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DNA
DNA

... population numerous possibilities exists for the number of times a particular sequence of base letters can repeat itself on a DNA strand. Possibilities become greater when one deals with two ch. Each containing different lengths of repeat sequ. ...
Chapter 5
Chapter 5

... Stem Cells: Embryonic and Adult • Embryonic stem cells are undifferentiated cells in the early animal embryo that give rise to specialized cells. Grown in the laboratory, certain growth factors can induce changes in gene expression so that the cells may develop into a certain cell type. • Adult ste ...
Nucleotides Base Pair By Hydrogen bonds
Nucleotides Base Pair By Hydrogen bonds

... synthesis. Cells that have stopped cycling, such as muscle and nerve cells, are said to be in a special state called Go. • S phase is the period of time during which DNA replication occurs. At the end of S phase, each chromosome has doubled its DNA content and is composed of two identical sister chr ...
DNA- The Genetic Material
DNA- The Genetic Material

... two, non-homologous chromosomes. – Ex: Alagille syndrome results from a deletion of chromosome 20 or a translocation that disrupts an allele on chromosome 20. – Distinctive face, abnormalities of eyes & internal organs, and severe itching. ...
Biology Fall Final Review 2015
Biology Fall Final Review 2015

... a.The amino acid sequence would be shorter than expected. b.The identity of one amino acid would change. c. The amino acid sequence would remain unchanged. d.The identities of more than one amino acid would change. 97. Which type of mutation usually has the most significant impact on the protein bei ...
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Biology Study Guide

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KlenTherm™ DNA Polymerase
KlenTherm™ DNA Polymerase

... KlenTherm™ DNA Polymerase is thermostable polymerase corresponding to the KlenTaq Polymerase described by W. M. Barnes. It is a N-terminally truncated Taq DNA polymerase. As expressed from a gene construct in E.coli, translation initiates at Met236, bypassing the 5'-3' exonuclease domain of the DNA ...
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Issues in Biotechnology

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ppt - Department of Plant Sciences

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DNA Sequencing

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... The DNA molecule are in long thin strands, the strands are in pairs and two strands are joined by cross links like the steps in a ladder. The strands are also coiled in a double spiral (double helix) these strands are the chromosome and small sections of it are known as genes. When egg and sperm uni ...
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Study Guide: Meiosis and Genetics

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... detection. However, by designing chemical specificity at or near the protein channel, the residence time of different molecules can report not only the presence, but also the type of molecule that is present in the analyte buffer. Focusing on commonly known catecholamine neurotransmitters, ...
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... process of binary fission, as each new cell must have an accurate and complete copy of the DNA in order to function properly. Although prokaryotic DNA often appears as a tangled mass, it is in fact organized as a single, supercoiled circular chromosome. Replication of the circular bacterial chromoso ...
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BIO101 Objectives Unit3 Blinderman Mercer County Community
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... 10. Explain why males have 24 linkage groups while human females exhibit 23 groups 11. Relate crossing over between homologous non-sister chromatids during meiosis to genetic recombination 12. Correlate non-disjunction of chromatids during meiosis to chromosomal number changes in offspring 13. Provi ...
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... 2) When a cell puts its genetic code into action it is making precisely the proteins it needs for its structure and function. 3) If something changes a gene’s code words, the resulting protein may change. 4) If the protein is central to the cell’s architecture then a mutation could result in an abno ...
Cellular Control
Cellular Control

... Culturing host cells Not all cells take up the genes  Need to able to select bacteria that have taken up the gene.  Use plasmids that carry genes that are resistant to antibiotics  When grown with media containing antibiotics only those with the gene will then grow ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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