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PowerPoint Presentation - Gene Linkage and Genetic Mapping
PowerPoint Presentation - Gene Linkage and Genetic Mapping

... • The nicked strands unwind, switch partners, forming a short heteroduplex region with one strand and a looped-out region of the other strand called a D loop • The juxtaposed free ends are joined together, further unwinding and exchange of pairing partners increase the length of heteroduplex region— ...
File - South Waksman Club
File - South Waksman Club

... 3a. Resuspend the cell pellet Resuspend the bacterial pellet in 200 µl of Solution I by pipeting up and down. Add 200 l of Solution I, cap the tube, and vortex on the highest setting (pipetman can be used). Look very closely for any undispersed pellet before proceeding to the next step. It is esse ...
Advanced Molecular Biology and Biotechnology
Advanced Molecular Biology and Biotechnology

... uses of gene/chromosome libraries (human, animal and plant gene libraries) as well as restriction fragment length polymorphism (RFLP) analysis will be covered under this module. Bacterial expression systems are the most commonly used in biotechnology therefore a component of the course will focus on ...
The Scientific Method in Biology
The Scientific Method in Biology

... from one of the DNA strands Process is called transcription Primary point of control of gross levels of gene products Base-pairing controls the specificity RNA is unstable and transient compared to DNA RNA can be used directly or be an intermediate for protein production ...
Mitosis
Mitosis

... 9. Crossing a pure-bred green-podded (dominant trait) plant with a pure-bred yellow-podded (recessive trait) plant is symbolized by: GG x gg 10. When Gregor Mendel crossed true-breeding tall plants (TT) with true-breeding short plants (tt), the offspring were tall (Tt) because the allele for tallnes ...
TM Review Genetics
TM Review Genetics

... homozygous dominant father and a heterozygous mother. Describe the genotypes and phenotypes of the offspring, including the percentages of each. ...
Notes
Notes

... the negative phosphates from each other. Otherwise the DNA would repel itself and could not bend. The tight packing can make the DNA more inaccessible to transcription. Transcription factors which need to gain access via the major groove normally so they can read and interact with a particular base ...
2. Be sure that your exam has 9 pages including this cover sheet.
2. Be sure that your exam has 9 pages including this cover sheet.

... Choose the best answer for the question or the best ending for each statement. Write the letter (A-E) which corresponds to the best answer on the line before the question. (2 pts each) _____1. A key point in Darwin's explanation of evolution is that A. biological structures most likely inherited are ...
DNA fingerprinting Genes and DNA
DNA fingerprinting Genes and DNA

... DNA Fingerprinting - STR • Benefits – At least 13 loci are used which assort independently. • High degree of accuracy based on statistics • The probably of a particular combination of these 13 loci is one in a quintillion (1 with 18 zeros after it). • This means that it is statistically impossible f ...
ibbiochapter3geneticsppt(1)
ibbiochapter3geneticsppt(1)

... sequence 1-valine-histidine a)_________b)________c)_______d)_________-glutamic acid • sequence 2-valine-histidine e)_________f)_________g)_______h)________glutamic acid • use genetic code to solve the above • this will change the structure of resulting protein-mutation ...
File - Schuette Science
File - Schuette Science

... Removed from a chromosome Added to a chromosome Rearranged within a chromosome Switched with DNA from another chromosome. ...
Biotechnology
Biotechnology

... pick up naked foreign DNA wherever it may be hanging out  have surface transport proteins that are ...
The chemical basis of heredity Nucleic acid
The chemical basis of heredity Nucleic acid

... The chemical basis of heredity Nucleic acid: is the chemical material that carry genetic information in all types of living organism. There are two types of nucleic acid; 1. Deoxy Ribo Nucleic Acid (DNA):this type found in the nucleus and in all type of living organism except in virus. 2. Ribos Nucl ...
High-Efficiency DNA Topoisomerase I PCR Cloning
High-Efficiency DNA Topoisomerase I PCR Cloning

Protein Synthesis-Part Two - Halton District School Board
Protein Synthesis-Part Two - Halton District School Board

... • The new sequence may not produce a viable organism, depending on which genes are reversed. • Advantageous characteristics from this mutation are also possible ...
1 Mbp DNA for human genome
1 Mbp DNA for human genome

... forensic analysis homozygous vs. heterozygous state for a particular microsatellite locus? ...
Plate 32 - Viral Replication
Plate 32 - Viral Replication

... • There are viruses that infect every kind of organism: animals, plants, fungi, bacteria, ...
The Genetic Science Glossary - Canadian Council of Churches
The Genetic Science Glossary - Canadian Council of Churches

... on the expression of a desired trait. Example: If a farmer wants to grow bigger tomatoes, planting only the seeds of the biggest tomatoes the farmer grows might select the tomatoes with the genes that cause them to be larger. This could result in a crop of tomatoes that are bigger. ...
LN #23
LN #23

... undergoes mitosis, the new cells will also have the mutation. ...
10-DNA-TranslationControl
10-DNA-TranslationControl

...  The lac operon is also regulated by an activator  The activator is a protein called CAP  It binds to the CAP-binding site and gives the RNA polymerase more access to the promoter  However, a “low glucose” signal molecule has to bind to CAP before CAP can bind to the DNA ...
Ch 12-4 - Latona
Ch 12-4 - Latona

... • Usually result in killing that body cell only. • Usually do not affect the individual. – Exception: If the mutation occurs in the DNA that controls the cell cycle (division), it can result in cancer. ...
Bacterial Transformation with (pGLO Plasmid)
Bacterial Transformation with (pGLO Plasmid)

... • Observe how genes are regulated ...
Teacher`s Notes - University of California, Irvine
Teacher`s Notes - University of California, Irvine

... genetic diseases, and to solve paternity cases. Samples can be obtained from any DNA -containing tissue or body fluid, including cheek cells, blood, skin, and hair.  In many analyses, polymerase chain reaction (PCR) is used to amplify specific regions of DNA that are known to vary among individuals ...
DNA, RNA, & Meiosis Review
DNA, RNA, & Meiosis Review

... • Haploid means having a single chromosome (only from one parent) • Humans have 23 pairs of chromsomes for a total of 46 chromosomes – this is diploid • Human gametes (eggs, sperm) have 23 chromosomes (therefore when egg and sperm combine – a cell is produced with 46 chromosomes or 23 pairs of chrom ...
CHAPTER 11.1
CHAPTER 11.1

... DNA Polymerase- makes the covalent bonds between the nucleotides ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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