A Review of Centriole Activity, and Wrongful Activity, during Cell
... Following centriole duplication and separation into two pair, the younger pair moves around the nucleus to the opposite side. While this is occurring, the nuclear membrane begins to soften. The centrioles at the opposite sides of the nucleus then begin to pull the nucleus apart. As the nucleus is be ...
... Following centriole duplication and separation into two pair, the younger pair moves around the nucleus to the opposite side. While this is occurring, the nuclear membrane begins to soften. The centrioles at the opposite sides of the nucleus then begin to pull the nucleus apart. As the nucleus is be ...
MAPK Phosphatase AP2C3 Induces Ectopic Proliferation of
... transcription factors that are successively ushering cells though the steps of stomata lineage: SPEECHLESS (SPCH) - initiation;, MUTE - proliferation; and FAMA - stomata differentiation [13,14,15,16]. Phosphorylation of SPCH by MPK6 was shown to control the entry into stomata lineage and to connect ...
... transcription factors that are successively ushering cells though the steps of stomata lineage: SPEECHLESS (SPCH) - initiation;, MUTE - proliferation; and FAMA - stomata differentiation [13,14,15,16]. Phosphorylation of SPCH by MPK6 was shown to control the entry into stomata lineage and to connect ...
Remodeling of endosomes during lysosome biogenesis involves
... for 30 minutes. Cells were then fixed in 1% glutaraldehyde. HRP was revealed by a DAB (Sigma, St Louis, MO) reaction as described previously (Desjardins et al., 1994). Cells were post-fixed in 1% OsO4, dehydrated in alcohol, processed for flat embedding in Epon 812 and observed at the Zeiss CEM 902 ...
... for 30 minutes. Cells were then fixed in 1% glutaraldehyde. HRP was revealed by a DAB (Sigma, St Louis, MO) reaction as described previously (Desjardins et al., 1994). Cells were post-fixed in 1% OsO4, dehydrated in alcohol, processed for flat embedding in Epon 812 and observed at the Zeiss CEM 902 ...
Keystone Exam Study Guide
... BIO.A.4.1.3 Describe how membrane-bound cellular organelles (e.g., endoplasmic reticulum, Golgi apparatus) facilitate the transport of materials within a cell. ...
... BIO.A.4.1.3 Describe how membrane-bound cellular organelles (e.g., endoplasmic reticulum, Golgi apparatus) facilitate the transport of materials within a cell. ...
Studies of the cat`s medial interlaminar nucleus
... of 15 normal adult cats with electrophysiological and anatomical techniques. Autoradiographs processed from cats t h a t had one eye injected with tritiated fucose and proline indicate that MIN is composed of at least two laminae, one for each eye. The area which receives input from the ipsilateral ...
... of 15 normal adult cats with electrophysiological and anatomical techniques. Autoradiographs processed from cats t h a t had one eye injected with tritiated fucose and proline indicate that MIN is composed of at least two laminae, one for each eye. The area which receives input from the ipsilateral ...
"VESICLE IN A BASKET" A Morphological Study of
... on the general role of bristles in the mechanism of membrane vesiculation . Bowers (8) suggested further that the coatings may act as the binding sites for protein. It seems, however, that the binding of protein occurs first on the outer surface of the membrane, even though the bristles are located ...
... on the general role of bristles in the mechanism of membrane vesiculation . Bowers (8) suggested further that the coatings may act as the binding sites for protein. It seems, however, that the binding of protein occurs first on the outer surface of the membrane, even though the bristles are located ...
Analysis of the Golgi Apparatus in Arabidopsis Seed
... destination of cargo has little effect on the location of the Golgi stack within the cell. ...
... destination of cargo has little effect on the location of the Golgi stack within the cell. ...
Plant Cell, 20, 1623-1638. - McGill Biology
... destination of cargo has little effect on the location of the Golgi stack within the cell. ...
... destination of cargo has little effect on the location of the Golgi stack within the cell. ...
Branching of Sensory and Sympathetic Neurites in vitro Is Inhibited
... aspects of neuronal form (Sotelo and Arsenio-Nunes, 1976; Van der Loos, 1965). It is clear, then, from a variety of studies that intrinsic regulatory factors contribute to the neuritic branching patterns of different neuronal types. In this paper we present evidence that the microtubular organizatio ...
... aspects of neuronal form (Sotelo and Arsenio-Nunes, 1976; Van der Loos, 1965). It is clear, then, from a variety of studies that intrinsic regulatory factors contribute to the neuritic branching patterns of different neuronal types. In this paper we present evidence that the microtubular organizatio ...
