Rapid Point of Care Molecular Diagnostic Test for Ebola
... Analytical sensitivity was determined by testing 10-fold serial dilutions of RNA extracted from Ebola virus – Zaire, Kikwit (1995) strain and a strain from the ongoing outbreak (Guinea-2014) in W. Africa. A true POC test for Ebola was developed, consisting of lyophilized amplification reagents, a ra ...
... Analytical sensitivity was determined by testing 10-fold serial dilutions of RNA extracted from Ebola virus – Zaire, Kikwit (1995) strain and a strain from the ongoing outbreak (Guinea-2014) in W. Africa. A true POC test for Ebola was developed, consisting of lyophilized amplification reagents, a ra ...
Administrative Office St. Joseph`s Hospital Site, L301
... passively-acquired maternal HCV antibody. A single negative RNA test should be interpreted with caution and cannot exclude infection as some individuals have low levels of viremia that can oscillate below the level of detection of a given assay. Quantitative tests are used to establish baseline pre- ...
... passively-acquired maternal HCV antibody. A single negative RNA test should be interpreted with caution and cannot exclude infection as some individuals have low levels of viremia that can oscillate below the level of detection of a given assay. Quantitative tests are used to establish baseline pre- ...
equine infectious anaemia
... Identification of the agent: Virus from a horse can be isolated by inoculating suspect blood into a susceptible horse or on to leukocyte cultures prepared from susceptible horses. Recognition of infection in horses that have been inoculated experimentally may be made on the basis of clinical signs, ...
... Identification of the agent: Virus from a horse can be isolated by inoculating suspect blood into a susceptible horse or on to leukocyte cultures prepared from susceptible horses. Recognition of infection in horses that have been inoculated experimentally may be made on the basis of clinical signs, ...
Viral Meningitis_final.pmd
... difficulty waking the baby and refusal to eat may be the only symptoms. ...
... difficulty waking the baby and refusal to eat may be the only symptoms. ...
1960 THRESH, J. M. Quarantine arrangements for intercepting
... to a few leaves, perhaps those appearing only have been experienced within West Africa at certain stages of development. An ability through delays in transit and difficulty in reto detect such symptoms depends upon consid- establishing the sensitive material. Moreover, erable experience and even the ...
... to a few leaves, perhaps those appearing only have been experienced within West Africa at certain stages of development. An ability through delays in transit and difficulty in reto detect such symptoms depends upon consid- establishing the sensitive material. Moreover, erable experience and even the ...
Postinfection activity of selceted potato late blight fungicides
... low. Suppressing sporulation on potato foliage between tuber bulking and harvest is likely to decrease the number of tuber infections. Restricting lesion expansion also may be beneficial in reducing tuber infection since it would limit the total amount of plant tissue producing new spores. This stud ...
... low. Suppressing sporulation on potato foliage between tuber bulking and harvest is likely to decrease the number of tuber infections. Restricting lesion expansion also may be beneficial in reducing tuber infection since it would limit the total amount of plant tissue producing new spores. This stud ...
Clinical Application and Interpretation of Molecular Microbiological
... • False negatives due to presence of PCR inhibitors • Poor quality nucleic acid reduces sensitivity, e.g. formalin fixed tissue in paraffin blocks • False positives due to amplicon contaminants – especially with highly sensitive nested PCR. • Laboratory space, design and workflow needs to be careful ...
... • False negatives due to presence of PCR inhibitors • Poor quality nucleic acid reduces sensitivity, e.g. formalin fixed tissue in paraffin blocks • False positives due to amplicon contaminants – especially with highly sensitive nested PCR. • Laboratory space, design and workflow needs to be careful ...
Nepovirus Tomato black ring virus
... 1946). The virus was initially isolated from a ripe tomato fruit that showed lesions in the skin. The tomato was also infected with two other viruses, however, and TBRV alone was not linked to the fruit lesions. Despite the name, TBRV is rarely found in tomato, and tomato is not considered an econom ...
... 1946). The virus was initially isolated from a ripe tomato fruit that showed lesions in the skin. The tomato was also infected with two other viruses, however, and TBRV alone was not linked to the fruit lesions. Despite the name, TBRV is rarely found in tomato, and tomato is not considered an econom ...
Insects as Vectors of Disease Agents
... Vectors that increase their transmission rates over time have a latent period: the time between a vector’s acquisition of the pathogen and its first transmission to plants. Some vectors never transmit a pathogen until days or weeks after they first acquire it. In contrast to these long latent periods, ...
... Vectors that increase their transmission rates over time have a latent period: the time between a vector’s acquisition of the pathogen and its first transmission to plants. Some vectors never transmit a pathogen until days or weeks after they first acquire it. In contrast to these long latent periods, ...
