Dynamic Proteomics of Individual Cancer Cells in Response to a
... TOP1 also showed rapid localization changes. TOP1 is found in the nucleoli and nucleus of cells before drug addition (24). On CPT treatment, tagged TOP1 intensity in the nucleoli dropped in less than 2 min (Fig. 3A). Fluorescence accumulated in the cytoplasm on the time scale of 5 hours after CPT ad ...
... TOP1 also showed rapid localization changes. TOP1 is found in the nucleoli and nucleus of cells before drug addition (24). On CPT treatment, tagged TOP1 intensity in the nucleoli dropped in less than 2 min (Fig. 3A). Fluorescence accumulated in the cytoplasm on the time scale of 5 hours after CPT ad ...
A Ride with Listeria monocytogenes: A Trojan Horse
... well as storage and protection from extracellular proteases (Dussurget et al., 2004). Another receptor for InlB is some form of the surface associated gC1q-R. However, the specific mechanism by which InlB binds and interacts with gC1q-R remains to be determined. Since this protein lacks a transmembr ...
... well as storage and protection from extracellular proteases (Dussurget et al., 2004). Another receptor for InlB is some form of the surface associated gC1q-R. However, the specific mechanism by which InlB binds and interacts with gC1q-R remains to be determined. Since this protein lacks a transmembr ...
Unit 3 - Madison Public Schools
... Unit 4: Microscopes, Structure and Function of Cells Endomembrane System (EMS) Monkemeier ...
... Unit 4: Microscopes, Structure and Function of Cells Endomembrane System (EMS) Monkemeier ...
General western blot protocol
... cm2 flask; 0.5 mL per 5x106 cells/60 mm dish/75 cm2 flask). 3. Scrape adherent cells off the dish using a cold plastic cell scraper, then gently transfer the cell suspension into a pre-cooled microcentrifuge tube. Alternatively cells can be trypsinized and washed with PBS prior to resuspension in ly ...
... cm2 flask; 0.5 mL per 5x106 cells/60 mm dish/75 cm2 flask). 3. Scrape adherent cells off the dish using a cold plastic cell scraper, then gently transfer the cell suspension into a pre-cooled microcentrifuge tube. Alternatively cells can be trypsinized and washed with PBS prior to resuspension in ly ...
The push and pull of the bacterial cytoskeleton
... components is to physically move them through the cytoplasm to a specific location. In eukaryotic cells, there are motor proteins that directly transport vesicles, mRNA and proteins along tracks of actin or tubulin. However, actin and tubulin themselves can also function as motors to propel objects ...
... components is to physically move them through the cytoplasm to a specific location. In eukaryotic cells, there are motor proteins that directly transport vesicles, mRNA and proteins along tracks of actin or tubulin. However, actin and tubulin themselves can also function as motors to propel objects ...
SPUTUM: PREPARATION AND EXAMINATION OF GRAM STAINED
... brought together again and the process repeated. This may be done several times, if necessary, to obtain a thin, even layer of material on each slide. The finished smears should be spread thinly and evenly over at least half of the available space on the slide. Allow the smears to dry thoroughly bef ...
... brought together again and the process repeated. This may be done several times, if necessary, to obtain a thin, even layer of material on each slide. The finished smears should be spread thinly and evenly over at least half of the available space on the slide. Allow the smears to dry thoroughly bef ...
Photobleaching Substrates Characterized Using Fluorescence
... Similarly, Ig heavy chain class switch recombination, which also contains a DSB intermediate, is similarly inhibited in Ku70-deficient mice (18). Despite the evidence that Ku functions in NHEJ during repair of DSBs, its properties in intact cells are unclear. For example, little is known concerning ...
... Similarly, Ig heavy chain class switch recombination, which also contains a DSB intermediate, is similarly inhibited in Ku70-deficient mice (18). Despite the evidence that Ku functions in NHEJ during repair of DSBs, its properties in intact cells are unclear. For example, little is known concerning ...
to a prolonged period of sucrose deprivation
... of intracellular carbohydrate biosynthesis. Consequently, the supply of organic carbon necessary for sustaining cell respiration may be decreased. However, plant cells, owing to the presence of intracellular pools of carbohydrate and to their ability to control an autophagic process can survive for ...
... of intracellular carbohydrate biosynthesis. Consequently, the supply of organic carbon necessary for sustaining cell respiration may be decreased. However, plant cells, owing to the presence of intracellular pools of carbohydrate and to their ability to control an autophagic process can survive for ...
Rab13 regulates PKA signaling during tight junction assembly
... PKA catalytic subunit. Blotting with the anti-PKA antibody revealed that PKA preferentially bound to GST-Rab13GTPS, but neither to GST alone, nor to GST-Rab6GTPS (Fig. 5 B), indicating that Rab13 interacts directly and specifically with the PKA catalytic subunit. Because the GTP bound form of Rab1 ...
... PKA catalytic subunit. Blotting with the anti-PKA antibody revealed that PKA preferentially bound to GST-Rab13GTPS, but neither to GST alone, nor to GST-Rab6GTPS (Fig. 5 B), indicating that Rab13 interacts directly and specifically with the PKA catalytic subunit. Because the GTP bound form of Rab1 ...
Vascular Plant Morphology Laboratory 1 Internal Anatomy and
... tissues, the vascular cambium and the cork cambium. Vascular cambium produces secondary xylem (inwardly) and secondary phloem (outwardly). Secondary xylem is also called wood. The additional production of secondary xylem and phloem is needed to supply the ever growing portions of the shoot or root. ...
... tissues, the vascular cambium and the cork cambium. Vascular cambium produces secondary xylem (inwardly) and secondary phloem (outwardly). Secondary xylem is also called wood. The additional production of secondary xylem and phloem is needed to supply the ever growing portions of the shoot or root. ...
Editorial What is the true resting potential of small cells?
... solution. Moreover, in both of the above techniques, the membrane potential of cells with high membrane resistance can be influenced by junction potentials between the recording electrode and the cytoplasm or by small offset currents delivered by the recording amplifier or the electrical stimulator. An ...
... solution. Moreover, in both of the above techniques, the membrane potential of cells with high membrane resistance can be influenced by junction potentials between the recording electrode and the cytoplasm or by small offset currents delivered by the recording amplifier or the electrical stimulator. An ...
Physical methods of gene transfer: Kinetics of
... 2000; Lu et al., 2003) was found to be relatively low. Laser irradiation/Photoporation: Lasers were shown to be efficient for introduction of foreign DNA into cultured cells (Kurata et al., 1986). The cells upon laser irradiation undergo a change in the permeability of the plasma membrane or form po ...
... 2000; Lu et al., 2003) was found to be relatively low. Laser irradiation/Photoporation: Lasers were shown to be efficient for introduction of foreign DNA into cultured cells (Kurata et al., 1986). The cells upon laser irradiation undergo a change in the permeability of the plasma membrane or form po ...
Bacteria
... The skeletal remains of large whales are home to a unique genus of gutless polychaete worms called Osedax. These worms harbor heterotrophic bacteria that degrade lipids in whale bones to provide their host with nutrition. ...
... The skeletal remains of large whales are home to a unique genus of gutless polychaete worms called Osedax. These worms harbor heterotrophic bacteria that degrade lipids in whale bones to provide their host with nutrition. ...
Big Idea 4: Biological systems interact, and these systems and their
... d. Mitochondria specialize in energy capture and transformation. [See also 2.A.2, 2.B.3] Evidence of student learning is a demonstrated understanding of each of the following: 1. Mitochondria have a double membrane that allows compartmentalization within the mitochondria and is important to its func ...
... d. Mitochondria specialize in energy capture and transformation. [See also 2.A.2, 2.B.3] Evidence of student learning is a demonstrated understanding of each of the following: 1. Mitochondria have a double membrane that allows compartmentalization within the mitochondria and is important to its func ...
Integr. Comp. Biol., 43:55–63 Epithelium—the primary building block
... been loosely applied to other structures, sometimes even anything composed of more than one cell type or of a single tissue complicatedly folded, this stricter definition, specifying mesenchymal-epithelial interactions, is the only one that conserves the highest information content—anything short of ...
... been loosely applied to other structures, sometimes even anything composed of more than one cell type or of a single tissue complicatedly folded, this stricter definition, specifying mesenchymal-epithelial interactions, is the only one that conserves the highest information content—anything short of ...
Arrest, Adaptation, and Recovery following a Chromosome Double-strand Break in Saccharomyces cerevisiae.
... (Kondo et al. 1999; Green et al. 2000). Another protein, Rad9p, is also required, independent of these other proteins (de la Torre-Ruiz et al. 1998). The ultimate targets of the damage signal cascade include Dun1p (Huang et al. 1998), a protein kinase that controls the induction of many damage-induc ...
... (Kondo et al. 1999; Green et al. 2000). Another protein, Rad9p, is also required, independent of these other proteins (de la Torre-Ruiz et al. 1998). The ultimate targets of the damage signal cascade include Dun1p (Huang et al. 1998), a protein kinase that controls the induction of many damage-induc ...
Differentiation in plant epidermal cells
... differences to produce significantly different cells which may then adopt different fates. This cell interaction mechanism does not rely on cell lineage, and so cells contributing to the development of a single structure may be clonally unrelated. The patterning of Arabidopsis trichomes is controlled t ...
... differences to produce significantly different cells which may then adopt different fates. This cell interaction mechanism does not rely on cell lineage, and so cells contributing to the development of a single structure may be clonally unrelated. The patterning of Arabidopsis trichomes is controlled t ...
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... sections (Figure 1). 3D reconstruction rules these out. We have obtained similar tomograms for nine additional G. obscuriglobus cells with distinct overall membrane organization, and reached the same conclusion in each case (Figures S2, S3, S4, S5, S6, S7, S8). This conclusion is consistent with the ...
... sections (Figure 1). 3D reconstruction rules these out. We have obtained similar tomograms for nine additional G. obscuriglobus cells with distinct overall membrane organization, and reached the same conclusion in each case (Figures S2, S3, S4, S5, S6, S7, S8). This conclusion is consistent with the ...
Death associated proteins (DAPs)
... Keywords: apoptosis; DAP-kinase; cathepsin D; interferon-g; metastasis; functional gene cloning ...
... Keywords: apoptosis; DAP-kinase; cathepsin D; interferon-g; metastasis; functional gene cloning ...
Mitosis
Mitosis is a part of the cell cycle in which chromosomes in a cell nucleus are separated into two identical sets of chromosomes, each in its own nucleus. In general, mitosis (division of the nucleus) is often followed by cytokinesis, which divides the cytoplasm, organelles and cell membrane into two new cells containing roughly equal shares of these cellular components. Mitosis and cytokinesis together define the mitotic (M) phase of an animal cell cycle—the division of the mother cell into two daughter cells, genetically identical to each other and to their parent cell.The process of mitosis is divided into stages corresponding to the completion of one set of activities and the start of the next. These stages are prophase, prometaphase, metaphase, anaphase, and telophase. During mitosis, the chromosomes, which have already duplicated, condense and attach to fibers that pull one copy of each chromosome to opposite sides of the cell. The result is two genetically identical daughter nuclei. The cell may then divide by cytokinesis to produce two daughter cells. Producing three or more daughter cells instead of normal two is a mitotic error called tripolar mitosis or multipolar mitosis (direct cell triplication / multiplication). Other errors during mitosis can induce apoptosis (programmed cell death) or cause mutations. Certain types of cancer can arise from such mutations.Mitosis occurs only in eukaryotic cells and the process varies in different organisms. For example, animals undergo an ""open"" mitosis, where the nuclear envelope breaks down before the chromosomes separate, while fungi undergo a ""closed"" mitosis, where chromosomes divide within an intact cell nucleus. Furthermore, most animal cells undergo a shape change, known as mitotic cell rounding, to adopt a near spherical morphology at the start of mitosis. Prokaryotic cells, which lack a nucleus, divide by a different process called binary fission.