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Biology Final Jeopary 1
Biology Final Jeopary 1

... Q: What is one half (50%)? ...
Name
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... A. The entire gene is deleted. B. The entire gene is duplicated. C. Three bases are deleted, causing one amino acid to be missing. D. Three bases are duplicated, causing one amino acid show up about 40 times. 17. Why are individuals who are heterozygous for the cystic fibrosis allele unaffected by t ...
DNA Notes
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... phosphorus-32 in its DNA & was used to infect a bacterial cell • The other had radioactive sulfur-35 in its protein coat & was used to infect a bacterial cell • Both bacterial cells were examined • Only the bacteriophage with P-32 was inside the bacterial cell, proving that DNA is the material that ...
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W09micr430Lec17 - Cal State LA

... A common type of DNA damage is the deamination of bases (amino group is replaced by keto group) Deaminated bases pair with wrong bases during replication, creating mutations To repair, deaminated bases are removed by DNA glycosylases – catalyzing breakage of the N-glycosyl bond between the base and ...
A Comparison of Concentration Methods for Low Copy Number
A Comparison of Concentration Methods for Low Copy Number

... A commonly encountered hurdle in the processing of forensic DNA samples is the amount of DNA available for STR genotyping. This can be a function of either the sample itself, the collection of DNA from the sample, or the DNA extraction process. Standard low copy number (LCN) DNA typing techniques ar ...
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GENETIC ENGINEERING

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Viral Lytic and Lysogenic Cycles
Viral Lytic and Lysogenic Cycles

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GENETIC ENGINEERING

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... genes; and introns do not interrupt the cloned sequence. Disadvantages: contain only sequences that are presence in mature mRNA; and sequences expressed in the tissue from which RNA was isolated. ...
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Recombinant DNA Technology
Recombinant DNA Technology

... • Genetic engineering, engineering recombinant DNA technology, genetic modification/manipulation (GM) and gene splicing are terms that are applied to the direct manipulation of an organisms genes. • Recombinant DNA is a form of artificial DNA which is engineered through the combination or insertion ...
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... organism that is used to create a new identical copy of that same organism as an offspring ...
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4.4 Genetic Engineering and Biotechnology

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... Substitution: one base for another CACCTTATTA Deletion: missing a base CACCG ATTA Addition: adding a base CACCGTAATTA Inversion: bases are rearranged CACCTAGTTA ...
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Topic 4.4 - Genetic Engineering and Biotechnology

... enzyme recognises unique sequences of DNA in the plasmid and in the target DNA. It will cut DNA, producing “sticky ends”. Complementary sticky ends in target DNA and the plasmid allow incorporation of the target DNA into the plasmid, producing recombinant DNA. DNA ligase creates covalent bonds joini ...
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Discovery of DNA structure

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ModernGeneticsII
ModernGeneticsII

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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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