Viral meningitis
... The typical viruses that cause viral meningitis can spread to close contacts, such as household members, friends and colleagues. The virus can be spread from just before an infected person gets sick; the person will then be infectious for about another week. Only some people who get infected will de ...
... The typical viruses that cause viral meningitis can spread to close contacts, such as household members, friends and colleagues. The virus can be spread from just before an infected person gets sick; the person will then be infectious for about another week. Only some people who get infected will de ...
1003 Association Between Number of Acute Retroviral Symptoms
... Background: Prompt identification of persons with acute HIV infection, particularly those likely to have high viral loads after acute HIV, presents important transmission prevention opportunities. In a large study of HIV-1 seroconverters across 9 sites in Africa, we sought to determine whether the n ...
... Background: Prompt identification of persons with acute HIV infection, particularly those likely to have high viral loads after acute HIV, presents important transmission prevention opportunities. In a large study of HIV-1 seroconverters across 9 sites in Africa, we sought to determine whether the n ...
Conventional and Molecular Detection of Infectious Bursal Disease
... chicken eggs were inoculated through chorio-allantoic membrane (CAM) at dose rate of 0.2ml of IBDV inoculum having titer of (EID50 105.50/100ul) (0.1 ml virus suspension + 0.1 ml antibiotic mixture). For IBDV samples of CAM were collected with PBS (phosphate buffered saline) to prepare 50% suspensio ...
... chicken eggs were inoculated through chorio-allantoic membrane (CAM) at dose rate of 0.2ml of IBDV inoculum having titer of (EID50 105.50/100ul) (0.1 ml virus suspension + 0.1 ml antibiotic mixture). For IBDV samples of CAM were collected with PBS (phosphate buffered saline) to prepare 50% suspensio ...
Current Trends in the Diagnosis of Equine Infectious Diseases
... mosquito cells, and indirect immunofluorescence using a monoclonal antibody (MAb) of high specificity is necessary to confirm the presence of and / or to identify virus isolates. Alternatively, the presence of the virus can be confirmed by nucleic acid detection. A sensitive and WNVspecific reverse ...
... mosquito cells, and indirect immunofluorescence using a monoclonal antibody (MAb) of high specificity is necessary to confirm the presence of and / or to identify virus isolates. Alternatively, the presence of the virus can be confirmed by nucleic acid detection. A sensitive and WNVspecific reverse ...
Detect Dengue-Zika White Paper
... DENV, CHIKV, and ZIKV can be diagnosed through viral isolation, serological tests, molecular-based methods, or a combination of these techniques13-15. Viral isolation enables the identification of an active infection and is considered to be a sensitive and specific form of detection. However, this m ...
... DENV, CHIKV, and ZIKV can be diagnosed through viral isolation, serological tests, molecular-based methods, or a combination of these techniques13-15. Viral isolation enables the identification of an active infection and is considered to be a sensitive and specific form of detection. However, this m ...
Isolation of Clostridium tetani and detection of tetanus
... units). On August 18, generalized convulsions, trismus, and spastic paralysis of the upper extremities and trunk appeared. Typical Gram-positive drumstick-shaped bacilli were detected in samples from the injury site. The administration of penicillin G (20,000,000 units/day) was started together with ...
... units). On August 18, generalized convulsions, trismus, and spastic paralysis of the upper extremities and trunk appeared. Typical Gram-positive drumstick-shaped bacilli were detected in samples from the injury site. The administration of penicillin G (20,000,000 units/day) was started together with ...
Penaeus monodon by RT-PCR amplification
... The purpose of this work was to develop a specific and sensitive RT-PCR for the detection of YHV. If successful, the detection of YHV RNA by this process would have advantages over direct dot blot nucleic acid hybridization tests in terms of a higher sensitivity and a shorter detection time. However ...
... The purpose of this work was to develop a specific and sensitive RT-PCR for the detection of YHV. If successful, the detection of YHV RNA by this process would have advantages over direct dot blot nucleic acid hybridization tests in terms of a higher sensitivity and a shorter detection time. However ...
HCV PCR
... catalyzes and destroys all the DNA containing deoxyuridine and also removes nonspecific product formed after initial activation of the mastermix by Mn2+ – Naturally occuring DNA does not contain deoxyuridine – Deoxyuridine is in master mix and DNA containing it is removed before amplification ...
... catalyzes and destroys all the DNA containing deoxyuridine and also removes nonspecific product formed after initial activation of the mastermix by Mn2+ – Naturally occuring DNA does not contain deoxyuridine – Deoxyuridine is in master mix and DNA containing it is removed before amplification ...
[BIO24] Detection of infectious bursal disease virus using SYBR
... detect vaccine and wild type IBDV strains in infected chickens. Although, these method was able to differentiate different IBDV strains, they are not preferred to be tested on large number of clinical samples. The present study, offers an alternative format assay in detecting and differentiate of ve ...
... detect vaccine and wild type IBDV strains in infected chickens. Although, these method was able to differentiate different IBDV strains, they are not preferred to be tested on large number of clinical samples. The present study, offers an alternative format assay in detecting and differentiate of ve ...
Banana bunchy top virus
... Biology and ecology: BBTV is transmitted by an aphid vector (Pentalonia nigronervosa) and is disseminated in vegetative planting material, but is not transmitted by mechanical inoculation (Magee, 1927). Distribution and Movement within the Plant Magee (1927) showed that banana bunchy top was system ...
... Biology and ecology: BBTV is transmitted by an aphid vector (Pentalonia nigronervosa) and is disseminated in vegetative planting material, but is not transmitted by mechanical inoculation (Magee, 1927). Distribution and Movement within the Plant Magee (1927) showed that banana bunchy top was system ...
Plant Plus Strand RNA Viruses
... • Long distance movement in plant – through phloem – as particles or protein/nucleic acid complex (coat protein required) ...
... • Long distance movement in plant – through phloem – as particles or protein/nucleic acid complex (coat protein required) ...
Seminars in Cell & Developmental Biology Hsiao-Yun Yeh , Marylynn V. Yates
... shown that in situ RT-PCR (in situ PCR) allows for the detection of RNA sequences of infectious bursal disease virus and human papillomavirus DNA with copy numbers below the detection threshold of conventional ISH analysis [19,20]. DNA microarray has become an alternate hybridization method for the ...
... shown that in situ RT-PCR (in situ PCR) allows for the detection of RNA sequences of infectious bursal disease virus and human papillomavirus DNA with copy numbers below the detection threshold of conventional ISH analysis [19,20]. DNA microarray has become an alternate hybridization method for the ...
Advanced Detection of Viruses and Protozoan Parasites in Water
... PCR assay for viruses differ significantly in several ways. For cell culture, the minimum detection level of viruses in a sample is, by definition, one PFU per unit volume - a quantity of virus particles that may range from just a few or many more -at least some of which must be infectious. In addit ...
... PCR assay for viruses differ significantly in several ways. For cell culture, the minimum detection level of viruses in a sample is, by definition, one PFU per unit volume - a quantity of virus particles that may range from just a few or many more -at least some of which must be infectious. In addit ...
Who`s Got the Flu? - Cornell Center for Materials Research
... help us fight off these, possibly, deadly pathogens. Our second line of defense, also called active immunity, is a process in which are our body produces antibodies that identifies a certain pathogen. Once identified, a cascade of reactions occurs to stimulate the production of white blood cells to ...
... help us fight off these, possibly, deadly pathogens. Our second line of defense, also called active immunity, is a process in which are our body produces antibodies that identifies a certain pathogen. Once identified, a cascade of reactions occurs to stimulate the production of white blood cells to ...
Evaluation of detection methods for Virus, Viroids and
... Phytoplasma detection by PCR: For phytoplasma detection by PCR, samples were collected between October and December, which has been shown to be the best period for the detection in our climate (Garcia et al. 2003). DNA was isolated from approximately 1.0 g of fresh stems, using the phytoplasma-enric ...
... Phytoplasma detection by PCR: For phytoplasma detection by PCR, samples were collected between October and December, which has been shown to be the best period for the detection in our climate (Garcia et al. 2003). DNA was isolated from approximately 1.0 g of fresh stems, using the phytoplasma-enric ...
Potato virus Y
Potato virus Y (PVY) is a plant pathogenic virus of the family Potyviridae, and one of the most important plant viruses affecting potato production.PVY infection of potato plants results in a variety of symptoms depending on the viral strain. The mildest of these symptoms is production loss, but the most detrimental is 'potato tuber necrotic ringspot disease' (PTNRD). Necrotic ringspots render potatoes unmarketable and can therefore result in a significant loss of income. PVY is transmissible by aphid vectors but may also remain dormant in seed potatoes. This means that using the same line of potato for production of seed potatoes for several consecutive generations will lead to a progressive increase in viral load and subsequent loss of crop.An increase in potato plant infection with viruses over the past few years has led to considerable losses to the South African potato industry. The increased rate of infection may be attributed to several factors. These include a marked decrease in the effectiveness and administration of chemicals used in vector control, the use of infected seed potatoes in cultivation, incorrect irrigation and farming methods as well as a lack of a sensitive, rapid and reliable method of detection. An increase in the average temperature of winters as a consequence of global warming has also led to an increase in aphid numbers, which in turn has led to an increase in viral distribution.