4.4 Genetic Engineering and Biotechnology
... other cell), restriction enzymes (endonucleases) and DNA ligase. The use of E. coli in gene technology is well documented. Most of its DNA is in one circular chromosome, but it also has plasmids (smaller circles of DNA). These can be removed and cleaved by restriction enzymes at target sequenc ...
... other cell), restriction enzymes (endonucleases) and DNA ligase. The use of E. coli in gene technology is well documented. Most of its DNA is in one circular chromosome, but it also has plasmids (smaller circles of DNA). These can be removed and cleaved by restriction enzymes at target sequenc ...
Packet #3
... d. Spread the cells from Plate II onto a plate with LB agar with kanamycin; incubate. e. Spread the cells from Plate III onto a plate with LB agar and also onto a plate with LB agar with kanamycin; incubate. 4. A different student did the same lab experiment, but during the course of the E. coli tra ...
... d. Spread the cells from Plate II onto a plate with LB agar with kanamycin; incubate. e. Spread the cells from Plate III onto a plate with LB agar and also onto a plate with LB agar with kanamycin; incubate. 4. A different student did the same lab experiment, but during the course of the E. coli tra ...
Yeasts
... The features of the expression vectors are similar to those of yeast. The only difference is, because autonomous plasmid replication is not normally an option in commercial filamentous fungi, most vectors are designed to integrate into the fungal genome. ...
... The features of the expression vectors are similar to those of yeast. The only difference is, because autonomous plasmid replication is not normally an option in commercial filamentous fungi, most vectors are designed to integrate into the fungal genome. ...
pGLO Transformation
... Agar, which is from seaweed, polymerizes when heated to form a solid gel (very analogous to Jell-O), and functions to provide a solid support on which to culture the bacteria. Genetic Engineering The manipulation of an organism’s genetic material (DNA) by introducing or eliminating specific genes. G ...
... Agar, which is from seaweed, polymerizes when heated to form a solid gel (very analogous to Jell-O), and functions to provide a solid support on which to culture the bacteria. Genetic Engineering The manipulation of an organism’s genetic material (DNA) by introducing or eliminating specific genes. G ...
Lab 9 - Cloning GFP Lab
... Agar, which is from seaweed, polymerizes when heated to form a solid gel (very analogous to Jell-O), and functions to provide a solid support on which to culture the bacteria. Genetic Engineering The manipulation of an organism’s genetic material (DNA) by introducing or eliminating specific genes. G ...
... Agar, which is from seaweed, polymerizes when heated to form a solid gel (very analogous to Jell-O), and functions to provide a solid support on which to culture the bacteria. Genetic Engineering The manipulation of an organism’s genetic material (DNA) by introducing or eliminating specific genes. G ...
Repressor - (www.ramsey.k12.nj.us).
... A __________ is a small, circular DNA molecule that is separate from the much larger bacterial chromosome. Plasmid Restriction enzyme Genetic marker DNA fingerprint Stem cell ...
... A __________ is a small, circular DNA molecule that is separate from the much larger bacterial chromosome. Plasmid Restriction enzyme Genetic marker DNA fingerprint Stem cell ...
CHAPTER 13 * GENETIC ENGINEERING TEST REVIEW
... Eliminating an undesirable trait from a dog breed would probably require the technique of selective breeding called ___. ...
... Eliminating an undesirable trait from a dog breed would probably require the technique of selective breeding called ___. ...
Cloning :-
... 4- The vector must have at least one unique restriction endonuclease recognition site to enable DNA to be inserted during the production of recombinants. Plasmids have these features and are extensively used as vectors in cloning experiments. B- Types of vector systems:- There are two main types :1- ...
... 4- The vector must have at least one unique restriction endonuclease recognition site to enable DNA to be inserted during the production of recombinants. Plasmids have these features and are extensively used as vectors in cloning experiments. B- Types of vector systems:- There are two main types :1- ...
