Linköping University Post Print An unbiased metagenomic search for infectious
... individual was kept in these analyses as this is conservative and conforms to our prior intentions. GBV-C (also known as hepatitis G virus) is an RNA virus and member of the Flaviviridae family with greatest homology to hepatitis C virus. It is transmitted via multiple modalities (e.g., vertically, ...
... individual was kept in these analyses as this is conservative and conforms to our prior intentions. GBV-C (also known as hepatitis G virus) is an RNA virus and member of the Flaviviridae family with greatest homology to hepatitis C virus. It is transmitted via multiple modalities (e.g., vertically, ...
Methods for Detection of Viruses in Water and Wastewater
... assay is a test where ligand binds covalently to peroxidase. During the test ligand binds with antibody of interest. Unbound ligand is washed out and bound ligand in examined by addition of substrate, which are converted into colored products, as a result of enzyme activity [47]. Very important agen ...
... assay is a test where ligand binds covalently to peroxidase. During the test ligand binds with antibody of interest. Unbound ligand is washed out and bound ligand in examined by addition of substrate, which are converted into colored products, as a result of enzyme activity [47]. Very important agen ...
Detection of yellow fever virus: a comparison of
... Yellow fever virus quantitation is performed routinely by cultivation of virus containing samples using susceptible cells. Counting of the resulting plaques provides a marker for the number of infectious particles present in the sample. This assay usually takes up to 5 days before results are obtain ...
... Yellow fever virus quantitation is performed routinely by cultivation of virus containing samples using susceptible cells. Counting of the resulting plaques provides a marker for the number of infectious particles present in the sample. This assay usually takes up to 5 days before results are obtain ...
Real time RT-PCR for SFTS virus
... In this study, the specimens were evaluated co-infection by Malaria and SFTS. In the previous study we evaluated dengue diagnosis by three assay (RT-PCR, IgM and NS1 antigen ELISA) (data not shown). Among 456 specimens, 22 showed malaria positive (4.8%) and SFTS virus was detected from the onl ...
... In this study, the specimens were evaluated co-infection by Malaria and SFTS. In the previous study we evaluated dengue diagnosis by three assay (RT-PCR, IgM and NS1 antigen ELISA) (data not shown). Among 456 specimens, 22 showed malaria positive (4.8%) and SFTS virus was detected from the onl ...
et al.
... produced, are the most commonly related with food-borne viral outbreaks (Pintó et al., 2009). Shellfish have been considered as a major source of food-borne viruses due to their filter-feeding mechanism that can concentrate virus from polluted waters (Carter, 2005). Pork meat products are also a sig ...
... produced, are the most commonly related with food-borne viral outbreaks (Pintó et al., 2009). Shellfish have been considered as a major source of food-borne viruses due to their filter-feeding mechanism that can concentrate virus from polluted waters (Carter, 2005). Pork meat products are also a sig ...
viral hemorrhagic fevers - the County of Santa Clara
... Many viral hemorrhagic fevers are considered possible bioterrorism agents because they are highly infectious, can be spread through the air as an aerosol or spray and would cause serious illness and fear in the population. In the event of a bioterrorist attack with VHFs, the Public Health Department ...
... Many viral hemorrhagic fevers are considered possible bioterrorism agents because they are highly infectious, can be spread through the air as an aerosol or spray and would cause serious illness and fear in the population. In the event of a bioterrorist attack with VHFs, the Public Health Department ...
Plant virus detection using aptamers
... leaves and general decline of infected plant. The virus is spread worldwide and often infects commercial cultures, which can remain latent without showing symptoms. Based on the examinations of various ASPV isolates there are more sequence variants. The main protein component of the virus is the coa ...
... leaves and general decline of infected plant. The virus is spread worldwide and often infects commercial cultures, which can remain latent without showing symptoms. Based on the examinations of various ASPV isolates there are more sequence variants. The main protein component of the virus is the coa ...
المسببات الفيروسية لإلتهاب الجهاز التنفسي لدى الأطفال في نجران
... include inconsistent clinical manifestations of viral RTI's and variable sensitivity and specificity of available diagnostic assays. The traditional diagnostic methods for the respiratory viruses including cell culture and direct immunofluorescence assays are time consuming and technically demanding ...
... include inconsistent clinical manifestations of viral RTI's and variable sensitivity and specificity of available diagnostic assays. The traditional diagnostic methods for the respiratory viruses including cell culture and direct immunofluorescence assays are time consuming and technically demanding ...
Development of Field-Based Real-Time Reverse Transcription
... A second re-emerging alphavirus, ONNV, was first isolated in 1959 and has been associated with relatively few outbreaks. However, several large-scale epidemics have occurred, including one during 1959–1962 in northern Uganda that involved more than two million cases.10,11 In 1996, ONNV reappeared af ...
... A second re-emerging alphavirus, ONNV, was first isolated in 1959 and has been associated with relatively few outbreaks. However, several large-scale epidemics have occurred, including one during 1959–1962 in northern Uganda that involved more than two million cases.10,11 In 1996, ONNV reappeared af ...
Role of viral load in the pathogenesis of chicken anemia virus
... pancreas [7?60–10?85 log10 copies (g tissue)21]. Organs with moderate loads were the liver [7?85–9?53 log10 copies (g tissue)21], spleen [7?60–9?25 log10 copies (g tissue)21], bursa [5?60–8?10 log10 copies (g tissue)21], kidney [6?60–7?85 log10 copies (g tissue)21] and lung [4?60–8?10 log10 copies ( ...
... pancreas [7?60–10?85 log10 copies (g tissue)21]. Organs with moderate loads were the liver [7?85–9?53 log10 copies (g tissue)21], spleen [7?60–9?25 log10 copies (g tissue)21], bursa [5?60–8?10 log10 copies (g tissue)21], kidney [6?60–7?85 log10 copies (g tissue)21] and lung [4?60–8?10 log10 copies ( ...
Evidence of existence of infectious hypodermal and hematopoietic
... infected broodstocks (Bell and Lightner, 1984; Lotz, 1997). Commonly recently L. vannamei becomes one of the major cultured species of aquaculture industry in China since the white spot disease outbroke in 1993. With importing broodstocks and postlarvae of animals from the countries which have the r ...
... infected broodstocks (Bell and Lightner, 1984; Lotz, 1997). Commonly recently L. vannamei becomes one of the major cultured species of aquaculture industry in China since the white spot disease outbroke in 1993. With importing broodstocks and postlarvae of animals from the countries which have the r ...
Viral shedding and antibody response in 37 patients with
... This is the author's manuscript of the article published in final edited form as: Corman, V. M., Albarrak, A. M., Omrani, A. S., Albarrak, M. M., Farah, M. E., Almasri, M., … Memish, Z. A. (2016). Viral Shedding and Antibody Response in 37 Patients With Middle East Respiratory Syndrome Coronavirus I ...
... This is the author's manuscript of the article published in final edited form as: Corman, V. M., Albarrak, A. M., Omrani, A. S., Albarrak, M. M., Farah, M. E., Almasri, M., … Memish, Z. A. (2016). Viral Shedding and Antibody Response in 37 Patients With Middle East Respiratory Syndrome Coronavirus I ...
Establishment of In-House Quantitative Real-Time RT
... ethics committees of Noguchi Memorial Institute for Medical Research, University of Ghana and Tokyo Medical and Dental University. Plasma separated from the whole blood was stored in individual cryo-vials at −80ºC until required for use. The mean age of the HIV-1 seronegative subjects was 27.5 years ...
... ethics committees of Noguchi Memorial Institute for Medical Research, University of Ghana and Tokyo Medical and Dental University. Plasma separated from the whole blood was stored in individual cryo-vials at −80ºC until required for use. The mean age of the HIV-1 seronegative subjects was 27.5 years ...
ELISA test reveals new information about leafroll disease
... that are rare in California have been described and ELISAs for them were not included. In all three experiments conducted in 1989,1990 and 1991, a positive ELISA result was obtained with tissue from selections that tested negative in the biological index on ’Cabernet Franc’. This suggests that eithe ...
... that are rare in California have been described and ELISAs for them were not included. In all three experiments conducted in 1989,1990 and 1991, a positive ELISA result was obtained with tissue from selections that tested negative in the biological index on ’Cabernet Franc’. This suggests that eithe ...
Piscirickettsia salm onis
... uses polyclonal antibodies that do not differentiate between isolates of P salmonis. Electron microscopy is time consuming and expensive, and organisms of similar morphology are difficult to differentiate. l? salmonis is sensitive to many of the antibiotics commonly used in cell culture media, makin ...
... uses polyclonal antibodies that do not differentiate between isolates of P salmonis. Electron microscopy is time consuming and expensive, and organisms of similar morphology are difficult to differentiate. l? salmonis is sensitive to many of the antibiotics commonly used in cell culture media, makin ...
Possible role of adeno-associated virus type 2 infection in
... Although miscarriage represents the most common complication in pregnancy and as such has been thoroughly investigated, the causes of miscarriages are still unexplained in the majority of cases. There are several indications connected to miscarriage, but only genetic and anatomical abnormalities are ...
... Although miscarriage represents the most common complication in pregnancy and as such has been thoroughly investigated, the causes of miscarriages are still unexplained in the majority of cases. There are several indications connected to miscarriage, but only genetic and anatomical abnormalities are ...
Molecular evolution of a viral non
... Fig. 4C), is characterized by positions causing low pathogenicity, with an average value ...
... Fig. 4C), is characterized by positions causing low pathogenicity, with an average value ...
Detection of Norwalk-like Virus in Shellfish Implicated in Illness
... mended sample processing procedure is to separate, process, and analyze environmental samples independently from clinical samples. Facilities and equipment designated for an environmental laboratory should not be used with clinical samples at any time; rather, clinical specimens, which often harbor ...
... mended sample processing procedure is to separate, process, and analyze environmental samples independently from clinical samples. Facilities and equipment designated for an environmental laboratory should not be used with clinical samples at any time; rather, clinical specimens, which often harbor ...
The development and control of Phytophthora infestans in Europe in
... increasingly more difficult to control (Turkensteen et al., 1997; Schepers, 2000). Resurgence of the late blight pathogen The increased problem with controlling potato late blight coincides with the displacement of the US-1 clonal lineage by a new, more variable P. infestans population in many parts ...
... increasingly more difficult to control (Turkensteen et al., 1997; Schepers, 2000). Resurgence of the late blight pathogen The increased problem with controlling potato late blight coincides with the displacement of the US-1 clonal lineage by a new, more variable P. infestans population in many parts ...
22_CePACT on the movement of Germplasm material
... also prompted a 5 year AusAID Taro Genetic Resources: Conservation and Utilisation (TaroGen) that began in 1998 and coordinated by SPC. The aim was to re-collect all taro genetic resources of the Pacific, have them characterized, DNA fingerprinted for selection of a core collection and have it viru ...
... also prompted a 5 year AusAID Taro Genetic Resources: Conservation and Utilisation (TaroGen) that began in 1998 and coordinated by SPC. The aim was to re-collect all taro genetic resources of the Pacific, have them characterized, DNA fingerprinted for selection of a core collection and have it viru ...
comparative study between elisa - the journal of the egyptian public
... specific test for detecting anti-BVD antibodies. Regarding the sensitivity and specificity of CBIA technique for detecting anti-BVD antibodies in the sera is in agreement with results of Zaghawa, (1993)(16) & (1997)(17), when they compared CBIA with serum neutralization test in detecting anti-canine ...
... specific test for detecting anti-BVD antibodies. Regarding the sensitivity and specificity of CBIA technique for detecting anti-BVD antibodies in the sera is in agreement with results of Zaghawa, (1993)(16) & (1997)(17), when they compared CBIA with serum neutralization test in detecting anti-canine ...
Applications of Real -Time PCR
... procedure. Using PCR, Bogen and Slots sought to determine the prevalence of P. endodontalis, P. gingivalis, P. intermédia and P. nigrescens in 20 with refractory periapical lesions. The authors concluded that bacteria that produce black pigment do not appear to constitute the majority of microorgani ...
... procedure. Using PCR, Bogen and Slots sought to determine the prevalence of P. endodontalis, P. gingivalis, P. intermédia and P. nigrescens in 20 with refractory periapical lesions. The authors concluded that bacteria that produce black pigment do not appear to constitute the majority of microorgani ...
Development of Multiplex-PCR Assay for the Rapid
... 14. Ilami O., et al. “A Detection of Neisseria gonorrhoeae and Chlamydia Trachomatis in patients with symptomatic urethritis using multiplex PCR, gram stain and urine culture”. Journal of Mazandaran University of Medical Sciences 23 (2013): 11-18. 15. Ahmadi MH., et al. “Prevalence of genital Chla ...
... 14. Ilami O., et al. “A Detection of Neisseria gonorrhoeae and Chlamydia Trachomatis in patients with symptomatic urethritis using multiplex PCR, gram stain and urine culture”. Journal of Mazandaran University of Medical Sciences 23 (2013): 11-18. 15. Ahmadi MH., et al. “Prevalence of genital Chla ...
Diagnostic Microbiology Using Real
... The hydrolysis or TaqMan probe chemistry depends on the 5 –3 exonuclease activity of the engineered Thermus aquaticus DNA-polymerase (Fig.18.2a). A DNA probe, labeled with a reporter dye and a quencher dye at opposite ends of the sequence, is designed to hybridize internal to the amplicon (Hiyoshi ...
... The hydrolysis or TaqMan probe chemistry depends on the 5 –3 exonuclease activity of the engineered Thermus aquaticus DNA-polymerase (Fig.18.2a). A DNA probe, labeled with a reporter dye and a quencher dye at opposite ends of the sequence, is designed to hybridize internal to the amplicon (Hiyoshi ...
Quantitative and Qualitative Changes of Phytochemical N. tabaccum
... W hite burley are rather restricted. This study analyzed Tobacco mosaic tobamovirus affecting plant growth and phytochemical compositions. Quantitative data on branch, leaves formation, stem growth and fresh and dry weights were systematically collected at three months. It was found that the branchi ...
... W hite burley are rather restricted. This study analyzed Tobacco mosaic tobamovirus affecting plant growth and phytochemical compositions. Quantitative data on branch, leaves formation, stem growth and fresh and dry weights were systematically collected at three months. It was found that the branchi ...
Potato virus Y
Potato virus Y (PVY) is a plant pathogenic virus of the family Potyviridae, and one of the most important plant viruses affecting potato production.PVY infection of potato plants results in a variety of symptoms depending on the viral strain. The mildest of these symptoms is production loss, but the most detrimental is 'potato tuber necrotic ringspot disease' (PTNRD). Necrotic ringspots render potatoes unmarketable and can therefore result in a significant loss of income. PVY is transmissible by aphid vectors but may also remain dormant in seed potatoes. This means that using the same line of potato for production of seed potatoes for several consecutive generations will lead to a progressive increase in viral load and subsequent loss of crop.An increase in potato plant infection with viruses over the past few years has led to considerable losses to the South African potato industry. The increased rate of infection may be attributed to several factors. These include a marked decrease in the effectiveness and administration of chemicals used in vector control, the use of infected seed potatoes in cultivation, incorrect irrigation and farming methods as well as a lack of a sensitive, rapid and reliable method of detection. An increase in the average temperature of winters as a consequence of global warming has also led to an increase in aphid numbers, which in turn has led to an increase in viral distribution.