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Journal of Cell Science • Advance article Rop, the
Journal of Cell Science • Advance article Rop, the

... some hypomorphic Rop mutants survive into larval stages (Table 1). At the permissive and restrictive temperatures, 27.0% and 0.9% of embryos from RopA19/G11 parents hatched (Table S3 in supplementary material). Normal hatch rates for wild type embryos of 87.6% at 25°C and 86.3% at 32°C showed that t ...
Maturation-promoting Factor Induces Nuclear Envelope Breakdown
Maturation-promoting Factor Induces Nuclear Envelope Breakdown

... and G2 phases (14), so the cycle consists of only two phases: M (mitosis) and S (DNA synthesis). There is no transcription during this period (28). In short, the primary function of this stage of development seems to be the rapid and orderly replication of DNA and the subdivision of the cytoplasm to ...
Mechanistic investigation into the actions of taurine on beta cells
Mechanistic investigation into the actions of taurine on beta cells

... Taurine (2-aminoethanesulphonic acid) is a semi-essential amino acid, which is not incorporated into proteins. In mammalian tissues, taurine is ubiquitous and is the most abundant free amino acid in the heart, retina, skeletal muscle, brain, and leukocytes, exhibiting multiple functions and tissue-p ...
Coupling cellular oscillators—circadian and cell division cycles in cyanobacteria Bernardo F Pando
Coupling cellular oscillators—circadian and cell division cycles in cyanobacteria Bernardo F Pando

... but also that it regulates cell division by ‘circadian gating’. After synchronizing a population of cells with 12 h cycles of light and dark, the population was grown in an environment of constant light. Surprisingly it was observed that the rate of cell divisions is modulated with a 24 h period as ...
Receptors and immune sensors: the complex entry path of human
Receptors and immune sensors: the complex entry path of human

... consequences of its activation by HCMV. This virus is known to induce cell functions associated with progression through the cell cycle and to co-opt several pathways in an ostensible need to optimize the environment for viral replication events. Wang et al. reported that EGFR was cleared from the c ...
PDF
PDF

... embryos with mRNA in which the 2-OST coding region is fused in-frame to GFP, generating a larger 2-OST protein that could be detected as a discrete band at 69 kDa by western blot analysis. The 2-OST protein levels were normalized to the levels of tubulin for each sample, and then the total levels of ...
Biology 13100 (by Ken Robinson, revised 2009 by NPelaez) The
Biology 13100 (by Ken Robinson, revised 2009 by NPelaez) The

... In the fully open state, the guard cells of a stoma have a high osmotic pressure that is produced by high concentrations of K+ and Cl-, which results in water influx and high turgor pressure. As the elongated guard cells are attached to each other at their ends, the turgor pressure causes the cells ...
Is the Cytoskeleton Necessary for Viral Replication?
Is the Cytoskeleton Necessary for Viral Replication?

... pulling the centrosomes to the poles of the cell. Cell division occurs after mitosis is complete (Pollard et al., 2008). Cancer researchers often use mitosis-disrupting drugs to study the role of abnormal cell division in cancer development. In normal cells, factors that interfere with microtubule f ...
Downloaded - The Journal of Immunology
Downloaded - The Journal of Immunology

... cell-mediated immunity, we have used dendritic cells (DCs) generated by a 3-day culture of human CD14ⴙ monocytes in the presence of GM-CSF and type I IFN (IFN-DCs) and pulsed with peptides corresponding to CTL EBV epitopes. The functional activity of IFN-DCs was compared with that of APCs differenti ...
Transient pluripotent cell populations in vivo
Transient pluripotent cell populations in vivo

... delineating this time in embryogenesis have been described. Of the reported proteins, the POU domain transcription factor Oct4 can be used to recognize all pluripotent cell populations (Rosner et al., 1990; Scholer et al., 1990), but only the Fgf5 (Haub and Goldfarb, 1991; Hebert et al., 1991), Rex1 ...
Bacterial ancestry of actin and tubulin Fusinita van den Ent, Linda
Bacterial ancestry of actin and tubulin Fusinita van den Ent, Linda

... obvious candidate for an MSP-like protein in bacteria. Dynamic polymerisation is only one mechanism by which actin and tubulin achieve some of their specific functions. The polar nature of microtubules and actin filaments controls the direction of motor proteins and hence enables the spatial organis ...
Chapter 9 - Fullfrontalanatomy.com
Chapter 9 - Fullfrontalanatomy.com

... not be able to express your wishes to others, nor could you ask questions to find out more about your environment. Social organization is dependent on communication between the individuals that comprise that society; without communication, society would fall apart. As with people, it is vital for in ...
Reference
Reference

... years, which are essential for the success of the project. Deployment of larger instruments such as mass spectrometers, confocal microscope, fluorescence and chemiluminescence analyzers, together with biochips allow the researchers involved in this project to compete internationally and pursuit the ...
Importance of T-Cell Receptor -Chain Gene Analysis
Importance of T-Cell Receptor -Chain Gene Analysis

... expression of cyCD3e.3 In addition, we reported 6 patients with CD71 early T-ALL/lymphoblastic lymphoma (LBL) which were thought to be transformed from a pro-T I or II cell, indicating T-stem cell leukemia/ lymphoma. They had rearrangement in DDJ(5/6) or VDJ(1/6) of TCR d locus.8 All patients achiev ...
A Novel Temporal Expression Pattern of Three C
A Novel Temporal Expression Pattern of Three C

... in these extracts was again monitored by Ponceau S staining (Fig lC, bottom panel). Total cellular protein diminished approximately twofold during granulocytic differentiation. Given the modest decrease in total cellular protein in this experiment, and given the large decrease in nuclear volume duri ...
Solubilization and Analysis of Mannoprotein Molecules from The
Solubilization and Analysis of Mannoprotein Molecules from The

Microinjection into plant cells of etiolated - Products
Microinjection into plant cells of etiolated - Products

... after germination (Sinapis alba 4 dD, Lycopersicon esculentum 6 dD, Arabidopsis thaliana 4 dD). Germination of Arabidopsis seedlings was induced by 2 days of cold treatment (4°C) and subsequent irradiation with white light for 6 h. Prior to microinjection the seedlings were placed into a lid of a pe ...
Meristem-Specific Suppression of Mitosis and a
Meristem-Specific Suppression of Mitosis and a

... specific cell types their unique properties, the regulatory genes and receptors that control the process, or the signals that trigger it (Jacobs, 1994). Studies in our laboratory have confirmed an observation first documented in 1919: the sloughed root cap cells, which separate in large numbers from ...
Genomic Tagging of the Anaphase-Promoting Complex Activator
Genomic Tagging of the Anaphase-Promoting Complex Activator

... propensity for drastically deregulating the cell cycle. Findings of Liu et al. (10) indicate that Tax directly interacts with the APC/CCdc20, causing a number of cellular events that in the long run commit the cells to senescence and cause growth arrest. Tax binds and activates APC/C ahead of its re ...
Cell cycle–dependent phosphorylation of Sec4p controls membrane
Cell cycle–dependent phosphorylation of Sec4p controls membrane

... spatially and temporally localizes with Cdc5p exclusively when Sec4p phosphorylation levels peak during the cell cycle, indicating Sec4p is a direct Cdc5p substrate. Our data suggest the physiological relevance of Sec4p phosphorylation is to facilitate the coordination of membrane-trafficking events ...
New type of snRNP containing nuclear bodies in plant cells
New type of snRNP containing nuclear bodies in plant cells

... movement within the cell nucleus demonstrated that CB and NAB present different locations of the same structure. This work presents a different, hitherto unknown type of nuclear bodies containing elements of the splicing system in a plant cell. These bodies were described for the first time in larch ...
to the complete text
to the complete text

... the integrity of the actin cytoskeleton. Inactivation of the racE gene in Dictyostelium, for example, results in abnormal actin aggregates and defects in cytokinesis suggesting a role for racE in the organization of the actin cytoskeleton [14]. Overexpression of RacC, another Rac isoform, has been s ...
Quantification of gap junction selectivity
Quantification of gap junction selectivity

... To understand why the mammalian genome includes 20 or more connexin genes, the functional differences between the gap junctions that they form must be appreciated. Since their discovery, much has been learned about gap junctions in a broad sense, but their functional and regulatory differences are s ...
Topics Standard cell layout Standard cell structure Standard cell
Topics Standard cell layout Standard cell structure Standard cell

... Spacing between tracks is center-to-center distance between wires. Track spacing depends on wire layer used. ...
spatial control of cell expansion by the plant cytoskeleton
spatial control of cell expansion by the plant cytoskeleton

... by University of California - San Diego on 10/18/05. For personal use only. ...
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Mitosis



Mitosis is a part of the cell cycle in which chromosomes in a cell nucleus are separated into two identical sets of chromosomes, each in its own nucleus. In general, mitosis (division of the nucleus) is often followed by cytokinesis, which divides the cytoplasm, organelles and cell membrane into two new cells containing roughly equal shares of these cellular components. Mitosis and cytokinesis together define the mitotic (M) phase of an animal cell cycle—the division of the mother cell into two daughter cells, genetically identical to each other and to their parent cell.The process of mitosis is divided into stages corresponding to the completion of one set of activities and the start of the next. These stages are prophase, prometaphase, metaphase, anaphase, and telophase. During mitosis, the chromosomes, which have already duplicated, condense and attach to fibers that pull one copy of each chromosome to opposite sides of the cell. The result is two genetically identical daughter nuclei. The cell may then divide by cytokinesis to produce two daughter cells. Producing three or more daughter cells instead of normal two is a mitotic error called tripolar mitosis or multipolar mitosis (direct cell triplication / multiplication). Other errors during mitosis can induce apoptosis (programmed cell death) or cause mutations. Certain types of cancer can arise from such mutations.Mitosis occurs only in eukaryotic cells and the process varies in different organisms. For example, animals undergo an ""open"" mitosis, where the nuclear envelope breaks down before the chromosomes separate, while fungi undergo a ""closed"" mitosis, where chromosomes divide within an intact cell nucleus. Furthermore, most animal cells undergo a shape change, known as mitotic cell rounding, to adopt a near spherical morphology at the start of mitosis. Prokaryotic cells, which lack a nucleus, divide by a different process called binary fission.
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