Lab_fundamentals
... • Carry genes that are responsible for a useful characteristic displayed by host bacterium • Survival in normally toxic concentrations of antibiotics – antibiotic resistance as a selectable marker Example: ampicillin, tetracycline, kanamycin resistant gene RP4 • At least one DNA sequence that can ac ...
... • Carry genes that are responsible for a useful characteristic displayed by host bacterium • Survival in normally toxic concentrations of antibiotics – antibiotic resistance as a selectable marker Example: ampicillin, tetracycline, kanamycin resistant gene RP4 • At least one DNA sequence that can ac ...
Genetics
... • Transposons are small pieces of DNA that move readily from one site on bacterial chromosome to another or from bacterial chromosome to plasmid. • They carry antibiotic resistance genes. • Transfer of transposons on plasmids to other bacteria by conjugation contributes to antibiotic resistance. ...
... • Transposons are small pieces of DNA that move readily from one site on bacterial chromosome to another or from bacterial chromosome to plasmid. • They carry antibiotic resistance genes. • Transfer of transposons on plasmids to other bacteria by conjugation contributes to antibiotic resistance. ...
Pre-lab 1 and Lab 1 2010 - Sonoma Valley High School
... Genetic engineering allows humans to insert human DNA into other organisms and then have these genetically modified organisms make human proteins. These proteins can be used to treat a wide variety of diseases and help millions of people. The sequence of labs in the Amgen Biotech Experience mimics t ...
... Genetic engineering allows humans to insert human DNA into other organisms and then have these genetically modified organisms make human proteins. These proteins can be used to treat a wide variety of diseases and help millions of people. The sequence of labs in the Amgen Biotech Experience mimics t ...
Document
... Selection: Isolate the brightest fluorescing cell fraction using the fluorescence activated cell sorter (FACS). Isolate the plasmids from the bright cells and re-transfect 293cos cells at a low concentration of DNA so most carry only a single plasmid and thus a single effective combination of H and ...
... Selection: Isolate the brightest fluorescing cell fraction using the fluorescence activated cell sorter (FACS). Isolate the plasmids from the bright cells and re-transfect 293cos cells at a low concentration of DNA so most carry only a single plasmid and thus a single effective combination of H and ...
Lab - Recombinant DNA Simulation
... Notice the EcoR1 cut sites are not directly across from each other on the DNA molecule. When EcoR1 makes its cuts, it leaves single-stranded “tails” on the new ends (see above). This type of end is called a STICKY END because complementary DNA sticky ends can be easily rejoined. In this activity, we ...
... Notice the EcoR1 cut sites are not directly across from each other on the DNA molecule. When EcoR1 makes its cuts, it leaves single-stranded “tails” on the new ends (see above). This type of end is called a STICKY END because complementary DNA sticky ends can be easily rejoined. In this activity, we ...
Plasmid Project due
... utilization of plasmids in recombinant DNA procedures. Recombinant DNA technology is a means by which scientists can insert genes from one species, into the DNA of another. The classic example of recombinant DNA technology is where the human insulin gene was isolated from human DNA, and was then ins ...
... utilization of plasmids in recombinant DNA procedures. Recombinant DNA technology is a means by which scientists can insert genes from one species, into the DNA of another. The classic example of recombinant DNA technology is where the human insulin gene was isolated from human DNA, and was then ins ...
A Rapid Method for the Identification of Plasmid Desoxyribonucleic
... Currently two types of rapid screening techniques for plasmid desoxyribonucleic acid (DNA) are used (1,4,5,7). One type requires little starting material, but subjects the DNA to considerable stress during lysis (5,7) or during separation of plasmid DNA from chromosomal DNA (1) and is therefore not ...
... Currently two types of rapid screening techniques for plasmid desoxyribonucleic acid (DNA) are used (1,4,5,7). One type requires little starting material, but subjects the DNA to considerable stress during lysis (5,7) or during separation of plasmid DNA from chromosomal DNA (1) and is therefore not ...
Restriction Enzyme
... • Small DNA fragment containing restriction enzyme sites • Can be attached to any DNA fragment by a ligase and cut by a particular restriction enzyme to generate specifically desired cohesive ends ...
... • Small DNA fragment containing restriction enzyme sites • Can be attached to any DNA fragment by a ligase and cut by a particular restriction enzyme to generate specifically desired cohesive ends ...
15.2 Recombinant DNA
... (Easy b/c bacteria take up DNA that’s floating around, known as transformation) ...
... (Easy b/c bacteria take up DNA that’s floating around, known as transformation) ...
What are prokaryotes?
... Sexual Reproduction Conjugation – Occurs when two bacteria join to exchange DNA – Connect through Pili ...
... Sexual Reproduction Conjugation – Occurs when two bacteria join to exchange DNA – Connect through Pili ...
Note. The efficiency of Benzonase treatment can be determined by
... 2. Gently remove transfection medium from cells 3. Very slowly add fresh culture medium to cells. Use 15-25 ml medium for a 150 mm culture dish. a. Note: cells will become dislodged very easily at this stage, so use extreme caution b. Note: if working with several plates of cells, process 1-2 at a t ...
... 2. Gently remove transfection medium from cells 3. Very slowly add fresh culture medium to cells. Use 15-25 ml medium for a 150 mm culture dish. a. Note: cells will become dislodged very easily at this stage, so use extreme caution b. Note: if working with several plates of cells, process 1-2 at a t ...
BIOL 1010
... into a bacterium, using a plasmid as a vector (see next paragraph) – the bacterium could then produce the human form of insulin. A plasmid is a circlet of DNA found in a bacterium. Plasmids are unique to bacteria and are a means by which bacteria can actually exchange genetic material. Scientists ha ...
... into a bacterium, using a plasmid as a vector (see next paragraph) – the bacterium could then produce the human form of insulin. A plasmid is a circlet of DNA found in a bacterium. Plasmids are unique to bacteria and are a means by which bacteria can actually exchange genetic material. Scientists ha ...
Freeman 1e: How we got there
... • If the gene is expressed, the presence of the foreign protein itself, as detected either by its activity or by reaction with specific antibodies, is evidence that the gene is present. However, if the gene is not expressed, its presence can be detected with a nucleic acid probe. ...
... • If the gene is expressed, the presence of the foreign protein itself, as detected either by its activity or by reaction with specific antibodies, is evidence that the gene is present. However, if the gene is not expressed, its presence can be detected with a nucleic acid probe. ...
Introduction to pGLO lab
... Then the bacteria will express the new “foreign” DNA, and the bacteria will perform new functions. ...
... Then the bacteria will express the new “foreign” DNA, and the bacteria will perform new functions. ...
Plasmid
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A plasmid is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small, circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.Plasmids are considered replicons, a unit of DNA capable of replicating autonomously within a suitable host. However, plasmids, like viruses, are not generally classified as life. Plasmids can be transmitted from one bacterium to another (even of another species) via three main mechanisms: transformation, transduction, and conjugation. This host-to-host transfer of genetic material is called horizontal gene transfer, and plasmids can be considered part of the mobilome. Unlike viruses (which encase their genetic material in a protective protein coat called a capsid), plasmids are ""naked"" DNA and do not encode genes necessary to encase the genetic material for transfer to a new host. However, some classes of plasmids encode the conjugative ""sex"" pilus necessary for their own transfer. The size of the plasmid varies from 1 to over 200 kbp, and the number of identical plasmids in a single cell can range anywhere from one to thousands under some circumstances.The relationship between microbes and plasmid DNA is neither parasitic nor mutualistic, because each implies the presence of an independent species living in a detrimental or commensal state with the host organism. Rather, plasmids provide a mechanism for horizontal gene transfer within a population of microbes and typically provide a selective advantage under a given environmental state. Plasmids may carry genes that provide resistance to naturally occurring antibiotics in a competitive environmental niche, or the proteins produced may act as toxins under similar circumstances, or allow the organism to utilize particular organic compounds that would be advantageous when nutrients are scarce.