3457/2505)=ln(1.38)=0.32. The preference parameter Pref(A,H) is positive. So, here positive is good (unlike ΔG or A ...
Supplementary Figure 1
... evolution; therefore the branch lengths correspond to amino acid substitutions per time unit. Evidently, UbS27a domains are less conserved than ribosomal S27a domains or homologs to SUMO1, despite the presence of a hypervariable loop at the N-terminus of SUMO1. Particularly indicative of a high rate ...
... evolution; therefore the branch lengths correspond to amino acid substitutions per time unit. Evidently, UbS27a domains are less conserved than ribosomal S27a domains or homologs to SUMO1, despite the presence of a hypervariable loop at the N-terminus of SUMO1. Particularly indicative of a high rate ...
Astrovirus Replication: An Overview
... fragment of VP34 sequence and coat proteins from these viruses are shown in Fig. 3. Interestingly, the putative RNA-binding domain present at the N-terminus of VP34 was never included in positive alignments, confirming a different biological function for these two domains of the VP34 protein. The ev ...
... fragment of VP34 sequence and coat proteins from these viruses are shown in Fig. 3. Interestingly, the putative RNA-binding domain present at the N-terminus of VP34 was never included in positive alignments, confirming a different biological function for these two domains of the VP34 protein. The ev ...
Corn MON 88017 - Biotechnology Philippines
... possible using a vector containing both the cp4 epsps and cry3Bb1 genes . Glyphosate, the active ingredient in Roundup® agricultural herbicides, kills plants by inhibiting the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). This enzyme is a critical step in the shikimic acid pathway for ...
... possible using a vector containing both the cp4 epsps and cry3Bb1 genes . Glyphosate, the active ingredient in Roundup® agricultural herbicides, kills plants by inhibiting the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). This enzyme is a critical step in the shikimic acid pathway for ...
S1 Fig.
... though sufficiently far into the sample area, the solution is insensitive to the details of this boundary condition [6]. Equations (1-3) were solved to determine tissue PO2 using the finite element method, implemented using Matlab (The MathWorks Inc., Natick, MA) with a nonlinear solver based on Gau ...
... though sufficiently far into the sample area, the solution is insensitive to the details of this boundary condition [6]. Equations (1-3) were solved to determine tissue PO2 using the finite element method, implemented using Matlab (The MathWorks Inc., Natick, MA) with a nonlinear solver based on Gau ...
Module 17 / Microscopic Level—The Sarcomere
... relates to force generation; predict what effect changes in filam ent ov erlap would hav e on m uscle function. ...
... relates to force generation; predict what effect changes in filam ent ov erlap would hav e on m uscle function. ...
AIBSTCT Nucleic Acids Research - Walter Lab
... Fig2B Immunoprecipitation of SRP19. pSRP19 was transcribed from the SP6 promoter of pGem2 and the transcripts were translated in a wheat germ cell free translation system in the presence of 35S methionine and analysed on 10-15% SDS polyacrylamide gels. SRP19 protein was expressed either as a full-le ...
... Fig2B Immunoprecipitation of SRP19. pSRP19 was transcribed from the SP6 promoter of pGem2 and the transcripts were translated in a wheat germ cell free translation system in the presence of 35S methionine and analysed on 10-15% SDS polyacrylamide gels. SRP19 protein was expressed either as a full-le ...
Test 2
... The real difference comes in the fact that sugars don’t have to be in linear 164 linkages, many other linkages like 166 can occur and you can have one sugar with multiple linkages so branched structures are also possible. This greatly enlarges the number of different conformation states. There is al ...
... The real difference comes in the fact that sugars don’t have to be in linear 164 linkages, many other linkages like 166 can occur and you can have one sugar with multiple linkages so branched structures are also possible. This greatly enlarges the number of different conformation states. There is al ...
Protein–protein interaction
Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces.In fact, proteins are vital macromolecules, at both cellular and systemic levels, but they rarely act alone. Diverse essential molecular processes within a cell are carried out by molecular machines that are built from a large number of protein components organized by their PPIs. Indeed, these interactions are at the core of the entire interactomics system of any living cell and so, unsurprisingly, aberrant PPIs are on the basis of multiple diseases, such as Creutzfeld-Jacob, Alzheimer's disease, and cancer.PPIs have been studied from different perspectives: biochemistry, quantum chemistry, molecular dynamics, signal transduction, among others. All this information enables the creation of large protein interaction networks – similar to metabolic or genetic/epigenetic networks – that empower the current knowledge on biochemical cascades and disease pathogenesis, as well as provide putative new therapeutic targets.