The Code of Life: Topic 3
... • Gene expression! • You have 23 pairs of chromosomes. • In each pair you get one from your mother and one from your father. • Each chromosome in a pair holds all the same genes as the other. • So what determines which gene is expressed when you develop? • ie how do you get your mother's eyes or you ...
... • Gene expression! • You have 23 pairs of chromosomes. • In each pair you get one from your mother and one from your father. • Each chromosome in a pair holds all the same genes as the other. • So what determines which gene is expressed when you develop? • ie how do you get your mother's eyes or you ...
RECOMBINANT DNA
... 12. Scientists make healthier pork by genetically modifying a pig with spinach genes. Meat and vegetable at the same time. ...
... 12. Scientists make healthier pork by genetically modifying a pig with spinach genes. Meat and vegetable at the same time. ...
042310_recombinant_DNA2
... copies could be generated) • A recognition sequence for a restriction enzyme (so that we can introduce our DNA of interest) • Reporter genes (to confirm we have successfully introduced the vector into the host cell) • Small size in comparison with host’s chromosomes (for easy manipulation) ...
... copies could be generated) • A recognition sequence for a restriction enzyme (so that we can introduce our DNA of interest) • Reporter genes (to confirm we have successfully introduced the vector into the host cell) • Small size in comparison with host’s chromosomes (for easy manipulation) ...
DNA Technology
... • Carries many genes • Genes occupy only a small portion of the chromosome • A specific gene probably only makes up 1/1000,000 of the DNA chromosomal molecule • Subtle differences distinguish the gene from the surrounding material ...
... • Carries many genes • Genes occupy only a small portion of the chromosome • A specific gene probably only makes up 1/1000,000 of the DNA chromosomal molecule • Subtle differences distinguish the gene from the surrounding material ...
Biotechnology
... VNTRs can occur only once in the genome (single locus) or can occur in a number of places in the genome (multilocus). Single locus probes are fine for paternity cases (each individual has two VNTR ...
... VNTRs can occur only once in the genome (single locus) or can occur in a number of places in the genome (multilocus). Single locus probes are fine for paternity cases (each individual has two VNTR ...
GENETIC ENGINEERING QUESTIONS
... 5. In a cloning experiment, the plasmid used to insert the gene of interest into has an antibiotic resistant gene in it. When bacteria mixed with plasmid are grown in the present of antibiotic a. Only bacteria without the plasmid will grow b. Only bacteria with the plasmid will grow c. All bacteria ...
... 5. In a cloning experiment, the plasmid used to insert the gene of interest into has an antibiotic resistant gene in it. When bacteria mixed with plasmid are grown in the present of antibiotic a. Only bacteria without the plasmid will grow b. Only bacteria with the plasmid will grow c. All bacteria ...
CH 16 PPT
... Mendel: modes of heredity in pea plants Morgan: genes located on chromosomes Griffith: bacterial work; transformation: change in genotype and phenotype due to assimilation of external substance (DNA) by a cell Avery: transformation agent was DNA ...
... Mendel: modes of heredity in pea plants Morgan: genes located on chromosomes Griffith: bacterial work; transformation: change in genotype and phenotype due to assimilation of external substance (DNA) by a cell Avery: transformation agent was DNA ...
Vocabulary DNA Structure
... the pairing of complementary strands of DNA through hydrogen bonding ...
... the pairing of complementary strands of DNA through hydrogen bonding ...
Geneticsworksheet
... 12. Why do scientists use computer programs to model protein structure and function? ______________________________________________________________________________ ______________________________________________________________________________ 13. What provides the “blueprint” for making a protein? _ ...
... 12. Why do scientists use computer programs to model protein structure and function? ______________________________________________________________________________ ______________________________________________________________________________ 13. What provides the “blueprint” for making a protein? _ ...
Protein Synthesis Review
... 4. Name three types of RNA (one is from DNA replication, two from protein synthesis) described and explain the function of each. 5. How many different DNA triplets are possible? 6. The DNA triplet “CGA” is transcribed into which RNA codon? a) GUT (b) GUC (c) GCU (d) AUG 7. Which enzyme “reads” the m ...
... 4. Name three types of RNA (one is from DNA replication, two from protein synthesis) described and explain the function of each. 5. How many different DNA triplets are possible? 6. The DNA triplet “CGA” is transcribed into which RNA codon? a) GUT (b) GUC (c) GCU (d) AUG 7. Which enzyme “reads” the m ...
Transcription
... • Enzymes attach to DNA at the gene’s location and unzip only where that gene is on the DNA. – DNA A T C G ...
... • Enzymes attach to DNA at the gene’s location and unzip only where that gene is on the DNA. – DNA A T C G ...
CHEM523 Test 3
... iii) Draw the reaction mechanism for the enzyme. If you need help, please raise your hand and I will “sell” you the relevant species involved in the mechanism for 2 points. It will be up to you to arrange them into a mechanism. ...
... iii) Draw the reaction mechanism for the enzyme. If you need help, please raise your hand and I will “sell” you the relevant species involved in the mechanism for 2 points. It will be up to you to arrange them into a mechanism. ...
Answer Key DNA Review - John Bowne High School
... 24. Researchers have found that formaldehyde and asbestos can alter DNA base sequences. Based on this research, the use of these chemicals has been greatly reduced because they A) may act as fertilizers, increasing the growth of algae in ponds B) have been replaced by more toxie compounds C) are cap ...
... 24. Researchers have found that formaldehyde and asbestos can alter DNA base sequences. Based on this research, the use of these chemicals has been greatly reduced because they A) may act as fertilizers, increasing the growth of algae in ponds B) have been replaced by more toxie compounds C) are cap ...
Does your DNA define you Qu
... are around genes encoded in the genome so if each cell in the body has the same DNA molecules so what makes a skin cell a skin cell and not a liver cell? The development of an organism and the subsequent specialisation of each cell of the human body is controlled by sets of chemical reactions that s ...
... are around genes encoded in the genome so if each cell in the body has the same DNA molecules so what makes a skin cell a skin cell and not a liver cell? The development of an organism and the subsequent specialisation of each cell of the human body is controlled by sets of chemical reactions that s ...
LECTURE 16 – Using Genomic Variation for Identity DNA Level
... Ø Restriction enzymes cut the DNA leaving a sticky end (overhang of one DNA strand) or a blunt end (strands cut at same point) Ø Restriction enzymes will only cut certain sequences of bases in the DNA ...
... Ø Restriction enzymes cut the DNA leaving a sticky end (overhang of one DNA strand) or a blunt end (strands cut at same point) Ø Restriction enzymes will only cut certain sequences of bases in the DNA ...
Les 1-DNA Structure-review
... Each unique gene has a unique sequence of bases. This unique sequence of bases will code for the ...
... Each unique gene has a unique sequence of bases. This unique sequence of bases will code for the ...
DNA isol
... 3. If you had to choose, be gentle rather that be rough. Doesn’t hurt to be careful when handling material. i.e. keep everything cold, since these enzymes are much more active at physiological temperatures. (i.e. use of "ice cold" this and that") Wear gloves, etc. 4. Know the idiosyncrasies of your ...
... 3. If you had to choose, be gentle rather that be rough. Doesn’t hurt to be careful when handling material. i.e. keep everything cold, since these enzymes are much more active at physiological temperatures. (i.e. use of "ice cold" this and that") Wear gloves, etc. 4. Know the idiosyncrasies of your ...
Restriction Enzymes - Seattle Central College
... • Restriction enzymes are bacterial enzymes that cleave the sugar-phosphate backbone of the DNA at specific nucleotide. • They are member of the class of nucleases. Endonucleases cleave nucleic acid at internal positions, while exonucleases progressively digest from the ends of the nucleic acid mole ...
... • Restriction enzymes are bacterial enzymes that cleave the sugar-phosphate backbone of the DNA at specific nucleotide. • They are member of the class of nucleases. Endonucleases cleave nucleic acid at internal positions, while exonucleases progressively digest from the ends of the nucleic acid mole ...
MOLECULAR BIOLOGY.rtf
... bases) --1 sugar = deoxyribose -- 4 bases = adenine, guanine, cytosine, and thymine -- has 5’ and 3’ ends -- double stranded in an antiparallel fashion (5’end of 1 strand bases pairs w/ 3’ end of the other strand) -- stabilized by covalent bonds between sugar and phosphate and between bases and suga ...
... bases) --1 sugar = deoxyribose -- 4 bases = adenine, guanine, cytosine, and thymine -- has 5’ and 3’ ends -- double stranded in an antiparallel fashion (5’end of 1 strand bases pairs w/ 3’ end of the other strand) -- stabilized by covalent bonds between sugar and phosphate and between bases and suga ...
Reproduction and Heredity
... Two nuclear divisions in meiosis, only one in mitosis Four haploid cells result from meiosis, Two diploid cells result from mitosis Nuclei produced by mitosis have identical gene combinations ...
... Two nuclear divisions in meiosis, only one in mitosis Four haploid cells result from meiosis, Two diploid cells result from mitosis Nuclei produced by mitosis have identical gene combinations ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.