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The COPI Complex Functions in Nuclear Envelope Breakdown and
The COPI Complex Functions in Nuclear Envelope Breakdown and

... 2003). Despite the continuity at each nuclear pore between the inner and outer nuclear membranes, a unique profile of proteins are anchored in the inner nuclear membrane through interactions with proteins located at the peripheral nuclear lamina and chromatin (Burke and Ellenberg, 2002). In metazoan ...
Botanical Gazette
Botanical Gazette

... Please contact the publisher regarding any further use of this work. Publisher contact information may be obtained at http://www.jstor.org/journals/ucpress.html. Each copy of any part of a JSTOR transmission must contain the same copyright notice that appears on the screen or printed page of such tr ...
Expression and Endocytosis of Lysosomal
Expression and Endocytosis of Lysosomal

... of synaptophysin could be detected, indicating that AGA is not transported to the nerve terminals (data not shown). However, when the expression pattern of AGA was monitored at the different chase periods, the complete lysosomal-like staining pattern appeared as late as 10 hr after the protein synth ...
Chapter 2 - William Stallings, Data and Computer
Chapter 2 - William Stallings, Data and Computer

... • consider ATM at 150Mbps • takes ~2.8x10-6 seconds to insert single cell • time to traverse network depends on propagation delay and switching delay • assume propagation at two-thirds speed of light • if source and destination on opposite sides of USA, propagation time ~ 48x10-3 seconds • given imp ...
BYSTANDER HELP IN PRIMARY IMMUNE RESPONSES IN
BYSTANDER HELP IN PRIMARY IMMUNE RESPONSES IN

... titer of Fl-specific antibody in B10, B10.A, and BALB.B mice (Fig. 2). Both antigens must be injected in the same anatomical location, but there is no requirement for mixing of the antigens before immunization . Separate intraperitoneal injections of each antigen will induce hapten-specific antibody ...
Nuclear Synthesis of Cytoplasmic Ribonucleic Acid
Nuclear Synthesis of Cytoplasmic Ribonucleic Acid

... radioactivity of RNA that is observed within either nucleated or enucleated amoebae. It is obvious that a clear distinction must be made between the activities of the amoeba itself and the activities of the organisms (or possibly even partially digested organisms) within the amoeba food vacuoles or ...
Clinical Implications of Intestinal Stem Cell Markers in Colorectal
Clinical Implications of Intestinal Stem Cell Markers in Colorectal

... Lgr5 expressing cells are proposed to mark actively cycling stem cells exerting a homeostatic role in the small intestine of mice.18 Furthermore, spheroid cultures derived from human primary tumors are enriched for LGR5 expression, and the receptor has been suggested to be a selective cancer stem ce ...
Progressive lineage analysis by cell sorting and culture identifies
Progressive lineage analysis by cell sorting and culture identifies

... mesoderm was induced in the embryoid body with a time schedule similar to that of actual embryo. In some studies, the microenvironment in the embryoid body for the specification to blood cells has been successfully substituted using feeder cell lines (Gutierrez-Ramos and Palacios, 1992; Nakano et al ...
Resident and infiltrating immune cells in the uveal tract in the
Resident and infiltrating immune cells in the uveal tract in the

... Purpose. To investigate the dynamics of resident and infiltrating immune cells in the choroid and iris during the early and late stages of experimental autoimmune uveitis (EAU) in Lewis rats. Methods. Uveoretinitis was induced by footpad injection of crude retinal extract and complete Freund's adjuv ...
Reciprocal signaling between Drosophila epidermal muscle
Reciprocal signaling between Drosophila epidermal muscle

... target EMA cells; mutant embryos in which these cells are missing or disorganized exhibit an aberrant pattern of somatic muscles (Volk and VijayRaghavan, 1994). A key gene in the proper differentiation of the EMA cells is stripe (De la Pompa et al., 1989). Embryos that carry a stripe severe mutation ...
Formation of Earlywood, Latewood, and Heartwood Regulation of
Formation of Earlywood, Latewood, and Heartwood Regulation of

... • The biosynthetic pathway of cytokinins is not fully elucidated, but it has been suggested that they are synthesized by the root apical meristem, and transported to the stem through vascular bundles. • Several kinds of cytokinins were found in the roots of Pseudotsuga menziesii, while in the cambia ...
Arabidopsis root K - Journal of Cell Science
Arabidopsis root K - Journal of Cell Science

... cation influx, the effect of ROS on cation efflux remains poorly studied. Hydroxyl radical (HR)-activated K+-efflux conductance has been observed alongside the activation of Ca2+ channels (Demidchik et al., 2003), but has not been investigated in detail; the singlechannel characteristics, genetic ba ...
ER and vacuoles: never been closer
ER and vacuoles: never been closer

... Relatively little is known about the biogenesis of vacuoles in plants. Even the donor membrane from where newly formed vacuoles originate from is unclear. The model that boasts most of the credits in text books suggests that newly formed lytic vacuoles in root-tip cells originate from post-Golgi-der ...
Expression of Nuclear Lamin A and Muscle
Expression of Nuclear Lamin A and Muscle

... in sample buffer were diluted 1:3 in 5 mM NaOH, and adjusted to 0.1 M NaCI. Two volumes of cold 100% ethanol were added, and the mixture incubated at 4°C overnight. The precipitated DNA was washed two times with 70% ethanol and air dried. To the dried pellets, equal volumes of 5 mM NaOH and i N HCIO ...
impression cytologic analysis of superior limbic keratoconjunctivitis
impression cytologic analysis of superior limbic keratoconjunctivitis

