S1. Comparison of complex functions in MCL-GO
... are conserved in their biological processes in E. coli than yeast. Conversely fewer superfamilies are conserved in molecular function and cellular component in E. coli than yeast. Notice that the numbers are correlated with organismal complexity. The results suggest that more complex organisms have ...
... are conserved in their biological processes in E. coli than yeast. Conversely fewer superfamilies are conserved in molecular function and cellular component in E. coli than yeast. Notice that the numbers are correlated with organismal complexity. The results suggest that more complex organisms have ...
Transport of the precursor to neurospora ATPase
... Downloaded from www.jbc.org by on November 12, 2008 ...
... Downloaded from www.jbc.org by on November 12, 2008 ...
Chapter 9
... Many chaperones were initially identified as heat-shock proteins (Hsp), expressed in cells subjected to high temperatures. Hsp stabilize and facilitate refolding of proteins that have been partially denatured. ...
... Many chaperones were initially identified as heat-shock proteins (Hsp), expressed in cells subjected to high temperatures. Hsp stabilize and facilitate refolding of proteins that have been partially denatured. ...
Over Expression of IPTG inducible GST protein in E.coli BL21
... by glutathione on an electrophilic substrate [2]. The resulting glutathione S conjugate are more soluble than the original substrate and thus more easily transported from the cell, mediated by ATP dependent MAPEG family membrane glycoprotein belonging to multiple drug resistant protein family [3]. E ...
... by glutathione on an electrophilic substrate [2]. The resulting glutathione S conjugate are more soluble than the original substrate and thus more easily transported from the cell, mediated by ATP dependent MAPEG family membrane glycoprotein belonging to multiple drug resistant protein family [3]. E ...
Expression of Hepatitis C Virus Structural Proteins in
... 250 kDa protein was reacted with anti-E2, anti-E1, and anti-core antibodies (Figs. 3A, 3B and 3C, respectively). This large protein was assumed to be a nonprocessed aggregated form. The E1 protein was also detected as a slightly smeared form of 35 kDa in size by Western blot (Fig 3B). When we used m ...
... 250 kDa protein was reacted with anti-E2, anti-E1, and anti-core antibodies (Figs. 3A, 3B and 3C, respectively). This large protein was assumed to be a nonprocessed aggregated form. The E1 protein was also detected as a slightly smeared form of 35 kDa in size by Western blot (Fig 3B). When we used m ...
D - Protein Information Resource
... cleavage, and other post-translational modifications (ProForm—Protein Form ontology). PRO is designed to assist assignment of protein annotations (properties such as molecular functions) to specific protein forms of a gene product, and to protein classes at different evolutionary levels in a formal ...
... cleavage, and other post-translational modifications (ProForm—Protein Form ontology). PRO is designed to assist assignment of protein annotations (properties such as molecular functions) to specific protein forms of a gene product, and to protein classes at different evolutionary levels in a formal ...
Directed Proteomics Identifies a Plant
... AtPhos43 Is Phosphorylated in Planta Cell cultures provide an excellent biochemical system to study synchronized elicitor responses, but it is essential to connect these observations to responses in whole plants. To determine if AtPhos43 also is phosphorylated in response to elicitor treatment in pl ...
... AtPhos43 Is Phosphorylated in Planta Cell cultures provide an excellent biochemical system to study synchronized elicitor responses, but it is essential to connect these observations to responses in whole plants. To determine if AtPhos43 also is phosphorylated in response to elicitor treatment in pl ...
Reconstitution of Outer Membrane Protein Assembly from Purified
... SurA, and this chaperone-OmpT complex was diluted into solutions containing the proteoliposomes, the fluorogenic peptide, and lipopolysaccharide (LPS), which is required for OmpT activity (17). OmpT assembly was observed as an increase in the rate of fluorescence production. OmpT activity increased ...
... SurA, and this chaperone-OmpT complex was diluted into solutions containing the proteoliposomes, the fluorogenic peptide, and lipopolysaccharide (LPS), which is required for OmpT activity (17). OmpT assembly was observed as an increase in the rate of fluorescence production. OmpT activity increased ...
Heavy Metals and Metalloids As a Cause for Protein Misfolding and
... polypeptide under given conditions (particularly pH, temperature and the type and concentration of salt ions) apparently attain their final 3D structure essentially via the same transition states and intermediates. The rate of folding depends on the particular protein and varies in a wide range. Sma ...
