7.12. PROTEIN FOLDING AND MISFOLDING43
... potential heterogeneity of folding trajectories of individual polypeptides within the ensemble. Protein folding-unfolding reactions give important insights into the factors stabilizing (and destabilizing) the native form of protein. They shed light on the question of the mechanism of the assembly of ...
... potential heterogeneity of folding trajectories of individual polypeptides within the ensemble. Protein folding-unfolding reactions give important insights into the factors stabilizing (and destabilizing) the native form of protein. They shed light on the question of the mechanism of the assembly of ...
Computational Biology
... lines between boxes represent a one-bit difference between the two profiles. Homologous proteins are connected by a dashed line or are indented. Each protein is labeled by a four-digit E. coli gene number, a SwissProt gene name, and a brief description. Note that proteins within a box or in boxes co ...
... lines between boxes represent a one-bit difference between the two profiles. Homologous proteins are connected by a dashed line or are indented. Each protein is labeled by a four-digit E. coli gene number, a SwissProt gene name, and a brief description. Note that proteins within a box or in boxes co ...
Protein translocation across mitochondrial membranes
... of MOM72 with bound precursor or crosslinking of precursor with h.10M19(2’52Yi. A 32 kDa protein of yeast mitochondria (P32) was identified as a putative protein import receptor by employing anti-idiotypic antibodies that were raised against a synthetic mitochondrial presequence(*’). Surprisingly th ...
... of MOM72 with bound precursor or crosslinking of precursor with h.10M19(2’52Yi. A 32 kDa protein of yeast mitochondria (P32) was identified as a putative protein import receptor by employing anti-idiotypic antibodies that were raised against a synthetic mitochondrial presequence(*’). Surprisingly th ...
Journal of Bacteriology
... protein is exposed to the periplasmic side of the outer membrane and thus that the amino terminus of the PhoE protein part of the hybrid molecule faces the periplasm. However, an alternative possibility should be considered, i.e., the first residue of the PhoE protein part could be on the outside su ...
... protein is exposed to the periplasmic side of the outer membrane and thus that the amino terminus of the PhoE protein part of the hybrid molecule faces the periplasm. However, an alternative possibility should be considered, i.e., the first residue of the PhoE protein part could be on the outside su ...
Text S1 Y2H Interactome Mapping The literature was curated to
... found in CF by multiple studies; 75% of the eliminated ORFs encode proteins that were identified in CF in only a single proteomic study, in comparison to those that remained for which 32% were detected in only a single study. From the final list, 339 ORFs that were sequence validated were provided b ...
... found in CF by multiple studies; 75% of the eliminated ORFs encode proteins that were identified in CF in only a single proteomic study, in comparison to those that remained for which 32% were detected in only a single study. From the final list, 339 ORFs that were sequence validated were provided b ...
Regulatory assembly of the vacuolar proton pump VOV1
... to monitor the variable subunit composition of VOV1-ATPase parts. VOV1-ATPase shows a remarkable dis- and re-assembling process in response to nutrition conditions. In order to gain detailed information about this process we focused on subunits C and E of the V1 part. Different fluorescent proteins ...
... to monitor the variable subunit composition of VOV1-ATPase parts. VOV1-ATPase shows a remarkable dis- and re-assembling process in response to nutrition conditions. In order to gain detailed information about this process we focused on subunits C and E of the V1 part. Different fluorescent proteins ...
HiPer® Affinity Chromatography Teaching Kit
... Affinity chromatography is a very effective molecular technique for purification of protein on the basis of its biological function. Through this chromatography the desired protein is isolated from a mixed solution depending upon the protein's specific binding affinity to ligands mounted in a gel ma ...
... Affinity chromatography is a very effective molecular technique for purification of protein on the basis of its biological function. Through this chromatography the desired protein is isolated from a mixed solution depending upon the protein's specific binding affinity to ligands mounted in a gel ma ...
Supplementary Information
... (1) on a Silicon Graphics workstation (SGI, Sunnyvale, USA). Backbone atom superimpositions and figures were prepared with the program MOLMOL (2). 3D structures for p53 are available for several p53 stretches and domains. However, a structure of the region between amino acids 61 and 93 is missing, p ...