Golgi Regeneration after Brefeldin A Treatment in
... investigation Golgi regeneration in synchronized tobacco BY-2 cells was followed by electron microscopy and by the immunofluorescence detection of ARF1, which localizes to the rims of Golgi cisternae and serves as an indicator of COPI vesiculation. Beginning as clusters of vesicles that are COPI pos ...
... investigation Golgi regeneration in synchronized tobacco BY-2 cells was followed by electron microscopy and by the immunofluorescence detection of ARF1, which localizes to the rims of Golgi cisternae and serves as an indicator of COPI vesiculation. Beginning as clusters of vesicles that are COPI pos ...
Thesis-1972D-H792p
... It should be pointed out that compounds such as penicillin, vancomycin, or D-amino acids which inhibit division activity and cell wall synthesis do not impair DNA synthesis (Grula et al., 1968), Other investigators, (Weidel, Frank, and Martin, 1964) have equated inhibition of cell division, in the p ...
... It should be pointed out that compounds such as penicillin, vancomycin, or D-amino acids which inhibit division activity and cell wall synthesis do not impair DNA synthesis (Grula et al., 1968), Other investigators, (Weidel, Frank, and Martin, 1964) have equated inhibition of cell division, in the p ...
Embryonic Stem Cell Culture Conditions Support Distinct States
... therefore utilized mice from an F1 hybrid genetic background, reported to generate chimeras with high efficiency based on hybrid vigor (Eggan et al., 2001). We derived F1 129S2;C57BL/6N hybrid ESC lines in conventional serum/LIF (serum) culture conditions, as well as 2i/LIF (2i) and KOSR/LIF (KOSR). ...
... therefore utilized mice from an F1 hybrid genetic background, reported to generate chimeras with high efficiency based on hybrid vigor (Eggan et al., 2001). We derived F1 129S2;C57BL/6N hybrid ESC lines in conventional serum/LIF (serum) culture conditions, as well as 2i/LIF (2i) and KOSR/LIF (KOSR). ...
Early development of the zebrafish pronephros and analysis of
... more laterally, adjacent to the anterior tip of the pronephric duct (Fig. 2E). In cross section, midline cells appear to be enclosed by a basement membrane or capsule, while lateral cells are beginning to take on the appearance of the pronephric tubule (Fig. 2E; see below). The morphological distinc ...
... more laterally, adjacent to the anterior tip of the pronephric duct (Fig. 2E). In cross section, midline cells appear to be enclosed by a basement membrane or capsule, while lateral cells are beginning to take on the appearance of the pronephric tubule (Fig. 2E; see below). The morphological distinc ...
western blotting - New England Biolabs GmbH
... and positive (TPA-treated) Jurkat lysate, as well as purified recombinant tyrosine-phosphorylated proteins. The importance of using a well-validated antibody and the inclusion of proper experimental controls is demonstrated by the cross-reactivity of the alternate providers’ antibodies in both negat ...
... and positive (TPA-treated) Jurkat lysate, as well as purified recombinant tyrosine-phosphorylated proteins. The importance of using a well-validated antibody and the inclusion of proper experimental controls is demonstrated by the cross-reactivity of the alternate providers’ antibodies in both negat ...
The Functional Influence of Burst and Tonic Firing Mode on Synaptic
... diminished amplitude. The number above each trace is the average frequency of action potential discharge. Ba, The number of single-spike IPSPs that contribute to the peak amplitude increases with increasing rate of presynaptic action potential generation. Bb, The peak amplitude of summated IPSPs inc ...
... diminished amplitude. The number above each trace is the average frequency of action potential discharge. Ba, The number of single-spike IPSPs that contribute to the peak amplitude increases with increasing rate of presynaptic action potential generation. Bb, The peak amplitude of summated IPSPs inc ...
Quantifying bacteria internalization by macrophage
... because GBS cytoskeletal degradation was hindered. The image analysis together with cell assays showed that GBS can evade macrophage antimicrobicidal defenses by cleaving several structural and regulatory cytoskeletal proteins, which leads to cytoskeleton disruption. The mechanism for this disruptio ...
... because GBS cytoskeletal degradation was hindered. The image analysis together with cell assays showed that GBS can evade macrophage antimicrobicidal defenses by cleaving several structural and regulatory cytoskeletal proteins, which leads to cytoskeleton disruption. The mechanism for this disruptio ...
zChap02_140901 - Online Open Genetics
... The ends of linear chromosomes present a problem – at each end one strand cannot be completely replicated because there is no primer to extend. Although the loss of such a small sequence would not be a problem, the continued rounds of replication would result in the continued loss of sequence from t ...
