CONNECTIVE TISSUE
... and can be made principally of collagen or elastic fibers. Dense elastic tissue may be found associated with in ligaments, however, dense collagenous is predominant in tendons. a. Tendon (slides CT 8, 9 & 15). The collagen fibers are parallel with long, thin ...
... and can be made principally of collagen or elastic fibers. Dense elastic tissue may be found associated with in ligaments, however, dense collagenous is predominant in tendons. a. Tendon (slides CT 8, 9 & 15). The collagen fibers are parallel with long, thin ...
a-Catulin, a Rho signalling component, can regulate NF
... Using an antibody raised against recombinant a-catulin, we performed immunostaining of HUVEC cells. a-Catulin was distributed throughout the cell, including cytoplasm, cell membrane and nucleus (Figure 3a), with some variations between individual cells. We then generated a set of a-catulin mutants ( ...
... Using an antibody raised against recombinant a-catulin, we performed immunostaining of HUVEC cells. a-Catulin was distributed throughout the cell, including cytoplasm, cell membrane and nucleus (Figure 3a), with some variations between individual cells. We then generated a set of a-catulin mutants ( ...
Homeobox A9 Transcriptionally Regulates the EphB4 Receptor to
... variety of processes including embryonic vascular and neuronal development and may also be involved in adult functions such as synaptic plasticity, proliferation of stem cells, and cell migration.21,22 Recent reports demonstrated a role for the regulation of the EphA2-receptor by homeobox genes duri ...
... variety of processes including embryonic vascular and neuronal development and may also be involved in adult functions such as synaptic plasticity, proliferation of stem cells, and cell migration.21,22 Recent reports demonstrated a role for the regulation of the EphA2-receptor by homeobox genes duri ...
Biol 1020: Membranes
... this model explained the existing data and made two key predications that have been verified: ...
... this model explained the existing data and made two key predications that have been verified: ...
mRNA ISH Yeast
... *Please note: For optimal fixation it may be critical to use fresh formaldehyde solutions. Fresh 4% solutions can be made from 16%, methanol free, formaldehyde or from solid paraformaldehyde (4% w/v). For preparation of buffers please refer to : Molecular cloning : a laboratory manual / Sambrook, Jo ...
... *Please note: For optimal fixation it may be critical to use fresh formaldehyde solutions. Fresh 4% solutions can be made from 16%, methanol free, formaldehyde or from solid paraformaldehyde (4% w/v). For preparation of buffers please refer to : Molecular cloning : a laboratory manual / Sambrook, Jo ...
Stefanie Dimmeler Thomas Bruhl, Carmen Urbich
... variety of processes including embryonic vascular and neuronal development and may also be involved in adult functions such as synaptic plasticity, proliferation of stem cells, and cell migration.21,22 Recent reports demonstrated a role for the regulation of the EphA2-receptor by homeobox genes duri ...
... variety of processes including embryonic vascular and neuronal development and may also be involved in adult functions such as synaptic plasticity, proliferation of stem cells, and cell migration.21,22 Recent reports demonstrated a role for the regulation of the EphA2-receptor by homeobox genes duri ...
CycD1, a Putative G1 Cyclin from Antirrhinum majus
... However, constitutive expression of positive cell cycle regulators can be difficult to interpret because an effect in one phase of the cycle may be masked by compensatory changes in another. Cooper (1998) argued that it is not possible to distinguish the phase-specific effects of G1 cyclin expressio ...
... However, constitutive expression of positive cell cycle regulators can be difficult to interpret because an effect in one phase of the cycle may be masked by compensatory changes in another. Cooper (1998) argued that it is not possible to distinguish the phase-specific effects of G1 cyclin expressio ...
Cell cycle analysis
... Supplementary Figure S6 The DNA damage sensitivity of hht1 K56R mutant cells cannot be accounted for simply by defects in Non-Homologous DNA End-Joining (NHEJ). a, 10-fold serial dilutions of wild-type (HMY 57), hht1 K56R (HMY 160) and yku70 (HMY 146) mutant strains were analysed for colony formati ...
... Supplementary Figure S6 The DNA damage sensitivity of hht1 K56R mutant cells cannot be accounted for simply by defects in Non-Homologous DNA End-Joining (NHEJ). a, 10-fold serial dilutions of wild-type (HMY 57), hht1 K56R (HMY 160) and yku70 (HMY 146) mutant strains were analysed for colony formati ...
Cell culture and transfection
... (http://www.affymetrix.com/products/arrays/specific/rgu34.affx) for 16h and rotated at 13 rpm at 50oC. Bacterial spike controls were added to the hybridization mixture. Arrays were then washed and stained with streptavidin-phycoerythrin (Molecular Probes, Eugene, OR), a goat IgG antibody, and then a ...
... (http://www.affymetrix.com/products/arrays/specific/rgu34.affx) for 16h and rotated at 13 rpm at 50oC. Bacterial spike controls were added to the hybridization mixture. Arrays were then washed and stained with streptavidin-phycoerythrin (Molecular Probes, Eugene, OR), a goat IgG antibody, and then a ...
Plant Bioreactor Design
... However, the use of high gassing rates to achieve good mixing can have problems: Plant cell suspensions sensitive to the level of carbon dioxide. Other essential volatiles such as ethylene can be stripped off with high aeration rates. ...
... However, the use of high gassing rates to achieve good mixing can have problems: Plant cell suspensions sensitive to the level of carbon dioxide. Other essential volatiles such as ethylene can be stripped off with high aeration rates. ...
Lab Test 1 Study Sheet (summer term)
... onion 23. Recognize some of the major structures observable in the cells, e.g., nucleus, cell wall, cell membrane, chloroplast. 24. Be able to recognize the protists observed. 25. Know how to use the t-distribution table to determine the t – critical value. 26. Be able to use the t-critical value an ...
... onion 23. Recognize some of the major structures observable in the cells, e.g., nucleus, cell wall, cell membrane, chloroplast. 24. Be able to recognize the protists observed. 25. Know how to use the t-distribution table to determine the t – critical value. 26. Be able to use the t-critical value an ...
CycD1, a Putative G1 Cyclin from Antirrhinum majus, Accelerates
... However, constitutive expression of positive cell cycle regulators can be difficult to interpret because an effect in one phase of the cycle may be masked by compensatory changes in another. Cooper (1998) argued that it is not possible to distinguish the phase-specific effects of G1 cyclin expressio ...
... However, constitutive expression of positive cell cycle regulators can be difficult to interpret because an effect in one phase of the cycle may be masked by compensatory changes in another. Cooper (1998) argued that it is not possible to distinguish the phase-specific effects of G1 cyclin expressio ...
End4/Sla2 is involved in establishment of a new growth zone in
... to F-actin (McCann and Craig, 1999). Cells bearing this point with extreme polarity defects had microtubules curling under mutation (Sla2R1083G) in End4/Sla2 showed NETO defects; the cortex (data not shown). However, at 25°C cells that still 62% of cells grew in a monopolar fashion with a monopolar ...
... to F-actin (McCann and Craig, 1999). Cells bearing this point with extreme polarity defects had microtubules curling under mutation (Sla2R1083G) in End4/Sla2 showed NETO defects; the cortex (data not shown). However, at 25°C cells that still 62% of cells grew in a monopolar fashion with a monopolar ...
Use of fluorescently labeled Alt-R™ CRISPR
... In performing FACS analysis, we observed that flow cytometric resolution was time dependent. As Figure 4 shows, this was evident when using 2 RNP concentrations (0.15 µM and 1.5 µM) in 2 different cell types (Jurkat and HEK-293). At both RNP concentrations, fluorescence intensity decreased with time ...
... In performing FACS analysis, we observed that flow cytometric resolution was time dependent. As Figure 4 shows, this was evident when using 2 RNP concentrations (0.15 µM and 1.5 µM) in 2 different cell types (Jurkat and HEK-293). At both RNP concentrations, fluorescence intensity decreased with time ...
Bacterial dormancy and culturability: the role of
... manifest culturability when incubated under different circumstances. The usual convention for describing cells that have reversibly lost the ability to proliferate is to refer to them as being ‘dormant’ or ‘anabiotic’. Thus, and given that the phenomenon of dormancy necessarily admits the concept of ...
... manifest culturability when incubated under different circumstances. The usual convention for describing cells that have reversibly lost the ability to proliferate is to refer to them as being ‘dormant’ or ‘anabiotic’. Thus, and given that the phenomenon of dormancy necessarily admits the concept of ...
Forage Values of Range Plants
... The portion of shrubs used for forage. Generally, leaves & current seasons twigs. ...
... The portion of shrubs used for forage. Generally, leaves & current seasons twigs. ...
Gastrulation in C. elegans
... 2. Polarization of gastrulating cells The cell movements of gastrulation begin at the 26-cell stage when the two endodermal precursors, Ea and Ep, move from the surface of the embryo into a small interior cavity called the blastocoel (Sulston et al., 1983). Prior to the 26-cell stage, the embryo is ...
... 2. Polarization of gastrulating cells The cell movements of gastrulation begin at the 26-cell stage when the two endodermal precursors, Ea and Ep, move from the surface of the embryo into a small interior cavity called the blastocoel (Sulston et al., 1983). Prior to the 26-cell stage, the embryo is ...
Document
... II. Budding • Division of one parent individual into two or more unequal daughter individuals. • The smaller daughter individual must mature. ...
... II. Budding • Division of one parent individual into two or more unequal daughter individuals. • The smaller daughter individual must mature. ...
Mitosis
Mitosis is a part of the cell cycle in which chromosomes in a cell nucleus are separated into two identical sets of chromosomes, each in its own nucleus. In general, mitosis (division of the nucleus) is often followed by cytokinesis, which divides the cytoplasm, organelles and cell membrane into two new cells containing roughly equal shares of these cellular components. Mitosis and cytokinesis together define the mitotic (M) phase of an animal cell cycle—the division of the mother cell into two daughter cells, genetically identical to each other and to their parent cell.The process of mitosis is divided into stages corresponding to the completion of one set of activities and the start of the next. These stages are prophase, prometaphase, metaphase, anaphase, and telophase. During mitosis, the chromosomes, which have already duplicated, condense and attach to fibers that pull one copy of each chromosome to opposite sides of the cell. The result is two genetically identical daughter nuclei. The cell may then divide by cytokinesis to produce two daughter cells. Producing three or more daughter cells instead of normal two is a mitotic error called tripolar mitosis or multipolar mitosis (direct cell triplication / multiplication). Other errors during mitosis can induce apoptosis (programmed cell death) or cause mutations. Certain types of cancer can arise from such mutations.Mitosis occurs only in eukaryotic cells and the process varies in different organisms. For example, animals undergo an ""open"" mitosis, where the nuclear envelope breaks down before the chromosomes separate, while fungi undergo a ""closed"" mitosis, where chromosomes divide within an intact cell nucleus. Furthermore, most animal cells undergo a shape change, known as mitotic cell rounding, to adopt a near spherical morphology at the start of mitosis. Prokaryotic cells, which lack a nucleus, divide by a different process called binary fission.