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Model for crossing over and gene conversion
Model for crossing over and gene conversion

... on the heavy chromatid and the GC bases on the light chromatid There is a two strand break within one double helix; the arrow heads are the 3' ...
Viral Replication - Hartland High School
Viral Replication - Hartland High School

... 3 Synthesis of viral genomes and proteins. The phage DNA directs production of phage proteins and copies of the phage genome by host enzymes, using components within the cell. ...
protein synthesis
protein synthesis

... The steps in this part of the process are: 1. RNA polymerase binds to DNA strand and unwinds a short section (about 12 base pairs long) 2. This then travels along the DNA strand building an RNA molecule from the TEMPLATE STRAND ...
Name:
Name:

...  Dominant vs. Recessive; Heterozygous v. homozygous; genotype v. phenotype; trait vs. gene/allele  Monohybrid crosses; be able to do them.  Complex patterns of inheritance: incomplete & co-dominance, polygenic traits, multiple alleles, sex-linkage; know examples of each.  Autosomes vs. sex chrom ...
Unit 4
Unit 4

... Distinguish between heterochromatin and euchromatin.  Heterochromatin is a nontranscribed eukaryotic chromatin that is so highly compacted that it is visible with a light microscope during interphase. ...
Rosa blanda
Rosa blanda

... The objective of this project was to determine the genus, and if possible the species, of several berry-bearing trees growing in the Peconic River System by properly barcoding the leaves and berries and and compare the results using the DNA Subway website and the NCBI BLAST tool . Out of the 17 samp ...
Chapter 2 Genes Encode RNAs and Polypeptides
Chapter 2 Genes Encode RNAs and Polypeptides

... 2.2 Most Genes Encode Polypeptides • heteromultimer – A molecular complex (such as a protein) composed of different subunits. • homomultimer – A molecular complex (such as a protein) in which the subunits are identical. • Some genes do not encode polypeptides, but encode structural or regulatory RN ...
Name: Period: REVIEW FOR FINAL EXAM Topic/Concept What you
Name: Period: REVIEW FOR FINAL EXAM Topic/Concept What you

... coming from one common ancestor is called ____ (two different terms) 14. How do bacterium becomes “resistant” to an antibiotic? 15. What are analogous structures? ...
DNA, Genes and Chromosomes
DNA, Genes and Chromosomes

Chapter 2 Genes Encode RNAs and Polypeptides
Chapter 2 Genes Encode RNAs and Polypeptides

... 2.2 Most Genes Encode Polypeptides • heteromultimer – A molecular complex (such as a protein) composed of different subunits. • homomultimer – A molecular complex (such as a protein) in which the subunits are identical. • Some genes do not encode polypeptides, but encode structural or regulatory RN ...
PowerPoint Presentation - Documented Gene Transfer in Bacteria
PowerPoint Presentation - Documented Gene Transfer in Bacteria

... Infection of Host Cells by Phages • Adsorption – LPS for T4 • Irreversible attachment • Sheath Contraction • Nucleic acid injection • DNA uptake ...
Section 18.1 Summary – pages 475-483
Section 18.1 Summary – pages 475-483

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Cryptography and Linguistics of Macromolecules Cryptography and
Cryptography and Linguistics of Macromolecules Cryptography and

... Any kind of information can undergo MSA, as long as it can be modelled as a sequence of symbols of a finite alphabet. The best-known example of such modelling are DNA sequences, whose own physical constitution can be immediately translated to a sequence of letters. Applying MSA techniques to these s ...
Welcome to DNA Replication 101
Welcome to DNA Replication 101

... Welcome to DNA Replication 101 If one cell is going to divide to produce two new cells, the first cell must copy all of its parts before it can split in half. The cell grows, makes more organelles, and copies its genetic information (the DNA) so that the new cells each have a copy of everything they ...
DNA review worksheet.. - hrsbstaff.ednet.ns.ca
DNA review worksheet.. - hrsbstaff.ednet.ns.ca

... 27. What acts as the template in DNA replication? 28. What is a replication fork? 29. What enzymes help separate the 2 strands of nucleotides on DNA? What bonds do they break? 30. What is the function of DNA polymerases? 31. ____________________ are joined to replicating strands of DNA by __________ ...
Epigenetics and Inheritance
Epigenetics and Inheritance

...  Epigenetics is generally defined “as relating to or arising from ...
Bacterial Growth and Reproduction
Bacterial Growth and Reproduction

... Generation Time: The time required for a cell to divide or a population to double • Most bacteria have a doubling time of 1-3 hours, although some may be greater than 24 hours • Example: E. coli has a doubling time of 20 minutes • Bacterial division occurs according to a ...
DNA, Mitosis & Meiosis
DNA, Mitosis & Meiosis

... • Mitochondria have their own DNA and replicate themselves. ...
Powerpoint slides - School of Engineering and Applied Science
Powerpoint slides - School of Engineering and Applied Science

... • Start with two cell types, e.g. “healthy” and “diseased”. • Isolate mRNA from each cell type, generate cDNA with fluorescent dyes attached, e.g. green for healthy and red for diseased. • Mix the cDNA samples and incubate with the microarray. • After incubation the cDNA in the samples has had a cha ...
GENETIC TECHNOLOGY
GENETIC TECHNOLOGY

... plate with bacterial cells containing recombinant vector with different pieces of chromosomal DNA  Use single-stranded DNA probe  Dark spots correspond to colonies with gene of interest ...
Biotechnology
Biotechnology

... discovery of restriction enzymes Restriction enzymes: enzymes that cut DNA at specific recognition nucleotide sequences known as restriction sites They were discovered in bacteria & are thought to have evolved to provide a defense mechanism against invading viruses ...
Additional Glossary and Concepts List for Vertebrate Genetics
Additional Glossary and Concepts List for Vertebrate Genetics

Red/ET Recombination
Red/ET Recombination

... Targeting constructs to humanize animal models Replace a given exon with the human counterpart to study the influence of your drug on a human-derived allele. ...
PCR and Its Applications
PCR and Its Applications

... What is PCR? : The “Reaction” Components 1) Target DNA - contains the sequence to be amplified. 2) Pair of Primers - oligonucleotides that define the sequence to be amplified. 3) dNTPs - deoxynucleotidetriphosphates: DNA building blocks. 4) Thermostable DNA Polymerase - enzyme that catalyzes the re ...
Chapter 12. Regulation of the Cell Cycle
Chapter 12. Regulation of the Cell Cycle

... cycle is so key to life that the genes for these regulatory proteins have been highly conserved through evolution  the genes are basically the same in yeast, insects, plants & animals (including humans) ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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