p23 and organization of the Golgi apparatus
... may act as cargo adaptors, and specifically interact with cargo molecules on transport vesicles (Schimmöller et al., 1995; Belden and Barlowe, 1996). Until now, no specific association between a p24 protein and a cargo molecule has been reported. However, it is possible that p24-cargo interactions a ...
... may act as cargo adaptors, and specifically interact with cargo molecules on transport vesicles (Schimmöller et al., 1995; Belden and Barlowe, 1996). Until now, no specific association between a p24 protein and a cargo molecule has been reported. However, it is possible that p24-cargo interactions a ...
Molecular Genetic Analysis of Phototropism in
... phototropins (reviewed by Christie 2007). Each FMN molecule associates with the LOV domains non-covalently in a dark state. Blue light absorption then causes the formation of a transient covalent adduct between the FMN and a critical cysteine residue in the LOV domain. This binding causes a conforma ...
... phototropins (reviewed by Christie 2007). Each FMN molecule associates with the LOV domains non-covalently in a dark state. Blue light absorption then causes the formation of a transient covalent adduct between the FMN and a critical cysteine residue in the LOV domain. This binding causes a conforma ...
Maternal macho-1 is an intrinsic factor that makes cell
... notochord fate (induced fate). However, another daughter cell follows a muscle or nerve cord fate (default fate) (Kim and Nishida, 1999; Minokawa et al., 2001). (4) Endoderm blastomeres are the inducers, and FGF signaling mediates the induction. Mesenchyme and notochord are induced by the same FGF s ...
... notochord fate (induced fate). However, another daughter cell follows a muscle or nerve cord fate (default fate) (Kim and Nishida, 1999; Minokawa et al., 2001). (4) Endoderm blastomeres are the inducers, and FGF signaling mediates the induction. Mesenchyme and notochord are induced by the same FGF s ...
single cotyledon (sic) mutants of pea and their significance in
... embryo has only a single plane of symmetry, in contrast to many dicots. To clarify this point, a dorsal-ventral concept from animal embryology was adopted to describe the morphology of the pea embryo. The ventral side was where the apical meristem was situated, which was close to the testa in vivo. ...
... embryo has only a single plane of symmetry, in contrast to many dicots. To clarify this point, a dorsal-ventral concept from animal embryology was adopted to describe the morphology of the pea embryo. The ventral side was where the apical meristem was situated, which was close to the testa in vivo. ...
Ascorbic acid: metabolism and functions of a multi
... plants. Its biosynthetic pathway via GDP-D-mannose and L-galactose, which was proposed only recently, is now supported by molecular genetic evidence from Arabidopsis thahna and transgenic potato plants. Except for the last step (which is located on the inner mitochondrial membrane) the pathway is cy ...
... plants. Its biosynthetic pathway via GDP-D-mannose and L-galactose, which was proposed only recently, is now supported by molecular genetic evidence from Arabidopsis thahna and transgenic potato plants. Except for the last step (which is located on the inner mitochondrial membrane) the pathway is cy ...
–plasma-membrane junctions by Kv2.1 Induction of stable ER potassium channels
... microscopy. HEK cells were either mock transfected (no plasmid DNA) or transfected with a construct encoding Kv2.1 that had an extracellular hemagglutinin tag (Kv2.1–HA), and then fixed with glutaraldehyde. Cells were then labeled with a primary antibody against HA and secondary antibodies conjugate ...
... microscopy. HEK cells were either mock transfected (no plasmid DNA) or transfected with a construct encoding Kv2.1 that had an extracellular hemagglutinin tag (Kv2.1–HA), and then fixed with glutaraldehyde. Cells were then labeled with a primary antibody against HA and secondary antibodies conjugate ...
Supplementary Data - Institute of Cancer Research
... Decision-making in the immune system is based on correctly assessing the level of threat. The innate immune system in particular is triggered when host pattern recognition receptors (PRRs) encounter microbe-associated molecular patterns (MAMPs) 1, 2. MAMPs are typically conserved structural motifs ...