... polypeptide under given conditions (particularly pH, temperature and the type and concentration of salt ions) apparently attain their final 3D structure essentially via the same transition states and intermediates. The rate of folding depends on the particular protein and varies in a wide range. Sma ...
Substrate Specificity Kit – In Brief
... original substrate. Test the new substrate to see if it will fit into your enzyme’s active site. Explain how your enzyme is specific to the substrate you have created. 6. Using your own substrate again, rotate the functional groups around the movable bond between the two gray spheres and try to dock ...
... original substrate. Test the new substrate to see if it will fit into your enzyme’s active site. Explain how your enzyme is specific to the substrate you have created. 6. Using your own substrate again, rotate the functional groups around the movable bond between the two gray spheres and try to dock ...
Information Retrieval Performance and Method
... resulting in a collection of 222,609 unique PubMed abstracts—4 times the size of PubMed abstracts that were retrieved with the simple query term “Alzheimer” against the same database. In this step, we were mainly interested in high recall—retrieving as many abstracts as the text mining system can re ...
... resulting in a collection of 222,609 unique PubMed abstracts—4 times the size of PubMed abstracts that were retrieved with the simple query term “Alzheimer” against the same database. In this step, we were mainly interested in high recall—retrieving as many abstracts as the text mining system can re ...
Roles of F-box Proteins in Plant Hormone Responses
... that are involved in the regulation of many physiological and developmental processes, including root growth, fruit ripening, senescence, pollen development, and adaptation to environmental stresses [49,50]. The F-box protein COI1 is a pivotal factor in the JA signal response [Fig. 3(D)] and is requ ...
... that are involved in the regulation of many physiological and developmental processes, including root growth, fruit ripening, senescence, pollen development, and adaptation to environmental stresses [49,50]. The F-box protein COI1 is a pivotal factor in the JA signal response [Fig. 3(D)] and is requ ...
Chaperone-assisted protein folding: the path to discovery from a
... a-helices and b-sheets, such as the TIM barrel, ...
... a-helices and b-sheets, such as the TIM barrel, ...
Insights from the HuR-interacting transcriptome: ncRNAs, ubiquitin
... extensive concentration of Ubiquitin Pathway genes among the HuR-positive transcripts and suggested that HuR not only affect the production of protein but also regulate the ubiqutin pathways of their selective degradation. In another way, the authors analyzed the number of natural antisense transcri ...
... extensive concentration of Ubiquitin Pathway genes among the HuR-positive transcripts and suggested that HuR not only affect the production of protein but also regulate the ubiqutin pathways of their selective degradation. In another way, the authors analyzed the number of natural antisense transcri ...
19-9-ET-V1-S1__preci..
... leads to competition for water molecule on the proteins. Completely ionized salts have more affinity for water molecules then protein hence addition of salts takes up water molecule from the protein. Therefore the ionic interactions between water molecules and protein are reduced and as result hydro ...
... leads to competition for water molecule on the proteins. Completely ionized salts have more affinity for water molecules then protein hence addition of salts takes up water molecule from the protein. Therefore the ionic interactions between water molecules and protein are reduced and as result hydro ...
Protein Quality Control as a Strategy for Cellular Regulation
... ubiquitin to an E2 ubiquitin-conjugating (UBC) enzyme. Ubiquitin is then transferred from the ubiquitin-charged E2 to the substrate or the growing ubiquitin chain by the action of an E3 ubiquitin ligase, resulting in a substrate-attached multiubiquitin chain that is recognized by the proteasome, lea ...
... ubiquitin to an E2 ubiquitin-conjugating (UBC) enzyme. Ubiquitin is then transferred from the ubiquitin-charged E2 to the substrate or the growing ubiquitin chain by the action of an E3 ubiquitin ligase, resulting in a substrate-attached multiubiquitin chain that is recognized by the proteasome, lea ...
Structure and Function of Salivary Proteins Outline Basic salivary
... • There are specific proteins (biomarkers) expressed in diseased or tumor tissues that are different from or absent in normal tissues ...
... • There are specific proteins (biomarkers) expressed in diseased or tumor tissues that are different from or absent in normal tissues ...
Wnt Signaling Translocates Lys48-Linked
... Wnt3a for 4 hr displayed an increase in total polyUb content in western blots using a monoclonal antibody (called FK1) that recognizes all possible forms of polyubiquitin chains (Figure 1A). The accumulation of polyUb was most striking between 30 and 120 min after addition of purified Wnt3a protein ...
... Wnt3a for 4 hr displayed an increase in total polyUb content in western blots using a monoclonal antibody (called FK1) that recognizes all possible forms of polyubiquitin chains (Figure 1A). The accumulation of polyUb was most striking between 30 and 120 min after addition of purified Wnt3a protein ...
Problem Set 5, 7.06, Spring 2003 1. In order to please your
... proteins, the nucleus, too. Everything was going fine, and you have tubes of protein extract from each of the organelles fresh out of the centrifuge and ready to be labelled (you're so good at the purification that you never label tubes until you are ready to put them in the freezer, but simply rely ...
... proteins, the nucleus, too. Everything was going fine, and you have tubes of protein extract from each of the organelles fresh out of the centrifuge and ready to be labelled (you're so good at the purification that you never label tubes until you are ready to put them in the freezer, but simply rely ...
REVIEWS
... Figure 1 | The effects of the endoplasmic reticulum environment on folding equilibria. The equilibrium between the native (N) and incompletely folded (I) forms of a protein (green) in the endoplasmic reticulum (ER) is affected by the ER environment, in particular by the presence of molecular chapero ...
... Figure 1 | The effects of the endoplasmic reticulum environment on folding equilibria. The equilibrium between the native (N) and incompletely folded (I) forms of a protein (green) in the endoplasmic reticulum (ER) is affected by the ER environment, in particular by the presence of molecular chapero ...
COP9 signalosome turns the key on protein degradation
... CSN as a regulator of protein turnover by the 26S proteasome A number of early or circumstantial pieces of evidence suggested that the CSN is a regulator of protein turnover. First, mutations in CSN genes inhibit the turnover of the Arabidopsis LONG HYPOCOTYL5 (HY5) and HY5 HOMOLOG (HYH) basic leuci ...
... CSN as a regulator of protein turnover by the 26S proteasome A number of early or circumstantial pieces of evidence suggested that the CSN is a regulator of protein turnover. First, mutations in CSN genes inhibit the turnover of the Arabidopsis LONG HYPOCOTYL5 (HY5) and HY5 HOMOLOG (HYH) basic leuci ...
Functional decorations: post-translational modifications and heart
... predominantly nucleocytoplasmic proteins. O-GlcNAc thus has the potential to compete with phosphorylation for binding sites [49]. This has led to the proposal of OGlcNAc-phosphate crosstalk (see below), although there are currently few examples of reciprocal regulation at identical sites and the fun ...
... predominantly nucleocytoplasmic proteins. O-GlcNAc thus has the potential to compete with phosphorylation for binding sites [49]. This has led to the proposal of OGlcNAc-phosphate crosstalk (see below), although there are currently few examples of reciprocal regulation at identical sites and the fun ...
Identification of NaHCO3 Stress Responsive Proteins in Dunaliella
... for CO2 fixation. There, however, are concerns over algae growth inhibition caused by high HCO3- concentration. The exact mechanism of the microalgae response to NaHCO3 stress also needs further understanding. This study evaluated the effect of high HCO3- on enhancing growth of algae Dunaliella sali ...
... for CO2 fixation. There, however, are concerns over algae growth inhibition caused by high HCO3- concentration. The exact mechanism of the microalgae response to NaHCO3 stress also needs further understanding. This study evaluated the effect of high HCO3- on enhancing growth of algae Dunaliella sali ...
Formation of Lys63-linked polyubiquitin chains on Gap1p permease
... promote both basal and ligand-induced internalization of a Cterminally truncated form of Ste2p (Terrel et al., 1998). Although ligand binding to full-length Ste2p leads to formation of di-and tri-ubiquitinated Ste2p conjugates, there is evidence that this may mainly reflect addition of single Ub moi ...
... promote both basal and ligand-induced internalization of a Cterminally truncated form of Ste2p (Terrel et al., 1998). Although ligand binding to full-length Ste2p leads to formation of di-and tri-ubiquitinated Ste2p conjugates, there is evidence that this may mainly reflect addition of single Ub moi ...
Proteasome
Proteasomes are protein complexes inside all eukaryotes and archaea, and in some bacteria. The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds.