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... disc cells by stimulating the expression of matrix metalloproteinases, leading to disc degeneration. To determine if this is a cause of apoptosis (select disc cell death), rat annular cells were cultured with or without serum supplement. First-passage rat annular cells were cultured with 0% or 10% f ...
... disc cells by stimulating the expression of matrix metalloproteinases, leading to disc degeneration. To determine if this is a cause of apoptosis (select disc cell death), rat annular cells were cultured with or without serum supplement. First-passage rat annular cells were cultured with 0% or 10% f ...
Biosynthesis and degradation of proteins
... • IAPs are proteins that block apoptosis by binding to and inhibiting caspases. The apoptosis-stimulating protein Smac antagonizes the effect of IAPs on caspases. • TIMPs are inhibitors of metalloproteases that are secreted by cells. A domain of the inhibitor protein interacts with the catalytic Zn+ ...
... • IAPs are proteins that block apoptosis by binding to and inhibiting caspases. The apoptosis-stimulating protein Smac antagonizes the effect of IAPs on caspases. • TIMPs are inhibitors of metalloproteases that are secreted by cells. A domain of the inhibitor protein interacts with the catalytic Zn+ ...
powerpoint
... Mechanisms governing the secondary burst of reactive oxygen species (ROS) and basic pathways of cell death from hyperoxia. 1: Loss of plasma membrane integrity from lipid peroxidation by ROS. 2: ROS damage to the mitochondria membranes and deactivation of enzyme systems and cytochrome chain. 3: This ...
... Mechanisms governing the secondary burst of reactive oxygen species (ROS) and basic pathways of cell death from hyperoxia. 1: Loss of plasma membrane integrity from lipid peroxidation by ROS. 2: ROS damage to the mitochondria membranes and deactivation of enzyme systems and cytochrome chain. 3: This ...
Clusterin as a novel Bcl-2 Homology 3 (BH3)
... ethanol-induced nuclear overexpression of clusterin does not protect cells, but rather leads to cell death. Clusterin (Clu) is ubiquitously expressed and implicated in diverse, yet contrasting, cellular processes such as apoptosis and anti-apoptosis1,2. Whereas Clu is known to inhibit the proapoptot ...
... ethanol-induced nuclear overexpression of clusterin does not protect cells, but rather leads to cell death. Clusterin (Clu) is ubiquitously expressed and implicated in diverse, yet contrasting, cellular processes such as apoptosis and anti-apoptosis1,2. Whereas Clu is known to inhibit the proapoptot ...
Apoptosis
... Fig. Activation of caspase-3 and proteolytic cleavage of p21WAF1/Cip1 and PARP After treatment with the indicated concentrations of apicidin for 48 h, the cleavage of procaspase-3, PARP, and p21WAF1/Cip1 were examined by Western blot analysis. Figure 18-5b Molecular Biology of the Cell (© Garland S ...
... Fig. Activation of caspase-3 and proteolytic cleavage of p21WAF1/Cip1 and PARP After treatment with the indicated concentrations of apicidin for 48 h, the cleavage of procaspase-3, PARP, and p21WAF1/Cip1 were examined by Western blot analysis. Figure 18-5b Molecular Biology of the Cell (© Garland S ...
Anti-DR3, Extracellular Domain (D3688) - Data Sheet - Sigma
... acids 59-77 in the extracellular domain (ED) of human DR3 precursor.1,2 Anti-DR3, Extracellular Domain specifically recognizes the extracellular domain of death receptor 3 in human cells by immunoblotting. Apoptosis or programmed cell death is induced in cells by a group of death domain-containing r ...
... acids 59-77 in the extracellular domain (ED) of human DR3 precursor.1,2 Anti-DR3, Extracellular Domain specifically recognizes the extracellular domain of death receptor 3 in human cells by immunoblotting. Apoptosis or programmed cell death is induced in cells by a group of death domain-containing r ...
Radiobiology Lec:3 Stage:2 3.Cell death after irradiation
... Apoptosis is an important cellular defence against cancer development and loss of apoptotic sensitivityis recognized as an essential hallmark of cancer. Apoptosis can be initiated either as a result of conditions occurring within the cell itself (such as those after DNA damage) or from signals gener ...
... Apoptosis is an important cellular defence against cancer development and loss of apoptotic sensitivityis recognized as an essential hallmark of cancer. Apoptosis can be initiated either as a result of conditions occurring within the cell itself (such as those after DNA damage) or from signals gener ...
Erdal, Hamdiye et al. (2005) Proc. Natl. Acad. Sci. USA 102, 192
... require an acidic environment for efficient function. The lysosome maintains a pH close to 5.0. ...
... require an acidic environment for efficient function. The lysosome maintains a pH close to 5.0. ...
Daniel Kaganovich Molecular Mechanism of
... models, we develop animal models of neural function and neurodegenerative disease. Our goal is to understand some of the ways in which neurons manage protein folding differently form other cells, and how this contributes to the pathology of diseases such as ALS, Alzheimer’s, Parkinson’s, and Hunding ...
... models, we develop animal models of neural function and neurodegenerative disease. Our goal is to understand some of the ways in which neurons manage protein folding differently form other cells, and how this contributes to the pathology of diseases such as ALS, Alzheimer’s, Parkinson’s, and Hunding ...
Multiple Effects of Hymenocrater longiflorus on human colon cancer
... Immunoflourescence staining for γ-H2AX on RKO cell line at 4 hrs for 80 and 100µg ml-1 H. longiflorus plant extract in compares with CPT, UV and NT cells. Also during treatment with plant extract at different times and concentrations observe increasing in proteins like P21, P53, P53-P, γ-H2AX and cl ...
... Immunoflourescence staining for γ-H2AX on RKO cell line at 4 hrs for 80 and 100µg ml-1 H. longiflorus plant extract in compares with CPT, UV and NT cells. Also during treatment with plant extract at different times and concentrations observe increasing in proteins like P21, P53, P53-P, γ-H2AX and cl ...
cells by Activating the Intrinsic and Extrinsic Death Pathway
... show that AW-464, an inhibitor of intracellular Trx activity, reduces inflammatory activation of A549 cells in response to certain inflammatory mediators. These effects do not reflect general inhibition of protein synthesis (as AW-464 had no effect on ICAM-I induced by IFNy) or a reduction in cell v ...
... show that AW-464, an inhibitor of intracellular Trx activity, reduces inflammatory activation of A549 cells in response to certain inflammatory mediators. These effects do not reflect general inhibition of protein synthesis (as AW-464 had no effect on ICAM-I induced by IFNy) or a reduction in cell v ...
P53 genes HW - St Paul`s School Intranet
... Produces a protein which acts as a “transcription factor”, switching on a number of other genes. One of these is p21. Some people inherit a defective p53 allele from one of their parents, so each cell has only one working copy. Accidental damage to this copy (during replication or due to ionising ra ...
... Produces a protein which acts as a “transcription factor”, switching on a number of other genes. One of these is p21. Some people inherit a defective p53 allele from one of their parents, so each cell has only one working copy. Accidental damage to this copy (during replication or due to ionising ra ...
Contents - Molecular Cancer Therapeutics
... Several allosteric MEK inhibitors are in clinical development and have been designed to treat patients with tumors harboring RAS/RAF pathway alterations. Acquired resistance to this class of inhibitors is a pressing clinical problem. To identify strategies to overcome this resistance, Hatzivassiliou ...
... Several allosteric MEK inhibitors are in clinical development and have been designed to treat patients with tumors harboring RAS/RAF pathway alterations. Acquired resistance to this class of inhibitors is a pressing clinical problem. To identify strategies to overcome this resistance, Hatzivassiliou ...
Table S2. Integration of Trypanosoma cruzi kDNA minicircle
... Serine/threonine protein kinase (PI-3K) involved in mRNA surveillance and genotoxic stress response. Plays a role in nonsense-mediated decay of mRNAs by phosphorylating UPF1/RENT1. Acts as part of the SMG1C complex, a mRNA surveillance complex that recognizes and degrades mRNAs containing premature ...
... Serine/threonine protein kinase (PI-3K) involved in mRNA surveillance and genotoxic stress response. Plays a role in nonsense-mediated decay of mRNAs by phosphorylating UPF1/RENT1. Acts as part of the SMG1C complex, a mRNA surveillance complex that recognizes and degrades mRNAs containing premature ...
CRYSTAL STRUCTURE OF AN ENZYME INVOLVED IN THE
... Isoprenoids are a diverse family of compounds consisting of isoprene units (fivecarbons units) and are involved in many biological functions such as electron transport, hormone based signaling, apoptosis, also they provide structural components of cell membranes. In contrast to mammals, some pathoge ...
... Isoprenoids are a diverse family of compounds consisting of isoprene units (fivecarbons units) and are involved in many biological functions such as electron transport, hormone based signaling, apoptosis, also they provide structural components of cell membranes. In contrast to mammals, some pathoge ...
Gene Section NLRC4 (NLR Family, CARD domain containing 4)
... It is involved in restriction of Legionella replication through the regulation of phagosome maturation. NLRC4 knock out mice are resistant to Salmonella typhimurium induced endotoxic shock. NLRC4 is one of the mediators of the p53-induced apoptosis. It is also known to have caspase-1 independent fun ...
... It is involved in restriction of Legionella replication through the regulation of phagosome maturation. NLRC4 knock out mice are resistant to Salmonella typhimurium induced endotoxic shock. NLRC4 is one of the mediators of the p53-induced apoptosis. It is also known to have caspase-1 independent fun ...
Apoptosis
... Biochemical Events in Apoptosis • Caspases (cysteine proteases) cleave the cytoskeleton and activate DNAses and other enzymes • DNA breaks into 50- to 300-kilobase pieces; further broken into multiples of 200 base pairs by endonucleases (Ca++ and Mg++)- demonstrated as a “ladder pattern” on agarose ...
... Biochemical Events in Apoptosis • Caspases (cysteine proteases) cleave the cytoskeleton and activate DNAses and other enzymes • DNA breaks into 50- to 300-kilobase pieces; further broken into multiples of 200 base pairs by endonucleases (Ca++ and Mg++)- demonstrated as a “ladder pattern” on agarose ...
emerging molecular mechanisms of pulmonary arterial hypertension
... Increasingly, evidence is pointing to endothelial cell (EC) apoptosis as a critical trigger in the pathogenesis of PAH. Loss-of-function mutations in the bone morphogenetic protein receptor 2 (Bmpr2) genes have been linked with familial PAH, and this receptor has been recently shown to mediate EC su ...
... Increasingly, evidence is pointing to endothelial cell (EC) apoptosis as a critical trigger in the pathogenesis of PAH. Loss-of-function mutations in the bone morphogenetic protein receptor 2 (Bmpr2) genes have been linked with familial PAH, and this receptor has been recently shown to mediate EC su ...
From Biomarkers to Companion Diagnostics: Mitochondrial
... metabolic re-programming of cancer cells includes marked over-expression of the isoforms of the mitochondrialbound glycolytic protein hexokinase (HK), required for energy production. HK also acts as an anti-apoptotic protein via a direct association with VDAC1 localized at the outer mitochondrial me ...
... metabolic re-programming of cancer cells includes marked over-expression of the isoforms of the mitochondrialbound glycolytic protein hexokinase (HK), required for energy production. HK also acts as an anti-apoptotic protein via a direct association with VDAC1 localized at the outer mitochondrial me ...
Cullen Lecture 6: Signal Transduction in Fungi Filamentous Growth
... Msb2 w/o the Mucin Domain is Hyperactive ...
... Msb2 w/o the Mucin Domain is Hyperactive ...
" Exploring the Unique Dual Function and the Evolutionary
... essential in pathogenic parasites since it is directly linked to vital parasite-specific processes, e.g. host cell invasion, nutrition, and cell differentiation into resistant stages, as in the case of Giardia. Recently, we have identified a protein containing an ENTH domain that defines monomeric a ...
... essential in pathogenic parasites since it is directly linked to vital parasite-specific processes, e.g. host cell invasion, nutrition, and cell differentiation into resistant stages, as in the case of Giardia. Recently, we have identified a protein containing an ENTH domain that defines monomeric a ...
Galectin
... phosphorylation of Ser6 (Yoshii et al, 2002) Galectin-3 proposed to play a role in b2-integrinindependent neutrophil extravasation (Sato et al, 2002) During involution of the mammary gland galectin-3 expression is up-regulated and occurs in non-apoptotic cells (Mengwasser et al, 2002) ...
... phosphorylation of Ser6 (Yoshii et al, 2002) Galectin-3 proposed to play a role in b2-integrinindependent neutrophil extravasation (Sato et al, 2002) During involution of the mammary gland galectin-3 expression is up-regulated and occurs in non-apoptotic cells (Mengwasser et al, 2002) ...
lecture 8
... programmed cell death in a negative way by causing T helper cells that typically fight off viruses and other infections in the human body to begin self-destructing. This condition, referred to as acquired immunodeficiency syndrome (AIDS), effectively destroys the body’s immune system, which often le ...
... programmed cell death in a negative way by causing T helper cells that typically fight off viruses and other infections in the human body to begin self-destructing. This condition, referred to as acquired immunodeficiency syndrome (AIDS), effectively destroys the body’s immune system, which often le ...
Apoptosome
The apoptosome is a large quaternary protein structure formed in the process of apoptosis. Its formation is triggered by the release of cytochrome c from the mitochondria in response to an internal (intrinsic) or external (extrinsic) cell death stimulus. Stimuli can vary from DNA damage and viral infection to developmental cues such as those leading to the degradation of a tadpole's tail.In mammalian cells, once cytochrome c is released, it binds to the cytosolic protein Apaf-1 to facilitate the formation of apoptosome. An early biochemical study suggests a two-to-one ratio of cytochrome c to apaf-1 for apoptosome formation. However, recent structural studies suggest the cytochrome c to apaf-1 ratio is one-to-one. It has also been shown that the nucleotide dATP as third component binds to apaf-1, however its exact role is still debated. The mammalian apoptosome had never been crystallized, but a human APAF-1/cytochrome-c apoptosome has been imaged at lower (2 nm) resolution by cryogenic transmission electron microscopy 10 years ago, revealing a wheel-like particle with 7-fold symmetry. Recently, a medium resolution (9.5 Ångström) structure of human apoptosome was also solved by cryo-electron microscopy, which allows unambiguous inference for positions of all the APAF-1 domains (CARD, NBARC and WD40) and cytochrome c. There is also now a crystal structure of the monomeric, inactive Apaf-1 subunit (PDB 3SFZ). Once formed, the apoptosome can then recruit and activate the inactive pro-caspase-9. Once activated, this initiator caspase can then activate effector caspases and trigger a cascade of events leading to apoptosis.