![The Escherichia coli trmE (mnmE) gene, involved in tRNA](http://s1.studyres.com/store/data/022152689_1-06208ec8540d855312dc4d87889beba4-300x300.png)
The Escherichia coli trmE (mnmE) gene, involved in tRNA
... protein from strain MC1000/pIC684. The gel was stained with Coomassie Blue. Lane 1, crude extract from an uninduced culture; lane 2, crude extract from a culture induced for GST–50K overexpression with 0.5 mM IPTG for 3 h; lane 3, purified GST–50K; lane 4, protein remaining in the glutathione–Sephar ...
... protein from strain MC1000/pIC684. The gel was stained with Coomassie Blue. Lane 1, crude extract from an uninduced culture; lane 2, crude extract from a culture induced for GST–50K overexpression with 0.5 mM IPTG for 3 h; lane 3, purified GST–50K; lane 4, protein remaining in the glutathione–Sephar ...
The nullo protein is a component of the actin
... homology to known proteins, including the sry-α protein. Sequence analysis demonstrated that the nullo protein has an excess of basic amino acids (predicted pI is 11.4) and suggested that the protein may be myristoylated (Rose and Wieschaus, 1992). However, previous studies did not address intracell ...
... homology to known proteins, including the sry-α protein. Sequence analysis demonstrated that the nullo protein has an excess of basic amino acids (predicted pI is 11.4) and suggested that the protein may be myristoylated (Rose and Wieschaus, 1992). However, previous studies did not address intracell ...
A New Subfamily of Major Intrinsic Proteins in
... of the proteins. The third group of MIPs shows similarity with NOD26, a nodulin expressed in peribacteroid membrane surrounding the symbiotic nitrogen-fixing bacteria in nodules of soybean roots (Fortin, Morrison, and Verma 1987). These proteins are called NOD26-like MIPs (NLMs; Weig, Deswartes, and ...
... of the proteins. The third group of MIPs shows similarity with NOD26, a nodulin expressed in peribacteroid membrane surrounding the symbiotic nitrogen-fixing bacteria in nodules of soybean roots (Fortin, Morrison, and Verma 1987). These proteins are called NOD26-like MIPs (NLMs; Weig, Deswartes, and ...
Identification, Purification, and Molecular Cloning of
... added to isolated intact spinach chloroplasts, which subsequently were fractionated into a soluble fraction and fractions containing thylakoids and envelope membranes (Figure 7). The imported protein was completely absent from the soluble fraction (data not shown), and the processed mature protein ( ...
... added to isolated intact spinach chloroplasts, which subsequently were fractionated into a soluble fraction and fractions containing thylakoids and envelope membranes (Figure 7). The imported protein was completely absent from the soluble fraction (data not shown), and the processed mature protein ( ...
Protein expression by a Beijing strain differs from that of another
... W-like families responsible for many cases of drug resistance are a subset (Bifani et al., 2002). Molecular epidemiological studies have shown that these strains (both drug-susceptible and -resistant) are distributed worldwide and are able to spread in large clonal clusters (Glynn et al., 2002). The ...
... W-like families responsible for many cases of drug resistance are a subset (Bifani et al., 2002). Molecular epidemiological studies have shown that these strains (both drug-susceptible and -resistant) are distributed worldwide and are able to spread in large clonal clusters (Glynn et al., 2002). The ...
Green Fluorescent Protein
... • Cylinder has a diameter of about 30A and is about 40A long • Fluorophore located on central helix ...
... • Cylinder has a diameter of about 30A and is about 40A long • Fluorophore located on central helix ...
SOMAmer® anti-Carbonic anhydrase 3 Reagent
... Carbonic anhydrase XIII, Carbonic anhydrase VII, Carbonic anhydrase 6, Carbonic anhydrase X, or Carbonic anhydrase 9. Affinity The Kd for Carbonic anhydrase 3 binding to the SOMAmer reagent, measured ...
... Carbonic anhydrase XIII, Carbonic anhydrase VII, Carbonic anhydrase 6, Carbonic anhydrase X, or Carbonic anhydrase 9. Affinity The Kd for Carbonic anhydrase 3 binding to the SOMAmer reagent, measured ...
Intermediary Metabolism and Life-History Trade
... phospholipid was quantified as described previously (Zhao and Zera 2002). Amount of radioactivity incorporated into total protein was measured essentially as described by Geer and Downing (1972). The fat-free cricket residue was washed twice with 10% trichloroacetic acid, which removed unincorporate ...
... phospholipid was quantified as described previously (Zhao and Zera 2002). Amount of radioactivity incorporated into total protein was measured essentially as described by Geer and Downing (1972). The fat-free cricket residue was washed twice with 10% trichloroacetic acid, which removed unincorporate ...
MILK - Soegijapranata Catholic University
... About four fifths of the protein consists of casein, actually a mixture of four proteins: αS1-, αS2-, β-, and κ-casein. The caseins are typical for milk. The remainder consists, for the most part, of the milk serum proteins, the main one being βlactoglobulin. Moreover, milk contains numerous minor p ...
... About four fifths of the protein consists of casein, actually a mixture of four proteins: αS1-, αS2-, β-, and κ-casein. The caseins are typical for milk. The remainder consists, for the most part, of the milk serum proteins, the main one being βlactoglobulin. Moreover, milk contains numerous minor p ...
Hsp70 and Hsp90 of E. coli Directly Interact for Collaboration in
... of Hsp90. For example, Hop/Sti1 interacts simultaneously with Hsp70 and Hsp90 through its multiple tetratricopeptide repeat domains and facilitates substrate transfer from Hsp70 to Hsp90 by stabilizing the open conformation of Hsp90 [18,19]. The bacterial homolog of Hsp90 in Escherichia coli, the pr ...
... of Hsp90. For example, Hop/Sti1 interacts simultaneously with Hsp70 and Hsp90 through its multiple tetratricopeptide repeat domains and facilitates substrate transfer from Hsp70 to Hsp90 by stabilizing the open conformation of Hsp90 [18,19]. The bacterial homolog of Hsp90 in Escherichia coli, the pr ...
Amassin, an olfactomedin protein, mediates the massive
... probably carry oligosaccharide chains, since digestion with the enzyme peptide N-glycosidase-F (PNGase-F) decreases amassin’s Mr from 75 to 60 kD (Fig. 8 B), which is close to the calculated molecular weight of 56.5 kD. The remaining molecular weight discrepancy could be due to other posttranslation ...
... probably carry oligosaccharide chains, since digestion with the enzyme peptide N-glycosidase-F (PNGase-F) decreases amassin’s Mr from 75 to 60 kD (Fig. 8 B), which is close to the calculated molecular weight of 56.5 kD. The remaining molecular weight discrepancy could be due to other posttranslation ...
The DsbA Signal Sequence Directs Efficient
... this export. These findings indicate that SecA plays a role in cotranslational export. They also suggest that, via gene fusion analysis, thioredoxin can provide a sensitive assay system for signal sequences that direct proteins to the SRP pathway. MATERIALS AND METHODS Bacterial strains and growth c ...
... this export. These findings indicate that SecA plays a role in cotranslational export. They also suggest that, via gene fusion analysis, thioredoxin can provide a sensitive assay system for signal sequences that direct proteins to the SRP pathway. MATERIALS AND METHODS Bacterial strains and growth c ...
nmr.evilia.190303
... In structure work, J coupling is critical to measuring the bond angles and dihedral angles between nuclei. The Karplus equation is used to calculate the dihedral angles for 3 bond or vicinal coupling: J = A + B cosq+ C cos2q Where A, B, C are coefficients that depend on the nuclei ...
... In structure work, J coupling is critical to measuring the bond angles and dihedral angles between nuclei. The Karplus equation is used to calculate the dihedral angles for 3 bond or vicinal coupling: J = A + B cosq+ C cos2q Where A, B, C are coefficients that depend on the nuclei ...
Protein S-nitrosylation in photosynthetic organisms: A
... 2.1. Major systems of NO production In animal systems the production of nitric oxide (NO) has been extensively characterized over the last decades. Nowadays it is universally accepted that the main pathway goes through nitric oxide synthase (NOS), a ubiquitous enzyme existing in three isoforms: neur ...
... 2.1. Major systems of NO production In animal systems the production of nitric oxide (NO) has been extensively characterized over the last decades. Nowadays it is universally accepted that the main pathway goes through nitric oxide synthase (NOS), a ubiquitous enzyme existing in three isoforms: neur ...
Chapter 14 Preparing Semisynthetic and Fully Synthetic Histones
... Chemical ligation is an excellent way to prepare the homogenous samples of precisely modified histone proteins that are necessary to characterize the molecular functions of these modifications within the structured nucleosome core (5). Native chemical ligation (NCL) is the chemoselective condensatio ...
... Chemical ligation is an excellent way to prepare the homogenous samples of precisely modified histone proteins that are necessary to characterize the molecular functions of these modifications within the structured nucleosome core (5). Native chemical ligation (NCL) is the chemoselective condensatio ...
The AMP-activated protein kinase pathway – new
... nucleotides, ATP and ADP, and the chemicals in an electrical cell or battery. Living cells normally maintain a high ratio of ATP to ADP (typically around 10:1), which is many orders of magnitude away from the equilibrium ratio for ATP hydrolysis under cellular conditions. This represents a store of ...
... nucleotides, ATP and ADP, and the chemicals in an electrical cell or battery. Living cells normally maintain a high ratio of ATP to ADP (typically around 10:1), which is many orders of magnitude away from the equilibrium ratio for ATP hydrolysis under cellular conditions. This represents a store of ...
Resistance Gene-Dependent Activation of a Calcium
... kinase (Song et al., 1995). In addition, in vivo phosphorylation experiments and studies with pharmacological inhibitors have demonstrated that protein kinases and phosphatases are crucial for activation of early defense responses (Yang et al., 1997; Scheel, 1998). Recent reports have noted the acti ...
... kinase (Song et al., 1995). In addition, in vivo phosphorylation experiments and studies with pharmacological inhibitors have demonstrated that protein kinases and phosphatases are crucial for activation of early defense responses (Yang et al., 1997; Scheel, 1998). Recent reports have noted the acti ...
THE RDOA-DEPENDENT PHOSPHOPROTEOME PROFILE OF Salmonella enterica
... Phosphorylation is a widely studied post-translational protein modification that can be observed in both prokaryotic and eukaryotic systems. This process is controlled by two types of enzymes, kinases and phosphatases, that enable the transfer of phosphate from a nucleotide triphosphates such as ade ...
... Phosphorylation is a widely studied post-translational protein modification that can be observed in both prokaryotic and eukaryotic systems. This process is controlled by two types of enzymes, kinases and phosphatases, that enable the transfer of phosphate from a nucleotide triphosphates such as ade ...
Charge asymmetry in the proteins of the outer membrane
... outer membrane phospholipase in lipid bilayer vesicles demonstrated that substitution of leucine for native residues in lipidfacing positions stabilizes OMBBs and substitution of those same positions to arginine destabilizes OMBBs. The effect of these mutations on stabilization is stronger toward th ...
... outer membrane phospholipase in lipid bilayer vesicles demonstrated that substitution of leucine for native residues in lipidfacing positions stabilizes OMBBs and substitution of those same positions to arginine destabilizes OMBBs. The effect of these mutations on stabilization is stronger toward th ...
Effects of phosphatidylethanolamine glycation on lipid–protein
... monolayer is intimately related to the stability of membrane proteins [16]. This result suggests that alterations in the monolayer composition are translated into modifications on the structure of membrane proteins. In this context, a chemical modification of either lipids or proteins, such as those ...
... monolayer is intimately related to the stability of membrane proteins [16]. This result suggests that alterations in the monolayer composition are translated into modifications on the structure of membrane proteins. In this context, a chemical modification of either lipids or proteins, such as those ...
Regulation Of Glut4 Biogenesis In Muscle Evidence For
... administration of 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) to rats, or exposing muscles to AICAR in vitro, results in a rapid increase in GLUT4 protein (18, 30, 41, 43). AICAR is taken up by muscle cells and converted to the AMP analog ZMP, which activates AMPK (40). Endurance exercise ...
... administration of 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) to rats, or exposing muscles to AICAR in vitro, results in a rapid increase in GLUT4 protein (18, 30, 41, 43). AICAR is taken up by muscle cells and converted to the AMP analog ZMP, which activates AMPK (40). Endurance exercise ...
Membrane Topology of Cytochrome P450 2B4 in
... 60 s. A similar rapid pressure increase was seen when b5 was injected beneath DPPC monolayers. The pressure increased by as much as 1.5 mN in the first 20 s after injection at high subphase concentrations (Fig. 1). The pressure continued to rise for the next several hours, but at a much slower rate ...
... 60 s. A similar rapid pressure increase was seen when b5 was injected beneath DPPC monolayers. The pressure increased by as much as 1.5 mN in the first 20 s after injection at high subphase concentrations (Fig. 1). The pressure continued to rise for the next several hours, but at a much slower rate ...
Protein (nutrient)
Proteins are essential nutrients for the human body. They are one of the building blocks of body tissue, and can also serve as a fuel source. As a fuel, proteins contain 4 kcal per gram, just like carbohydrates and unlike lipids, which contain 9 kcal per gram. The most important aspect and defining characteristic of protein from a nutritional standpoint is its amino acid composition.Proteins are polymer chains made of amino acids linked together by peptide bonds. During human digestion, proteins are broken down in the stomach to smaller polypeptide chains via hydrochloric acid and protease actions. This is crucial for the synthesis of the essential amino acids that cannot be biosynthesized by the body.There are nine essential amino acids which humans must obtain from their diet in order to prevent protein-energy malnutrition. They are phenylalanine, valine, threonine, tryptophan, methionine, leucine, isoleucine, lysine, and histidine. There are five dispensable amino acids which humans are able to synthesize in the body. These five are alanine, aspartic acid, asparagine, glutamic acid and serine. There are six conditionally essential amino acids whose synthesis can be limited under special pathophysiological conditions, such as prematurity in the infant or individuals in severe catabolic distress. These six are arginine, cysteine, glycine, glutamine, proline and tyrosine.Humans need the essential amino acids in certain ratios. Some protein sources contain amino acids in a more or less 'complete' sense. This has given rise to various ranking systems for protein sources, as described in the article.Animal sources of protein include meats, dairy products, fish and eggs. Vegan sources of protein include whole grains, pulses, legumes, soy, and nuts. Vegetarians and vegans can get enough essential amino acids by eating a variety of plant proteins. It is commonly believed that athletes should consume a higher-than-normal protein intake to maintain optimal physical performance.