...  Extensive nuclear pyknosis  Condensation of masses of chromatin  The shrinkage of the nuclear envelope from its surrounding cytoplasm ...
Conservation of inner nuclear membrane targeting sequences in
Conservation of inner nuclear membrane targeting sequences in

... pressed in bacteria plasmids encoding ΔIBB-importin α1 and GSTtagged Pom121NLS (residues 291–320), and captured a stoichiomet­ ric complex of the two proteins on glutathione beads. Coexpression prevented proteolytic degradation of Pom121NLS291-320 and was instrumental in purifying milligram quantity ...
Nucleation and Dynamics of Golgi-derived Microtubules
Nucleation and Dynamics of Golgi-derived Microtubules

Differential Expression Control and Polarized Distribution of Plasma
Differential Expression Control and Polarized Distribution of Plasma

... cell (Fig. 1F, H, I). Although in non-dividing cells the fluorescence of GFP–SYP132 was only detected on the PM, in dividing cells its fluorescence was also observed in cytosolic punctate structures in addition to the cell plates (asterisks in Fig. 1F). It has been reported elsewhere that PM-localized ...
how nuclear pore complexes assemble
how nuclear pore complexes assemble

... within the nuclear envelope, and control molecular exchange between the cytoplasm and nucleoplasm. In this Commentary, we discuss the biogenesis of these huge protein assemblies from approximately one thousand individual proteins. We will summarize current knowledge about distinct assembly modes in ...
The regulation of Krox-20 expression reveals important steps in the
The regulation of Krox-20 expression reveals important steps in the

... Acquisition of Krox-20 inducibility at 15.5 dpc coincides with precursor to Schwann cell transition Since the expression of Krox-20 in the PNS involves axonal signalling, the activation of the gene in vivo at 15.5 dpc raises an important question: is this the consequence of a change in the neurons, ...
SIMULATION OF PROKARYOTIC GENETIC CIRCUITS
SIMULATION OF PROKARYOTIC GENETIC CIRCUITS

... state of each cell as established early in embryonic development (54). Genetic regulatory networks progress asynchronously through successive reactions, so that biological “time” is based more on the degree of progress along reaction pathways than on clock time. The stochastic pattern of signal prot ...
Embodied Computation Applying the Physics of Computation to Artificial Morphogenesis
Embodied Computation Applying the Physics of Computation to Artificial Morphogenesis

... intelligence and cognitive science to suppose that intelligence requires complex internal models of the body and the world in order to behave competently, it has been found that embodied agents can function competently without these complex models by exploiting their bodies and their physical enviro ...
paramecium notes 13 highlighted
paramecium notes 13 highlighted

... Paramecium are unicellular protozoans classified in the Kingdom Protista. Recall that protozoans are animal-like protists, named that way because they move and eat just like animals only they are made of a single cell. Paramecia live in quiet or stagnant ponds and feed on algae scum and other microo ...
CONDENSIN AND COHESIN: MORE THAN CHROMOSOME
CONDENSIN AND COHESIN: MORE THAN CHROMOSOME

... DNA elements, such as enhancers, silencers and insulators, mediate these levels of gene regulation by recruiting trans-acting factors that influence transcription. Recent studies have shown that either cohesin or condensin contributes to each of these levels of regulation, in part by influencing enh ...
Synaptic Regulation of the Light-Dependent Oscillatory Currents in
Synaptic Regulation of the Light-Dependent Oscillatory Currents in

... biocytin shows the characteristic dendritic arborization. The bar in the top right corner is 50 ␮m long. B: representative current-clamp recording from a starburst amacrine cell. Steps of currents were injected in the cell for 600 ms, and the membrane voltage responses were recorded under whole cell ...
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Mitosis



Mitosis is a part of the cell cycle in which chromosomes in a cell nucleus are separated into two identical sets of chromosomes, each in its own nucleus. In general, mitosis (division of the nucleus) is often followed by cytokinesis, which divides the cytoplasm, organelles and cell membrane into two new cells containing roughly equal shares of these cellular components. Mitosis and cytokinesis together define the mitotic (M) phase of an animal cell cycle—the division of the mother cell into two daughter cells, genetically identical to each other and to their parent cell.The process of mitosis is divided into stages corresponding to the completion of one set of activities and the start of the next. These stages are prophase, prometaphase, metaphase, anaphase, and telophase. During mitosis, the chromosomes, which have already duplicated, condense and attach to fibers that pull one copy of each chromosome to opposite sides of the cell. The result is two genetically identical daughter nuclei. The cell may then divide by cytokinesis to produce two daughter cells. Producing three or more daughter cells instead of normal two is a mitotic error called tripolar mitosis or multipolar mitosis (direct cell triplication / multiplication). Other errors during mitosis can induce apoptosis (programmed cell death) or cause mutations. Certain types of cancer can arise from such mutations.Mitosis occurs only in eukaryotic cells and the process varies in different organisms. For example, animals undergo an ""open"" mitosis, where the nuclear envelope breaks down before the chromosomes separate, while fungi undergo a ""closed"" mitosis, where chromosomes divide within an intact cell nucleus. Furthermore, most animal cells undergo a shape change, known as mitotic cell rounding, to adopt a near spherical morphology at the start of mitosis. Prokaryotic cells, which lack a nucleus, divide by a different process called binary fission.
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