Biotechnology Notes
... DNA Fingerprinting • Made by restrictive enzymes and gel electrophoresis (mother) (child 1) (child 2) (father) • What is it used for? ...
... DNA Fingerprinting • Made by restrictive enzymes and gel electrophoresis (mother) (child 1) (child 2) (father) • What is it used for? ...
20-DNA-technology
... created by Edwin Southern (1975) for locating gene specific sequences on DNA fragments. SOUTHERN BLOTTING: is used to locate and identify genes on DNA. DNA restriction fragments are electrophoretically separated. The fragments are blotted onto membranes, where the DNA bonds. Hybridization with label ...
... created by Edwin Southern (1975) for locating gene specific sequences on DNA fragments. SOUTHERN BLOTTING: is used to locate and identify genes on DNA. DNA restriction fragments are electrophoretically separated. The fragments are blotted onto membranes, where the DNA bonds. Hybridization with label ...
Basic Biochemistry - Personal Webspace for QMUL
... pH when the net surface charge is ZERO for that protein Isoelectric Focusing This technique requires a pH gradient gel It uses a gel of polyampholytes Polyampholytes are small multi-charged polymers with different values of pI Applying an electric field to this gel creates the pH gradient ...
... pH when the net surface charge is ZERO for that protein Isoelectric Focusing This technique requires a pH gradient gel It uses a gel of polyampholytes Polyampholytes are small multi-charged polymers with different values of pI Applying an electric field to this gel creates the pH gradient ...
I have.. Who has.. DNA produced from mRNA by reverse
... of repeated sequences, the number of repeats varying from one individual to another minisatellite. ...
... of repeated sequences, the number of repeats varying from one individual to another minisatellite. ...
The Importance of NGS Sample Prep The manipulation of DNA
... “Bioanalyzer results suggested that [Pippin] automated size-selection libraries were substantially more consistent than gel extraction libraries. In contrast to automated size-selected samples, gel excision samples did not appear to saturate in th range of coverage observed. This is likely because s ...
... “Bioanalyzer results suggested that [Pippin] automated size-selection libraries were substantially more consistent than gel extraction libraries. In contrast to automated size-selected samples, gel excision samples did not appear to saturate in th range of coverage observed. This is likely because s ...
Proteomics pathway Most common properties of proteins
... Electrophoretical separations Gel pI pI/Mr Mr ...
... Electrophoretical separations Gel pI pI/Mr Mr ...
Frontiers of Genetics
... species, into a single DNA molecule • Bacteria have small circular pieces of DNA called plasmids separate from their larger single chromosome • Plasmids can replicate and pass between bacterial cells allowing gene sharing – associated with antibacterial resistance ...
... species, into a single DNA molecule • Bacteria have small circular pieces of DNA called plasmids separate from their larger single chromosome • Plasmids can replicate and pass between bacterial cells allowing gene sharing – associated with antibacterial resistance ...
HS SDS-PAGE (msword 21,1 kB)
... front of the fume hood. In case of a long power or ventilation failure the lab must be evacuated. 4. Run the gel (electrophoresis) Ensure all equipment is in good working order, and that lid is always on electrophoresis tank when in operation. It is important to note that the voltage and currents us ...
... front of the fume hood. In case of a long power or ventilation failure the lab must be evacuated. 4. Run the gel (electrophoresis) Ensure all equipment is in good working order, and that lid is always on electrophoresis tank when in operation. It is important to note that the voltage and currents us ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.