PROTEIN SYNTHESIS
... (i) aminoacyl (A) site: contains IF2-GTP but will contain the incoming tRNA. (ii) peptidyl (P) site: contains tRNAfmet but will contain the growing nascent chain. Specific segments of 16S & 23S rRNAs have been identified that correspond to the A and P sites. Many antibiotics act by binding or blocki ...
... (i) aminoacyl (A) site: contains IF2-GTP but will contain the incoming tRNA. (ii) peptidyl (P) site: contains tRNAfmet but will contain the growing nascent chain. Specific segments of 16S & 23S rRNAs have been identified that correspond to the A and P sites. Many antibiotics act by binding or blocki ...
Lecture 25
... the presence of BP Clonase enzyme master mix. The reaction is incubated for just an hour at 25°C to obtain the entry or master clones containing the gene of interest. Once this master clone, flanked by attL sites is produced, it can then be transferred into any destination vector to produce expressi ...
... the presence of BP Clonase enzyme master mix. The reaction is incubated for just an hour at 25°C to obtain the entry or master clones containing the gene of interest. Once this master clone, flanked by attL sites is produced, it can then be transferred into any destination vector to produce expressi ...
8. ARTÍCULOS
... The human HERC protein family consists of four proteins sharing a HECT domain in their carboxyl-termini and one or more RCC1-like domains (RLDs) elsewhere in their amino acid sequences. The HECT domain (homologous to E6-AP carboxyl-terminus) was ¢rst characterized as the domain involved in the E6-AP ...
... The human HERC protein family consists of four proteins sharing a HECT domain in their carboxyl-termini and one or more RCC1-like domains (RLDs) elsewhere in their amino acid sequences. The HECT domain (homologous to E6-AP carboxyl-terminus) was ¢rst characterized as the domain involved in the E6-AP ...
Polyubiquitination in diseases: implications in skeletal muscle
... Polyubiquitination of lysine initiates proteasome-dependent degradation of target proteins, a process involved in both the pathogenic mechanisms of various diseases and in normal biological function. Recently we investigated polyubiquitination involves skeletal muscle atrophy by degradation of MyoD ...
... Polyubiquitination of lysine initiates proteasome-dependent degradation of target proteins, a process involved in both the pathogenic mechanisms of various diseases and in normal biological function. Recently we investigated polyubiquitination involves skeletal muscle atrophy by degradation of MyoD ...
J-Domain Protein CDJ2 and HSP70B Are a Plastidic Chaperone
... Chaperones of the heat shock protein (Hsp)70 family belong to the most conserved proteins known. Except for some Archaea, Hsp70s are found in all known organisms and are present in every compartment of the eukaryotic cell (Bukau and Horwich, 1998). Principally, Hsp70s consist of an Nterminal ATPase ...
... Chaperones of the heat shock protein (Hsp)70 family belong to the most conserved proteins known. Except for some Archaea, Hsp70s are found in all known organisms and are present in every compartment of the eukaryotic cell (Bukau and Horwich, 1998). Principally, Hsp70s consist of an Nterminal ATPase ...
A drug-controllable tag for visualizing newly synthesized proteins in
... tag whose presence on new proteins could be induced by the one-time administration of a small-molecule drug to either cells or animals. The strategy we devised was to incorporate a specific protease activity to confer self-removing behavior onto an epitope tag and then to use a corresponding proteas ...
... tag whose presence on new proteins could be induced by the one-time administration of a small-molecule drug to either cells or animals. The strategy we devised was to incorporate a specific protease activity to confer self-removing behavior onto an epitope tag and then to use a corresponding proteas ...
IF-3
... Eukaryotic 18S rRNA has additional domains. One end of the 30S subunit may consist largely or entirely of rRNA. ...
... Eukaryotic 18S rRNA has additional domains. One end of the 30S subunit may consist largely or entirely of rRNA. ...
Characterizing Protein/Ligand Binding by DSC
... change due to binding is calculated by subtracting the free energy change of unfolding of the complex (∆Gcomp) from the free energy change of unfolding of the protein (∆Gprot) (i.e., ∆G = ∆Gprot– ∆Gcomp). If the endotherm is complex, indicative of multiple folding states, the endotherm must be decon ...
... change due to binding is calculated by subtracting the free energy change of unfolding of the complex (∆Gcomp) from the free energy change of unfolding of the protein (∆Gprot) (i.e., ∆G = ∆Gprot– ∆Gcomp). If the endotherm is complex, indicative of multiple folding states, the endotherm must be decon ...
Autophagy_Marc
... cellular components through the actions of lysosomes . The breakdown of cellular components promotes cellular survival during starvation by maintaining cellular energy levels. Autophagy allows the degradation and recycling of cellular components. During this process, targeted cytoplasmic constituent ...
... cellular components through the actions of lysosomes . The breakdown of cellular components promotes cellular survival during starvation by maintaining cellular energy levels. Autophagy allows the degradation and recycling of cellular components. During this process, targeted cytoplasmic constituent ...
Exploring Proteins - Weber State University
... • Immunoglobulins (antibodies) can be utilized as powerful analytical tools in biochemistry • An antibody (Immunoglobulin, Ig) is a complex protein formed by an animal in response to the presence of a foreign substance (most often foreign proteins). • An antibody usually exhibits specific and high ...
... • Immunoglobulins (antibodies) can be utilized as powerful analytical tools in biochemistry • An antibody (Immunoglobulin, Ig) is a complex protein formed by an animal in response to the presence of a foreign substance (most often foreign proteins). • An antibody usually exhibits specific and high ...
Problem of Focus - Clarkson University
... Mer P is a periplasmic mercury binding protein present in some strains of bacteria that display resistance to mercury (II) ions. A periplasmic protein is a protein is located in the layer between the bacteria membrane and cell wall in the periplasmic layer. MerP is 7500 kd in weight, 72 amino acids ...
... Mer P is a periplasmic mercury binding protein present in some strains of bacteria that display resistance to mercury (II) ions. A periplasmic protein is a protein is located in the layer between the bacteria membrane and cell wall in the periplasmic layer. MerP is 7500 kd in weight, 72 amino acids ...
Protein microarrays
... gene products by large-scale genomic and proteomic initiatives has required the development of high-throughput strategies to elucidate their function [8]. There have primarily been two approaches to characterize multiple proteins in biological samples. The first approach, 2D-gels, has been widely us ...
... gene products by large-scale genomic and proteomic initiatives has required the development of high-throughput strategies to elucidate their function [8]. There have primarily been two approaches to characterize multiple proteins in biological samples. The first approach, 2D-gels, has been widely us ...
Seasonal regulation of a 24-kDa protein from red
... Total and CaCl2-extractable proteins were isolated from C. sericea wood throughout the year and analyzed by SDSPAGE (Figures 1 and 2). Among total protein extracts, the accumulation of a 24-kDa protein correlated with periods of cold acclimation. The 24-kDa protein was abundant in January and March, ...
... Total and CaCl2-extractable proteins were isolated from C. sericea wood throughout the year and analyzed by SDSPAGE (Figures 1 and 2). Among total protein extracts, the accumulation of a 24-kDa protein correlated with periods of cold acclimation. The 24-kDa protein was abundant in January and March, ...
GPS-Lipid Manual - CSS-Palm
... geranylgeranylation is performed by protein geranylgeranyltransferase type I (GGTase-I) (12,13). However, in case of Rab proteins, the geranylgeranyltransferase type II (GGTase-II) which recognized a C-terminal CC/CXC motif is found to catalyze the geranylgeranylation process (10,14). In addition to ...
... geranylgeranylation is performed by protein geranylgeranyltransferase type I (GGTase-I) (12,13). However, in case of Rab proteins, the geranylgeranyltransferase type II (GGTase-II) which recognized a C-terminal CC/CXC motif is found to catalyze the geranylgeranylation process (10,14). In addition to ...
Proteomics methods for subcellular proteome analysis
... allows accurate identification of proteins in the original sample, providing an inventory of the proteins that are present in the isolated organelle. For several compartments, such as the nucleus and the mitochondrion, biochemical enrichment is generally easier to achieve by disrupting cells under m ...
... allows accurate identification of proteins in the original sample, providing an inventory of the proteins that are present in the isolated organelle. For several compartments, such as the nucleus and the mitochondrion, biochemical enrichment is generally easier to achieve by disrupting cells under m ...
Signals and mechanisms for protein retention in the endoplasmic
... It was first thought that the ER-resident ERD receptors would bind the K/HDEL retention signal to divert reticuloplasmins from the bulk flow of secreted proteins. There is now some evidence, at least in mammals and yeast, that these receptors do not stricto sensu retain reticuloplasmins in the ER, b ...
... It was first thought that the ER-resident ERD receptors would bind the K/HDEL retention signal to divert reticuloplasmins from the bulk flow of secreted proteins. There is now some evidence, at least in mammals and yeast, that these receptors do not stricto sensu retain reticuloplasmins in the ER, b ...
Team Publications - Centre de recherche de l`Institut Curie
... The mammalian Polycomblike protein PHF1 was previously shown to interact with the Polycomb group (PcG) protein Ezh2, a histone methyltransferase whose activity is pivotal in sustaining gene repression during development and in adulthood. As Ezh2 is active only when part of the Polycomb Repressive Co ...
... The mammalian Polycomblike protein PHF1 was previously shown to interact with the Polycomb group (PcG) protein Ezh2, a histone methyltransferase whose activity is pivotal in sustaining gene repression during development and in adulthood. As Ezh2 is active only when part of the Polycomb Repressive Co ...
Glutathionylation in the Photosynthetic Model Organism
... under the reduced form (GSH) because oxidized glutathione (GSSG) is continuously regenerated into GSH by glutathione reductase using NADPH as electron donor. Glutathione has numerous documented physiological functions. It is generally considered to constitute a redox buffer and also participates in ...
... under the reduced form (GSH) because oxidized glutathione (GSSG) is continuously regenerated into GSH by glutathione reductase using NADPH as electron donor. Glutathione has numerous documented physiological functions. It is generally considered to constitute a redox buffer and also participates in ...
The P5 protein from bacteriophage phi
... Our analysis of sequence similarity searches and Cystovirus phage genomes suggests that the P5 protein from bacteriophage phi-6, as the only member of peptidase family U40, is homologous to the lytic transglycosylases and has a lysozyme-like fold. This prediction is consistent with the lytic functio ...
... Our analysis of sequence similarity searches and Cystovirus phage genomes suggests that the P5 protein from bacteriophage phi-6, as the only member of peptidase family U40, is homologous to the lytic transglycosylases and has a lysozyme-like fold. This prediction is consistent with the lytic functio ...
The RNA polymerase factory: a robotic in vitro assembly platform for
... these functional domains tend to be either inherently unstructured [e.g. Fork and Trigger Loops (2–4)] or are part of flexible a-helical structures [e.g. Bridge Helix (1,2) and Trigger Loop (8)] so that single amino acid substitutions are unlikely to interfere with subunit assembly or overall stabili ...
... these functional domains tend to be either inherently unstructured [e.g. Fork and Trigger Loops (2–4)] or are part of flexible a-helical structures [e.g. Bridge Helix (1,2) and Trigger Loop (8)] so that single amino acid substitutions are unlikely to interfere with subunit assembly or overall stabili ...
Two Structural Domains Mediate Two Sequential y-Zein
... those two domains. These mutants and the wild-type y-zein were expressed in Arabidopsis, and their subcellular distribution inside the cells was studied. A PvulXbal DNA fragment of a y-zein genomic clone (Boronat et ai., 1986) containing the y-zein coding sequence (669 bp), which is flanked by a sho ...
... those two domains. These mutants and the wild-type y-zein were expressed in Arabidopsis, and their subcellular distribution inside the cells was studied. A PvulXbal DNA fragment of a y-zein genomic clone (Boronat et ai., 1986) containing the y-zein coding sequence (669 bp), which is flanked by a sho ...
In Vitro Translation Systems – Protein expression
... Express up to 0.75mg per mL of functional protein in less than 16 hours. The 1-Step Human High-Yield In Vitro Translation (IVT) Kits enable the expression of functional proteins with 10 to 100 times greater yield per mL than other mammalian IVT systems. The 1-Step High-Yield IVT System uses modified ...
... Express up to 0.75mg per mL of functional protein in less than 16 hours. The 1-Step Human High-Yield In Vitro Translation (IVT) Kits enable the expression of functional proteins with 10 to 100 times greater yield per mL than other mammalian IVT systems. The 1-Step High-Yield IVT System uses modified ...
novel 4E-interacting protein in Leishmania is involved in stage
... and subjected to western blot analysis with specific antibodies against the different LeishIF4F subunits. Mass spectrometry analysis Proteins that were pulled down with the tagged LeishIF4E-1 of LeishIF4E-4 from L. amazonensis cell lines (3–4 109 cells) were precipitated by TCA and further resolved ...
... and subjected to western blot analysis with specific antibodies against the different LeishIF4F subunits. Mass spectrometry analysis Proteins that were pulled down with the tagged LeishIF4E-1 of LeishIF4E-4 from L. amazonensis cell lines (3–4 109 cells) were precipitated by TCA and further resolved ...
Plant organelle proteomics
... elucidate their role in the cell as proteins are spatially organised according to their function [4]. Secondly, it refines our knowledge of cellular processes by pinpointing certain activities to specific organelles [5]. Traditional methods to assign proteins to subcellular locations are mostly targ ...
... elucidate their role in the cell as proteins are spatially organised according to their function [4]. Secondly, it refines our knowledge of cellular processes by pinpointing certain activities to specific organelles [5]. Traditional methods to assign proteins to subcellular locations are mostly targ ...
Proteasome
Proteasomes are protein complexes inside all eukaryotes and archaea, and in some bacteria. The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds.