Lumpy Skin Disease Virus, Sheeppox Virus and Goatpox
... 4) Centrifuge the tube at 13000rpm for 10 minutes. The supernatant contains the DNA extracted and can be used for PCR template. 9.1.2 Tissue sample 1) Wash the tissue with sterile saline for several times. 2) Take 50mg sample in a tube, add 1ml sterile saline, and grind the tissue into homogenate. 3 ...
... 4) Centrifuge the tube at 13000rpm for 10 minutes. The supernatant contains the DNA extracted and can be used for PCR template. 9.1.2 Tissue sample 1) Wash the tissue with sterile saline for several times. 2) Take 50mg sample in a tube, add 1ml sterile saline, and grind the tissue into homogenate. 3 ...
Equine Herpes type 1 - European Commission
... African Horse Sickness Laboratory Diagnosis: Virus Isolation • Confirmation of an initial case of AHS in an area normally free of the disease requires isolation and identification of the virus. • AHSV can be isolated from heparinized blood, spleen, lymph node, or lung collected at necropsy using ce ...
... African Horse Sickness Laboratory Diagnosis: Virus Isolation • Confirmation of an initial case of AHS in an area normally free of the disease requires isolation and identification of the virus. • AHSV can be isolated from heparinized blood, spleen, lymph node, or lung collected at necropsy using ce ...
Belfast Health and Social Care Trust
... WHO influenza laboratory for diagnosis and research. The laboratory also maintains a unique epidemiological database of all diagnosed viral and atypical bacterial infections in Northern Ireland. The RVL has been at the forefront of developing and clinically validating new molecular diagnostic tests, ...
... WHO influenza laboratory for diagnosis and research. The laboratory also maintains a unique epidemiological database of all diagnosed viral and atypical bacterial infections in Northern Ireland. The RVL has been at the forefront of developing and clinically validating new molecular diagnostic tests, ...
Methods to Detect Microbes in the Environment ENVR 133 – Lecture
... – Macrorestriction analysis • Southern blotting and hybridization – Transfer DNA from gel to membrane (cellulose or ...
... – Macrorestriction analysis • Southern blotting and hybridization – Transfer DNA from gel to membrane (cellulose or ...
microbiology ch 43 [9-4
... Liver diseases similar to HBV but milder, especially in early stages of infection Following several decades of chronic HCV infection, incidence of cirrhosis about 20%; risk of HCC substantial Infection associated w/extrahepatic diseases (mixed cryoglobulinemia – immune complex disorder of kidn ...
... Liver diseases similar to HBV but milder, especially in early stages of infection Following several decades of chronic HCV infection, incidence of cirrhosis about 20%; risk of HCC substantial Infection associated w/extrahepatic diseases (mixed cryoglobulinemia – immune complex disorder of kidn ...
Myocarditis - Wellington ICU
... ECG: sinus tachycardia, non-specific ST elevation, TW changes ECHO essential myocardial biopsy: diagnosis based on Dallas criteria enterovirus PCR/serology parvovirus B19 PCR/serology HHV6 PCR/serology ...
... ECG: sinus tachycardia, non-specific ST elevation, TW changes ECHO essential myocardial biopsy: diagnosis based on Dallas criteria enterovirus PCR/serology parvovirus B19 PCR/serology HHV6 PCR/serology ...
SuperQuant™ HBV PCR - National Genetics Institute
... Baginski, I. Et al. (1990). Detection of hepatitis B virus. In: Innis, M.A. et al. eds. PCR Protocols: A Guide to Methods and ...
... Baginski, I. Et al. (1990). Detection of hepatitis B virus. In: Innis, M.A. et al. eds. PCR Protocols: A Guide to Methods and ...
Neuromythology and the Viral Etiologies of Multiple Sclerosis
... Epidemiology of MS: environmental exposure in childhood in genetically susceptible individuals Viral infection may be a co-factor interacting with the immune system immune stimulation from viral antigens and viral infection may be associated with persistent or reactivated viral infection ...
... Epidemiology of MS: environmental exposure in childhood in genetically susceptible individuals Viral infection may be a co-factor interacting with the immune system immune stimulation from viral antigens and viral infection may be associated with persistent or reactivated viral infection ...
