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Lecture 1 Genetics – An Overview Professor Jane Farrar School of
Lecture 1 Genetics – An Overview Professor Jane Farrar School of

... The same 3 billion base pairs of DNA are present in every cell of your body. Approximately 25,000 genes present in 23 pairs of human chromosomes in the 3 billion DNBA base pairs How does each cell function so differently? Not all genes are active in all cell types. Genes make RNA which is translate ...
microbio 40 [4-20
microbio 40 [4-20

...  Aids transmissibility and makes them resistant to detergents, dessication, and heat  usually by direct skin to skin contact, and even then it requires a skin break  can also be spread by exfoliation onto inanimate objects 3. What is the result when HPV is transferred to a child during birth? Wha ...
NOTES: 12.2 – 12.3 – DNA Structure
NOTES: 12.2 – 12.3 – DNA Structure

... -# of chromosomes varies widely from species to species DNA molecules are long…how does DNA fit in the nucleus? ● It forms ...
Lesson Plan
Lesson Plan

... significance of these changes. ...
Document
Document

... 13. Several forms of RNA or ______________________ help change DNA code into proteins. 14. Because it is so similar to ______________________, RNA can serve as a temporary copy of a DNA sequence. 15. The “factory” that assembles proteins is known as a(n) ______________________. 16. A mirror-like cop ...
Life Science Vocabulary.xlsx
Life Science Vocabulary.xlsx

... an organism that always produces an offspring with the same form of a trait as the purebred parent trait a characteristic that an organism can pass on to its offspring through its genes allele the different forms of a gene Rosalind Franklin famous woman scientist who used x-rays to photograph DNA mo ...
Word Definition Synonym 1 DNA replication the
Word Definition Synonym 1 DNA replication the

... an organism that always produces an offspring with the same form of a trait as the purebred parent trait a characteristic that an organism can pass on to its offspring through its genes allele the different forms of a gene Rosalind Franklin famous woman scientist who used x-rays to photograph DNA mo ...
DIR RD 4C-2
DIR RD 4C-2

... 13. Several forms of RNA or ______________________ help change DNA code into proteins. 14. Because it is so similar to ______________________, RNA can serve as a temporary copy of a DNA sequence. 15. The “factory” that assembles proteins is known as a(n) ______________________. 16. A mirror-like cop ...
DNA ppt
DNA ppt

... 1. Enzyme (helicase) unzips the DNA. 2. DNA polymerase (enzyme) matches the correct nucleotide to each side of the unzipped DNA. 3. DNA polymerase – proofreads their replication to make sure they did not make any mistakes. ...
Multiple choice questions
Multiple choice questions

... require a primer to function require nucleotides to function require ATP ...
PRE-AP Stage 3 – Learning Plan
PRE-AP Stage 3 – Learning Plan

... SCAFFOLD: Students will identify the components of DNA and describe how genetic information is carried in DNA. After identifying the components of the structure of DNA, students will explain how DNA is transcribed and translated into amino acids to make proteins. ACCELERATE: PREAP – purines, pyrimid ...
Strawberry DNA Extraction
Strawberry DNA Extraction

... 4. Pour a small amount (three to four milliliters) of the filtered strawberry solution into a test tube. Tilt the tube and pour an equal amount of cold isopropyl alcohol into the test tube. The DNA will precipitate to the top of the solution and will resemble a white, fluffly cloud. What's Happening ...
DNA, RNA, and GENES
DNA, RNA, and GENES

... • Ribosomal RNA makes up ribosomes, where proteins are built. • Transfer RNA brings amino acids to the ribosomes to build the proteins. ...
After Gel Electrophoresis…
After Gel Electrophoresis…

... Share with your neighbor one thing that you would like to use these new genetic techniques for  My example - figuring out where I got my red hair from; or combining my two ...
Detecting and Modeling Long Range Correlation in Genomic
Detecting and Modeling Long Range Correlation in Genomic

... A genome encodes information that is needed to create complex machineries combining DNA, RNA and proteins. However, this structure has evolved by certain basic biological processes that modify the genome in a specific but stochastic manner, and has been shaped by selection pressure. With complete se ...
Biology 325: Genetics
Biology 325: Genetics

... Prokaryotic Gene Regulation: To enable bacteria to respond to their environments, transcription initiation is turned on and off mainly by trans-acting proteins; gene expression is also regulated after initiation by cis- or transacting RNAs, or trans-acting proteins. Eukaryotic Gene Regulation: Multi ...
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Test review Warm-up

... Which component of DNA provides instructions for the production of the ...
Review for Post Exam 10 on iLearn
Review for Post Exam 10 on iLearn

DNA and RNA Review
DNA and RNA Review

... Define: transcription, translation, replication, and transformation (**BE SURE to specify and describe what happens in each process**) ...
Topic 4 Genetics
Topic 4 Genetics

... Other chromosome level mutations are translocations and crossing over. Pieces of the chromosome break off and re-attach elsewhere, or exchange with homologous alleles. Mutations can occur at the base-pair level. Point mutations involve a single change in a nucleotide base. This may or may not result ...
Document
Document

... An expression vector (i.e. plasmid) is engineered to contain regulatory sequences regions (e.g. promoter), an origin of replication, a selectable marker, and a suitable site for the insertion of a gene of interest such as the multiple cloning site. ...
Expanded Genetic Code in a Bacterium
Expanded Genetic Code in a Bacterium

... Expanding the Genetic Code • At the Scripps Institute in California, scientists have engineered a bacterium with an expanded genetic code. • In addition to A, T, G, and C, they have added to synthetic nucleotides: d5SICS and dNaM (known as Y and X for short). ...
Bacteriophages
Bacteriophages

... ss-circular DNA (size 6407 bp) DNA enter in to cell converted to double stranded molecule known as replicative form or RF. Replicates until there are about 100 copies in the cell. ...
Ch. 12 Review- pg. 315 1-23 Answers The process by which one
Ch. 12 Review- pg. 315 1-23 Answers The process by which one

... Name two major types of mutations. Why do they have in common? Who are they different? Give an example for each. Gene and chromosomal; both change the DNA sequence that affects genetic information. Gene mutations involve a change in one or several nucleotides in a single gene, whereas chromosomal mu ...
Test - Easy Peasy All-in
Test - Easy Peasy All-in

... 1. How many genes do humans have? a. 2,500 b. 25,000 c. 20,000 ...
< 1 ... 718 719 720 721 722 723 724 725 726 ... 766 >

Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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