... The ends of linear chromosomes present a problem – at each end one strand cannot be completely replicated because there is no primer to extend. Although the loss of such a small sequence would not be a problem, the continued rounds of replication would result in the continued loss of sequence from t ...
Bacillus anthracis produces membrane-derived vesicles containing biologically active toxins
... both pathogenic and nonpathogenic, Gram-negative bacteria, and the role of outer membrane vesicles in pathogenesis are of great interest. Recently, eukaryotic pathogens, such as Cryptococcus neoformans, have been found to release virulence factors in vesicles, suggesting that this is a widely used s ...
... both pathogenic and nonpathogenic, Gram-negative bacteria, and the role of outer membrane vesicles in pathogenesis are of great interest. Recently, eukaryotic pathogens, such as Cryptococcus neoformans, have been found to release virulence factors in vesicles, suggesting that this is a widely used s ...
Vacuolar transporters and their essential role in plant metabolism
... It appears that vacuoles are organelles which fulfil highly specialized functions depending on tissue and cell type, and their developmental stage. All vacuoles seem to contain the vacuolar-type H+-ATPase (V-ATPase; Sze et al., 1999), H+-translocating inorganic pyrophosphatase (V-PPase; Maeshima, 20 ...
... It appears that vacuoles are organelles which fulfil highly specialized functions depending on tissue and cell type, and their developmental stage. All vacuoles seem to contain the vacuolar-type H+-ATPase (V-ATPase; Sze et al., 1999), H+-translocating inorganic pyrophosphatase (V-PPase; Maeshima, 20 ...
The Development of Radial and Biradial Symmetry: The Evolution of
... other metazoans appear to display these developmental features (Goldstein and Freeman, 1997). It is interesting to note, however, that the oral pole of adult cnidarians (hydrozoans) is generated from the posterior pole of the planula larva. This seems to indicate that there is an "inversion" of axia ...
... other metazoans appear to display these developmental features (Goldstein and Freeman, 1997). It is interesting to note, however, that the oral pole of adult cnidarians (hydrozoans) is generated from the posterior pole of the planula larva. This seems to indicate that there is an "inversion" of axia ...
High-Performance Exosome Purification
... sample preparation indicate that density gradient is an impor tant step in isolating exosomes. When ultracentrifugation and pelleting are used alone, without a density gradient, larger species and cellular debris are removed from the exosome sample. However, protein and small macromolecule impuritie ...
... sample preparation indicate that density gradient is an impor tant step in isolating exosomes. When ultracentrifugation and pelleting are used alone, without a density gradient, larger species and cellular debris are removed from the exosome sample. However, protein and small macromolecule impuritie ...
HESI/ILSI Health and Environmental Sciences Institute
... In silico modeling in drug safety/mechanisms In vitro cell biology of IKr Stem cell research and more Genetic screening/other biomarkers ...
... In silico modeling in drug safety/mechanisms In vitro cell biology of IKr Stem cell research and more Genetic screening/other biomarkers ...
The Development of Radial and Biradial Symmetry: The Evolution of
... other metazoans appear to display these developmental features (Goldstein and Freeman, 1997). It is interesting to note, however, that the oral pole of adult cnidarians (hydrozoans) is generated from the posterior pole of the planula larva. This seems to indicate that there is an "inversion" of axia ...
... other metazoans appear to display these developmental features (Goldstein and Freeman, 1997). It is interesting to note, however, that the oral pole of adult cnidarians (hydrozoans) is generated from the posterior pole of the planula larva. This seems to indicate that there is an "inversion" of axia ...
Cytokinesis
Cytokinesis (cyto- + kinesis) is the process during cell division in which the cytoplasm of a single eukaryotic cell is divided to form two daughter cells. It usually initiates during the early stages of mitosis, and sometimes meiosis, splitting a mitotic cell in two, to ensure that chromosome number is maintained from one generation to the next. After cytokinesis two (daughter) cells will be formed that are exact copies of the (parent) original cell. After cytokinesis, each daughter cell is in the interphase portion of the cell cycle. In animal cells, one notable exception to the normal process of cytokinesis is oogenesis (the creation of an ovum in the ovarian follicle of the ovary), where the ovum takes almost all the cytoplasm and organelles, leaving very little for the resulting polar bodies, which then die. Another form of mitosis without cytokinesis occurs in the liver, yielding multinucleate cells. In plant cells, a dividing structure known as the cell plate forms within the centre of the cytoplasm and a new cell wall forms between the two daughter cells.Cytokinesis is distinguished from the prokaryotic process of binary fission.