Electrochromatography
... biopolymers - use a gel stationary phase (usually polyacrylamide, dextran, or agarose) at low pressures synthetic polymers - use either a silica or crosslinked polystyrene stationary phase at higher pressures Various mobile phases can be used ...
... biopolymers - use a gel stationary phase (usually polyacrylamide, dextran, or agarose) at low pressures synthetic polymers - use either a silica or crosslinked polystyrene stationary phase at higher pressures Various mobile phases can be used ...
Techniques
... 3. ___________- Detect RNA of ____ of expressed genes 4. ________ ( Reverse transcription polymerase chain reaction- to detect RNA) 5. ________________________ Detect protein 6. _______________- Detect proteins in situ 7. _________- protein-DNA interactions 8. ________– Protein-protein interactions ...
... 3. ___________- Detect RNA of ____ of expressed genes 4. ________ ( Reverse transcription polymerase chain reaction- to detect RNA) 5. ________________________ Detect protein 6. _______________- Detect proteins in situ 7. _________- protein-DNA interactions 8. ________– Protein-protein interactions ...
TOPIC 4.4 Genetic Engineering Worksheet
... 2. Explain how the universality of the genetic code is central to gene transfer ...
... 2. Explain how the universality of the genetic code is central to gene transfer ...
Rapid screening of PCR products using a novel agarose gel
... Ready-To-Run™ Electrophoresis System is designed for the rapid screening of large numbers of PCR products. The modular system features precast gel cassettes; a separation unit with built-in power supply, timer and electrodes; sample loading guide plate; and satellite separation units, each with buil ...
... Ready-To-Run™ Electrophoresis System is designed for the rapid screening of large numbers of PCR products. The modular system features precast gel cassettes; a separation unit with built-in power supply, timer and electrodes; sample loading guide plate; and satellite separation units, each with buil ...
QIAquick Gel Extraction Kit Protocol
... Nucleic acids purification 9) (Optional): Add 500 µL of Buffer QG to QIAquick column and centrifuge for 1 minute. This step will remove all traces of agarose. It is only required when the DNA will subsequently be used for direct sequencing, in vitro transcription or microinjection. 10) To wash, add ...
... Nucleic acids purification 9) (Optional): Add 500 µL of Buffer QG to QIAquick column and centrifuge for 1 minute. This step will remove all traces of agarose. It is only required when the DNA will subsequently be used for direct sequencing, in vitro transcription or microinjection. 10) To wash, add ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.