ibv_2_search - Cairo University Scholars
... 9. Reagents for histopathology according to (Bancroft and Steven, 1977) 10. Cross protection test: To evaluate the protection of the respiratory tract provided by live-attenuated IB vaccine against challenge with IBV isolates that proved to be variant by sequencing. Seventy one day old specific path ...
... 9. Reagents for histopathology according to (Bancroft and Steven, 1977) 10. Cross protection test: To evaluate the protection of the respiratory tract provided by live-attenuated IB vaccine against challenge with IBV isolates that proved to be variant by sequencing. Seventy one day old specific path ...
Fungal viruses, hypovirulence, and biological control of Sclerotinia
... Abstract: Hypovirulence in fungal plant pathogens refers to the reduced ability of selected isolates within a population of a pathogen to infect, colonize, kill, and (or) reproduce on susceptible host tissues and is often associated with fungal viruses and associated double-stranded RNA elements. It ...
... Abstract: Hypovirulence in fungal plant pathogens refers to the reduced ability of selected isolates within a population of a pathogen to infect, colonize, kill, and (or) reproduce on susceptible host tissues and is often associated with fungal viruses and associated double-stranded RNA elements. It ...
A Current Overview of Two Viroids That Infect
... In Akita Prefecture, Japan, CChMVd infections in chrysanthemums caused distinct yellow leaf mottling and necrosis. These symptoms were caused by CChMVd variants containing a UUUC tetraloop (Figure 1b) [50], a structural domain already identified as a specific CChMVd pathogenic determinant [26]. A pr ...
... In Akita Prefecture, Japan, CChMVd infections in chrysanthemums caused distinct yellow leaf mottling and necrosis. These symptoms were caused by CChMVd variants containing a UUUC tetraloop (Figure 1b) [50], a structural domain already identified as a specific CChMVd pathogenic determinant [26]. A pr ...
Chapter 2.2.3.
... 2.2.4. Target organs and infected tissue IHHNV infects and has been shown to replicate (using in situ hybridisation [ISH] with specific DNA probes) in tissues of ectodermal and mesodermal origin from the embryo. Thus, the principal target organs include: the gills, cuticular epithelium (or hypodermi ...
... 2.2.4. Target organs and infected tissue IHHNV infects and has been shown to replicate (using in situ hybridisation [ISH] with specific DNA probes) in tissues of ectodermal and mesodermal origin from the embryo. Thus, the principal target organs include: the gills, cuticular epithelium (or hypodermi ...
Laboratory Manual for the Diagnosis of Whooping
... ADP-ribosylating activity. This toxin is not expressed by B. parapertussis. The toxin adenylate cyclase-haemolysin (AC-Hly) is a secreted trifunctional protein. It expresses a calmodulin-dependent adenylate cyclase activity, a haemolytic activity and an invasive activity. This toxin binds the integr ...
... ADP-ribosylating activity. This toxin is not expressed by B. parapertussis. The toxin adenylate cyclase-haemolysin (AC-Hly) is a secreted trifunctional protein. It expresses a calmodulin-dependent adenylate cyclase activity, a haemolytic activity and an invasive activity. This toxin binds the integr ...
Table of Published Studies on XMRV and pMLV Findings in Human Diseases and the General Population
... midwestern United States. Retrovirology 2011; 8:23. doi:10.1186/1742-4690-8-23. ...
... midwestern United States. Retrovirology 2011; 8:23. doi:10.1186/1742-4690-8-23. ...
Lowering the Detection Limits of HIV-1 Viral Load Using Real
... coupled to the detection of HIV-1 p24 antigen in the IPCR. The PCR standard curve (using only template DNA) displayed a correlation coefficient of 0.997, and 1 to 10 copies of DNA were able to be detected (data not shown). To generate a standard curve correlating with HIV-1 p24 antigen quantificatio ...
... coupled to the detection of HIV-1 p24 antigen in the IPCR. The PCR standard curve (using only template DNA) displayed a correlation coefficient of 0.997, and 1 to 10 copies of DNA were able to be detected (data not shown). To generate a standard curve correlating with HIV-1 p24 antigen quantificatio ...
Elisabeth Pharmacon 2014 CE HBV and HCV
... pneumoniae is associated with a remarkably wide range of chronic diseases as meningoencephalitis, arthritis or myocarditis and it is the second most often cause of community-acquired pneumonia. C. pneumoniae spreads by drop infection but in the form of infection elementary bodies (EB) resistant to e ...
... pneumoniae is associated with a remarkably wide range of chronic diseases as meningoencephalitis, arthritis or myocarditis and it is the second most often cause of community-acquired pneumonia. C. pneumoniae spreads by drop infection but in the form of infection elementary bodies (EB) resistant to e ...
Full text in pdf format
... RT-PCR and nested RT-PCR for Mr NV and XSV. RTPCR was carried out using the Reverse-IT TM 1-step RTPCR kit (ABgene), allowing reverse transcription (RT) and amplification to be performed in a single reaction tube. Nested RT-PCR was carried out using the products of RT-PCR wherever it was necessary t ...
... RT-PCR and nested RT-PCR for Mr NV and XSV. RTPCR was carried out using the Reverse-IT TM 1-step RTPCR kit (ABgene), allowing reverse transcription (RT) and amplification to be performed in a single reaction tube. Nested RT-PCR was carried out using the products of RT-PCR wherever it was necessary t ...
Yellow leaf of sugarcane is caused by at least three different
... into the lamina, although this symptom may be related to other biotic or abiotic factors [12]. SCYLV can cause important yield losses in cultivars susceptible to yellow leaf, even if infected plants do not exhibit overt disease symptoms [10, 20, 28]. The virus is transmitted by the aphids Melanaphis ...
... into the lamina, although this symptom may be related to other biotic or abiotic factors [12]. SCYLV can cause important yield losses in cultivars susceptible to yellow leaf, even if infected plants do not exhibit overt disease symptoms [10, 20, 28]. The virus is transmitted by the aphids Melanaphis ...
CHAPTER 4 The editorial style of Water Research 4
... epidemiological data which associate substantial levels of enteric infections with drinking water supplies which meet all specifications for treatment, disinfection and counts of indicator organisms (Rose et aL, 1986; Bosch et aL, 1991; Payment et aL, 1997). ...
... epidemiological data which associate substantial levels of enteric infections with drinking water supplies which meet all specifications for treatment, disinfection and counts of indicator organisms (Rose et aL, 1986; Bosch et aL, 1991; Payment et aL, 1997). ...
Structure resolution of the trimeric RNA
... The viral replication is taking place in two steps. The RNA segment of each vRNP is first copied in complementary cRNA strand of positive polarity, which forms cRNP after the loading of NP oligomers and the association of a polymerase complex. Each cRNP serves as template for the synthesis of vRNA s ...
... The viral replication is taking place in two steps. The RNA segment of each vRNP is first copied in complementary cRNA strand of positive polarity, which forms cRNP after the loading of NP oligomers and the association of a polymerase complex. Each cRNP serves as template for the synthesis of vRNA s ...
Mixed infection with multiple strains of murine cytomegalovirus
... As with human cytomegalovirus (HCMV) infection of humans, murine CMV (MCMV) infection is widespread in its natural host, the house mouse Mus domesticus, and may consist of mixed infection with different CMV isolates. The incidence and mechanisms by which mixed infection occurs in free-living mice ar ...
... As with human cytomegalovirus (HCMV) infection of humans, murine CMV (MCMV) infection is widespread in its natural host, the house mouse Mus domesticus, and may consist of mixed infection with different CMV isolates. The incidence and mechanisms by which mixed infection occurs in free-living mice ar ...
Genetic sequencing and analysis of the infectious pancreatic
... 3.5 Sequence analysis.............................................................................................. 13 3.6 Phylogenetic analysis........................................................................................ 14 ...
... 3.5 Sequence analysis.............................................................................................. 13 3.6 Phylogenetic analysis........................................................................................ 14 ...
A Partial Host Range of the High Plains Virus of Corn and Wheat
... Plains virus (HPV) in greenhouse experiments, but several other grass species were not. Infection of a host was dependent upon wheat curl mite numbers. Although both green foxtail (Setaria viridis) and yellow foxtail (S. glauca) are found naturally infected by HPV, only yellow foxtail could be infec ...
... Plains virus (HPV) in greenhouse experiments, but several other grass species were not. Infection of a host was dependent upon wheat curl mite numbers. Although both green foxtail (Setaria viridis) and yellow foxtail (S. glauca) are found naturally infected by HPV, only yellow foxtail could be infec ...
Large differences between test strategies for the detection of anti
... Current guidelines for the diagnosis of Lyme disease include a two-tier testing algorithm [2,3]. First an ELISA is performed, followed by confirmation of positive ELISA results with an immunoblot. This two-step procedure was initiated because first generation ELISA‟s for the detection of anti-Borrel ...
... Current guidelines for the diagnosis of Lyme disease include a two-tier testing algorithm [2,3]. First an ELISA is performed, followed by confirmation of positive ELISA results with an immunoblot. This two-step procedure was initiated because first generation ELISA‟s for the detection of anti-Borrel ...
- Mediterranean Journal of Hematology and Infectious
... Serum and breast milk became negative for HBsAg 6 months after delivery without detection ...
... Serum and breast milk became negative for HBsAg 6 months after delivery without detection ...
Nucleotide sequence and genome structure of grapevine rupestris
... and the limited quantity of RSP-infected grapevine materials precluded the use of a single RSP-infected grapevine accession as the source of dsRNA for cloning purposes. Therefore, dsRNA preparations from Colobel 257, Ravat 34, Couderc 28-112 and Seyval were pooled and used as templates for cDNA synt ...
... and the limited quantity of RSP-infected grapevine materials precluded the use of a single RSP-infected grapevine accession as the source of dsRNA for cloning purposes. Therefore, dsRNA preparations from Colobel 257, Ravat 34, Couderc 28-112 and Seyval were pooled and used as templates for cDNA synt ...
View PDF - cchem.berkeley.edu - University of California, Berkeley
... may cause adverse immune responses, a separate inactivation step must complement downstream separation processes. The most commonly used helper inactivation process is heat treatment (typically at 568C for 1 h), but such an incubation has been reported to cause a concomitant inactivation of 50% of ...
... may cause adverse immune responses, a separate inactivation step must complement downstream separation processes. The most commonly used helper inactivation process is heat treatment (typically at 568C for 1 h), but such an incubation has been reported to cause a concomitant inactivation of 50% of ...
Diagnosis of genital herpes simplex virus infection in the clinical laboratory
... Viral culture with further herpes typing has been the cornerstone of HSV diagnosis over the past two decades and accepted as the gold standard for the laboratory diagnosis of HSV infections. Viral antigen can be easily detected by direct immunofluorescence (IF) assay using fluorescein-labelled types ...
... Viral culture with further herpes typing has been the cornerstone of HSV diagnosis over the past two decades and accepted as the gold standard for the laboratory diagnosis of HSV infections. Viral antigen can be easily detected by direct immunofluorescence (IF) assay using fluorescein-labelled types ...
In Situ Capture RT-qPCR: A New Simple and Sensitive Method to
... are the most commonly-used methods for detecting HuNoVs (Fisman et al., 2009; Knight et al., 2013). Recently, RT-PCR assays have been largely replaced by the use of one-step RT-qPCR which allows for signal amplification and amplicon confirmation in a single reaction (Knight et al., 2013). RT-PCR has ...
... are the most commonly-used methods for detecting HuNoVs (Fisman et al., 2009; Knight et al., 2013). Recently, RT-PCR assays have been largely replaced by the use of one-step RT-qPCR which allows for signal amplification and amplicon confirmation in a single reaction (Knight et al., 2013). RT-PCR has ...
Detection of bacteria, fungi, and viruses by a real
... blood culture remains low, and also it takes time to ...
... blood culture remains low, and also it takes time to ...
Plant Disease
... against viral infection has been documented using biological and chemical inducing agents (1,2,7,10,12,16,21,23). In most cases, the biological agents consisted of plant pathogenic bacteria, fungi, or viruses. An alternative method to induce plant defenses is through the use of nonpathogenic microor ...
... against viral infection has been documented using biological and chemical inducing agents (1,2,7,10,12,16,21,23). In most cases, the biological agents consisted of plant pathogenic bacteria, fungi, or viruses. An alternative method to induce plant defenses is through the use of nonpathogenic microor ...
Sore throat in primary care project: a clinical score
... could make a major difference in the clinical approach of many family physicians and other doctors. The first variable of the score is absence of headache. It has been reported that although headache is not one of the Centor criteria, it is a commonly looked for symptom of strep throat and is associ ...
... could make a major difference in the clinical approach of many family physicians and other doctors. The first variable of the score is absence of headache. It has been reported that although headache is not one of the Centor criteria, it is a commonly looked for symptom of strep throat and is associ ...
Specificity of a Polymerase Chain Reaction Assay of a Target
... pellet was redissolved in 0.1 ml of sterile Milli-Q water and rehydrated overnight at room temperature. ...
... pellet was redissolved in 0.1 ml of sterile Milli-Q water and rehydrated overnight at room temperature. ...
Potato virus Y
Potato virus Y (PVY) is a plant pathogenic virus of the family Potyviridae, and one of the most important plant viruses affecting potato production.PVY infection of potato plants results in a variety of symptoms depending on the viral strain. The mildest of these symptoms is production loss, but the most detrimental is 'potato tuber necrotic ringspot disease' (PTNRD). Necrotic ringspots render potatoes unmarketable and can therefore result in a significant loss of income. PVY is transmissible by aphid vectors but may also remain dormant in seed potatoes. This means that using the same line of potato for production of seed potatoes for several consecutive generations will lead to a progressive increase in viral load and subsequent loss of crop.An increase in potato plant infection with viruses over the past few years has led to considerable losses to the South African potato industry. The increased rate of infection may be attributed to several factors. These include a marked decrease in the effectiveness and administration of chemicals used in vector control, the use of infected seed potatoes in cultivation, incorrect irrigation and farming methods as well as a lack of a sensitive, rapid and reliable method of detection. An increase in the average temperature of winters as a consequence of global warming has also led to an increase in aphid numbers, which in turn has led to an increase in viral distribution.