... Decision-making in the immune system is based on correctly assessing the level of threat. The innate immune system in particular is triggered when host pattern recognition receptors (PRRs) encounter microbe-associated molecular patterns (MAMPs) 1, 2. MAMPs are typically conserved structural motifs ...
Detection, Characterization, and Bioavailability of Membrane
... ethanolamine from Amersham Corp., Arlington Heights, IL; and other reagents from Sigma Chemical Co., St. Louis, MO. RBC preparation. RBC were obtained from heparinized blood from normal donors, three patients with sickle cell anemia, a high reticulocyte control patient with hereditary spherocytosis ...
... ethanolamine from Amersham Corp., Arlington Heights, IL; and other reagents from Sigma Chemical Co., St. Louis, MO. RBC preparation. RBC were obtained from heparinized blood from normal donors, three patients with sickle cell anemia, a high reticulocyte control patient with hereditary spherocytosis ...
DC-derived exosomes bear molecules involved in
... or if they are internalized and processed, as occurs with vesicles derived from apoptotic cells 2-5. Herein we demonstrate that exosomes are internalized efficiently by DC. Targeting of exosomes to DC depends on ligands on the exosome and DC surface and is independent of complement factors. Once int ...
... or if they are internalized and processed, as occurs with vesicles derived from apoptotic cells 2-5. Herein we demonstrate that exosomes are internalized efficiently by DC. Targeting of exosomes to DC depends on ligands on the exosome and DC surface and is independent of complement factors. Once int ...
CalS7 encodes a callose synthase responsible for callose
... Upon dissolution of the callose and removal of the middle lamella cell wall material, a plate structure with widened pores is developed. According to this model, the amount of deposited callose may govern the final physical size of the sieve pores. Throughout sieve pore development, the ER remains p ...
... Upon dissolution of the callose and removal of the middle lamella cell wall material, a plate structure with widened pores is developed. According to this model, the amount of deposited callose may govern the final physical size of the sieve pores. Throughout sieve pore development, the ER remains p ...
Structural Investigations of Liposomes: Effect of Phospholipid
... the phosopholipid can be used to predict which structures will form (Israelachvili 1991). These parameters most efficiently predict the structures of unilamellar, single component supramolecular structures (Israelachvili 1991). In a multilamellar structure the interaction of head groups between sepa ...
... the phosopholipid can be used to predict which structures will form (Israelachvili 1991). These parameters most efficiently predict the structures of unilamellar, single component supramolecular structures (Israelachvili 1991). In a multilamellar structure the interaction of head groups between sepa ...
A Study of Specificity of Cores for Group D Streptococci
... protrusions from the main cell body (Cohen et al. 1968); in contrast, in the micrographs of Corfield & Smith (1968), intracellular 'microtubular ' structures are hollow tubular invaginations of the cytoplasmic membrane of not more than about 0 - 2pm. in length and can be very numerous in some cells. ...
... protrusions from the main cell body (Cohen et al. 1968); in contrast, in the micrographs of Corfield & Smith (1968), intracellular 'microtubular ' structures are hollow tubular invaginations of the cytoplasmic membrane of not more than about 0 - 2pm. in length and can be very numerous in some cells. ...
assembly of integral membrane proteins from the periplasm into the
... unfolded OMPs on affinity columns (Chen and Henning, 1996). E. coli cells lacking the skp gene display reduced levels of OmpA, OmpC, OmpF, and LamB in the OM (Chen and Henning, 1996; Missiakas et al., 1996), a phenotype which resembles that of surA mutants (Missiakas et al., 1996; Rouvière and Gross ...
... unfolded OMPs on affinity columns (Chen and Henning, 1996). E. coli cells lacking the skp gene display reduced levels of OmpA, OmpC, OmpF, and LamB in the OM (Chen and Henning, 1996; Missiakas et al., 1996), a phenotype which resembles that of surA mutants (Missiakas et al., 1996; Rouvière and Gross ...
Micronuclei and the Cytoplasm of Growing Tetrahymena Contain a
... protozoan, Tetrahymena. One system, which we will refer to as "transcription-related" acetylation, is postsynthetic and affects all of the inner (non-H1) histones in transcriptionally active macronuclei. This system does not appear to exist in transcriptionally inactive micronuclei (Vavra et al., 19 ...
... protozoan, Tetrahymena. One system, which we will refer to as "transcription-related" acetylation, is postsynthetic and affects all of the inner (non-H1) histones in transcriptionally active macronuclei. This system does not appear to exist in transcriptionally inactive micronuclei (Vavra et al., 19 ...
AcmA of Lactococcus lactis is an N-acetylglucosaminidase
... of this peptidoglycan hydrolase that is essential for flagellar rod formation [10]. In the sequence of the genome of L. lactis IL1403 genes putatively encoding cell wall hydrolases with an active site homologous to that of AcmA are present (acmB, acmC and acmD). AcmD, like AcmA, contains three LysM d ...
... of this peptidoglycan hydrolase that is essential for flagellar rod formation [10]. In the sequence of the genome of L. lactis IL1403 genes putatively encoding cell wall hydrolases with an active site homologous to that of AcmA are present (acmB, acmC and acmD). AcmD, like AcmA, contains three LysM d ...
Cornell University, 1989 M.S. Environmental Engineering Stanford University, 1990
... I am indebted to my other comn-ittee members, Phil Gschwend, Jim McCarthy, and Rob Olson for their patience and devotion to my work over these many years. Through them, I have been able to gain many different insights into the world of oceanography, and science in general. Over my final year, I have ...
... I am indebted to my other comn-ittee members, Phil Gschwend, Jim McCarthy, and Rob Olson for their patience and devotion to my work over these many years. Through them, I have been able to gain many different insights into the world of oceanography, and science in general. Over my final year, I have ...
Novel Mechanism of PTEN Regulation by Its
... Exponentially growing cells were transformed with expression vectors (10 g of DNA) by electroporation and selected on plates for 7–10 days in HL5 containing 10 g/ml G418. When foci were visible, cells were harvested and observed for expression by fluorescence microscopy and immunoblotting. Chemota ...
... Exponentially growing cells were transformed with expression vectors (10 g of DNA) by electroporation and selected on plates for 7–10 days in HL5 containing 10 g/ml G418. When foci were visible, cells were harvested and observed for expression by fluorescence microscopy and immunoblotting. Chemota ...
Improper chromosome synapsis is associated with
... their homologous partners and undergo homologous recombination. In many organisms, homologous chromosome synapsis is directly preceded by a profound nuclear reorganization, generation of the telomere bouquet (Zickler and Kleckner 1998). Although the telomere bouquet has been postulated to facilitate ...
... their homologous partners and undergo homologous recombination. In many organisms, homologous chromosome synapsis is directly preceded by a profound nuclear reorganization, generation of the telomere bouquet (Zickler and Kleckner 1998). Although the telomere bouquet has been postulated to facilitate ...
Induction of stable ER–plasma-membrane junctions by Kv2.1
... microscopy. HEK cells were either mock transfected (no plasmid DNA) or transfected with a construct encoding Kv2.1 that had an extracellular hemagglutinin tag (Kv2.1–HA), and then fixed with glutaraldehyde. Cells were then labeled with a primary antibody against HA and secondary antibodies conjugate ...
... microscopy. HEK cells were either mock transfected (no plasmid DNA) or transfected with a construct encoding Kv2.1 that had an extracellular hemagglutinin tag (Kv2.1–HA), and then fixed with glutaraldehyde. Cells were then labeled with a primary antibody against HA and secondary antibodies conjugate ...
Fetal Endoderm Primarily Holds the Temporal and Positional
... coated with 1% gelatine and 2% paraformaldehyde. The presence of specific proteins was analyzed by indirect immunofluoreseent staining. Cryosecti0ns were incubated 2 h at room temperature with mouse monocional antibodies against rat lactase (Quaroni and Isselbacher, 1985) and suerase (Hauri et al., ...
... coated with 1% gelatine and 2% paraformaldehyde. The presence of specific proteins was analyzed by indirect immunofluoreseent staining. Cryosecti0ns were incubated 2 h at room temperature with mouse monocional antibodies against rat lactase (Quaroni and Isselbacher, 1985) and suerase (Hauri et al., ...
Viroids - general characteristics and examples of diseases caused
... RNAs to be discovered in nature. These are the smallest known infectious agents. Potato spindle tuber viroid was the first viroid reported, and it is widely prevalent in different potato growing areas. Citrus exocortis viroid is wide spread in citrus production areas where trifoliate ornage(Poncirus ...
... RNAs to be discovered in nature. These are the smallest known infectious agents. Potato spindle tuber viroid was the first viroid reported, and it is widely prevalent in different potato growing areas. Citrus exocortis viroid is wide spread in citrus production areas where trifoliate ornage(Poncirus ...
McClay et al.
... nearest the vegetative pole possesses the greatest potentiality to bring development completely to the pluteus stage. It is the ‘priority region’ where differentiation begins. And when differentiation has begun, ‘from this center all other regions are determined in their role by a regulatory action” ...
... nearest the vegetative pole possesses the greatest potentiality to bring development completely to the pluteus stage. It is the ‘priority region’ where differentiation begins. And when differentiation has begun, ‘from this center all other regions are determined in their role by a regulatory action” ...
Print - Circulation Research
... these functional alterations which showed greater elevation in LV end diastolic pressure (Online Table II) and reduced myocardial contractility as assessed by ⫹dP/dtmax (Figure 3D; Online Table II) and ⫺dP/dtmin (Figure 3E; Online Table II) in banded PI3K␥KO mice. The early and marked decompensation ...
... these functional alterations which showed greater elevation in LV end diastolic pressure (Online Table II) and reduced myocardial contractility as assessed by ⫹dP/dtmax (Figure 3D; Online Table II) and ⫺dP/dtmin (Figure 3E; Online Table II) in banded PI3K␥KO mice. The early and marked decompensation ...
Cell fate specification and polarisation in mouse preimplantation
... This thesis may be made available for consultation within the University Library and may be photocopied or lent to other libraries for the purposes of consultation with effect from ………………….. Signed on behalf of the Faculty of Science …………………….. ...
... This thesis may be made available for consultation within the University Library and may be photocopied or lent to other libraries for the purposes of consultation with effect from ………………….. Signed on behalf of the Faculty of Science …………………….. ...
Association of FMRP with Ribosomal Precursor Particles in the
... since in cells from fragile X patients, lacking FMRP, a normal transport of ribosomes from the nucleus to the cytoplasm occurs. On the other hand, the RNA binding properties of FMRP and the in vitro association with ribosomes led to different proposals for a theoretical function for FMRP. One possib ...
... since in cells from fragile X patients, lacking FMRP, a normal transport of ribosomes from the nucleus to the cytoplasm occurs. On the other hand, the RNA binding properties of FMRP and the in vitro association with ribosomes led to different proposals for a theoretical function for FMRP. One possib ...
Cytokinesis
Cytokinesis (cyto- + kinesis) is the process during cell division in which the cytoplasm of a single eukaryotic cell is divided to form two daughter cells. It usually initiates during the early stages of mitosis, and sometimes meiosis, splitting a mitotic cell in two, to ensure that chromosome number is maintained from one generation to the next. After cytokinesis two (daughter) cells will be formed that are exact copies of the (parent) original cell. After cytokinesis, each daughter cell is in the interphase portion of the cell cycle. In animal cells, one notable exception to the normal process of cytokinesis is oogenesis (the creation of an ovum in the ovarian follicle of the ovary), where the ovum takes almost all the cytoplasm and organelles, leaving very little for the resulting polar bodies, which then die. Another form of mitosis without cytokinesis occurs in the liver, yielding multinucleate cells. In plant cells, a dividing structure known as the cell plate forms within the centre of the cytoplasm and a new cell wall forms between the two daughter cells.Cytokinesis is distinguished from the prokaryotic process of binary fission.