Paper Plasmid activity - Liberty Union High School District
... 6. The HindIII & EcoR1 restriction enzyme cutting sites (sequences of bases) are marked in bold on the Jellyfish Glo gene DNA. 7. The two restriction enzymes and their respective restriction sites are listed below. These restriction enzymes act as “molecular scissors” to cut the DNA at these sequenc ...
... 6. The HindIII & EcoR1 restriction enzyme cutting sites (sequences of bases) are marked in bold on the Jellyfish Glo gene DNA. 7. The two restriction enzymes and their respective restriction sites are listed below. These restriction enzymes act as “molecular scissors” to cut the DNA at these sequenc ...
Fluorescent Protein Transformation Student Background
... its normal existence, bacteria naturally contain one or more tiny circular pieces of DNA called plasmids. Plasmid DNA contains genes for traits that may be beneficial to bacterial survival under certain environmental conditions. In nature, bacteria can transfer plasmids back and forth, allowing them ...
... its normal existence, bacteria naturally contain one or more tiny circular pieces of DNA called plasmids. Plasmid DNA contains genes for traits that may be beneficial to bacterial survival under certain environmental conditions. In nature, bacteria can transfer plasmids back and forth, allowing them ...
Chapter 13: Genetic Engineering
... opposite sites without overhangs. SmaI is an example of an enzyme that generates blunt ends ...
... opposite sites without overhangs. SmaI is an example of an enzyme that generates blunt ends ...
CHEMISTRY
... 18.6. What is bacterial transformation and how does it happen? 18.7. Using a diagram, describe the process of transduction by viruses and mention some implications and uses of this process. 18.8. Explain what plasmids are and describe how they can be transferred from one bacterial cell to another. 1 ...
... 18.6. What is bacterial transformation and how does it happen? 18.7. Using a diagram, describe the process of transduction by viruses and mention some implications and uses of this process. 18.8. Explain what plasmids are and describe how they can be transferred from one bacterial cell to another. 1 ...
Microbiology Lab Manual
... variability and offspring with completely new combinations of genes. Genetic changes in bacteria changes are often associated with factors that increase pathogenicity by equipping the bacteria with additional abilities to produce toxins, evade the immune system, or resist antibiotics. While eukaryot ...
... variability and offspring with completely new combinations of genes. Genetic changes in bacteria changes are often associated with factors that increase pathogenicity by equipping the bacteria with additional abilities to produce toxins, evade the immune system, or resist antibiotics. While eukaryot ...
ANSWER KEY
... 16.) Which procedure(s) will most likely yield transformants? Why or why not? (8pts) Only procedure ii would have a chance to yield transformants because naturally competent cells bind to double stranded DNA and take it up in linear form. 17.) Which of the above substrates would yield transformants ...
... 16.) Which procedure(s) will most likely yield transformants? Why or why not? (8pts) Only procedure ii would have a chance to yield transformants because naturally competent cells bind to double stranded DNA and take it up in linear form. 17.) Which of the above substrates would yield transformants ...
PowerPoint Presentation - No Slide Title
... radioactive probe can be used to identify colonies that carry a plasmid that has an insert that is complementary to the probe. The single-stranded probe base pairs to any plasmid DNA that has complementary sequence. The fact that it is radioactive makes it easy to see where it went. ...
... radioactive probe can be used to identify colonies that carry a plasmid that has an insert that is complementary to the probe. The single-stranded probe base pairs to any plasmid DNA that has complementary sequence. The fact that it is radioactive makes it easy to see where it went. ...
Clike here - University of Evansville Faculty Web sites
... radioactive probe can be used to identify colonies that carry a plasmid that has an insert that is complementary to the probe. The single-stranded probe base pairs to any plasmid DNA that has complementary sequence. The fact that it is radioactive makes it easy to see where it went. ...
... radioactive probe can be used to identify colonies that carry a plasmid that has an insert that is complementary to the probe. The single-stranded probe base pairs to any plasmid DNA that has complementary sequence. The fact that it is radioactive makes it easy to see where it went. ...
LECTURE 16 – Using Genomic Variation for Identity DNA Level
... Ø Plasmids – vectors that carry genes in bacteria, they are self replicating circles of DNA Ø Antibiotic resistance – select for a recombinant DNA molecule Ø Restriction enzymes – cut DNA molecules at specific sequence Ø DNA ligase – join DNA fragments together Ø Transformation – uptake of exogenous ...
... Ø Plasmids – vectors that carry genes in bacteria, they are self replicating circles of DNA Ø Antibiotic resistance – select for a recombinant DNA molecule Ø Restriction enzymes – cut DNA molecules at specific sequence Ø DNA ligase – join DNA fragments together Ø Transformation – uptake of exogenous ...
Genetic Manipulation of Bacteria
... Schwab H. (1993) Principles of Genetic Engineering for Escherichia coli. In Biotechnology [eds. H.J.Rehm and G.Reed], VCH Weinhein, Volume 2: A.Puhler Genetic Fundamentals and Genetic Engineering. Another source covering the general principles of gentic manipulation in the best known bacterial syste ...
... Schwab H. (1993) Principles of Genetic Engineering for Escherichia coli. In Biotechnology [eds. H.J.Rehm and G.Reed], VCH Weinhein, Volume 2: A.Puhler Genetic Fundamentals and Genetic Engineering. Another source covering the general principles of gentic manipulation in the best known bacterial syste ...
MB 206 Microbial Biotechnology2
... - Contain a selectable marker to distinguish host cells containing the vector from amongst those that do not (eg. antibiotic resistancy or survival under certain growth conditions. ...
... - Contain a selectable marker to distinguish host cells containing the vector from amongst those that do not (eg. antibiotic resistancy or survival under certain growth conditions. ...
File
... other vectors, including the other two classes of vector for E. coli: plasmids and phage λ Filamentous bacteriophages have a number of unique properties that make them suitable as vectors ...
... other vectors, including the other two classes of vector for E. coli: plasmids and phage λ Filamentous bacteriophages have a number of unique properties that make them suitable as vectors ...
Chapter 3 part I
... Clones with foreign DNA in the MCS disrupt the ability of the cells to make β-galactosidase Plate on media with a β-galactosidase indicator (X-gal) and clones with intact β-galactosidase enzyme will produce blue ...
... Clones with foreign DNA in the MCS disrupt the ability of the cells to make β-galactosidase Plate on media with a β-galactosidase indicator (X-gal) and clones with intact β-galactosidase enzyme will produce blue ...
Genetics and Genetic Engineering
... makeup of plants and animals allow them to respond to changes in their environment ...
... makeup of plants and animals allow them to respond to changes in their environment ...
How can jellyfish shed light on the subject? One of the
... them to glow a brilliant green color under ultraviolet light. In this activity, students will learn about the process of moving genes from one organism to another with the aid of a plasmid. In addition to one large chromosome, bacteria naturally contain one or more small circular pieces of DNA calle ...
... them to glow a brilliant green color under ultraviolet light. In this activity, students will learn about the process of moving genes from one organism to another with the aid of a plasmid. In addition to one large chromosome, bacteria naturally contain one or more small circular pieces of DNA calle ...
Gene Cloning
... – This technique depends on base pairing between our gene and a short piece of DNA or RNA with a complementary sequence to the gene called a Probe, – The sequence of our RNA or DNA probe depends on knowledge of at least part of the sequence of our gene. – A radioactive or fluorescent tag labels the ...
... – This technique depends on base pairing between our gene and a short piece of DNA or RNA with a complementary sequence to the gene called a Probe, – The sequence of our RNA or DNA probe depends on knowledge of at least part of the sequence of our gene. – A radioactive or fluorescent tag labels the ...
Hydrogen autotrophy of Nocardia opaca strains is
... slightly above the largest A HindIII fragment (Fig. 2). In lysates of N . opaca obtained by alkaline treatment, only cccDNA plasmids became visible (Sensfuss et al., 1986). Whereas the mobility of the linear plasmids was dependent on the pulse time, that of cccDNA was not affected when CHEFE was emp ...
... slightly above the largest A HindIII fragment (Fig. 2). In lysates of N . opaca obtained by alkaline treatment, only cccDNA plasmids became visible (Sensfuss et al., 1986). Whereas the mobility of the linear plasmids was dependent on the pulse time, that of cccDNA was not affected when CHEFE was emp ...
general steps of gene cloning
... Recombinant DNA: A DNA molecule produced by inserting DNA from one organism (or may be chemically synthesized) into another piece of DNA using gene manipulation techniques. Restriction endonucleases: Bacterial enzymes that cut (hydrolyze) DNA at specific recognition sequences into defined and reprod ...
... Recombinant DNA: A DNA molecule produced by inserting DNA from one organism (or may be chemically synthesized) into another piece of DNA using gene manipulation techniques. Restriction endonucleases: Bacterial enzymes that cut (hydrolyze) DNA at specific recognition sequences into defined and reprod ...
Lecture-Mic 623-Plasmids-Corynebacterium - Home
... 3-8 X 10(3) plaque-forming units/micrograms of phage DNA, and transformation frequencies were in the range of 0.2-150 colony-forming units/micrograms of plasmid DNA. Plasmid pNG2 replicated and was stably maintained in all transformants both in the presence or absence of erythromycin. Thus, it displ ...
... 3-8 X 10(3) plaque-forming units/micrograms of phage DNA, and transformation frequencies were in the range of 0.2-150 colony-forming units/micrograms of plasmid DNA. Plasmid pNG2 replicated and was stably maintained in all transformants both in the presence or absence of erythromycin. Thus, it displ ...
Organization of the eukaryotic genomes
... that of prokaryotic cells. The genomes of prokaryotes are contained in single chromosomes, which are usually circular DNA molecules. In contrast, the genomes of eukaryotes are composed of multiple chromosomes, each containing a linear molecular of DNA. ...
... that of prokaryotic cells. The genomes of prokaryotes are contained in single chromosomes, which are usually circular DNA molecules. In contrast, the genomes of eukaryotes are composed of multiple chromosomes, each containing a linear molecular of DNA. ...
Cloning Power Point
... multiple copies of that gene using bacterial plasmids, selfreplicating extra-chromosomal circular DNA molecules, that are distinctly different from the normal bacterial genome. Genes and other chromosomes are copied to make enough samples for further study. In order to clone a gene, a fragment of DN ...
... multiple copies of that gene using bacterial plasmids, selfreplicating extra-chromosomal circular DNA molecules, that are distinctly different from the normal bacterial genome. Genes and other chromosomes are copied to make enough samples for further study. In order to clone a gene, a fragment of DN ...
Expression and Purification of Recombinant Protein in bacteria and
... Yeast Artificial Chromosomes Phage lambda vectors Bacterial Artificial Chromosomes Human Artificial Chromosomes Transposons etc. ...
... Yeast Artificial Chromosomes Phage lambda vectors Bacterial Artificial Chromosomes Human Artificial Chromosomes Transposons etc. ...
Plasmid
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A plasmid is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small, circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.Plasmids are considered replicons, a unit of DNA capable of replicating autonomously within a suitable host. However, plasmids, like viruses, are not generally classified as life. Plasmids can be transmitted from one bacterium to another (even of another species) via three main mechanisms: transformation, transduction, and conjugation. This host-to-host transfer of genetic material is called horizontal gene transfer, and plasmids can be considered part of the mobilome. Unlike viruses (which encase their genetic material in a protective protein coat called a capsid), plasmids are ""naked"" DNA and do not encode genes necessary to encase the genetic material for transfer to a new host. However, some classes of plasmids encode the conjugative ""sex"" pilus necessary for their own transfer. The size of the plasmid varies from 1 to over 200 kbp, and the number of identical plasmids in a single cell can range anywhere from one to thousands under some circumstances.The relationship between microbes and plasmid DNA is neither parasitic nor mutualistic, because each implies the presence of an independent species living in a detrimental or commensal state with the host organism. Rather, plasmids provide a mechanism for horizontal gene transfer within a population of microbes and typically provide a selective advantage under a given environmental state. Plasmids may carry genes that provide resistance to naturally occurring antibiotics in a competitive environmental niche, or the proteins produced may act as toxins under similar circumstances, or allow the organism to utilize particular organic compounds that would be advantageous when nutrients are scarce.