DNA Extraction Lab
... Extraction Solutions: 1. Bacterial Suspension Medium – used to make a solution of the cells 2. Lysozyme – enzyme that hydrolyses specific bonds that hold the cell wall 3. SDS – a detergent that lyses the cell by removing the lipids from the cell membrane By adding these solutions to a test tube of ...
... Extraction Solutions: 1. Bacterial Suspension Medium – used to make a solution of the cells 2. Lysozyme – enzyme that hydrolyses specific bonds that hold the cell wall 3. SDS – a detergent that lyses the cell by removing the lipids from the cell membrane By adding these solutions to a test tube of ...
Molecular genetics of bacteria
... Genetic regulation • Genotype is not phenotype: bacteria possess many genes that they are not using at any particular time. • Transcription and translation are expensive; why spend ATP to make an enzyme you don’t need? • Examples: – Induction of lactose operon – diauxic growth with sugars. ...
... Genetic regulation • Genotype is not phenotype: bacteria possess many genes that they are not using at any particular time. • Transcription and translation are expensive; why spend ATP to make an enzyme you don’t need? • Examples: – Induction of lactose operon – diauxic growth with sugars. ...
05E-NucleicAcids
... • Adenine (A) always pairs with thymine (T) and guanine (G) with cytosine (C). ...
... • Adenine (A) always pairs with thymine (T) and guanine (G) with cytosine (C). ...
last of Chapter 11, all of Chapter 12
... (insects, amphibians, and fish) increase in number. – (600 copies tandemly duplicated in normal toad genome, but more are needed: 4000-fold increase in gene copy number via rolling circle replicating extrachromosomal rRNA genes, over 3 weeks during oogenesis). ...
... (insects, amphibians, and fish) increase in number. – (600 copies tandemly duplicated in normal toad genome, but more are needed: 4000-fold increase in gene copy number via rolling circle replicating extrachromosomal rRNA genes, over 3 weeks during oogenesis). ...
Genetics Review
... Genes control the layout, make-up and function of the bodies of all organisms. Examples of traits influenced by genes: • Appearance (hair, skin, eyes, height, etc.) • Body structure of an organism • Susceptibility to diseases • Personality traits • Behavior (instincts as well as other behaviors) ...
... Genes control the layout, make-up and function of the bodies of all organisms. Examples of traits influenced by genes: • Appearance (hair, skin, eyes, height, etc.) • Body structure of an organism • Susceptibility to diseases • Personality traits • Behavior (instincts as well as other behaviors) ...
DNA Notes Day 2 PowerPoint
... • DNA is double stranded – base pairing allows for easy copying; one strand serves as a template for a new strand • Replication – the process of making a new DNA strand • DNA double helix is unwound by an enzyme called a helicase. Helicase breaks hydrogen bonds linking the nitrogen bases ...
... • DNA is double stranded – base pairing allows for easy copying; one strand serves as a template for a new strand • Replication – the process of making a new DNA strand • DNA double helix is unwound by an enzyme called a helicase. Helicase breaks hydrogen bonds linking the nitrogen bases ...
Ei dian otsikkoa
... DNA repair enzymes. The transferred DNA is thus, either degraded or used as a substrate for DNA repair, resulting in its potential rearrangement and incorporation in the genomic DNA (Takano et al. (1997) Plant J 11: 353-361 ). Furthermore, specific transforming plasmid structure and construct proper ...
... DNA repair enzymes. The transferred DNA is thus, either degraded or used as a substrate for DNA repair, resulting in its potential rearrangement and incorporation in the genomic DNA (Takano et al. (1997) Plant J 11: 353-361 ). Furthermore, specific transforming plasmid structure and construct proper ...
Modification of Genes and Proteins - sharonap-cellrepro-p2
... Do not press any buttons during the presentation, it will progress on its own. ...
... Do not press any buttons during the presentation, it will progress on its own. ...
Exam 3 4/25/07 BISC 4A P. Sengupta Total of 7 questions, 100
... 10. Primers in PCR reactions: A) Hybridize to only one DNA strand B) Elongate the DNA strands C) Hybridize to both DNA strands and make complementary copies ...
... 10. Primers in PCR reactions: A) Hybridize to only one DNA strand B) Elongate the DNA strands C) Hybridize to both DNA strands and make complementary copies ...
FA15Lec8 Sequencing DNA and RNA
... Figure 6-27 The sex-determination genes from humans and whales are unmistakably similar. Although their body plans are strikingly different, humans and whales are built from the same proteins. Despite the length of time since humans and whales diverged, the nucleotide sequences of many of their gene ...
... Figure 6-27 The sex-determination genes from humans and whales are unmistakably similar. Although their body plans are strikingly different, humans and whales are built from the same proteins. Despite the length of time since humans and whales diverged, the nucleotide sequences of many of their gene ...
molecular genetics
... DNA of phage takes over cell mechanics Cell makes components (proteins and nucleic acids) of the virus Cell assembles components into new viruses New viruses burst out of the cell, resulting in host cell’s death New viruses can then infect other cells. ...
... DNA of phage takes over cell mechanics Cell makes components (proteins and nucleic acids) of the virus Cell assembles components into new viruses New viruses burst out of the cell, resulting in host cell’s death New viruses can then infect other cells. ...
ppt
... B. No, the exons will need to be cut out and the introns spliced back together. C. No, the introns will need to be cut out and the exons spliced back together. D. No, the exons will need to be cut out, the introns translated individually, and the peptides bound together after translation. ...
... B. No, the exons will need to be cut out and the introns spliced back together. C. No, the introns will need to be cut out and the exons spliced back together. D. No, the exons will need to be cut out, the introns translated individually, and the peptides bound together after translation. ...
Lesson 2
... The genetic code is a set of “rules” by which the information in mRNA is translated into proteins. A codon is made of three nucleotide bases (triplet) and it may translate into: • an amino acid, • a “start” signal (which is always the amino acid Met), or • a “stop” signal (no amino acid) http://www ...
... The genetic code is a set of “rules” by which the information in mRNA is translated into proteins. A codon is made of three nucleotide bases (triplet) and it may translate into: • an amino acid, • a “start” signal (which is always the amino acid Met), or • a “stop” signal (no amino acid) http://www ...
Genetics EOC Review
... ________________ controlling a trait. Example = __________________. Polygenic inheritance is when you have two or more __________________ controlling a single trait. Example = _______________________. ...
... ________________ controlling a trait. Example = __________________. Polygenic inheritance is when you have two or more __________________ controlling a single trait. Example = _______________________. ...
DNA Analysis of Various Mouse Organs
... concentrations between organs. • Gel electrophoresis allowed for visualization of DNA from the varying organ tissues. ...
... concentrations between organs. • Gel electrophoresis allowed for visualization of DNA from the varying organ tissues. ...
stucture of DNA
... The discovery that genetic information is coded along the length of a polymeric molecules composed of only four types of monomeric units was one of the major scientific achievements of this century. This polymeric molecules, DNA, is the chemical basis of heredity and is organized into genes, the ...
... The discovery that genetic information is coded along the length of a polymeric molecules composed of only four types of monomeric units was one of the major scientific achievements of this century. This polymeric molecules, DNA, is the chemical basis of heredity and is organized into genes, the ...
Polymerase Chain Reaction and DNA Sequencing
... reactions can be radioactively labeled. • Bands detected by Xray film exposure. • Sequence can be read in the 5’ to 3’ direction from the bottom of the image towards the top. A A T C T A A C G ...
... reactions can be radioactively labeled. • Bands detected by Xray film exposure. • Sequence can be read in the 5’ to 3’ direction from the bottom of the image towards the top. A A T C T A A C G ...
... Maintain diploid number of chromosomes 2n 2n Exact copy of Genetic material in daughter cells. Cell cycle: G1 – cell growth S – replication of DNA, chromosomes are duplicated. G2 – Prior to division M – mitosis, separation of chromosomes. Interphase = G1-S-G2 Cell cycle regulated by cyclins ...
dna methylation
... Calorie consumption dropped from 2,000 to 500 per day for 4.5 million. Children born or raised in this time were small, short in stature and had many diseases including, edema, anemia, diabetes and depression. The Dutch Famine Birth Cohort study showed that women living during this time had children ...
... Calorie consumption dropped from 2,000 to 500 per day for 4.5 million. Children born or raised in this time were small, short in stature and had many diseases including, edema, anemia, diabetes and depression. The Dutch Famine Birth Cohort study showed that women living during this time had children ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.