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Extended PDF
Extended PDF

... 2011). Fiber release is mediated via an adaptor protein TapA, which forms D-amino acid-sensitive foci in the cell wall and is required for the formation of the fibers and their anchorage to the cell wall (Romero et al., 2011). D-amino acids were also found to inhibit biofilm formation by other bacte ...
Ectopic expression of beta-cytoplasmic actin
Ectopic expression of beta-cytoplasmic actin

... Multiple isoforms of actin have been described in almost all eukaryotic organisms (Gallwitz and Seidel, 1980; Fidel et al., 1988; Hirono et al., 1987; for review see Rubenstein, 1990; Herman, 1993). Humans have six actin isoforms, four of which are muscle-specific, and two are found in the cytoplasm ...
Monitoring the Disassembly of Virus
Monitoring the Disassembly of Virus

... strikingly stable under extremes of temperature,1,2 pH,2 and in different solvents.3 These render them potentially suitable for encapsulating materials such as proteins,4,5 synthetic polymers,6,7 oligonucleotides,8,9 and smaller molecules.10,11 Furthermore, their protein surface can be used to append ...
Pass the bicarb: the importance of HCO3 – for mucin release
Pass the bicarb: the importance of HCO3 – for mucin release

... CFTR dysfunction is that excessive amounts of thick, sticky mucus accumulate in affected organs. This can obstruct the organ lumen and create a permissive environment for abnormal microbial colonization, which in turn leads to chronic inflammation and tissue damage. Why CF mucus is so different from ...
Differential actin binding along the PEVK domain of skeletal muscle
Differential actin binding along the PEVK domain of skeletal muscle

... five washes of rigor buffer. Myofibrils were visualized in a laserscanning confocal microscope (Bio-Rad MRC 1024 attached to a Nikon Eclipse inverted microscope) using a 40× phase objective lens (0.75 N.A., Nikon Plan Fluor). The 488-nm line of an Argon ion laser (120-360 µW) was used. Fluorescence ...
Effect of Colchicine on Human Tissues.
Effect of Colchicine on Human Tissues.

... There were also numerous colchicine figures in the large lymphocytes, or "lymphoblasts" of the lymph nodes, the epithelium of the fallopian tubes, endometrium, and the epithelium of the renal tubules. In the latter instance, an occasional multinucleated cell could be found (fig. 6). Diagnosis: Colch ...
Auxin transport through non-hair cells sustains root
Auxin transport through non-hair cells sustains root

... Europe PMC Funders Group Author Manuscript Nat Cell Biol. Author manuscript; available in PMC 2009 July 01. Published in final edited form as: ...
Endoplasmic Reticulum-Localized Amyloid β
Endoplasmic Reticulum-Localized Amyloid β

... analysis showed that the export of Ab42 was a fast process occurring largely during the first 30 min (Figure 2B). To demonstrate that the export did not result from rupture of the microsomal membranes, the release of the ER-resident chaperone BiP was determined. BiP was not released into the superna ...
Transcription Factor Positive Regulatory Domain 4 (PRDM4) recruits Protein Arginine
Transcription Factor Positive Regulatory Domain 4 (PRDM4) recruits Protein Arginine

... indicated otherwise. ...
Regulative germ cell specification in axolotl embryos: a primitive trait
Regulative germ cell specification in axolotl embryos: a primitive trait

... urodele embryos PGCs do not develop from predetermined cells. Rather, urodele PGCs appear to develop from unspecialized mesodermal tissue that has responded to appropriate extracellular influences. Therefore the embryos of urodele and anuran amphibians have diverged in the way that they produce PGCs ...
Hairy Root Transformation Using Agrobacterium
Hairy Root Transformation Using Agrobacterium

... members of a complementary DNA (cDNA) library into tomato (Solanum spp.) roots, which were then screened for resistance to fumonisin 1 (Harvey et al., 2008). In addition, A. rhizogenes-transformed tomato roots expressing the baculovirus p35 gene were used to demonstrate the existence in plants of pr ...
Structure-Function Relationships of Stem Cell Factor
Structure-Function Relationships of Stem Cell Factor

... hematopoietic progenitor cells. The cloning of this re~eptor’‘~ and its ligand (here termed stem cell factor [SCF], but also known as c-kit ligand, mast cell growth factor and steel factor4-”); culminated decades of work begun by developmental biologists who first noted the pleiotropic effects (macr ...
2.3.1. Vector construction - Trace: Tennessee Research and
2.3.1. Vector construction - Trace: Tennessee Research and

... in microbial systems: intracellular protein-protein interactions and cell-to-cell interactions. The implementation of optical and atomic force microscopy and the methodologies developed during this study proved to be invaluable tools for investigating these systems. Identifying and characterizing pr ...
amoeba notes 14
amoeba notes 14

... The amoeba is a protozoan that belongs to the Kingdom Protista. The name amoeba comes from the Greek word “amoibe”, which means change. (Amoeba is also spelled ameba.) Protists are microscopic unicellular organisms that don't fit into the other kingdoms (fungus, plants, and animals). Some protozoans ...
(Solanum tuberosum L.) plantlets to gradient saline stress
(Solanum tuberosum L.) plantlets to gradient saline stress

... 2002; Han et al., 2014). Proline, as an important osmosis protective agent, contributes to osmotic adjustment, protecting cells from damage (Silva-Ortega et al., 2008; Abrahám et al., 2010; Hou et al., 2013; Bojorquez-quintal et al., 2014; Gupta and Huang, 2014). Salt stress also caused overproducti ...
DORSAL-VENTRAL PATTERNING AND NEURAL INDUCTION IN
DORSAL-VENTRAL PATTERNING AND NEURAL INDUCTION IN

... others (Avsian-Kretchmer & Hsueh 2004). Because BMP and Noggin share cystine knots and conserved protein folds, it has been proposed that the ligand and its antagonist may have evolved from ancestrally related proteins (Groppe et al. 2002). Chordin is a large protein of about 1000 amino acids contai ...
Mycobacterium leprae interactions with the host cell: recent
Mycobacterium leprae interactions with the host cell: recent

... to the host cell, the intracellular compartment of the organism is virtually uncharacterized. In a study by Alves et al the ST 8814 Schwannoma cell line was shown to readily phagocytize both viable, nudemouse derived M. leprae and irradiated organisms 19. This phagocytic event was shown to be depend ...
The Lyn kinase C-lobe mediates Golgi export of Lyn through
The Lyn kinase C-lobe mediates Golgi export of Lyn through

... Fig. 1. Association of Lyn with ACSL3. (A)Schematic representations of Lyn constructs, including wild-type Lyn (Lyn-wt). The Src homology (SH) domains, the N- and C-terminal lobes (N- and C-lobes) of the kinase domain, the alanine mutations of Asp346, Glu353, Asp498 and Asp499 (A in red) in the C-l ...
ABA control of plant macroelement membrane transport systems in
ABA control of plant macroelement membrane transport systems in

... Membrane ion transport systems are involved in the maintenance of cellular homeostasis during exposure to stress and ion transport activity is regulated by phosphorylation/dephosphorylation networks that respond to stress conditions. The phytohormone abscisic acid (ABA), which is produced rapidly in ...
Endodermal origin of yolk-sac-derived teratomas
Endodermal origin of yolk-sac-derived teratomas

... displaced visceral yolk sac of multipotential stem cells capable of differentiating into derivatives of all three germ layers. These stem cells might correspond to the poorly differentiated cells observed in the early stages of teratoma formation (Sobis et al. 1982). Numerous experiments indicate th ...
Archaea Topics in Biodiversity
Archaea Topics in Biodiversity

... Some Haloarchaea undergo phenotypic switching and grow as several different cell types, including thick-walled structures that are highly resistant to osmotic shock. These thickened walls permit survival under hyposaline (low salt) circumstances, but these alternative phenotypes are not actual repro ...
Abstract Panax ginseng Meyer, belonging to the genus Panax of the
Abstract Panax ginseng Meyer, belonging to the genus Panax of the

... carbohydrate-binding specificities [43]. Animal lectins were discovered before plant lectins, although many were not recognized as carbohydrate binding proteins for many years after first being reported [44]. Although plant and animal lectins do not have homologous primary structures, they have sim ...
PDF - Oxford Academic - Oxford University Press
PDF - Oxford Academic - Oxford University Press

... GUS coloration restricted to the endodermal and pericycle cell layers was observed from the hypocotyl–root junction throughout the primary root (Fig. 1D). Despite various attempts, we were unable to detect any staining in either lateral roots or other plant tissues, namely flowers and mature green o ...
A Point Mutation in the Ethylene-Inducing Xylanase Elicitor Inhibits
A Point Mutation in the Ethylene-Inducing Xylanase Elicitor Inhibits

... (Fig. 1C). These data show that the addition of an enzyme (XynI) with similar enzymatic activity as EIX or XynII does not influence the induction of ethylene biosynthesis by EIX or XynII. Thus, the xylanase activity of EIX appears to be unrelated to the elicitation process. However, differences in s ...
PDF
PDF

... Next we analyzed constitutive germ cell apoptosis in lin35(n745) animals. Germ cells undergoing apoptosis become refractile and are engulfed by the sheath cells, which are part of the somatic gonad and which express the scavenger receptor-like protein CED-1 on their cell surface (Zhou et al., 2001). ...
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Cytokinesis



Cytokinesis (cyto- + kinesis) is the process during cell division in which the cytoplasm of a single eukaryotic cell is divided to form two daughter cells. It usually initiates during the early stages of mitosis, and sometimes meiosis, splitting a mitotic cell in two, to ensure that chromosome number is maintained from one generation to the next. After cytokinesis two (daughter) cells will be formed that are exact copies of the (parent) original cell. After cytokinesis, each daughter cell is in the interphase portion of the cell cycle. In animal cells, one notable exception to the normal process of cytokinesis is oogenesis (the creation of an ovum in the ovarian follicle of the ovary), where the ovum takes almost all the cytoplasm and organelles, leaving very little for the resulting polar bodies, which then die. Another form of mitosis without cytokinesis occurs in the liver, yielding multinucleate cells. In plant cells, a dividing structure known as the cell plate forms within the centre of the cytoplasm and a new cell wall forms between the two daughter cells.Cytokinesis is distinguished from the prokaryotic process of binary fission.
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