The Bcl-3 oncoprotein acts as a bridging factor between NF
... Tip60, Bard1 or Jab1 together with p50 and Bcl-3 in Drosophila SL2 cells and assayed activation of a Pselectin promoter-luciferase reporter. A cis-regulating NF-kB site in this promoter is preferentially bound by p50 or p52 and can be activated by p50-Bcl-3 in BAEC or SL2 cells (Hirano et al., 1998; ...
... Tip60, Bard1 or Jab1 together with p50 and Bcl-3 in Drosophila SL2 cells and assayed activation of a Pselectin promoter-luciferase reporter. A cis-regulating NF-kB site in this promoter is preferentially bound by p50 or p52 and can be activated by p50-Bcl-3 in BAEC or SL2 cells (Hirano et al., 1998; ...
Proteomics of spermatogenesis: from protein lists to understanding
... and identified 362 differential protein spots corresponding to 257 different proteins involved in the initiation of mouse spermatogenesis. Furthermore, cluster analysis revealed six expression patterns, and bioinformatics analysis revealed that each pattern was related to many specific cell processe ...
... and identified 362 differential protein spots corresponding to 257 different proteins involved in the initiation of mouse spermatogenesis. Furthermore, cluster analysis revealed six expression patterns, and bioinformatics analysis revealed that each pattern was related to many specific cell processe ...
Supplementary Table VI. List of Deletions/Hypomorphic Alleles
... Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to G ...
... Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to G ...
- blogs@NTU
... The SYP61 proteome includes a complete SNARE complex and its regulatory proteins During vesicle fusion, syntaxins recognize and assemble in complexes with two other SNARE proteins on the target membrane, forming a three-helix bundle (t-SNARE complex), which interacts with a fourth SNARE on the vesic ...
... The SYP61 proteome includes a complete SNARE complex and its regulatory proteins During vesicle fusion, syntaxins recognize and assemble in complexes with two other SNARE proteins on the target membrane, forming a three-helix bundle (t-SNARE complex), which interacts with a fourth SNARE on the vesic ...
Degradation by bacteria of membrane and soluble protein in seawater
... other experiments, except that a lag period was observed during the initial 6 h in Expt 1 (data not shown). When degradation was linear, the r2 for the linear regression of ln(100 - % degradation) against time was 0.95 to 0.99 (Fig. lB, Table 3). It is notable that degradation rates of soluble and m ...
... other experiments, except that a lag period was observed during the initial 6 h in Expt 1 (data not shown). When degradation was linear, the r2 for the linear regression of ln(100 - % degradation) against time was 0.95 to 0.99 (Fig. lB, Table 3). It is notable that degradation rates of soluble and m ...
Lysine Acetylation - Regulator of Diverse Cellular Processes
... assures the traction forces required for muscle contraction and also for cytoplasmic streaming in non-muscle cells. All three actin isoforms can be acetylated5,7. Also, several regulatory proteins of the actin cytoskeleton are modified by acetylation. For example, several subunits of the Arp2/3 comp ...
... assures the traction forces required for muscle contraction and also for cytoplasmic streaming in non-muscle cells. All three actin isoforms can be acetylated5,7. Also, several regulatory proteins of the actin cytoskeleton are modified by acetylation. For example, several subunits of the Arp2/3 comp ...
Protein Quality Control along the Route to the Plant Vacuole
... ER resident proteins, whose roles are to assist correct structural maturation, seem to be important in the process of ER retention. Proteins that fail to reach the correct maturation because of permanent structural defects are eventually degraded by the system, in some cases via retrotranslocation f ...
... ER resident proteins, whose roles are to assist correct structural maturation, seem to be important in the process of ER retention. Proteins that fail to reach the correct maturation because of permanent structural defects are eventually degraded by the system, in some cases via retrotranslocation f ...
Sec14p-like proteins regulate phosphoinositide homoeostasis and
... Sec9p t-SNARE (target membrane soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) function Stt4p and Mss4p reside in the yeast plasma membrane [22]. This raises the possibility that SFH proteins modulate exocytic and/or endocytic events at the plasma membrane. To investig ...
... Sec9p t-SNARE (target membrane soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) function Stt4p and Mss4p reside in the yeast plasma membrane [22]. This raises the possibility that SFH proteins modulate exocytic and/or endocytic events at the plasma membrane. To investig ...
Protein aggregation in bacteria: the thin boundary
... aggregation is driven by a balance between protein intrinsic parameters such as hydrophobicity, electrostatics and bsheet propensity and extrinsic factors (Chiti & Dobson, 2009) such as temperature, pH, ionic strength and protein concentration (Sarell et al., 2013). Despite their heterogeneity and s ...
... aggregation is driven by a balance between protein intrinsic parameters such as hydrophobicity, electrostatics and bsheet propensity and extrinsic factors (Chiti & Dobson, 2009) such as temperature, pH, ionic strength and protein concentration (Sarell et al., 2013). Despite their heterogeneity and s ...
Whey to isolate - Proteinfactory
... “In comparison to other protein isolates, WPI has a distinct advantage in amino-acid profile because whey proteins are higher in essential amino acids, especially branched-chain and sulfur-containing amino acids,” says Bastain. “WPI customers should review the amino-acid profile of protein isolates ...
... “In comparison to other protein isolates, WPI has a distinct advantage in amino-acid profile because whey proteins are higher in essential amino acids, especially branched-chain and sulfur-containing amino acids,” says Bastain. “WPI customers should review the amino-acid profile of protein isolates ...
Forces Produced by Protofilament Curls Nucleotide Preference for End Binding Proteins
... • GTPγS microtubules had brighter tips than GTP microtubules • EB3 showed the greatest binding affinity at the tip for both microtubule types • EB2 showed the greatest binding affinity for GTPγS microtubul ...
... • GTPγS microtubules had brighter tips than GTP microtubules • EB3 showed the greatest binding affinity at the tip for both microtubule types • EB2 showed the greatest binding affinity for GTPγS microtubul ...
Fluorescent peptides as molecular probes
... HDAC Assay Kit, the longer-wavelength substrate shows significantly higher sensitivity than the AMC substrate (Figs 2 and 3). FRET peptides are peptides that contain a donor (fluorophore) and an acceptor (another fluorophore or a quencher). The donor molecule is usually placed on one terminus and a ...
... HDAC Assay Kit, the longer-wavelength substrate shows significantly higher sensitivity than the AMC substrate (Figs 2 and 3). FRET peptides are peptides that contain a donor (fluorophore) and an acceptor (another fluorophore or a quencher). The donor molecule is usually placed on one terminus and a ...
Lecture 8
... proteins that bind to the column. The greater the binding affinity of a protein for the ion exchange column, the more it will be slowed in eluting off the column. Proteins can be eluted by changing the elution buffer to one with a higher salt concentration and/or a different pH (stepwise elution or ...
... proteins that bind to the column. The greater the binding affinity of a protein for the ion exchange column, the more it will be slowed in eluting off the column. Proteins can be eluted by changing the elution buffer to one with a higher salt concentration and/or a different pH (stepwise elution or ...
Centrosomes as Scaffolds - Albert Einstein College of Medicine
... (1983) identified a 220 kDa protein that by immunofluorescence localized to the centrosome. This protein, named pericentrin, was found to form an integral part of centrosomes and non-centriolar MTOCs (Doxsey et al., 1994). The amino acid sequence of pericentrin predicts a tripartite structure with a ...
... (1983) identified a 220 kDa protein that by immunofluorescence localized to the centrosome. This protein, named pericentrin, was found to form an integral part of centrosomes and non-centriolar MTOCs (Doxsey et al., 1994). The amino acid sequence of pericentrin predicts a tripartite structure with a ...
A.G. Scientific, Inc. Protease Inhibitor Cocktail VI, Plant Cells
... Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of quickly degrading the proteins of interest present in the extract. As a result, this biochemical process can drastically reduce the yield of any protein during any isolation step and ...
... Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of quickly degrading the proteins of interest present in the extract. As a result, this biochemical process can drastically reduce the yield of any protein during any isolation step and ...
Caldicellulosiruptor tāpirins bind to crystalline cellulose! ! 1 Discrete
... from lignocellulosic substrates has intensified focus on the mechanisms by which microorganisms degrade and utilize plant biomass. To date, most attention has been focused on cellulolytic enzymes implicated in this process, but it has been established for some time that non-catalytic, biomolecular c ...
... from lignocellulosic substrates has intensified focus on the mechanisms by which microorganisms degrade and utilize plant biomass. To date, most attention has been focused on cellulolytic enzymes implicated in this process, but it has been established for some time that non-catalytic, biomolecular c ...
Synapse Specificity Minireview and Long
... indicating that in principal the individual neuron can process and store many bits of information. Electrophysiological experiments have also revealed that anatomically isolated groups of synapses on the same cell can separately process plastic events. Finally, many studies of behavioral and synapti ...
... indicating that in principal the individual neuron can process and store many bits of information. Electrophysiological experiments have also revealed that anatomically isolated groups of synapses on the same cell can separately process plastic events. Finally, many studies of behavioral and synapti ...
