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Genetics 314 – Spring 2005
Genetics 314 – Spring 2005

... can occur. Both viruses must be within the same cell for an exchange to occur. b) Describe one method of recombination that could lead to a more virulent avian flu virus. Once both viruses are within the same cell recombination can occur through several mechanisms. The first would be a simple error ...
Powerpoint Presentation: Gene Transfer
Powerpoint Presentation: Gene Transfer

...  Every time the bacterium divides the plasmid is replicated too  Gene expressed by the bacterium  Same protein is synthesised  Universal genetic code  Human proteins can be produced by bacteria  E.g. Humulin (Human Insulin) E.g. Human somatotropin (growth ...
No Slide Title
No Slide Title

... Bacteria have > 1 protein/mRNA (polycistronic) • Mutations can have polar effects: mutations in upstream genes may affect expression of perfectly good downstream genes! ...
Biology 303 EXAM III
Biology 303 EXAM III

... 3. LOH via gene targeting. 4. the discovery of a specific translocation associated with chronic myelogenous leukemia ...
MUTATION, DNA REPAIR AND CANCER
MUTATION, DNA REPAIR AND CANCER

... Retroviral insertions  Viral DNA may insert into a host chromosome in such a way that a viral promoter and response elements are next to a ...
Updated BioI_Unit3_Voc
Updated BioI_Unit3_Voc

... 19 pairs of genes that tend to be inherited together 20 frequency of crossing-over of 1% 21 genes with 3 or more alleles 22 chromosomal mutation in which a chromosome fails to separate from its homologue in meiosis 23 diagram showing how a trait is inherited over several generations 24 genetic mutat ...
DNA–DNA hybridisation
DNA–DNA hybridisation

... have the identical sequence of amino acids in their haemoglobin and so they are more closely related than humans and gibbons, which have three differences. www.mpg.de ...
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File

... They revealed how the same chemical building blocks could carry such a wide range of instructions needed for the diversity we observe in the living world. ...
4.4.1 Evidence to support the theory of evolution
4.4.1 Evidence to support the theory of evolution

... have the identical sequence of amino acids in their haemoglobin and so they are more closely related than humans and gibbons, which have three differences. www.mpg.de ...
Chapter 9 - HCC Learning Web
Chapter 9 - HCC Learning Web

MUTATION, DNA REPAIR AND CANCER
MUTATION, DNA REPAIR AND CANCER

... Retroviral insertions  Viral DNA may insert into a host chromosome in such a way that a viral promoter and response elements are next to a ...
Select one of your Biology instructors from another class and look
Select one of your Biology instructors from another class and look

... sequence of four ribonucleotides, all with equal frequency, what is the probability that any three adjacent nucleotides will be a start codon? A stop codon? In an mRNA molecule of random sequence, what is the average distance between stop codons? 8.2 If DNA consisted of only two nucleotides (say, A ...
Atlas Pfu DNA Polymerase
Atlas Pfu DNA Polymerase

Genetic engineering
Genetic engineering

... 3. The human gene is place into the bacteria plasmid 4. The plasmid is placed back into the bacteria. • The cell now has directions (DNA) to make insulin. • That's exactly what it does. • Its human insulin, bacteria do not make insulin on their own. ...
Chapter 14
Chapter 14

... DNA fragments and then cooled to allow for the primer to bind to a single strand of DNA • DNA polymerase synthesizes a complementary strand until a chemical tag is incorporated  because of the relatively low concentration of chemical tags compared with the nucleotides, the mixture will contain a se ...
The title: A Structure for Deoxyribose Nucleic Acid
The title: A Structure for Deoxyribose Nucleic Acid

... Characteristic 3: the two strands in DNA are anti-parallel. "Both chains follow right- handed helices, but owing to the dyad the sequences of the atoms in the two chains run in opposite directions." Dyad means the pairing of opposite bases. This is analogous to two teams shaking hands in two passing ...
The Central Dogma Activity (Student Sheet)
The Central Dogma Activity (Student Sheet)

... The objective of this lesson is to explore and then demonstrate the principle of The Central Dogma. By the end of the activity, you should be able to identify and explain the relationship between DNA and protein by describing the three processes involved in the Central Dogma as well as explain the p ...
Biology Final Exam Review
Biology Final Exam Review

... • each RNA codon specifies just one amino acid. • in most organisms, the same codons specify the same amino acids. • no two organisms have exactly the same DNA. ...
DNA2016 - saddlespace.org
DNA2016 - saddlespace.org

... Watson and Crick – 1953  Discovered the structure of DNA  Nobel prize in 1962 (with Wilkins)  Deduced that DNA was a double helix ...
The entire human genome consists of 23 pairs of chromosomes
The entire human genome consists of 23 pairs of chromosomes

... CHROMOSOME ...
Epigenetics - Louisiana State University
Epigenetics - Louisiana State University

... Louisiana State University ...
- Nour Al Maaref International School
- Nour Al Maaref International School

... _____ 4. Which of the following events occurs directly after RNA polymerase recognizes the transcription start site of a gene? a. The polymerase strings amino acids into a polypeptide. b. Free-floating nucleotides pair up with exposed DNA bases. c. A complementary RNA strand detaches itself from the ...
Name - PSUSDscienceresources
Name - PSUSDscienceresources

... the tongue. This is because A cells in the tongue do not contain amylase genes. B cells in the tongue do not express the amylase genes. C DNA varies from cell to cell based on the cell's needs. D cells in the tongue send amylase to the salivary gland cells. ...
BL414 Genetics Spring 2006  page Test 2
BL414 Genetics Spring 2006 page Test 2

... 1) (2.5pts) T or F: ___T_______ Bacterial genomes do not have many repetitive sequences, most of their genome is unique. 2) (2.5pts) T or F: ____F______ Genetic linkage in corn can be analyzed using asci tetrad analysis. 3) (2.5pts) T or F: _____F_____ The Holliday model is the currently accepted mo ...
Modern Taxonomy
Modern Taxonomy

... Image from: Biology by Miller and Levine; Prentice Hall Publishing ©2006 ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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