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Fathers and Mothers of Genetics
Fathers and Mothers of Genetics

... (1822 – January 6, 1884) a german monk; referred to as the "father of genetics" for his study of the inheritance of traits in pea plants. Mendel showed that the inheritance of traits follows particular laws, which were later named after him. The significance of Mendel's work was not recognized until ...
Option B: Biotechnology and Bioinformatics AHL
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... Outline three outcomes of the sequencing of the complete human genome. (4.4.6) Online ...
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... 39. Describe the application of DNA profiling to determine paternity and also in forensic investigations. 40. Analyze DNA profiles to draw conclusions about paternity or forensic investigations. To do this, complete the Murder Mystery by using DNA profiling. (Will be handed out) 41. Outline three ou ...
DNA Base Pairing Activity
DNA Base Pairing Activity

... DNA Base Pairing Activity  The purpose of this activity is to reinforce the idea that base pairs only pair with certain other base  pairs.  1. Pass out the Base Pair Cards to students. It will work best if there are equal numbers of each  letter.  2. Inform the students that they should put out thei ...
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CH 14 EXTRA CREDIT Study Guide

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Ch 11- Controlling Gene Expression

... • When lactose is absent= doesn’t want to bother making the protein to break down lactose – Promoter- site where RNA pol attaches – Operator- site that determines whether promoter can bind or not to RNA pol – Promoter + operator + genes to be transcribed = operon – Repressor- protein that binds to o ...
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Today`s Plan: 4/25/03

... • 1st –cloning vector is removed from bacterial cells • 2nd-restriction enzymes that recognize specific base sequences on the DNA cut the plasmid at pre-determined sites, creating sticky ends • 3rd-the donor gene is inserted, closing the plasmid back into its ring shape • 4th-the new, recombinant DN ...
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DNA - The Double Helix
DNA - The Double Helix

... The rungs of the ladder are pairs of 4 types of nitrogen bases. The bases are known by their coded letters --- A, G, T, and C. These bases always bond in a certain way. Adenine will only bond to thymine. Guanine will only bond with cytosine. This is known as the "Base-Pair Rule." The bases can occur ...
Sequencing the Human Genome
Sequencing the Human Genome

... 2. “Shock” these into the DNA of e-coli bacteria, and let them replicate the BACs to any degree. 3. Take each BAC and cut it into manageable pieces, using restriction enzymes. 4. Clone (artificially replicate) these pieces, so as to have enough to work with. This is known as PCR, or polymerase chain ...
DNA - The Double Helix
DNA - The Double Helix

... The rungs of the ladder are pairs of 4 types of nitrogen bases. The bases are known by their coded letters --- A, G, T, and C. These bases always bond in a certain way. Adenine will only bond to thymine. Guanine will only bond with cytosine. This is known as the "Base-Pair Rule." The bases can occur ...
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... DNA fingerprinting uses more than one section of noncoding DNA. For example, five different regions of DNA might be used to make a DNA fingerprint. The more regions that are used, the less likely it is that two people will have the same DNA fingerprint. There is a very small change – in in many mill ...
DNA - The Double Helix
DNA - The Double Helix

... The rungs of the ladder are pairs of 4 types of nitrogen bases. The bases are known by their coded letters --- A, G, T, and C. These bases always bond in a certain way. Adenine will only bond to thymine. Guanine will only bond with cytosine. This is known as the "Base-Pair Rule." The bases can occur ...
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... DNA Synthesis The synthesis of new DNA strands complementary to both strands of the parental molecule posed an important problem to understanding the biochemistry of DNA replication. Since the two strands of double-helical DNA run in opposite (antiparallel) directions, continuous synthesis of two ...
Exploring Mutant Organisms Teacher Extended Background
Exploring Mutant Organisms Teacher Extended Background

... Thomas Hunt Morgan, who worked at Columbia University in the early 1900s, was the first researcher to use the fruit fly, Drosophila melanogaster, in the study of gene inheritance patterns. Fruit flies are tiny insects that feed on a fungus that grows on fruit, and they are an excellent species choic ...
Topic 20 revision notes - Mr Cartlidge`s Saigon Science Blog
Topic 20 revision notes - Mr Cartlidge`s Saigon Science Blog

... insertion of human DNA into bacterial plasmid DNA using DNA ligase to form a recombinant plasmid ...
Things to Know for the Test – Honors
Things to Know for the Test – Honors

... your answer, the process of transcription, translation, what occurs during each, why the processes are read the way they are, where they occur in the cell, etc. DNA is the blueprint of life. It is made of nucleotides that contain the code to make proteins. Proteins control everything that an organis ...
Topic 3 The chemistry of life
Topic 3 The chemistry of life

... 47. To replicate a molecule of DNA must unwind to expose the nitrogenous bases. 48. Helicase is the enzyme that breaks the hydrogen bonds to allow the unwinding. 49. The exposed bases of each strand are then paired with an available nucleotide by complementary base pairing. The result is two strands ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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