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somatic hybridization - GCG-42
somatic hybridization - GCG-42

... Regeneration of hybrid plants • Plants are induced to regenerate from hybrid calli. • These hybrid plants must be at least partially fertile, in addition to having some useful property, to be of any use in breeding schemes. ...
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... liphophylic dye, merocyanine 540 (MC540), which selectively stains disordered or loosely packed membranes (Williamson et al, 1983; McEvoy et al, 1988). Irradiated HL-60 were stained with MC540 to detect changes in lipid distribution at various times thereafter. As shown in Figure 2B, before irradiat ...
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... The protein has an N terminal signal that is required for translocation. The N terminal signal does not appear to be cleaved during translocation. The protein will translocate through the endosomal channel even in the absence of the bacterial membrane channel. Most students had correct answers for t ...
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... replication and repair are well known, our understanding of the mechanisms of chromosomal DNA partitioning is largely incomplete. While the relatively small size of the bacterial cell has hampered efforts to visualize specific structures within the cell that might constitute a mitotic apparatus, the ...
BIOLOGY I MIDTERM OBJECTIVES Chapter 1 (The Nature of
BIOLOGY I MIDTERM OBJECTIVES Chapter 1 (The Nature of

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Mitosis



Mitosis is a part of the cell cycle in which chromosomes in a cell nucleus are separated into two identical sets of chromosomes, each in its own nucleus. In general, mitosis (division of the nucleus) is often followed by cytokinesis, which divides the cytoplasm, organelles and cell membrane into two new cells containing roughly equal shares of these cellular components. Mitosis and cytokinesis together define the mitotic (M) phase of an animal cell cycle—the division of the mother cell into two daughter cells, genetically identical to each other and to their parent cell.The process of mitosis is divided into stages corresponding to the completion of one set of activities and the start of the next. These stages are prophase, prometaphase, metaphase, anaphase, and telophase. During mitosis, the chromosomes, which have already duplicated, condense and attach to fibers that pull one copy of each chromosome to opposite sides of the cell. The result is two genetically identical daughter nuclei. The cell may then divide by cytokinesis to produce two daughter cells. Producing three or more daughter cells instead of normal two is a mitotic error called tripolar mitosis or multipolar mitosis (direct cell triplication / multiplication). Other errors during mitosis can induce apoptosis (programmed cell death) or cause mutations. Certain types of cancer can arise from such mutations.Mitosis occurs only in eukaryotic cells and the process varies in different organisms. For example, animals undergo an ""open"" mitosis, where the nuclear envelope breaks down before the chromosomes separate, while fungi undergo a ""closed"" mitosis, where chromosomes divide within an intact cell nucleus. Furthermore, most animal cells undergo a shape change, known as mitotic cell rounding, to adopt a near spherical morphology at the start of mitosis. Prokaryotic cells, which lack a nucleus, divide by a different process called binary fission.
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