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Microbial Genetics - Austin Community College
Microbial Genetics - Austin Community College

... to help the nucleotides begin to bind. The complementary bases are then added to the template (parent) strand using an enzyme called polymerase. – DNA can only replicate in the 5’to 3’ direction. The reason is because the chemical group on 3’ side of the nucleotide acts like a hand that can grab ont ...
19GeneticEngineering
19GeneticEngineering

... hormone and clotting factors that were all once rare and expensive. This is done by transforming cells by inserting a human gene into the cell of a bacterium. Remember: Way to high joke. ...
Chapter 11
Chapter 11

... • Mutations can alter the genetic message and affect protein synthesis. • The effect of a mutation depends on the identity of the cell where it occurs. • Mutations in germ-line cells - will be passed to future generations • Important for evolutionary change • Mutations in somatic cells are not passe ...
Transcription Protein Synthesis So what does it mean? Transcription
Transcription Protein Synthesis So what does it mean? Transcription

... 4. Only a small part of the DNA double helix is unwound/unzipped at a time – RNA polymerase travels along the gene, bringing in RNA nucleotides to base-pair with the existing DNA nucleotides along the 3’  5’ leading strand, called the template ...
Abstract
Abstract

1. Discuss the contributions of Mayer.
1. Discuss the contributions of Mayer.

... replication, which begins at a single origin on the chromosome It is an asexual process, producing clones 2 replication forks move bi-directionally until they meet and replication is complete Bacteria can divide every 20 minutes  ...
TRANSGENIC ANIMALS
TRANSGENIC ANIMALS

... o Rabbits are quite promising for gene farming or molecular farming, which aims at the production of recoverable quantities of biologically important proteins encoded by the transgenes. o Transgenic animals used for this purpose are popularly called bioreactors. o These transgenes are expressed in m ...
Lecture 8 slides
Lecture 8 slides

... Genetic linkage Human pedigrees Tetrad analysis ...
Vectors
Vectors

... it can be introduced into the desired host cells such as mammalian, yeast, or special bacterial cells. The host cells will then synthesize the foreign protein from the recombinant DNA. When the cells are grown in vast quantities, the foreign or recombinant protein can be isolated and purified in lar ...
Annex A: Highlights of the “Biotechnology Revolution”: 1953–present 1953 Nature
Annex A: Highlights of the “Biotechnology Revolution”: 1953–present 1953 Nature

... short genetic region of a particular bacterial virus. Over a five-year period, Benzer mapped recombinations of genetic material that distinguished mutational changes that had taken place at ...
Works Cited - WordPress.com
Works Cited - WordPress.com

...  Why does the DNA separate? (DNA non-soluble in rubbing alcohol; forms precipitate) Lesson Conclusion  Look at strands of DNA from the experiment; inform that if we were to look at it under a microscope we would see the strands better  Questions o Why do you think the Strawberry was a good sample ...
2 Exam paper_2006[1] - University of Leicester
2 Exam paper_2006[1] - University of Leicester

... 1. Describe a strategy that could be employed to produce a representative genomic library that could be screened to identify a functional bacterial gene. Highlight any features that would be useful in the cloning vector used to make the library. 2. Why is DNA replication semi-discontinuous? ...
GENE EXPRESSION CH 17
GENE EXPRESSION CH 17

... http://highered.mcgrawhill.com/sites/0072556781/student_view0/chapter11/animation_quiz_3.html http://highered.mcgrawhill.com/sites/0072556781/student_view0/chapter11/animation_quiz_4.html https://www.youtube.com/watch?v=kp0esidDr-c http://highered.mcgrawhill.com/sites/0070960526/student_view0/chapte ...
DNA
DNA

...  Concluded that when the S cells were killed, DNA was released  R bacteria incorporated this DNA into their cells and changed into S cells. ...
Slide 1
Slide 1

... One reason that prokaryotes have been such valuable model organisms in genetic studies is that their generation time is so short. In fact, some species can reproduce as often as every 20 minutes. This gives the researcher answers literally over night. With this in mind, which of the following eukar ...
Genetics Review Sheet
Genetics Review Sheet

... Resources: DNA worksheet, DNA extraction lab, DNA quiz  What is a gene?  Where are chromosomes located?  2 main scientists that established the structure of DNA?  Female scientist who paved the way for them?  Shape of DNA?  Sugar of DNA?  4 bases of DNA?  What pairs with what?  How does it ...
File
File

... interlocking pieces of puzzle). - Adenine always pair with Thymine - Guanine always pairs with Cytosine 4. Genes: is a section on the DNA. Genes contain instructions for making specific protein. - Each gene contains hundreds or thousands of amino acids (the building blocks of protein). - The gene de ...
6.G Meiosis Graphic Organizer 6.H Genetic Variation
6.G Meiosis Graphic Organizer 6.H Genetic Variation

EOC Review 2 - Wayne County Public Schools
EOC Review 2 - Wayne County Public Schools

... These pairs line up during synapsis of prophase 1 of meiosis and gene shuffling occurs in the process called ...
Inherited traits are traits that you get from your parents
Inherited traits are traits that you get from your parents

... 20) DNA has the ability to make an exact copy of itself. Draw and explain how DNA Replicates. Why is this ability important for life to continue? The DNA molecule splits apart and each ½ strand of DNA is used as a template to make a new molecule. Each new DNA molecule is an exact copy of the origina ...
Genetics Exam 5
Genetics Exam 5

... Problems (3 points each) You want to design an oligonucleotide probe to identify a clone containing a new enzyme that you purified. You determine that the amino terminal sequence of your enzyme is: MCFYMDW What should be the sequence of the oligonucleotide probe? Indicate redundancy by putting all ...
From Gene to Protein—Transcription and Translation
From Gene to Protein—Transcription and Translation

... 18. What part of translation depends on the base-pairing rules? 19. Explain why it makes sense to use the word translation to describe the change from mRNA to a protein. 20. To summarize what you have learned about translation, explain how an mRNA molecule directs the synthesis of a protein. Include ...
Human genome study reveals certain genes are less essential than
Human genome study reveals certain genes are less essential than

Lecture 2 Nucleic Acid Structure
Lecture 2 Nucleic Acid Structure

... Melting Temperature Tm -Tm characterizes the stability of double helix ...
Final Review Answer Key - Mercer Island School District
Final Review Answer Key - Mercer Island School District

... A bacterium (plural bacteria) is a microorganism made of a prokaryotic cell, which is a cell that does not contain a nucleus or other membrane-bound organelles. The DNA for the bacterial cell is found in the cytoplasm of the cell (without a nuclear membrane surrounding it. Bacteria have a circular c ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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