1. Overview of Animal Viruses Life Cycle of Animal Viruses
... proceeds to produce + strands following uncoating ...
... proceeds to produce + strands following uncoating ...
Mag-Bind® Universal Pathogen 96 - Omega Bio-tek
... applications such as qPCR, next generation sequencing ...
... applications such as qPCR, next generation sequencing ...
Abstract
... between antigen preparations were small, and for practical reasons such as relative ease of production, economy, and high yield, the crude infected cell culture extract was chosen for subsequent use. Comparisons of ELISA and SNT antibody titres over a larger number of foal sera showed a high correla ...
... between antigen preparations were small, and for practical reasons such as relative ease of production, economy, and high yield, the crude infected cell culture extract was chosen for subsequent use. Comparisons of ELISA and SNT antibody titres over a larger number of foal sera showed a high correla ...
viral_rep_Hammer
... Viral Replication I. Steps in Viral Replication A. Attachment. This is the first step in viral replication. Surface proteins of the virus interact with specific receptors on the target cell surface. These may be specialized proteins with limited distribution or molecules that are more widely distrib ...
... Viral Replication I. Steps in Viral Replication A. Attachment. This is the first step in viral replication. Surface proteins of the virus interact with specific receptors on the target cell surface. These may be specialized proteins with limited distribution or molecules that are more widely distrib ...
HSV-1 and HSV-2: Chemotherapy
... Antivirals to treat HSV • Both acyclovir and pencyclovir work by interfering with viral replication, effectively slowing the replication rate of the virus, and providing a greater opportunity for the immune response to intervene. • All drugs in this class depend on the activity of the viral thymidin ...
... Antivirals to treat HSV • Both acyclovir and pencyclovir work by interfering with viral replication, effectively slowing the replication rate of the virus, and providing a greater opportunity for the immune response to intervene. • All drugs in this class depend on the activity of the viral thymidin ...
Virus and Viral Component Isolation
... reagent should be kept sealed and stored at 4C. Do not freeze. Viraffinity retains full activity when stored accordingly for approximately 1 year. ...
... reagent should be kept sealed and stored at 4C. Do not freeze. Viraffinity retains full activity when stored accordingly for approximately 1 year. ...
Diagnostic tests
... be further tested with an antigen ELISA (BVD) for confirmation. FAT (Fluorescent antibody test) The FAT is a rapid, specific diagnostic test for detecting viral antigen in tissues (thin sections) and cell smears. FAT is extremely dependent on the quality of clinical sample submitted and for best res ...
... be further tested with an antigen ELISA (BVD) for confirmation. FAT (Fluorescent antibody test) The FAT is a rapid, specific diagnostic test for detecting viral antigen in tissues (thin sections) and cell smears. FAT is extremely dependent on the quality of clinical sample submitted and for best res ...
Polymerase Chain Reaction Author(s): Gerald Schochetman, Chin
... of HIV-1 DNA in four men who had seroreverted out of 1000 asymptomatic homosexual men tested. Despite repeated attempts, the investigators did not succeed in culturing virus from cells of these men. The significance of these results remains to be determined. Rayfield et al. [27], as reported in this ...
... of HIV-1 DNA in four men who had seroreverted out of 1000 asymptomatic homosexual men tested. Despite repeated attempts, the investigators did not succeed in culturing virus from cells of these men. The significance of these results remains to be determined. Rayfield et al. [27], as reported in this ...
Antiviral Drugs
... and for Acyclovir-resistant PK: produce active metabolite, thus it has long t1/2, eliminated in the kidney, Probenicid prolonged its action. but with poor CNS penetration. Why acycoliver is better than Cidofovir for encephalitis? Side effects: Dose-dependent nephrotoxicity (53%) and uveitis and decr ...
... and for Acyclovir-resistant PK: produce active metabolite, thus it has long t1/2, eliminated in the kidney, Probenicid prolonged its action. but with poor CNS penetration. Why acycoliver is better than Cidofovir for encephalitis? Side effects: Dose-dependent nephrotoxicity (53%) and uveitis and decr ...
Paramyxoviruses
... 1- German measles: acute febrile illness with rash & lymphadenopathy affecting children & young adults 2- Congenital Rubella Syndrome: Serious abnormalities of the fetus as a consequence of maternal infection during early pregnancy ...
... 1- German measles: acute febrile illness with rash & lymphadenopathy affecting children & young adults 2- Congenital Rubella Syndrome: Serious abnormalities of the fetus as a consequence of maternal infection during early pregnancy ...
Document
... Ranavirus can be detected using PCR and q-PCR protocol that was developed and can help in rapid detection. ...
... Ranavirus can be detected using PCR and q-PCR protocol that was developed and can help in rapid detection. ...
Respiratory Viral Panel testing by Multiplex PCR
... Multiplex Polymerase Chain Reaction (PCR) detection offers a highly sensitive and specific method of respiratory virus detection and is quickly replacing culture and DFA as the gold standard. In addition to sensitivity and specificity, benefits of multiplex PCR testing include the ability to obtain ...
... Multiplex Polymerase Chain Reaction (PCR) detection offers a highly sensitive and specific method of respiratory virus detection and is quickly replacing culture and DFA as the gold standard. In addition to sensitivity and specificity, benefits of multiplex PCR testing include the ability to obtain ...
Hepatitis A and B
... 1:1000 of case may develop fulminant hepatitis. 10 become chronic infection: chronic antigenaemia: patient fail to form anti HBs and delayed anti Hbe. HBsAg persist in blood, patient is well, liver function is normal. Chronic active (aggressive) hepatitis: patient fail to produce anti HBs and anti H ...
... 1:1000 of case may develop fulminant hepatitis. 10 become chronic infection: chronic antigenaemia: patient fail to form anti HBs and delayed anti Hbe. HBsAg persist in blood, patient is well, liver function is normal. Chronic active (aggressive) hepatitis: patient fail to produce anti HBs and anti H ...
Viral Replication Viral Replication: Basic Concepts
... steps in replication cycle • Viruses depend on host cell machinery to complete replication cycle and must commandeer that machinery to successfully replicate ...
... steps in replication cycle • Viruses depend on host cell machinery to complete replication cycle and must commandeer that machinery to successfully replicate ...
2004 - Missouri State University
... The Polymerase Chain Reaction (PCR) method involves the conversion of RNA into cDNA (complementary DNA) and then the exponential replication of strands of the DNA. Once a large amount of the DNA has been produced, is it placed at the top of a column containing a gel. The DNA moves downward through ...
... The Polymerase Chain Reaction (PCR) method involves the conversion of RNA into cDNA (complementary DNA) and then the exponential replication of strands of the DNA. Once a large amount of the DNA has been produced, is it placed at the top of a column containing a gel. The DNA moves downward through ...
Detection of infectious hematopoietic necrosis virus in rainbow trout
... IHN viral disease in Taiwan. During these years, the rainbow trout culture industry has become popular in the high mountain area so that numerous eyed eggs have been imported from North America, Japan, and other countries every year, creating many chances for IHNV to be transmitted into Taiwan. Howe ...
... IHN viral disease in Taiwan. During these years, the rainbow trout culture industry has become popular in the high mountain area so that numerous eyed eggs have been imported from North America, Japan, and other countries every year, creating many chances for IHNV to be transmitted into Taiwan. Howe ...
Potato virus Y
Potato virus Y (PVY) is a plant pathogenic virus of the family Potyviridae, and one of the most important plant viruses affecting potato production.PVY infection of potato plants results in a variety of symptoms depending on the viral strain. The mildest of these symptoms is production loss, but the most detrimental is 'potato tuber necrotic ringspot disease' (PTNRD). Necrotic ringspots render potatoes unmarketable and can therefore result in a significant loss of income. PVY is transmissible by aphid vectors but may also remain dormant in seed potatoes. This means that using the same line of potato for production of seed potatoes for several consecutive generations will lead to a progressive increase in viral load and subsequent loss of crop.An increase in potato plant infection with viruses over the past few years has led to considerable losses to the South African potato industry. The increased rate of infection may be attributed to several factors. These include a marked decrease in the effectiveness and administration of chemicals used in vector control, the use of infected seed potatoes in cultivation, incorrect irrigation and farming methods as well as a lack of a sensitive, rapid and reliable method of detection. An increase in the average temperature of winters as a consequence of global warming has also led to an increase in aphid numbers, which in turn has led to an increase in viral distribution.