Materials and Methods - UROP
... concentration of 1 mM, and allowed to grow for another four hours. The cultures were then centrifuged at 6 krpm for 15 minutes and stored at -80 °C. The pellet was resuspended in 40 ml of binding buffer (5 mM imidazole, 500 mM NaCl, 20 mM Tris-HCl (pH7.5)). The bacterial suspension was sonicated and ...
... concentration of 1 mM, and allowed to grow for another four hours. The cultures were then centrifuged at 6 krpm for 15 minutes and stored at -80 °C. The pellet was resuspended in 40 ml of binding buffer (5 mM imidazole, 500 mM NaCl, 20 mM Tris-HCl (pH7.5)). The bacterial suspension was sonicated and ...
Nuclear Factor-90 of Activated T-Cells: A Double
... T-cells (NFATs),1 appear to be essential for early T-cell gene activation (1-6). The transcriptional activation of interleukin 2 (IL-2), the first lymphokine secreted following T-cell activation (7), likely involves NFATs as well as other transcription factors including AP-1, NF-κB, and Oct-1 (8, 9) ...
... T-cells (NFATs),1 appear to be essential for early T-cell gene activation (1-6). The transcriptional activation of interleukin 2 (IL-2), the first lymphokine secreted following T-cell activation (7), likely involves NFATs as well as other transcription factors including AP-1, NF-κB, and Oct-1 (8, 9) ...
Chapt. 10: Protein Sorting, Transport: Endoplasmic Reticulum, Golgi
... Posttranslation translocation (more common in yeast): • Proteins synthesized on free ribosomes • Signal sequences recognized by receptors on translocon (not need SRP) • Hsp70 chaperones keep polypeptide chains unfolded so can enter translocon • Hsp70 chaperone in ER (BiP) acts as ratchet to pull pol ...
... Posttranslation translocation (more common in yeast): • Proteins synthesized on free ribosomes • Signal sequences recognized by receptors on translocon (not need SRP) • Hsp70 chaperones keep polypeptide chains unfolded so can enter translocon • Hsp70 chaperone in ER (BiP) acts as ratchet to pull pol ...
Protein degradation in mitochondria
... larger high molecular mass assembly in vivo.28 Several domains can be distinguished in subunits of Lon-like proteases. They harbour an ATPase domain characteristic of Walker-type P-loop ATPases which exhibits a tertiary fold similar to other ATP-dependent proteases.29, 30 ATP hydrolysis is indispens ...
... larger high molecular mass assembly in vivo.28 Several domains can be distinguished in subunits of Lon-like proteases. They harbour an ATPase domain characteristic of Walker-type P-loop ATPases which exhibits a tertiary fold similar to other ATP-dependent proteases.29, 30 ATP hydrolysis is indispens ...
ppt
... Evidence for signal sequences for ER: Secretory proteins translated from mRNAs on free ribosomes are larger than the secreted proteins. With microsomes, growing polypeptide chains incorporated into microsomes, signal sequences removed by proteolytic cleavage. ...
... Evidence for signal sequences for ER: Secretory proteins translated from mRNAs on free ribosomes are larger than the secreted proteins. With microsomes, growing polypeptide chains incorporated into microsomes, signal sequences removed by proteolytic cleavage. ...
Dr Asmat Salim MM707-electrophoresis 2014
... Critical Parameters and Troubleshooting • It is important to use only high-quality electrophoresis-grade reagents when running the gels. Acrylamide and bisacrylamide both break down in solution to acrylic acid, which affects the mobility of molecules through the gel matrix. • Acrylamide solutions s ...
... Critical Parameters and Troubleshooting • It is important to use only high-quality electrophoresis-grade reagents when running the gels. Acrylamide and bisacrylamide both break down in solution to acrylic acid, which affects the mobility of molecules through the gel matrix. • Acrylamide solutions s ...
Interpretation of bafilomycin, pH neutralizing or protease inhibitor
... of lysosomal protease inhibitors (such as leupeptin), intralysosomal pH neutralizing agents (such as chloroquine), or drugs that inhibit the vacuolar type H+ -ATPase (v-ATPase) complex necessary for lysosomal acidification (such as bafilomycin A1). These treatments block autophagic degradation at a ...
... of lysosomal protease inhibitors (such as leupeptin), intralysosomal pH neutralizing agents (such as chloroquine), or drugs that inhibit the vacuolar type H+ -ATPase (v-ATPase) complex necessary for lysosomal acidification (such as bafilomycin A1). These treatments block autophagic degradation at a ...
Proteasome
Proteasomes are protein complexes inside all eukaryotes and archaea, and in some bacteria. The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds.