A novel plant virus with unique properties infecting Japanese holly fern
... (Fig. 2a). dsRNA profiles of JHFMoV-JM, DI and HR were indistinguishable (Fig. 2b), indicating the presence of the same virus in all three specimens. Similar dsRNA profiles were obtained from all diseased JHF plants of different geographical origins, supporting their association with the symptoms as ...
... (Fig. 2a). dsRNA profiles of JHFMoV-JM, DI and HR were indistinguishable (Fig. 2b), indicating the presence of the same virus in all three specimens. Similar dsRNA profiles were obtained from all diseased JHF plants of different geographical origins, supporting their association with the symptoms as ...
Infectious Hematopoietic Necrosis Virus
... Virus Infectious Hematopoietic Necrosis Virus (IHNV) is an RNA virus of the Novirhabdovirus genus which causes Infectious Hematopoietic Necrosis (IHN), a chronic disease of Salmonoid fish. The linear, single-stranded, negative-sense RNA genome of this virus 11,131 nucleotides long and encodes six ge ...
... Virus Infectious Hematopoietic Necrosis Virus (IHNV) is an RNA virus of the Novirhabdovirus genus which causes Infectious Hematopoietic Necrosis (IHN), a chronic disease of Salmonoid fish. The linear, single-stranded, negative-sense RNA genome of this virus 11,131 nucleotides long and encodes six ge ...
Syncytial Hepatitis of Tilapia - Columbia University Mailman School
... electron-dense coating, suggestive of virion endocytosis. We suspect that the process noted is similar to that previously observed in other orthomyxoviruses, where both clathrindependent and clathrin-independent endocytic mechanisms may be involved.17 However, more work is required to ascertain this ...
... electron-dense coating, suggestive of virion endocytosis. We suspect that the process noted is similar to that previously observed in other orthomyxoviruses, where both clathrindependent and clathrin-independent endocytic mechanisms may be involved.17 However, more work is required to ascertain this ...
Detecting Antibodies against Infectious Bronchitis Virus Using an
... binding, we used dilution buffer in final NaCl concentration of 0.15M however, higher concentrations of NaCl was not tested. The experimental ELISA was detected low to moderate level after live vaccination and moderate to relatively high levels of antibody against IBV after injection of inactivated ...
... binding, we used dilution buffer in final NaCl concentration of 0.15M however, higher concentrations of NaCl was not tested. The experimental ELISA was detected low to moderate level after live vaccination and moderate to relatively high levels of antibody against IBV after injection of inactivated ...
01_front. - Massey Research Online
... specificity of 73 .68% for detecting the New Zealand IBDV strain infection. This indicated that there should be further evaluation of the use of serological testing as the sole method for the detection of IBDV infected farms in the current control scheme. ...
... specificity of 73 .68% for detecting the New Zealand IBDV strain infection. This indicated that there should be further evaluation of the use of serological testing as the sole method for the detection of IBDV infected farms in the current control scheme. ...
Isolation and identification of Peste des Petits Ruminants Virus
... Enzyme Linked Immunosorbent Assay (IC ELISA). The IC ELISA showed presence of PPR antigen 43.3% (13/30) in buccal mucosa samples, 36.6% (11/30) in nasal discharges, 40% (12/30) in ocular discharges and 80% (24/30) in lymph nodes. Overall percentage of PPR antigen in all different samples detected by ...
... Enzyme Linked Immunosorbent Assay (IC ELISA). The IC ELISA showed presence of PPR antigen 43.3% (13/30) in buccal mucosa samples, 36.6% (11/30) in nasal discharges, 40% (12/30) in ocular discharges and 80% (24/30) in lymph nodes. Overall percentage of PPR antigen in all different samples detected by ...
FAO Collaborative Studies for FMD Standardisation: Phase XIX - Virological Assays
... the SVD virus samples (UKG 76/74), one NVD sample (no virus detected; IRN 19/2003) and three 10-fold dilution steps from the Asia 1 PAK 20/2003 titration series. Thirty identical sets of 20 samples of 5 ml volumes were prepared and each sample was uniquely coded to ensure ‘blind’ testing and labelle ...
... the SVD virus samples (UKG 76/74), one NVD sample (no virus detected; IRN 19/2003) and three 10-fold dilution steps from the Asia 1 PAK 20/2003 titration series. Thirty identical sets of 20 samples of 5 ml volumes were prepared and each sample was uniquely coded to ensure ‘blind’ testing and labelle ...
QuickTiter™ Hepatitis B Surface Antigen (HBsAg) ELISA Kit
... 3. Incubate 30 minutes at 37ºC. Note: For samples containing anti-HBsAg antibodies, inactivate by incubating at 56ºC for 30 min. ...
... 3. Incubate 30 minutes at 37ºC. Note: For samples containing anti-HBsAg antibodies, inactivate by incubating at 56ºC for 30 min. ...
QuickTiter™ Hepatitis B Surface Antigen (HBsAg) ELISA Kit
... 3. Incubate 30 minutes at 37ºC. Note: For samples containing anti-HBsAg antibodies, inactivate by incubating at 56ºC for 30 min. ...
... 3. Incubate 30 minutes at 37ºC. Note: For samples containing anti-HBsAg antibodies, inactivate by incubating at 56ºC for 30 min. ...
Article (Published version)
... imens (n p 27) varied from below the level of quantitative detection to 102 copies/200 ng of total DNA (data not shown). Spleens from 3 (75%) of 4 donor pigs were positive for PLHV-1 by conventional and quantitative PCR (range, 1.9 ⫻ 101–1.47 ⫻ 103/mg of total DNA); 1 spleen (25%) was also positive ...
... imens (n p 27) varied from below the level of quantitative detection to 102 copies/200 ng of total DNA (data not shown). Spleens from 3 (75%) of 4 donor pigs were positive for PLHV-1 by conventional and quantitative PCR (range, 1.9 ⫻ 101–1.47 ⫻ 103/mg of total DNA); 1 spleen (25%) was also positive ...
CBP's Powerpoint template for scientific posters
... The regions of the MD2004 genome which differ the most from MD-145 and other pre-2002 strains of norovirus are the ORF2 and ORF3. Variations in the ORF2 region might be expected since it encodes the capsid protein which is presumably under selective immune pressure [2]. in addition to several amino ...
... The regions of the MD2004 genome which differ the most from MD-145 and other pre-2002 strains of norovirus are the ORF2 and ORF3. Variations in the ORF2 region might be expected since it encodes the capsid protein which is presumably under selective immune pressure [2]. in addition to several amino ...
A sandwich ELISA to detect VHSV and IPNV in turbot | SpringerLink
... Thereafter, plates were dried for 2 h at 37 “C and sealed with silica gel at 4 “C. Plates coated with mouse IgCl (to control Mab 2D5) or IgG2a (to control Mab 2Al) (Chemicon Int., Temecula, CA, USA; 1 ~1 IgCl or IgCi2adiluted in 100 ~1 of distilled water: volume per well 100 ~1; 1.12 ~1 2Al diluted ...
... Thereafter, plates were dried for 2 h at 37 “C and sealed with silica gel at 4 “C. Plates coated with mouse IgCl (to control Mab 2D5) or IgG2a (to control Mab 2Al) (Chemicon Int., Temecula, CA, USA; 1 ~1 IgCl or IgCi2adiluted in 100 ~1 of distilled water: volume per well 100 ~1; 1.12 ~1 2Al diluted ...
Infectious salmon anaemia virus
... The CT value obtained with the internal control will vary significantly depending on the extraction efficiency, the quantity of RNA added to the RT and PCR reaction and the individual machine settings. CT values of 28±3 are within the normal range. When amplifying a ISAV sample with a high genome co ...
... The CT value obtained with the internal control will vary significantly depending on the extraction efficiency, the quantity of RNA added to the RT and PCR reaction and the individual machine settings. CT values of 28±3 are within the normal range. When amplifying a ISAV sample with a high genome co ...
Nat. Commun. v1 SFE130107
... retroelements10-12. Sheehy et al. demonstrated that A3G also plays a role in immunity against Human immunodeficiency virus type 1 (HIV-1)13. For its antiviral role A3G is packaged along with viral RNA14. Upon infection of target cells and during the reverse transcription process, A3G deaminates the ...
... retroelements10-12. Sheehy et al. demonstrated that A3G also plays a role in immunity against Human immunodeficiency virus type 1 (HIV-1)13. For its antiviral role A3G is packaged along with viral RNA14. Upon infection of target cells and during the reverse transcription process, A3G deaminates the ...
isolation, identification and antibiogram pattern of
... accordance with Sameera et al., 2001. These tests ruled out the possibilities of infection caused by Pasteurella, Salmonella or Escherichia coli species. The primer combination used in this study was reliable and very specific in amplifying 500 bp fragment of 16s rRNA region of A. paragallinarum as ...
... accordance with Sameera et al., 2001. These tests ruled out the possibilities of infection caused by Pasteurella, Salmonella or Escherichia coli species. The primer combination used in this study was reliable and very specific in amplifying 500 bp fragment of 16s rRNA region of A. paragallinarum as ...
Today, 4/4/00 (If you`re out of your seat w/o permission, you will get
... Pack/Wrap-up (last few mins of class) ...
... Pack/Wrap-up (last few mins of class) ...
... multiplexes RT-PCR for AI, ND and IBD viruses described in present work. Early detection and differential diagnosis of AI, ND and IBD is necessary for the control of these viral diseases. The PCR has applied to the rapid detection and differential diagnosis of AI, ND and IBD. It resulted in a very s ...
This article was published in an Elsevier journal. The attached copy
... a result, genome-sized viral DNA is formed, spreads systemically through the plant and induces disease symptoms. Agroinoculation has been used successfully to introduce cloned tandem repeats of TYLCV DNA into leaf disks as well as into whole plants (Czosnek et al., 1993; Kheyr-Pour et al., 1994). Ho ...
... a result, genome-sized viral DNA is formed, spreads systemically through the plant and induces disease symptoms. Agroinoculation has been used successfully to introduce cloned tandem repeats of TYLCV DNA into leaf disks as well as into whole plants (Czosnek et al., 1993; Kheyr-Pour et al., 1994). Ho ...
mild mosaic and faint mottle ringspot, two papaya virus diseases of
... Aphid Transmission Tests. Repeated attempts to transmit MMV with several species of aphids were unsuccessful. The procedures used to demon strate aphid transmission of the distortion ring- ...
... Aphid Transmission Tests. Repeated attempts to transmit MMV with several species of aphids were unsuccessful. The procedures used to demon strate aphid transmission of the distortion ring- ...
QuickTiter™ Hepatitis B Surface Antigen (HBsAg
... including the blank wells. Incubate at room temperature on an orbital shaker. Actual incubation time may vary from 5-20 minutes. Note: Watch plate carefully; if color changes rapidly, the reaction may need to be stopped sooner to prevent saturation. 13. Stop the enzyme reaction by adding 100 µL of S ...
... including the blank wells. Incubate at room temperature on an orbital shaker. Actual incubation time may vary from 5-20 minutes. Note: Watch plate carefully; if color changes rapidly, the reaction may need to be stopped sooner to prevent saturation. 13. Stop the enzyme reaction by adding 100 µL of S ...
RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES
... Leptospirosis is a zoonotic disease with a world wide distribution. It has been underdiagnosed and under-reported in India due to lack of awareness of the disease, inadequate epidemiological data and unavailability of appropriate laboratory diagnostic facilities.1 The disease symptoms range from a m ...
... Leptospirosis is a zoonotic disease with a world wide distribution. It has been underdiagnosed and under-reported in India due to lack of awareness of the disease, inadequate epidemiological data and unavailability of appropriate laboratory diagnostic facilities.1 The disease symptoms range from a m ...
Infectious Hematopoietic Necrosis Virus
... Virus Infectious Hematopoietic Necrosis Virus (IHNV) is an RNA virus of the Novirhabdovirus genus which causes Infectious Hematopoietic Necrosis (IHN), a chronic disease of Salmonoid fish. The linear, single-stranded, negative-sense RNA genome of this virus 11,131 nucleotides long and encodes six ge ...
... Virus Infectious Hematopoietic Necrosis Virus (IHNV) is an RNA virus of the Novirhabdovirus genus which causes Infectious Hematopoietic Necrosis (IHN), a chronic disease of Salmonoid fish. The linear, single-stranded, negative-sense RNA genome of this virus 11,131 nucleotides long and encodes six ge ...
Infectious salmon anemia virus (HPR0, avirulent)
... The CT value obtained with the internal control will vary significantly depending on the extraction efficiency, the quantity of RNA added to the RT and PCR reaction and the individual machine settings. CT values of 28±3 are within the normal range. When amplifying a ISAV-HPR0 sample with a high geno ...
... The CT value obtained with the internal control will vary significantly depending on the extraction efficiency, the quantity of RNA added to the RT and PCR reaction and the individual machine settings. CT values of 28±3 are within the normal range. When amplifying a ISAV-HPR0 sample with a high geno ...
IJBT 4(2) 284-286
... in terms of incubation period, pathological and clinical effects. In contrast, the virus isolated from naturally infected animals is termed as street virus. The street virus may have incubation periods varying from 2 weeks to more than 1 year and variable clinical effects2. The vaccines available to ...
... in terms of incubation period, pathological and clinical effects. In contrast, the virus isolated from naturally infected animals is termed as street virus. The street virus may have incubation periods varying from 2 weeks to more than 1 year and variable clinical effects2. The vaccines available to ...
Potato virus Y
Potato virus Y (PVY) is a plant pathogenic virus of the family Potyviridae, and one of the most important plant viruses affecting potato production.PVY infection of potato plants results in a variety of symptoms depending on the viral strain. The mildest of these symptoms is production loss, but the most detrimental is 'potato tuber necrotic ringspot disease' (PTNRD). Necrotic ringspots render potatoes unmarketable and can therefore result in a significant loss of income. PVY is transmissible by aphid vectors but may also remain dormant in seed potatoes. This means that using the same line of potato for production of seed potatoes for several consecutive generations will lead to a progressive increase in viral load and subsequent loss of crop.An increase in potato plant infection with viruses over the past few years has led to considerable losses to the South African potato industry. The increased rate of infection may be attributed to several factors. These include a marked decrease in the effectiveness and administration of chemicals used in vector control, the use of infected seed potatoes in cultivation, incorrect irrigation and farming methods as well as a lack of a sensitive, rapid and reliable method of detection. An increase in the average temperature of winters as a consequence of global warming has also led to an increase in aphid numbers, which in turn has led to an increase in viral distribution.