... (1) on a Silicon Graphics workstation (SGI, Sunnyvale, USA). Backbone atom superimpositions and figures were prepared with the program MOLMOL (2). 3D structures for p53 are available for several p53 stretches and domains. However, a structure of the region between amino acids 61 and 93 is missing, p ...
Generation of polyclonal antiserum for the detection
... P. Duan et al. / Journal of Immunological Methods 320 (2007) 132–142 ...
... P. Duan et al. / Journal of Immunological Methods 320 (2007) 132–142 ...
- Wiley Online Library
... assays. Our results showed that the maize protein MA16 is an RNA-binding protein that binds preferentially to uridine and guanosine-rich RNAs (Ludevid et al., 1992). Regulation studies indicated that MA16 mRNA had a basal level of expression in several tissues including embryos and seedlings. We als ...
... assays. Our results showed that the maize protein MA16 is an RNA-binding protein that binds preferentially to uridine and guanosine-rich RNAs (Ludevid et al., 1992). Regulation studies indicated that MA16 mRNA had a basal level of expression in several tissues including embryos and seedlings. We als ...
Protein Purification 2003
... • Enzyme clears lactic acid from working muscles • The obvious source of enzyme is muscle tissue (heart & skeletal muscle, H&M, isomers) • We will assay for the enzymes ability to convert Pyruvate to Lactate ...
... • Enzyme clears lactic acid from working muscles • The obvious source of enzyme is muscle tissue (heart & skeletal muscle, H&M, isomers) • We will assay for the enzymes ability to convert Pyruvate to Lactate ...
Teaching old NCATs new tricks: using non
... tag bearing the respective alkyne or azide moiety (R2). Newly synthesized, affinity tagged proteins can be quantified on immunoblots, affinity purified and subsequently identified via tandem-MS. Fluorescently labeled proteins, instead, enable visualization of protein synthesis dynamics in vivo and d ...
... tag bearing the respective alkyne or azide moiety (R2). Newly synthesized, affinity tagged proteins can be quantified on immunoblots, affinity purified and subsequently identified via tandem-MS. Fluorescently labeled proteins, instead, enable visualization of protein synthesis dynamics in vivo and d ...
the cell cycle in action - Oxford Academic
... finger (CUL3-BTB/POZ); UV-damaged DNA binding protein 1 (CUL4-DDB1) and the anaphase-promoting complex/ cyclosome (APC/C). In the case of plants, the number of E3 ligases is much higher than in other eukaryotes (yeast and mammals for example), also suggesting a broader number of targets that might b ...
... finger (CUL3-BTB/POZ); UV-damaged DNA binding protein 1 (CUL4-DDB1) and the anaphase-promoting complex/ cyclosome (APC/C). In the case of plants, the number of E3 ligases is much higher than in other eukaryotes (yeast and mammals for example), also suggesting a broader number of targets that might b ...
Exploration of the Dynamic Properties of Protein Complexes
... depletion of a single protein can alter the stability and activity of an entire protein complex. Experimental approaches that detect interactions within living cells provide critical information for the dynamical system that protein complexes represent; yet complexes are often depicted as static 2-d ...
... depletion of a single protein can alter the stability and activity of an entire protein complex. Experimental approaches that detect interactions within living cells provide critical information for the dynamical system that protein complexes represent; yet complexes are often depicted as static 2-d ...
ERdj5, an Endoplasmic Reticulum (ER)
... ORF, open reading frame; GFP, green fluorescent protein. This paper is available on line at http://www.jbc.org ...
... ORF, open reading frame; GFP, green fluorescent protein. This paper is available on line at http://www.jbc.org ...
BIOLOGY
... combinations of structural elements formed by the folding of nearby amino acid sequences. An example of a motif is a helix-loop-helix, which binds to DNA. Very similar motifs are found in many proteins that are not related. Scientists have classified conserved domains and motifs in a number of datab ...
... combinations of structural elements formed by the folding of nearby amino acid sequences. An example of a motif is a helix-loop-helix, which binds to DNA. Very similar motifs are found in many proteins that are not related. Scientists have classified conserved domains and motifs in a number of datab ...
Characterization of a Regular Array in the Wall of
... arranged subunits were isolated from the wall fraction of L. buchneri by extraction with 4 M-guanidine hydrochloride (GHCl) as described previously (Masuda & Kawata, 1980). The resulting fraction of wall fragments free of the subunits is referred to as GHC1-wall. Extraction of wall components. The G ...
... arranged subunits were isolated from the wall fraction of L. buchneri by extraction with 4 M-guanidine hydrochloride (GHCl) as described previously (Masuda & Kawata, 1980). The resulting fraction of wall fragments free of the subunits is referred to as GHC1-wall. Extraction of wall components. The G ...
Surveying Protein Structure and Function Using Bis
... binding to a linear amino acid sequence, Cys-Cys-Arg-Glu-Cys-Cys. Later work demonstrated that substituting Pro-Gly for Arg-Glu optimized both binding and fluorescence yield. Our strategy of bipartite tetracysteine display emanated from the idea that it would be possible to replace the intervening P ...
... binding to a linear amino acid sequence, Cys-Cys-Arg-Glu-Cys-Cys. Later work demonstrated that substituting Pro-Gly for Arg-Glu optimized both binding and fluorescence yield. Our strategy of bipartite tetracysteine display emanated from the idea that it would be possible to replace the intervening P ...
Electrophoretic_techniques2003
... A pure protein should give a single band on an SDS-poly acrylamide gel, unless the molecule is made of two unequal subunits. In the latter case two bands, corresponding to the two subunits will be seen Since only submicrogram amount of protein are needed for the gel. ...
... A pure protein should give a single band on an SDS-poly acrylamide gel, unless the molecule is made of two unequal subunits. In the latter case two bands, corresponding to the two subunits will be seen Since only submicrogram amount of protein are needed for the gel. ...
PRO
... Provides formalization to support precise annotation of specific protein classes/forms/complexes, allowing accurate data mapping, integration, analysis Allows specification of relationships between PRO and other ontologies, such as GO, SO (Sequence Ontology), PSI-MOD, ChEBI, CL (Cell Ontology) Provi ...
... Provides formalization to support precise annotation of specific protein classes/forms/complexes, allowing accurate data mapping, integration, analysis Allows specification of relationships between PRO and other ontologies, such as GO, SO (Sequence Ontology), PSI-MOD, ChEBI, CL (Cell Ontology) Provi ...
Sure, some prions can cause diseases, but others are
... HHMI scientists have begun to decipher another prion riddle— the existence of distinct “strains” of prions that produce different phenotypes. For example, the PrPSc prion causes several distinct neurological diseases in animals and humans. Each disease is slightly different in the length of its incu ...
... HHMI scientists have begun to decipher another prion riddle— the existence of distinct “strains” of prions that produce different phenotypes. For example, the PrPSc prion causes several distinct neurological diseases in animals and humans. Each disease is slightly different in the length of its incu ...
glycosphingolipid degradation - Limes-Institut-Bonn
... that the affected storage vesicles are in the late endosomal or lysosomal compartments. These compartments are still functionally active, except that they are unable to degrade sphingolipids that have short oligosaccharide head groups. After complementation of the medium of these cells with the miss ...
... that the affected storage vesicles are in the late endosomal or lysosomal compartments. These compartments are still functionally active, except that they are unable to degrade sphingolipids that have short oligosaccharide head groups. After complementation of the medium of these cells with the miss ...
Review Article
... Soluble proteins of the ER in animal cells share a common C-terminal tetrapeptide sequence, usually LysAsp-Glu-Leu (KDEL) that is essential for retention in the ER (Munro & Pelham, 1987). Variations on the KDEL theme are found in different organisms but the principles for function are no doubt very ...
... Soluble proteins of the ER in animal cells share a common C-terminal tetrapeptide sequence, usually LysAsp-Glu-Leu (KDEL) that is essential for retention in the ER (Munro & Pelham, 1987). Variations on the KDEL theme are found in different organisms but the principles for function are no doubt very ...
Proteasome
Proteasomes are protein complexes inside all eukaryotes and archaea, and in some bacteria. The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds.