HBTNCC 2011 a paper v3 August 2013
... RSV using the primers mixture for subgroup A and B or for PIV which used probes with the same quencher and ...
... RSV using the primers mixture for subgroup A and B or for PIV which used probes with the same quencher and ...
Arabis mosaic virus and Grapevine fanleaf virus (ArMV+
... communication). The coating reagent consists of poly- and monoclonal antibodies, the AP-conjugated antibodies are monoclonal. The ELISA technique is an efficient method for the detection of these viruses in grapevine. However, the virus concentration varies considerably according to the tissue source ...
... communication). The coating reagent consists of poly- and monoclonal antibodies, the AP-conjugated antibodies are monoclonal. The ELISA technique is an efficient method for the detection of these viruses in grapevine. However, the virus concentration varies considerably according to the tissue source ...
Molecular techniques for clinical diagnostic virology
... genomic DNA, but it is possible to amplify only a very few target viral genomes present to become the dominant, easily detected sequence. A variety of target amplification techniques has been developed but those most widely used and commercially developed are the polymerase chain reaction (PCR), lig ...
... genomic DNA, but it is possible to amplify only a very few target viral genomes present to become the dominant, easily detected sequence. A variety of target amplification techniques has been developed but those most widely used and commercially developed are the polymerase chain reaction (PCR), lig ...
LBC-101 Unit -1 Lect
... viral RNA directly serve as mRNA in the production of messenger in complex. After the coat protein and RNA genome have been synthesized they automatically assemble into complete TMV virion. The protomere come together to form disks composed of two layers of protomere arranged in a helical spiral. Af ...
... viral RNA directly serve as mRNA in the production of messenger in complex. After the coat protein and RNA genome have been synthesized they automatically assemble into complete TMV virion. The protomere come together to form disks composed of two layers of protomere arranged in a helical spiral. Af ...
hepatitis b virus
... Cont… About 5-10% of HBV infections result in chronic carrier state. The latter may be defined as persistence of HBsAg in the circulation for more than six months. Carriers are of two types: Super carriers: They have HBeAg, high titres of HBsAg and DNA polymerase in their blood. HBV may also be d ...
... Cont… About 5-10% of HBV infections result in chronic carrier state. The latter may be defined as persistence of HBsAg in the circulation for more than six months. Carriers are of two types: Super carriers: They have HBeAg, high titres of HBsAg and DNA polymerase in their blood. HBV may also be d ...
Molecular Diagnosis of Explosive Outbreak of Infectious
... the PCR directed to the TK gene of ILTV,the result were agreed with the characteristic symptoms of laryngotracheitis observed in the surveyed birds, as tracheitis, watery eyes and dyspnea. The PCR described in the present study is a usefulrapid tool to confirm the diagnosis of birds suspected of inf ...
... the PCR directed to the TK gene of ILTV,the result were agreed with the characteristic symptoms of laryngotracheitis observed in the surveyed birds, as tracheitis, watery eyes and dyspnea. The PCR described in the present study is a usefulrapid tool to confirm the diagnosis of birds suspected of inf ...
Isolation, identification and characterization of a tospovirus causing
... Phalaenopsis orchids plants bearing virus-like symptoms of chlorotic spots with centric necrosis or chlorotic ringspot on leaves have been observed in Taiwan for several years. Although the causal agent of this special disease was unclear, a so-called “Taiwan virus” was postulated to cause this dise ...
... Phalaenopsis orchids plants bearing virus-like symptoms of chlorotic spots with centric necrosis or chlorotic ringspot on leaves have been observed in Taiwan for several years. Although the causal agent of this special disease was unclear, a so-called “Taiwan virus” was postulated to cause this dise ...
Virus Disease Resistance in Peppers, A. A. Cook, Florida
... Bell pepper production in Florida has been affected considerably by virus diseases in re cent years (1, 15, 17). Seven different viruses are known to infect peppers under field con ditions, but only five have been isolated from plants grown in central Florida (1). These are the tobacco etch, potato ...
... Bell pepper production in Florida has been affected considerably by virus diseases in re cent years (1, 15, 17). Seven different viruses are known to infect peppers under field con ditions, but only five have been isolated from plants grown in central Florida (1). These are the tobacco etch, potato ...
Phylogenetic analysis of Lithuanian tomato black ring virus isolates
... Tomato black ring virus (TBRV) was first detected on tomato (Lycopersicon esculentum Mill.) plants in Lithuania in 2012. During 2013, additional eleven isolates of this virus were found on ornamental and food plants in different regions of Lithuania. The virus was identified by double antibody sandw ...
... Tomato black ring virus (TBRV) was first detected on tomato (Lycopersicon esculentum Mill.) plants in Lithuania in 2012. During 2013, additional eleven isolates of this virus were found on ornamental and food plants in different regions of Lithuania. The virus was identified by double antibody sandw ...
Progressive Right-Sided Hemiparesis in a Man
... Only select cell types express the specific polysialic acid receptor for the JC virus. In the central nervous system, this receptor is found on astrocytes and oligodendroglial lineages. JC viral entry occurs via clathrin-mediated endocytosis and can also be facilitated by serotonin receptors (subtype ...
... Only select cell types express the specific polysialic acid receptor for the JC virus. In the central nervous system, this receptor is found on astrocytes and oligodendroglial lineages. JC viral entry occurs via clathrin-mediated endocytosis and can also be facilitated by serotonin receptors (subtype ...
Background
... The determination of IgM can be made by different commercially available techniques (ELISA or IFA). However, it should be taken into account that the best sensitivity is from techniques that use the complete virus as antigen compared to those that use recombinant proteins (or peptides). It is recomm ...
... The determination of IgM can be made by different commercially available techniques (ELISA or IFA). However, it should be taken into account that the best sensitivity is from techniques that use the complete virus as antigen compared to those that use recombinant proteins (or peptides). It is recomm ...
Talan Proximity Ukraine Credentials
... guerilla marketing department when they were exchanged at credentials meetings, they created this low cost, highly effective business card stamp. ...
... guerilla marketing department when they were exchanged at credentials meetings, they created this low cost, highly effective business card stamp. ...
Complete laboratory diagnosis of Zika virus infections
... two main pillars: direct detection of the viral RNA genome and serological detection of anti-ZIKV antibodies. Direct detection of the virus by reverse transcriptase real-time polymerase chain reaction (RT real-time PCR) is the most important method for diagnosing early acute infections. A new RT rea ...
... two main pillars: direct detection of the viral RNA genome and serological detection of anti-ZIKV antibodies. Direct detection of the virus by reverse transcriptase real-time polymerase chain reaction (RT real-time PCR) is the most important method for diagnosing early acute infections. A new RT rea ...
Insects as a Vector of Plant Diseases
... degenerated growth. Tungro is caused by two viruses, an RNA virus, the rice tungro spherical virus (RTSV), and a DNA virus, the rice tungro bacilliform virus (RTBV). RTBV depends on the helper produced by RTSV for its transmission. It is the RTBV which is mainly responsible for the severe tungro sym ...
... degenerated growth. Tungro is caused by two viruses, an RNA virus, the rice tungro spherical virus (RTSV), and a DNA virus, the rice tungro bacilliform virus (RTBV). RTBV depends on the helper produced by RTSV for its transmission. It is the RTBV which is mainly responsible for the severe tungro sym ...
Late Blight in Tomatoes and Potatoes – Still a Concern for Gardeners
... You need to act quickly to protect your gardengrown tomato and potato plants and to make sure that your plants don’t become a source of spores that could infect commercial farms, as late blight spores are easily dispersed by wind. Cornell associate professor of plant pathology and plantmicrobe ...
... You need to act quickly to protect your gardengrown tomato and potato plants and to make sure that your plants don’t become a source of spores that could infect commercial farms, as late blight spores are easily dispersed by wind. Cornell associate professor of plant pathology and plantmicrobe ...
Rapid evolutionary dynamics of zucchini yellow mosaic virus
... ZYMV has a genome of 9593 nt arranged as a single open reading frame encoding a polyprotein precursor that is processed into 10 putative proteins (Gal-On, 2007). Of these, the coat protein (CP) is involved in the encapsidation The GenBank/EMBL/DDBJ accession numbers for the sequences reported in thi ...
... ZYMV has a genome of 9593 nt arranged as a single open reading frame encoding a polyprotein precursor that is processed into 10 putative proteins (Gal-On, 2007). Of these, the coat protein (CP) is involved in the encapsidation The GenBank/EMBL/DDBJ accession numbers for the sequences reported in thi ...
2004 Final Exam
... effect of various growth conditions on the amount of AMP present in cells (A). In addition you wish to examine what determines the rate of AMP biosynthesis in vitro using cell-free extracts and performing the reactions in a test tube (B). In order to make these measurements you incubate the whole ba ...
... effect of various growth conditions on the amount of AMP present in cells (A). In addition you wish to examine what determines the rate of AMP biosynthesis in vitro using cell-free extracts and performing the reactions in a test tube (B). In order to make these measurements you incubate the whole ba ...
Responses of Plants to Viruses - American Phytopathological Society
... symptoms or the occurrence of only mild symptoms, and little or no loss in vigor or yield. In these circumstances, tolerantis a term used to describe a HOST that a specific virus can infect and in which it can replicate and invade without causing severe symptoms or greatly diminishing the rate or am ...
... symptoms or the occurrence of only mild symptoms, and little or no loss in vigor or yield. In these circumstances, tolerantis a term used to describe a HOST that a specific virus can infect and in which it can replicate and invade without causing severe symptoms or greatly diminishing the rate or am ...
detection of st louis encephalitis and western equine
... toes infected on the day of death, that is, day 0 was 8O7o as determined by RT-PCR but only 407o as determined by plaque assay or in situ EIA. When NPS was used to kill SlE-infected Cx. tarsalis, the percent infected was one half of that seen after the use of triethylamine. Surveillance protocols th ...
... toes infected on the day of death, that is, day 0 was 8O7o as determined by RT-PCR but only 407o as determined by plaque assay or in situ EIA. When NPS was used to kill SlE-infected Cx. tarsalis, the percent infected was one half of that seen after the use of triethylamine. Surveillance protocols th ...
Notes about viruses
... A virus is not a cell and is not composed of cells. Viruses are smaller and simpler than cells. Approximate dimensions of viruses are 25nm to 250 nm, in contrast to bacteria, e.g., whose approximate dimensions are 0.5 μm to 5 μm. They are referred to as virus "particles;" a synonym is virion. The ty ...
... A virus is not a cell and is not composed of cells. Viruses are smaller and simpler than cells. Approximate dimensions of viruses are 25nm to 250 nm, in contrast to bacteria, e.g., whose approximate dimensions are 0.5 μm to 5 μm. They are referred to as virus "particles;" a synonym is virion. The ty ...
Occurrence and Distribution of Potato Pests and Diseases in Kenya
... factor of two were considered positive. Some plates were scored visually and samples considered positive if the color of substrate in the sample wells was clearly stronger than in the negative control wells. To assess aphid infestation, three leaves per plant (top, middle, and bottom) were collected ...
... factor of two were considered positive. Some plates were scored visually and samples considered positive if the color of substrate in the sample wells was clearly stronger than in the negative control wells. To assess aphid infestation, three leaves per plant (top, middle, and bottom) were collected ...
DETECTION AND IDENTIFICATION OF MICROORGANISMS
... Negative control: negative template Amplification control: omnipresent template unrelated to target ...
... Negative control: negative template Amplification control: omnipresent template unrelated to target ...
Plant Virus replication
... • Replicative form (RF)- it is fully base paired ds and may represent RNA molecules that have seized the replication • Replication intermediate (RI) is only partly ds and contains several ss tails. This structure is closely related to that actually replicating the viral RNA. ...
... • Replicative form (RF)- it is fully base paired ds and may represent RNA molecules that have seized the replication • Replication intermediate (RI) is only partly ds and contains several ss tails. This structure is closely related to that actually replicating the viral RNA. ...
Potato virus Y
Potato virus Y (PVY) is a plant pathogenic virus of the family Potyviridae, and one of the most important plant viruses affecting potato production.PVY infection of potato plants results in a variety of symptoms depending on the viral strain. The mildest of these symptoms is production loss, but the most detrimental is 'potato tuber necrotic ringspot disease' (PTNRD). Necrotic ringspots render potatoes unmarketable and can therefore result in a significant loss of income. PVY is transmissible by aphid vectors but may also remain dormant in seed potatoes. This means that using the same line of potato for production of seed potatoes for several consecutive generations will lead to a progressive increase in viral load and subsequent loss of crop.An increase in potato plant infection with viruses over the past few years has led to considerable losses to the South African potato industry. The increased rate of infection may be attributed to several factors. These include a marked decrease in the effectiveness and administration of chemicals used in vector control, the use of infected seed potatoes in cultivation, incorrect irrigation and farming methods as well as a lack of a sensitive, rapid and reliable method of detection. An increase in the average temperature of winters as a consequence of global warming has also led to an increase in aphid numbers, which in turn has led to an increase in viral distribution.