Class: Protein functional Annotation and Family Classification
... Many have unknown functions Large-scale functional annotation of these sequences based simply on BLAST best hit has pitfalls; results are far from perfect Manual annotation of individual proteins is not efficient ...
... Many have unknown functions Large-scale functional annotation of these sequences based simply on BLAST best hit has pitfalls; results are far from perfect Manual annotation of individual proteins is not efficient ...
Essential Cell Biology Chapter 4 excerpt
... The a helix and the b Sheet are common folding patterns When the three-dimensional structures of many different protein molecules are compared, it becomes clear that, although the overall conformation of each protein is unique, two regular folding patterns are often present. Both were discovered mor ...
... The a helix and the b Sheet are common folding patterns When the three-dimensional structures of many different protein molecules are compared, it becomes clear that, although the overall conformation of each protein is unique, two regular folding patterns are often present. Both were discovered mor ...
IMPROVEMENT OF hGM-CSF EXPRESSION USING A FUSION
... system. However after sonication, the supernatant was measured for expressed protein and pMAL-p2 showed highest expression, followed by pMAL-c2 and i n pATH 11, pACT7 and p E T vector, n o expression was detected. Moreover in the pellet of these constructs highest level of expression was noticed in ...
... system. However after sonication, the supernatant was measured for expressed protein and pMAL-p2 showed highest expression, followed by pMAL-c2 and i n pATH 11, pACT7 and p E T vector, n o expression was detected. Moreover in the pellet of these constructs highest level of expression was noticed in ...
1. introduction - International Journal of Computer Applications
... Extracellular matrix (ECM) proteins are those secreted to the exterior of the cell, which act as mediators between resident cells and the external environment. These proteins not only maintain cellular structure but also play a part in diverse processes, including growth, hormonal response, homeosta ...
... Extracellular matrix (ECM) proteins are those secreted to the exterior of the cell, which act as mediators between resident cells and the external environment. These proteins not only maintain cellular structure but also play a part in diverse processes, including growth, hormonal response, homeosta ...
The Sec34/35 Golgi Transport Complex Is Related to the Exocyst
... McNew et al., 2000; Pelham, 2001). Although there are specific sets of SNARE proteins associated with each transport step, it is now clear that while they may contribute to the fidelity of fusion between membranes, other factors must also play a role. For several transport steps it has been possible ...
... McNew et al., 2000; Pelham, 2001). Although there are specific sets of SNARE proteins associated with each transport step, it is now clear that while they may contribute to the fidelity of fusion between membranes, other factors must also play a role. For several transport steps it has been possible ...
FT-IR Protein Structure Analyzer
... PROTA was introduced in 1998 as the first dedicated solution for structure elucidation of biologics and since has become the industry’s preferred choice. PROTA provides a fast, cost-effective and sensitive way to determine secondary structure of a protein or to follow structural changes due to pertu ...
... PROTA was introduced in 1998 as the first dedicated solution for structure elucidation of biologics and since has become the industry’s preferred choice. PROTA provides a fast, cost-effective and sensitive way to determine secondary structure of a protein or to follow structural changes due to pertu ...
University of Birmingham Armadillo
... cellular roles of both well-known and novel ARM-repeat proteins. Armadillo-repeat containing proteins Armadillo (ARM)-repeat proteins are characterised by containing a repeating 42 amino acid motif composed of three a-helices, which was first characterized in the Drosophila segment polarity protein ...
... cellular roles of both well-known and novel ARM-repeat proteins. Armadillo-repeat containing proteins Armadillo (ARM)-repeat proteins are characterised by containing a repeating 42 amino acid motif composed of three a-helices, which was first characterized in the Drosophila segment polarity protein ...
Impact of clostridial glucosylating toxins on the
... However, the studies from Gerhard et al. show a dependence on active TcdA leading to glucosylation of Rho GTPases for induction of apoptosis [11]. Thus, it is still unclear, how TcdA renders cells apoptotic. To further provide insights into these effects we investigated the changes in protein expres ...
... However, the studies from Gerhard et al. show a dependence on active TcdA leading to glucosylation of Rho GTPases for induction of apoptosis [11]. Thus, it is still unclear, how TcdA renders cells apoptotic. To further provide insights into these effects we investigated the changes in protein expres ...
English
... A. The stomach contains high concentrations of hydrochloric acid and the protein digestion enzyme called pepsin. Pepsin will only become active at low pH levels (around 2.0). Pepsin breaks down long globular proteins into shorter polypeptide chains. PowerPoint Slide 5. B. After the stomach, the prot ...
... A. The stomach contains high concentrations of hydrochloric acid and the protein digestion enzyme called pepsin. Pepsin will only become active at low pH levels (around 2.0). Pepsin breaks down long globular proteins into shorter polypeptide chains. PowerPoint Slide 5. B. After the stomach, the prot ...
Characterization of interactions between LPS transport proteins of
... By whichever method LptA functions, it is presumed that LPSbound LptA interacts with a component of the OM LptDE complex to transfer the LPS to the OM. LptE contains a lipid anchor attached to Cys19 but the N-terminus is not required for the interaction between LptD and LptE, and LptE appears to bin ...
... By whichever method LptA functions, it is presumed that LPSbound LptA interacts with a component of the OM LptDE complex to transfer the LPS to the OM. LptE contains a lipid anchor attached to Cys19 but the N-terminus is not required for the interaction between LptD and LptE, and LptE appears to bin ...
Expression and Purification of Toxoplasma gondii Cell Cycle
... Toxoplasma gondii is an Apicomplexan obligate intracellular protozoan parasite, which is able to infect virtually all warm-blooded animals. According to the Centers for Disease Control approximately 60 million people in the U.S. are currently infected with T. gondii. Treatments for toxoplasmosis are ...
... Toxoplasma gondii is an Apicomplexan obligate intracellular protozoan parasite, which is able to infect virtually all warm-blooded animals. According to the Centers for Disease Control approximately 60 million people in the U.S. are currently infected with T. gondii. Treatments for toxoplasmosis are ...
Membrane Proteins: Capturing the signal | eLife
... Capturing the signal High-resolution structures provide new insights into how an RNA-protein complex recognizes the signal that targets membrane proteins to the endoplasmic reticulum before they aggregate. ...
... Capturing the signal High-resolution structures provide new insights into how an RNA-protein complex recognizes the signal that targets membrane proteins to the endoplasmic reticulum before they aggregate. ...
Lipid modifications of proteins – slipping in and out of membranes
... can occur enzymatically and non-enzymatically, and the mechanisms that regulate each of these reactions are unknown. The most extensively studied examples of palmitoylated proteins are the a-subunits of trimeric GTP-binding proteins (Ga) in animal cells. Following association with an effector, Ga is ...
... can occur enzymatically and non-enzymatically, and the mechanisms that regulate each of these reactions are unknown. The most extensively studied examples of palmitoylated proteins are the a-subunits of trimeric GTP-binding proteins (Ga) in animal cells. Following association with an effector, Ga is ...
The Identity of Proteins Associated with a Small Heat Shock Protein
... of protein-folding diseases and aging (3, 4). The sHSPs are defined by a conserved C-terminal domain of ⬃90 amino acids (the ␣-crystallin domain), which is flanked by a variable length N-terminal arm and a more conserved C-terminal extension (1, 5). These small proteins (16 – 42-kDa monomers) assemb ...
... of protein-folding diseases and aging (3, 4). The sHSPs are defined by a conserved C-terminal domain of ⬃90 amino acids (the ␣-crystallin domain), which is flanked by a variable length N-terminal arm and a more conserved C-terminal extension (1, 5). These small proteins (16 – 42-kDa monomers) assemb ...
propy: a tool to generate various modes of
... functional, expression and interaction profiles of proteins and peptides. To facilitate extensive studies of proteins and peptides, we developed a freely available, open source python package called protein in python (propy) for calculating the widely used structural and physicochemical features of ...
... functional, expression and interaction profiles of proteins and peptides. To facilitate extensive studies of proteins and peptides, we developed a freely available, open source python package called protein in python (propy) for calculating the widely used structural and physicochemical features of ...
(pdf-file 1,2 Mb)
... The formation of eukaryotic ribosomes is a multistep process that takes place successively in the nucleolar, nucleoplasmic and cytoplasmic compartments1–4. Along this pathway, multiple preribosomal particles are generated, which transiently associate with numerous non-ribosomal factors before mature ...
... The formation of eukaryotic ribosomes is a multistep process that takes place successively in the nucleolar, nucleoplasmic and cytoplasmic compartments1–4. Along this pathway, multiple preribosomal particles are generated, which transiently associate with numerous non-ribosomal factors before mature ...
Protein import into the nucleus - Universitätsklinikum des Saarlandes
... of macromolecules between the cytoplasm and the nucleoplasm. This review will focus on new findings on protein import into the nucleus. Export of proteins and import/export of R N A have been reviewed recently [1-4]. Molecules of up to ,~ 50 kDa may pass the NPC by diffusion. However, even small pro ...
... of macromolecules between the cytoplasm and the nucleoplasm. This review will focus on new findings on protein import into the nucleus. Export of proteins and import/export of R N A have been reviewed recently [1-4]. Molecules of up to ,~ 50 kDa may pass the NPC by diffusion. However, even small pro ...
title page
... Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance --YJL218w Putative protein of unknown function, similar to bacterial galactoside Oacetyltransferases; ind ...
... Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance --YJL218w Putative protein of unknown function, similar to bacterial galactoside Oacetyltransferases; ind ...
Important Factors Influencing Protein Solubility for 2-D - Bio-Rad
... Denaturation and solubilization can require some time to complete. It might therefore be necessary to apply solubilization treatments for up to several hours before the sample is ready for IEF. Solubilization may be accelerated by sonicating the sample, but care must be taken that this treatment doe ...
... Denaturation and solubilization can require some time to complete. It might therefore be necessary to apply solubilization treatments for up to several hours before the sample is ready for IEF. Solubilization may be accelerated by sonicating the sample, but care must be taken that this treatment doe ...
coIP就是co-immunoprecipitation，假如有两种蛋白质A,B相互作用
... during the assay. Since transient interactions occur during transport or as part of enzymatic processes, they often require cofactors and energy via nucleotide triphosphates hydrolysis. Incorporating cofactors and nonhydrolyzable NTP analogs during assay optimization can serve to ‘trap’ interacting ...
... during the assay. Since transient interactions occur during transport or as part of enzymatic processes, they often require cofactors and energy via nucleotide triphosphates hydrolysis. Incorporating cofactors and nonhydrolyzable NTP analogs during assay optimization can serve to ‘trap’ interacting ...
Gene7-06
... majority of the mass is provided by rRNA. 2. Each subunit contains a single major rRNA, 16S and 23S in prokaryotes, 18S and 28S in eukaryotic cytosol. 3. Each subunit has several active centers, concentrated in the translational domain of the ribosome where proteins are synthesized. 4. The major rRN ...
... majority of the mass is provided by rRNA. 2. Each subunit contains a single major rRNA, 16S and 23S in prokaryotes, 18S and 28S in eukaryotic cytosol. 3. Each subunit has several active centers, concentrated in the translational domain of the ribosome where proteins are synthesized. 4. The major rRN ...
UBXD4, a UBX-Containing Protein, Regulates the Cell Surface
... nAChR cell surface expression, especially that of the ␣32 subtype. These findings were corroborated by electrophysiology, immunofluorescent staining, and biotinylation of surface receptors. Silencing of UBXD4 led to a significant reduction of ␣3* nAChRs in rat cortical neurons and dPC12 cells. Bioc ...
... nAChR cell surface expression, especially that of the ␣32 subtype. These findings were corroborated by electrophysiology, immunofluorescent staining, and biotinylation of surface receptors. Silencing of UBXD4 led to a significant reduction of ␣3* nAChRs in rat cortical neurons and dPC12 cells. Bioc ...
A REVIEW OF MICROBIAL PROTEIN PRODUCTION: PROSPECTS
... must be free from all kinds of pathogens, toxins, contaminants from heavy metals or other metal compounds, hydrocarbons and free from the risk of causing food allergies or cancer. It has been observed that most foreign proteins are not suitable for human consumption because of their ability to cause ...
... must be free from all kinds of pathogens, toxins, contaminants from heavy metals or other metal compounds, hydrocarbons and free from the risk of causing food allergies or cancer. It has been observed that most foreign proteins are not suitable for human consumption because of their ability to cause ...
Mechanisms of mitochondrial protein import
... residues; they usually lack acidic amino acids. Comparison of the primary structures of these signal sequences reveals no obvious homology or motif that might be responsible for the targeting function. These signal sequences, however, can adopt an amphiphilic -helix that might be important for thei ...
... residues; they usually lack acidic amino acids. Comparison of the primary structures of these signal sequences reveals no obvious homology or motif that might be responsible for the targeting function. These signal sequences, however, can adopt an amphiphilic -helix that might be important for thei ...
Enzymes
... • The substrate that the enzyme acts upon is able to diffuse through the gel, although this may be quite slow • Immobilised enzymes are widely used in industry because it allows the reaction to flow continuously and the product will not be contaminated with the enzyme so will not need to be purified ...
... • The substrate that the enzyme acts upon is able to diffuse through the gel, although this may be quite slow • Immobilised enzymes are widely used in industry because it allows the reaction to flow continuously and the product will not be contaminated with the enzyme so will not need to be purified ...
Proteasome
Proteasomes are protein complexes inside all eukaryotes and archaea, and in some bacteria. The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds.