A quantitative atlas of mitotic phosphorylation
... identification of >14,000 different phosphorylation events, more than half of which, to our knowledge, have not been described in the literature, along with relative quantitative data for the majority of these sites. We observed >1,000 proteins with increased phosphorylation in mitosis including man ...
... identification of >14,000 different phosphorylation events, more than half of which, to our knowledge, have not been described in the literature, along with relative quantitative data for the majority of these sites. We observed >1,000 proteins with increased phosphorylation in mitosis including man ...
Untitled
... Phe residues are sufficiently exposed to allow rapid binding of transport receptors. The aggregates in fact display an organization into parallel or antiparallel substructures, comprising between two and four monomers each, which are connected by β-bridges. Our simulations thus suggest that FG repea ...
... Phe residues are sufficiently exposed to allow rapid binding of transport receptors. The aggregates in fact display an organization into parallel or antiparallel substructures, comprising between two and four monomers each, which are connected by β-bridges. Our simulations thus suggest that FG repea ...
A General Target Selection Method for Crystallographic Proteomics
... correlations between crystallization success and protein properties predicted from sequence only (7-10). Target selection methods take advantage of the data generated by structural genomics projects to identify correlations between protein attributes (determined by sequence analysis) and its success ...
... correlations between crystallization success and protein properties predicted from sequence only (7-10). Target selection methods take advantage of the data generated by structural genomics projects to identify correlations between protein attributes (determined by sequence analysis) and its success ...
Research Article Identification and Characterization of Cell Wall
... to cell-cell recognition. The cell wall of dinoflagellates is a subcellular component of substantial interest with regard to various aspects of cell surface associated ecophysiology. However, there are few experimental data available for the cell wall of dinoflagellates compared with other organisms ...
... to cell-cell recognition. The cell wall of dinoflagellates is a subcellular component of substantial interest with regard to various aspects of cell surface associated ecophysiology. However, there are few experimental data available for the cell wall of dinoflagellates compared with other organisms ...
Effects of calf serum on pinocytosis and proteolysis in rat yolk sacs
... in the absence of serum was 355 & 27.9pI/mg of yolk-sac protein per h. Addition of scrum to the medium caused a relatively greater fall in the rate of pinocytosis as compared with 1251-PVP(Fig. 1); in the presence of 50% serum the rate fell to 5% of the rate in serum-free medium. The greater percent ...
... in the absence of serum was 355 & 27.9pI/mg of yolk-sac protein per h. Addition of scrum to the medium caused a relatively greater fall in the rate of pinocytosis as compared with 1251-PVP(Fig. 1); in the presence of 50% serum the rate fell to 5% of the rate in serum-free medium. The greater percent ...
AMPKA1B2G1 Master Q102-1
... with an equal volume of distilled H2O. Step 5. Initiate the reaction by the addition of 5 µl [33P]-ATP Assay Cocktail bringing the final volume up to 25µl and incubate the mixture in a water bath at 30oC for 15 minutes. Step 6. After the 15 minute incubation period, terminate the reaction by spottin ...
... with an equal volume of distilled H2O. Step 5. Initiate the reaction by the addition of 5 µl [33P]-ATP Assay Cocktail bringing the final volume up to 25µl and incubate the mixture in a water bath at 30oC for 15 minutes. Step 6. After the 15 minute incubation period, terminate the reaction by spottin ...
The role of structural disorder in cell cycle regulation, related clinical
... 53BP1 is important for the activation of cell cycle checkpoints [49,52]. MDC1 also interacts with several components of the APC/C through the FHA, tBRCT and PST repeat domains of MDC1 [53] Thus, by affecting APC/C activity, MDC1 may have a role in the normal transition of cells from metaphase to ana ...
... 53BP1 is important for the activation of cell cycle checkpoints [49,52]. MDC1 also interacts with several components of the APC/C through the FHA, tBRCT and PST repeat domains of MDC1 [53] Thus, by affecting APC/C activity, MDC1 may have a role in the normal transition of cells from metaphase to ana ...
Assembly and maintenance of the sarcomere night and day
... Titin is a central player in the assembly process. It is a multifunctional protein containing multiple binding sites for structural and signalling proteins, as well as an inherent kinase domain, and also serves as a molecular spring.16 The N-terminus of titin spans the Z-disc and contains several sm ...
... Titin is a central player in the assembly process. It is a multifunctional protein containing multiple binding sites for structural and signalling proteins, as well as an inherent kinase domain, and also serves as a molecular spring.16 The N-terminus of titin spans the Z-disc and contains several sm ...
Lecture 19 - phys.protres.ru
... must become more and more stable for hierarchic folding. This cannot provide a simultaneous explanation to (i) folding within non-astronomical time; (ii) “all-or-none” transition, i.e., co-existence of only native and denatured molecules in visible amount; (iii) the same 3D structure resulting from ...
... must become more and more stable for hierarchic folding. This cannot provide a simultaneous explanation to (i) folding within non-astronomical time; (ii) “all-or-none” transition, i.e., co-existence of only native and denatured molecules in visible amount; (iii) the same 3D structure resulting from ...
21 О О О Termination of Polypeptide Synthesis Requires a Stop
... elongation begin on free cytosolic ribosomes. Proteins of the secretory pathway have a hydrophobic signal peptide, usually at or near their amino terminus. There is no unique signal peptide sequence, but its characteristics include a positively charged N terminus, a core of 8–12 hydrophobic amino ac ...
... elongation begin on free cytosolic ribosomes. Proteins of the secretory pathway have a hydrophobic signal peptide, usually at or near their amino terminus. There is no unique signal peptide sequence, but its characteristics include a positively charged N terminus, a core of 8–12 hydrophobic amino ac ...
Nuclear Localization and Interaction with COP1 Are Required for
... BBX24 interacts with COP1 in the yeast two-hybrid system (Holm et al., 2002) and colocalizes with it in plant cells (Indorf et al., 2007). It contains two B-box Zn fingers situated in tandem in the N-terminal part of the protein, whereas at the C terminus, several amino acid residues necessary for t ...
... BBX24 interacts with COP1 in the yeast two-hybrid system (Holm et al., 2002) and colocalizes with it in plant cells (Indorf et al., 2007). It contains two B-box Zn fingers situated in tandem in the N-terminal part of the protein, whereas at the C terminus, several amino acid residues necessary for t ...
Regulation of Cell Cycle
... Mammals-several Cdks (classical model), Cdk1 is essential to drive cell cycle in the absence of other Cdk (mouse knock out model) ...
... Mammals-several Cdks (classical model), Cdk1 is essential to drive cell cycle in the absence of other Cdk (mouse knock out model) ...
De Robertis 1.pm
... CR domains of the type present in Chordin are found in many other extracellular space proteins. These include the fibrillar procollagens (type I, III and V), amnionless, neuralin-1 and 2, CRIM-1, crossveinless-2 and CTGF. Neuralins contain three cysteine-rich domains and can bind Tsg, which promotes ...
... CR domains of the type present in Chordin are found in many other extracellular space proteins. These include the fibrillar procollagens (type I, III and V), amnionless, neuralin-1 and 2, CRIM-1, crossveinless-2 and CTGF. Neuralins contain three cysteine-rich domains and can bind Tsg, which promotes ...
Theoretical studies of viral capsid proteins
... of protein–protein contacts is necessary, as genetic economy limits the virus to relatively few gene products. This variation raises questions related to the specific recognition of different contact interfaces and the accuracy of assembling multiple protein copies into the proper capsid shell. A fi ...
... of protein–protein contacts is necessary, as genetic economy limits the virus to relatively few gene products. This variation raises questions related to the specific recognition of different contact interfaces and the accuracy of assembling multiple protein copies into the proper capsid shell. A fi ...
The NF- B Pathway
... adhesion molecules and inducible enzymes such as cycloxygenase-2 and inducible nitric oxide synthase, which regulate the innate immune response, as well as proteins that regulate the specific immune response, such as major ...
... adhesion molecules and inducible enzymes such as cycloxygenase-2 and inducible nitric oxide synthase, which regulate the innate immune response, as well as proteins that regulate the specific immune response, such as major ...
A Few Good Domains
... Phosphotyrosine binding (PTB) domains are 100–150 residue modules that commonly bind Asn-Pro-X-Tyr motifs. The PTB domains of the docking proteins Shc and IRS-1 require ligand phosphorylation on the tyrosine residue (NPXpY) for binding. More Nterminal sequences are also required for high affinity bi ...
... Phosphotyrosine binding (PTB) domains are 100–150 residue modules that commonly bind Asn-Pro-X-Tyr motifs. The PTB domains of the docking proteins Shc and IRS-1 require ligand phosphorylation on the tyrosine residue (NPXpY) for binding. More Nterminal sequences are also required for high affinity bi ...
Human/Mouse/Rat PP2A Catalytic Subunit Antibody
... BACKGROUND Protein Phosphatase 2A (PP2A) dephosphorylates serine and threonine residues in proteins. This ubiquitously expressed enzyme plays a critical role in modulating cell survival, growth factor responses, and neurotransmission. Phosphorylation near the Cterminus at Y307 of the catalytic su ...
... BACKGROUND Protein Phosphatase 2A (PP2A) dephosphorylates serine and threonine residues in proteins. This ubiquitously expressed enzyme plays a critical role in modulating cell survival, growth factor responses, and neurotransmission. Phosphorylation near the Cterminus at Y307 of the catalytic su ...
Novel Ubiquitin Fusion Proteins: Ribosomal Protein
... ubiquitin-ribosomal protein S27a fusion and the amino acid sequence of the region flanking the ubiquitin-S27a boundary (roughly corresponding to the filled bar in the schematic). For comparison, the sequence is aligned with homologs from animals, fungi, and plants. (b) Ubiquitin-ribosomal protein L4 ...
... ubiquitin-ribosomal protein S27a fusion and the amino acid sequence of the region flanking the ubiquitin-S27a boundary (roughly corresponding to the filled bar in the schematic). For comparison, the sequence is aligned with homologs from animals, fungi, and plants. (b) Ubiquitin-ribosomal protein L4 ...
Bacteria-host relationship: ubiquitin ligases as weapons of
... Ub. Nevertheless, some bacteria, such as Mycobacterium tuberculosis, harbor proteasomes and degrade their own protein substrates [21]. It is established that Mycobacterium possesses a functional analogue of the Ub system known as the prokaryotic Ub-like protein (Pup) system [22]. During pupylation, ...
... Ub. Nevertheless, some bacteria, such as Mycobacterium tuberculosis, harbor proteasomes and degrade their own protein substrates [21]. It is established that Mycobacterium possesses a functional analogue of the Ub system known as the prokaryotic Ub-like protein (Pup) system [22]. During pupylation, ...
The cytoplasm of living cells: a functional mixture of thousands of
... What is in it? A concentrated solution of macromolecules, predominantly protein, but also RNA and in the case of prokaryotes one or a few huge DNA molecules. Proteins are heteropolymers; they are linear chains of amino acids that are typically folded up into a compact, relatively rigid native state ...
... What is in it? A concentrated solution of macromolecules, predominantly protein, but also RNA and in the case of prokaryotes one or a few huge DNA molecules. Proteins are heteropolymers; they are linear chains of amino acids that are typically folded up into a compact, relatively rigid native state ...
MyoD as a gatekeeper to cell cycle progression
... a phosphorylation mutant of MyoD, the authors observed MyoD loses its affinity for P/CAF upon phosphorylation and instead prefers binding to histone deacetylase 1 (HDAC1), a negative regulator of gene expression. Loss of MyoD phosphorylation resulted in delayed entrance into mitosis. The authors pro ...
... a phosphorylation mutant of MyoD, the authors observed MyoD loses its affinity for P/CAF upon phosphorylation and instead prefers binding to histone deacetylase 1 (HDAC1), a negative regulator of gene expression. Loss of MyoD phosphorylation resulted in delayed entrance into mitosis. The authors pro ...
Class: Protein functional Annotation and Family Classification
... Many have unknown functions Large-scale functional annotation of these sequences based simply on BLAST best hit has pitfalls; results are far from perfect Manual annotation of individual proteins is not efficient ...
... Many have unknown functions Large-scale functional annotation of these sequences based simply on BLAST best hit has pitfalls; results are far from perfect Manual annotation of individual proteins is not efficient ...
Proteasome
Proteasomes are protein complexes inside all eukaryotes and archaea, and in some bacteria. The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds.