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1 - Cordis
1 - Cordis

... locked for mechanical reasons. Using massive mutagenesis and a powerful genetic screen we have been able to identify the IntI1 integrase residues involved in the attC recognition and we have been able to make a structural modeling of this integrase. In parallel, we have demonstrated that the integra ...
dna adducts - dr
dna adducts - dr

... • An adduct near (on the promotor region of) a gene may lead to over-expression of that gene. • An adduct may block DNA repair mechanisms. • An adduct may interfere with the current methylation pattern. The methylation pattern on DNA acts as a template for gene expression, blocking or allowing the e ...
Final Study Guide
Final Study Guide

... examination, it can be seen that the coat of a roan cow consists of both red and white hairs. This trait is one controlled by _____. 38. Eye color in humans is the result of _____ inheritance. 39. The blood types A, B, AB, and O are the result of _____ inheritance. 40. A child is diagnosed with a ra ...
Powerpoint
Powerpoint

... contains the information to code for one complete protein  PROTEINS are made up of a chain of amino acids  Proteins determine many of the traits in an organism ...
Chapter Three The Biological Basis of Life
Chapter Three The Biological Basis of Life

... Cellular function and an organism’s inheritance. DNA is composed of two chains of nucleotides. A nucleotide consists of a sugar, a phosphate, and one of four nitrogenous bases. The two chains are held together by bonds formed on their bases with their complement on the other chain.  Adenine (A) is ...
How are protein products made from a gene?
How are protein products made from a gene?

... are mixed together, and prior to being baked, we can equate this to the sequence of amino acids created from the RNA template. Baking the ingredients makes a cupcake, which is like the 3-D protein. So, the cell is like a bakery. Different bakeries (cells) have different recipes (genes expressed) whi ...
4-5
4-5

HIV treatments
HIV treatments

... full inclusion of viral proteins ...
Alternative Approaches to Molecular Biology
Alternative Approaches to Molecular Biology

... With a circular chromosome, the DNA is continuous – it has no "end". This means that there will always be DNA from which to make the RNA primer for the lagging strand. d) Other organisms have non-coding sequences at the ends of linear chromosomes called telomeres. A telomere is simply a long stretch ...
The Impact of Computer Technology in Molecular Biology and
The Impact of Computer Technology in Molecular Biology and

... Previous versions of BLAST did not account for ...
Impact of Computer Technology in Molecular Biology and Genetics
Impact of Computer Technology in Molecular Biology and Genetics

... Previous versions of BLAST did not account for ...
Repeated DNA sequences - lecture 1
Repeated DNA sequences - lecture 1

... Two of these (CAG and CCG) are involved in human genetic disease. In the genes that contain them, the copy number (n) of the repeat is variable. If n<40, there are no symptoms. But if n>50, symptoms of the disease start to show (these thresholds are slightly different in different diseases). In many ...
Bio 139: Exam #2 Review Outline: Wed. Nov. 1
Bio 139: Exam #2 Review Outline: Wed. Nov. 1

... phosphorlyation of a molecule such as ADP to ATP using the energy released by breaking a chemical bond) and oxidative-phosphorylation (the formation of ATP from ADP using the energy of a proton gradient across a membrane, depends on oxygen as the terminal electron acceptor in the electron transport ...
The_RAY_Manual
The_RAY_Manual

... E. coli as well as ES-cells, permitting a selection for the recombination product in E.coli. Cotransformed yeast colonies are pooled, extrachromosomal DNA is prepared and electroporated into E. coli. Bacterial transformants containing the recombination product are selected on plates containing kana ...
SBI 4UW DNA Barcoding Assignment 2015 / 50 marks
SBI 4UW DNA Barcoding Assignment 2015 / 50 marks

Interest Grabber
Interest Grabber

... encyclopedia. Suppose that you go to the library to do research for a science project. You find the information in an encyclopedia. You go to the desk to sign out the book, but the librarian informs you that this book is for reference only and may not be taken out. ...
Chapter 10- Molecular Biology of Genes
Chapter 10- Molecular Biology of Genes

... DNA (not protein) • Hershey and Chase- 1952 – Used bacteriophages- virus that infects bacteria • They are composed of DNA, RNA, protein coat • Virus adheres to bacteria and injects genetic information into it, viral genes act to produce new bacteriophages, cell bursts and new virus come out ...
Cells - SchoolRack
Cells - SchoolRack

...  Information is copied onto an mRNA. For example GCCATA from the DNA would be CGGUAU on the mRNA  (RNA has Uracil instead of Thymine.) RNA has A U C G.  If the mRNA cannot copy the message from DNA because of a bacterial or viral infection a protein cannot be made. ...
Sample Exam 1
Sample Exam 1

... one OR describe how they are named. (3 points) ...
AP Biology Objectives
AP Biology Objectives

... The Synthesis of Protein 10. Describe the structure and function of tRNA, and ribosomes. 11. Describe initiation, elongation, and termination of translation, AND explain which enzymes, protein factors, and energy sources are needed for each stage. 12. Explain what determines the primary structure of ...
Unit #3 Retake Ticket Unit 3 Retake Ticket
Unit #3 Retake Ticket Unit 3 Retake Ticket

... 1. A change in a single gene is called a _________________ mutation. a. When a nucleotide is deleted a _____________________ mutation occurs. b. When a nucleotide is added, a ______________________ mutation occurs. c. When one nucleotide is changed for another, it is called a ____________________ mu ...
Homework 1
Homework 1

... D) mutations E) analogues 2) _____ The fastest method to search for the chicken insulin gene using the human insulin gene is: A) PCR B) BLAST! C) gene amplification D) crystallography E) DNA purification 3) ________ Which of the following is a phenetic characteristic? A) these all have insulin ortho ...
DNA Puzzle Paragraph
DNA Puzzle Paragraph

... Most of the information in DNA is stored in segments called ______________. A gene is a specific sequence of nucleotides in a strand of DNA that codes for a specific polypeptide, or sequence of amino acids. Within a given molecule of double-stranded DNA, genes may reside on either of the two strand ...
1 - TeacherWeb
1 - TeacherWeb

... Which taxonomic classifications do Homo neanderthalensis and Homo sapiens share? Which do they not share? Based on this information, is it believed that the two could have interbred? If both are thought to have evolved from Homo erectus, under what conditions could they have become separate species? ...
Genetics 314 – Spring 2005
Genetics 314 – Spring 2005

... can occur. Both viruses must be within the same cell for an exchange to occur. b) Describe one method of recombination that could lead to a more virulent avian flu virus. Once both viruses are within the same cell recombination can occur through several mechanisms. The first would be a simple error ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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