C8. Nucleic Acids
... (increased is quantity via PCR). The DNA in each blood sample is then cut using restriction enzymes and the DNA fragments are placed in separate chambers of a sheet of gel in an electrophoresis chamber. After an electrical current has been run through the gel, you, the famous paternity judge, view t ...
... (increased is quantity via PCR). The DNA in each blood sample is then cut using restriction enzymes and the DNA fragments are placed in separate chambers of a sheet of gel in an electrophoresis chamber. After an electrical current has been run through the gel, you, the famous paternity judge, view t ...
Accurate identification of plants
... The seemingly arcane field of identifying tree roots has in fact an important practical application. In assessing building damage caused by subsidence due to tree roots, it is important for insurance and compensation purposes to be able to identify the offending trees from analysis of the roots. An ...
... The seemingly arcane field of identifying tree roots has in fact an important practical application. In assessing building damage caused by subsidence due to tree roots, it is important for insurance and compensation purposes to be able to identify the offending trees from analysis of the roots. An ...
2007b
... 1. Describe the properties of the general transcription factors of RNA polymerase II, I and III. What is TBP and what general transcription factors have TBP as a component? What enzymatic activities do some of the transcription factors possess and how do they facilitate transcription? Describe TAFs ...
... 1. Describe the properties of the general transcription factors of RNA polymerase II, I and III. What is TBP and what general transcription factors have TBP as a component? What enzymatic activities do some of the transcription factors possess and how do they facilitate transcription? Describe TAFs ...
Biotechnology
... • Are GMO’s safe for people and the environment? • Does embryonic stem cell research kill babies or simply use human tissue for the good of mankind? • Should people be allowed to choose the trait of their child? ...
... • Are GMO’s safe for people and the environment? • Does embryonic stem cell research kill babies or simply use human tissue for the good of mankind? • Should people be allowed to choose the trait of their child? ...
Introduction to Gel Electrophorsis
... Agarose Gel Electrophoresis • The voltage applied to the gel affects how quickly the gel runs • The higher the voltage, the more quickly the gel runs………But that often reduces the quality of the DNA separation • >>>>>>>>>>It also generates heat which reduces the quality of the DNA separation ...
... Agarose Gel Electrophoresis • The voltage applied to the gel affects how quickly the gel runs • The higher the voltage, the more quickly the gel runs………But that often reduces the quality of the DNA separation • >>>>>>>>>>It also generates heat which reduces the quality of the DNA separation ...
Unit 4: Genetics
... • 1) Future understanding of many genetic diseases. • 2) Advanced, targeted pharmaceutical production. • 3) Bioethical implications, e.g. potential genetic discrimination. Courtesy of David Richfield ...
... • 1) Future understanding of many genetic diseases. • 2) Advanced, targeted pharmaceutical production. • 3) Bioethical implications, e.g. potential genetic discrimination. Courtesy of David Richfield ...
1 PROTOCOLS FOR LIGATION-INDEPENDENT CLONING
... 1. Amplify the insert and vector by PCR using a minimum amount of vector plasmid template (0.5 ng template/50 µL reaction) according to manufacturer’s instructions with a high fidelity DNA polymerase, such as Phusion Hot Start II High Fidelity DNA Polymerase, but omit the final extension step. An en ...
... 1. Amplify the insert and vector by PCR using a minimum amount of vector plasmid template (0.5 ng template/50 µL reaction) according to manufacturer’s instructions with a high fidelity DNA polymerase, such as Phusion Hot Start II High Fidelity DNA Polymerase, but omit the final extension step. An en ...
Restriction Enzymes - Seattle Central College
... • The DNA digested by the restriction enzymes used in the experiment is Lambda DNA, which comes from bacterial virus, or bacteriophage. • Bacteriophage attacks bacteria by inserting its nucleic acid into the host bacteria which replicates rapidly inside host cells until cells burst. The released pha ...
... • The DNA digested by the restriction enzymes used in the experiment is Lambda DNA, which comes from bacterial virus, or bacteriophage. • Bacteriophage attacks bacteria by inserting its nucleic acid into the host bacteria which replicates rapidly inside host cells until cells burst. The released pha ...
Principles of Nucleic Acid Separation by Agarose Gel Electrophoresis
... Fig. 2. Chemical formula of ethidium bromide. An alternative dsDNA stain is SYBR Green I, produced by Invitrogen. Despite the fact that SYBR Green is more expensive, it is 25 times more sensitive than ethidium bromide (Jin et al., 1994). SYBR Safe, a variant of SYBR Green, has been shown to have low ...
... Fig. 2. Chemical formula of ethidium bromide. An alternative dsDNA stain is SYBR Green I, produced by Invitrogen. Despite the fact that SYBR Green is more expensive, it is 25 times more sensitive than ethidium bromide (Jin et al., 1994). SYBR Safe, a variant of SYBR Green, has been shown to have low ...
Miscellaneous Bioseparation
... A common gel material for the study of proteins is cross-linked polyacrylamide In most cases, the goal of experiment is to separate a sample according to molar masses of its components However, the shape and charge will also determine the drift speed One way to avoid this problem and to effect separ ...
... A common gel material for the study of proteins is cross-linked polyacrylamide In most cases, the goal of experiment is to separate a sample according to molar masses of its components However, the shape and charge will also determine the drift speed One way to avoid this problem and to effect separ ...
Acrylamide -gel patterns of total soluble proteins at different stages +
... tube containing 250 .Ll of the homogenization medium. The wall of the tube was washed with the same medium, up to a final volume of 0.5 ml. The tube was then centrifuged at 10,000 x g for 20’ and the supernatant was considered as a dilution of the original hemolymph. Acrylamide-gel electrophoresis w ...
... tube containing 250 .Ll of the homogenization medium. The wall of the tube was washed with the same medium, up to a final volume of 0.5 ml. The tube was then centrifuged at 10,000 x g for 20’ and the supernatant was considered as a dilution of the original hemolymph. Acrylamide-gel electrophoresis w ...
Electrophoretic Theory Appendix A: Electrophoretic Theory
... When voltage is set constant, current and wattage will decrease as the resistance increases, resulting in a decrease of heat and DNA migration. Since the heat generated will decrease, the margin of safety will increase over the length of the run. If a problem develops and the resistance increases dr ...
... When voltage is set constant, current and wattage will decrease as the resistance increases, resulting in a decrease of heat and DNA migration. Since the heat generated will decrease, the margin of safety will increase over the length of the run. If a problem develops and the resistance increases dr ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.